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Citations for Takara Bio :
1651 - 1700 of 2788 citations for 6 4 METHOXY PHENYL 3 METHYL IMIDAZO 2 1 B THIAZOLE 5 CARBALDEHYDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: We quantified mRNA expression levels of IL-10 and chemokines identified in the RNA-Seq analysis using 2-step real-time RT-PCR (Thermal Cycler Dice Real Time System, Takara Bio). The ribosomal protein L13a (RPL13A ...
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bioRxiv - Biochemistry 2022Quote: ... cells were transfected with plasmids expressing either SARS-COV-2 S protein WT or mutants (E1182K, L1193G, E1182K/L1193G, L1182K/L1186G/L1193G) by using Calcium phosphate transfection kit (Takara-Bio). 24 h post transfection ...
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bioRxiv - Microbiology 2021Quote: ... Then PCR application using specific primer pairs (Supplemental Table 2) designed for H7N9 virus and Takara One-step RT-PCR kit (Takara, China) segment by segment ...
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bioRxiv - Microbiology 2021Quote: ... The copy number of viral RNA was standardized with a SARS-CoV-2 direct detection RT-qPCR kit (Takara, cat# RC300A). The fluorescent signal was acquired using a QuantStudio 3 Real-Time PCR system (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: ... whereas a TOPBP1 fragment corresponding to residues 2-1523 was amplified from pCDNA5-FRT/TO-LacR-FLAG-TopBP1 and cloned into pGBKT7 (Clontech/Takara) to create a fusion with the GAL4 DNA binding domain using the NdeI and XmaI sites ...
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bioRxiv - Genetics 2019Quote: ... The pure linearized vector and the elpc-2 promoter were fused using an In-Fusion HD Cloning Kit (Takara, Kusatsu, Japan) to make the elpc-2p::GFP transcriptional reporter construct ...
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bioRxiv - Molecular Biology 2019Quote: ... with a universally tailed poly-dT primer and a template switching oligo followed by amplification for 12 cycles with the Advantage 2 DNA Polymerase (Takara Bio). After ultrasonic shearing (Covaris LE220) ...
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bioRxiv - Immunology 2021Quote: ... was used to clone SARS-CoV-2 S gene into the SFG backbone using the In-Fusion Cloning kit (Takara Bio). pSFG-SARS-CoV-2 S D614G was cloned from pSFG-SARS-CoV-2 S plasmid using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs) ...
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bioRxiv - Microbiology 2021Quote: ... real-time RT-PCR was performed with SARS-CoV-2 N primer sets and SYBR Premix Ex Taq II (TaKaRa-Bio) using a LightCycler Nano (Roche ...
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR reactions were performed in a 25μl-reaction mixture containing 12.5μl of 2 × Gflex PCR Buffer (Mg2+, dNTP plus) (TaKaRa Bio Inc., Shiga, Japan), 0.5μl of Tks
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bioRxiv - Physiology 2021Quote: ... Frozen placental biopsies (25mg) were homogenized using a 2 ml Kimble dounce tissue grinder and nuclei pellets resuspended in Nuclei Suspension Buffer (Clontech #2313A) before filtering through a 40μm cell strainer ...
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bioRxiv - Microbiology 2021Quote: ... The copy number of viral RNA was standardized with a SARS-CoV-2 direct detection RT-qPCR kit (Takara, Cat# RC300A). The fluorescent signal was acquired using a QuantStudio 3 Real-Time PCR system (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... foetal bovine serum or 10% (v/v) TET System–approved FCS for U–2–OS reporter cell lines (631106, Takara Bio). U–2–OS-pEP15 cells (5 ...
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bioRxiv - Molecular Biology 2020Quote: Yeast-2-hybrid assays were performed with the plasmids and strains from the Matchmaker Gold Yeast Two-Hybrid System (Takara Bio) according to manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ... Double-strand cDNA was amplified by long-distance PCR (LD-PCR) using an Advantage 2 PCR kit (Clontech, cat. no. 639206).
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bioRxiv - Microbiology 2021Quote: ... The amplified PCR product was verified by sequencing and then cloned into modified XW55 yeast dual expression vector65 in the highlighted SpeI and PmlI sites downstream of the ADH2 promoter by yeast recombinase technology [protocolYeastmaker™ YeastTransformation System 2 (Clontech)] and according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... serving as homology arms for directional cloning of the library into RD-seq plasmid (zfh1-DSCP-Gal4-DBD, Suppl. Table 2) vector using In-Fusion HD (Clontech 639650).
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bioRxiv - Genomics 2022Quote: The genomic DNA of Mir-0 accession (ID 8337) was used as a template (2 ng) for amplification using PrimeSTAR® GXL DNA Polymerase (TaKaRa), in a 40-µl PCR reaction with 0.3 µM primers OP009 and OP010 ...
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bioRxiv - Microbiology 2022Quote: ... These fragments were cloned into pUC19 with the HYG resistance cassette upstream of pCTR4-2 with InFusion cloning (Takara, Cat#638911). Plasmids were isolated from individual clones and confirmed by digestion with HindIII (New England Biolabs ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... in a 20 µL reaction consisting of 10 μL of TB Green Premix Ex Taq 2 (TliRNaseH Plus; TaKaRa, Dalian, China), 1 µL each of forward and reverse primer ...
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bioRxiv - Plant Biology 2022Quote: ... Each of the up-regulated genes (MpGSTF7, MpGSTF10, MpGSTF15, MpCYP813A5, MpCYP822A1) was PCR amplified from genomic Tak-2 DNA using CloneAmp HiFi Premix (Takara Bio). The primers used to amplify gene sequences are listed below ...
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bioRxiv - Biochemistry 2022Quote: ... KM881710.2) were PCR amplified from viral cDNA templates (31–33) and cloned into pSUMO expression plasmid by IN-FUSION (Takara Bio). The final constructs were confirmed by sanger sequencing performed by Genewiz.
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bioRxiv - Genomics 2023Quote: ... A total of 3.5 μl ribo-depleted material was used for SMARTer (SMARTer PCR cDNA Synthesis Kit, catalog num. 634926 and Advantage 2 PCR kit, catalog num. 639207, Clontech-Takara) protocol ...
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bioRxiv - Biochemistry 2022Quote: ... cDNAs were generated by PCR amplification in 50 µl-total reaction volume using the Advantage 2 PCR Enzyme System (Clontech, #639206). PCR reaction was performed using 20 µl cDNA from previous step ...
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bioRxiv - Immunology 2023Quote: ... Retroviral supernatants were then collected and spun at 2,000g for 2 h at 32 °C onto 24-well non-tissue-culture-treated plates coated overnight in Retronectin (Takara Bio) or spinfected for 90 minutes at 32°C in 1 µcg mL-1 polybrene ...
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bioRxiv - Neuroscience 2024Quote: ... Titration of the concentrated viruses was performed by AAVpro Titration Kit (for Real Time PCR) Ver.2 (6233, Takara Bio Inc) following the manufacture’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... CMV promoter_OsTIR1-T2A-BSR_IRES_mScarlet-mAIDCENP-T fragment was cloned into the plasmid containing approximately 2 kb genome homology arm around the start codon of PGK by In-Fusion® HD (TAKARA) (pBS_PGK N-KI_ pCMV-OsTIR1-T2A-BSR-IRES-mScarlet-mAID-CENP-T).
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bioRxiv - Neuroscience 2024Quote: ... pRC2-mi342 used to produce the AAV2/2 vector was a plasmid included in the AAVpro Helper Free System (Takara Bio). The AAV2/9 plasmid was kindly provided by James M ...
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bioRxiv - Biochemistry 2024Quote: ... Fragment (∼50 ng) and linearised vector (∼50 ng) were combined with 2 µL 5X In-Fusion HD Enzyme (Takara Bio, #938910) and mixture made up to 10 µL with Nuclease Free Water (Fisher Bioreagents ...
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bioRxiv - Cell Biology 2020Quote: ... Protein samples were run on a 4-12% Bis-Tris gel (Novex) and Western blots were performed with the following antibodies: α-GFP (Clontech Living Colors 632381 (JL-8); RRID:AB_2313808 ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated in primary antibody (1:1,000 mouse anti-calbindin, Sigma; 1:500 rabbit anti-DsRed, Clontech; 1:1,000 guinea pig anti-vGluT1 ...
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bioRxiv - Molecular Biology 2024Quote: ... 4.9 μg DNA was transfected per dish containing a 1:1 molar ratio of pmCherry-N1 (Clontech) and of the pUC18-GFP or linear GFP substrate ...
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bioRxiv - Developmental Biology 2023Quote: ... primary antibodies (chicken anti-GFP, Abcam, ab13970, 1:2000 and rabbit anti-DsRed, Clontech, 632496, 1:200) were incubated overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... 1-st PCR (PCR 1) was performed using Titanium Taq DNA Polymerase (# 639209, Takara Bio, CA, USA). Separation of the PCR products from primers and gel purification was done by QIAquick PCR & Gel Cleanup Kit (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... pLKO.1-shFEN1 or pLKO.1 control vector was simultaneously transfected into LentiX293T (Cat# 632180 Clontech, Japan) with packaging plasmid ...
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bioRxiv - Cell Biology 2020Quote: ... the PLIN2 cDNA was inserted on the 5’ end of pME-tdTOMATO using In-Fusion HD Cloning Plus (Takara, Mountain View, USA, Catalog #638920). Gateway cloning using the Gateway LR Clonase Enzyme mix (Thermo Fisher ...
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bioRxiv - Plant Biology 2023Quote: ... The FW2.2 full-length coding sequence was amplified from cDNAs prepared from tomato (cv. AC) fruits at 5 DPA using PrimeSTAR MAX DNA polymerase (TAKARA BIO Inc., Kusatsu, Japan) and primers including the attB sites (Supplemental Table 1) ...
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bioRxiv - Cell Biology 2020Quote: ... Verification of positive colonies was achieved by co-transforming wild-type or YR-mutant TMEM39A loop domain (in pGBKT7 Vector) with genes of interest (in pGADT7 Vector) following the instruction of YeastMaker™ Yeast Transformation System 2 (Clontech, 630439) as well as plasmids from re-cloned cDNA.
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bioRxiv - Microbiology 2021Quote: ... The SARS-CoV-2 Spike sequence was cloned into the linearized pVSV-eGFP-dG by the In-Fusion cloning system (Takara Bio Inc.). The resulting pVSV-eGFP-deltaG_SARS-CoV-2 Spike vector was verified by sanger sequencing ...
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bioRxiv - Systems Biology 2019Quote: ... The relative 2 standard curves real-time PCR was carried out on the cDNA samples using TaqMan master mix (Takara, Otsu, Japan) and a Q-6000 machine (Qiagen ...
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bioRxiv - Genomics 2021Quote: ... were used for library construction using the SMARTer Stranded Total RNA-Seq Kit v.2 (634418, Pico Input Mammalian, Takara/Clontech, Japan) according to the manufacturer’s protocol without RNA fragmentation ...
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bioRxiv - Plant Biology 2021Quote: ... SlGA2ox6 and SlGID1b-1 were constructed into pAbAi plasmid and transformed into Y1HGold yeast strain under the guidance of Yeastmaker™ Yeast Transformation System 2 (TAKARA, Japan). The recombinant yeast strain was confirmed by PCR conducted with Matchmaker™ Insert Check PCR Mix (TAKARA ...
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bioRxiv - Biochemistry 2020Quote: ... was transformed with the appropriate plasmids (see figure legends) according to the manufacturer’s instructions using the lithium acetate procedure (Clontech Matchmaker 2 manual). Liquid cultures were grown overnight in standard dropout (SD ...
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bioRxiv - Synthetic Biology 2021Quote: ... The amplified fragments were cloned into a modified pET16b expression vector (Table 2) by using In-Fusion cloning kit (Takara, Shiga, Japan). The sequence of the inserts of the resulting plasmids was verified by Sanger sequencing ...
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bioRxiv - Microbiology 2022Quote: ... cDNA was prepared from 2 µg of each RNA sample using the PrimeScript II 1st strand cDNA synthesis kit (TaKaRa, Shiga, Japan) with 40 units of RNasin Plus RNase inhibitor (Promega ...
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bioRxiv - Immunology 2020Quote: Transferrin over-expression or knockdown vectors were constructed and HEK 293T cells (Conservation Genetics CAS Kunming Cell Bank, China) and EcoPack™ 2–293 cells (Clontech, USA) were used to package lentiviruses and retroviruses ...
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bioRxiv - Molecular Biology 2019Quote: The qRT-PCR analysis was conducted in a total volume of 20μL containing 10μl 2 × SYBR® Premix Ex TaqII (Tli RNaseH Plus) (Takara Bio Inc., China), combined with sense and antisense primers (0.8 μl ...
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bioRxiv - Genetics 2020Quote: ... Verification of positive colonies was achieved by co-transformation of extracted bait and prey plasmids following the instruction of YeastMaker™ Yeast Transformation System 2 (Clontech, 630439) and by bait/prey plasmids with re-cloned cDNA.
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bioRxiv - Genetics 2020Quote: ... First-round reverse transcription PCR (RT-PCR) was conducted by using PrimeScript One Step RT-PCR Kit Ver.2 (TaKaRa, Dalian, China). A 10 μL reaction mixture contained 5 μL of 2 × 1 Step Buffer ...
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bioRxiv - Immunology 2021Quote: Retroviral SARS-CoV-2 Spike pseudovirus were generated in 293T cells by co-transfecting expression plasmids containing SARS-CoV-2 Spike and MLV gag/pol and luciferase vectors using Calphos transfection kit (Takara Bio, USA) as described [20] ...