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Citations for Takara Bio :
1501 - 1550 of 1972 citations for 2 1H Imidazol 1 yl 6 methyl 4 pyrimidinemethanamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... EqPD-1 and EqPD-L1 cDNAs were amplified by PCR using TaKaRa Ex Taq (Takara Bio) and specific primers (Supplementary Table 1) ...
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bioRxiv - Neuroscience 2023Quote: ... Antibodies and dyes were used at following dilutions: rabbit-α-dsRed (1:500, Takara, #632496; RRID:AB_10013483), α-HRP conjugated with Alexa Fluor-488 (1:250 ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were added to the blocking solution (Rabbit anti-DsRed 1:1000, stock #632496, Takara Bio ...
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bioRxiv - Bioengineering 2023Quote: ... Cell and tissue were stained with the following primary antibody: rabbit anti-ZsGreen (1:500; Takara), rabbit anti-NeuN (1:500 ...
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bioRxiv - Molecular Biology 2023Quote: ... EGFP-SEC61B was generated by cloning the corresponding cDNA into the pEGFP.C1 vector (Clontech, 6084-1). The plasmid pRK5-HA-UB was described previously [56] ...
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bioRxiv - Physiology 2023Quote: ... Slides were incubated for 18 hours at room temperature in anti-dsRed antibody (TaKaRa, 1:2000) in 0.1M PB containing 0.3% Triton X-100 and 1% donkey serum ...
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bioRxiv - Biochemistry 2023Quote: ... the expression of GADD34Δ and K3L was induced by adding 1 µg/ml of doxycycline (Takara). After one additional day ...
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bioRxiv - Cell Biology 2023Quote: ... His-tagged recombinant proteins were isolated by 1 h incubation with Talon metal affinity resin (Clontech) at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... For immunostaining the slides were incubated with primary antibodies anti-DsRed (Takara Bio, #632496, 1/1000), anti-Pax7 (purified hybridoma ...
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bioRxiv - Cell Biology 2023Quote: ... Immunoblotting was performed using an anti-GFP mouse monoclonal antibody (JL8; 1:1000; TaKaRa Bio Inc.) and an anti-ubiquitin mouse monoclonal antibody (P4D1 ...
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bioRxiv - Neuroscience 2022Quote: ... then incubated overnight with the primary antibody rabbit anti-dsRed (1:1000 in PBST; Clontech, AB_10013483) at 4°C ...
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Constitutive and conditional epitope-tagging of endogenous G protein coupled receptors in DrosophilabioRxiv - Neuroscience 2023Quote: Myristoylated TdTomato (myr::TdTom) was labeled with 1:500 rabbit anti-DsRed (Takara Cat# 632496, RRID:AB_10013483)
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bioRxiv - Neuroscience 2024Quote: ... The following primary antibodies were used for staining: anti-FOXG1 (rabbit, Takara, M227, 1:400 dilution), anti-TCF7L2 (rabbit ...
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bioRxiv - Cell Biology 2023Quote: Procollagen Type 1 C-peptide (PIP) was measured according to the manufacturer’s instructions (Takara, Shiga, Japan). For evaluation of PIP ...
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bioRxiv - Neuroscience 2023Quote: ... a mix of an mCherry-DsRed rabbit polyclonal antibody (Living Colors-Clontech Antibody 632496, 1:1000) and a mouse monoclonal antibody against oxytocin (P38 ...
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bioRxiv - Plant Biology 2023Quote: ... 1 μg total RNA was reverse transcribed into cDNA with the SMARTer RACE 5’ Kit (Clontech). PCR was performed using primer GSP-HO1 and the Universal Primer Mix (UPM ...
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bioRxiv - Plant Biology 2024Quote: ... Both PHI-1 ORF and CESAs ORFs were amplified from first-strand DNA with PrimeScriptRTase (TaKaRa) using primers indicated in Supplemental information 4 ...
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bioRxiv - Plant Biology 2024Quote: ... 1 μg of total RNA was reverse transcribed using SMART® MMLV Reverse Transcriptase (Clontech, www.clontech.com) using oligo(dT)15 primers ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was directly loaded into a gravity column filled with 1 mL TALON resin (TaKaRa) pre-equilibrated with the binding buffer (20 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... Input and elution samples were analyzed by immunoblot using mouse anti-GFP (1:5,000, Clontech #632381) to detect A3-EGFP ...
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bioRxiv - Genetics 2022Quote: Full length wild-type or mutant BRC-1 sequences were cloned into plasmid pBridge (Takara Bio), transformed into yeast strain Y2HGold (Takara Bio ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 ng of total RNA was reverse transcribed with the cDNA synthesis kit (Takara Bio Inc.) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: Purified proteins or the artificial motor-cargo complex were diluted using 1× PBS (Takara Bio, T900) and loaded into custom-made microneedles prepared with the P1000IVF micropipette puller (Sutter Instrument) ...
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bioRxiv - Neuroscience 2024Quote: The primary antibodies used were: rabbit anti-dsRed (Living Colors® Clontech, used at 1/1000), rabbit anti-Vangl2 58 ...
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bioRxiv - Pathology 2024Quote: ... cDNA was synthesized from 1 µg of RNA with a PrimeScript RT reagent Kit (Takara, RR037A). Pluripotency genes were amplified with gene-specific primers by PCR using Taq DNA polymerase (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was synthesized from 1 µg of total RNA using the Primescript RT Master Mix (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... The following primary antibodies were used for staining: anti-FOXG1 (rabbit, Takara, M227, 1:400 dilution), anti-TCF7L2 (rabbit ...
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bioRxiv - Cell Biology 2024Quote: ... 1 µg of total RNA was reverse transcribed to cDNA using SMART MMLV reverse transcriptase (Takara) and Oligo(dT)20 as a primer ...
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bioRxiv - Plant Biology 2024Quote: ... was amplified from Tak-1 genomic DNA by PCR using PrimeSTAR Max (Takara Bio Inc., Japan) with the primers IF-MpRLFpro-F and IF-MpRLFpro-R ...
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bioRxiv - Plant Biology 2024Quote: ... we use 1 μg of purified total RNA using the PrimescriptTM RT master mix (Takara, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... 1 ng RNA was reverse-transcribed using the SMART-Seq v4 Low Input RNA kit (Takara). cDNA was quantified and qualified using Qubit (Invitrogen ...
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bioRxiv - Biophysics 2021Quote: ... which was digested with XhoI and BamHI restriction enzymes from the EYFP-actin vector (#6902-1, Clontech) into the mClover2-C1 vector (Addgene plasmid #54577 ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA synthesized from 1 μg total RNA using PrimeScript™ RT reagent Kit with gDNA Eraser (Takara). Real-time PCR was performed using TB Green® Premix Ex Taq™ II (Tli RNaseH Plus ...
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bioRxiv - Cell Biology 2022Quote: Single guide RNAs (sgRNAs) in the pX330 vector (1 μg) were mixed with EGFP (0.1 μg; Clontech) and co-transfected into MAP4K3 k.o ...
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bioRxiv - Genetics 2020Quote: MtDNA was amplified from 50 ng of total DNA with the primers (LongR_mtDNA_Fw and LongR_mtDNA_Rv, Table 1) using PrimeSTAR GXL DNA polymerase (TAKARA, Japan) and following PCR conditions ...
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bioRxiv - Synthetic Biology 2021Quote: ... Concentrated protein was bound to 0.5–1 mL of His60 nickel or HisTalon cobalt resin (Takara Bio) for 0.5–1 hr at 4°C while rotating in a 10-mL Pierce disposable column (Thermo Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... We then added 0.6 μL RT-mix [16.7 U μL−1 SMARTScribe Reverse Transcriptase (Takara Bio, 639538), 1.67 U μl−1 Recombinant RNase Inhibitor (Takara Bio ...
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bioRxiv - Bioengineering 2020Quote: ... which was digested with XhoI and BamHI restriction enzymes from the EYFP-actin vector (#6902-1, Clontech) into the mClover2-C1 vector ...
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bioRxiv - Biochemistry 2020Quote: ... full-length (1-1143) or C-term (620-1143) was inserted into pGBKT7 (Clontech, Mountain View, CA) using SmaI/SalI restrictions sites ...
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bioRxiv - Cell Biology 2021Quote: ... Fibroblast cultures at passage 3 were cryopreserved by suspending cells in CELLBANKER 1 (Takara Bio, Shiga, Japan), slowly cooled to -80 °C using a Mr ...
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bioRxiv - Microbiology 2020Quote: ... cDNAs from 1 μg DNase-treated purified RNA were obtained using PrimeScriptTM RT Master Mix (#RR036A, TAKARA) following the manufacturer’s instruction ...
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bioRxiv - Physiology 2021Quote: ... 1 μg RNA was used to synthesize first strand of cDNA using PrimeScriptTM RT-PCR Kit (TaKaRa). qPCR was conducted using PrimeScriptTMRT Master Mix (TaKaRa ...
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bioRxiv - Immunology 2022Quote: ... 1 μg of total RNA was used to synthesize cDNA with PrimeScript RT reagent kit (RR047A, Takara) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... pro-meprin α was activated by trypsin cleavage applying immobilized trypsin on magnetic beads (PT3957-1, Takara, buffer 30 mM Tris-HCl pH 7.4 ...
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bioRxiv - Microbiology 2022Quote: ... Membrane was immunoblotted for ≥ 3 h with primary monoclonal anti-GFP (1:5,000) antibodies (JL8, Clontech-Takara), then followed by immunoblotting for ≤ 1 h with secondary antibodies ...
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bioRxiv - Microbiology 2022Quote: ... Membrane was immunoblotted for ≥ 3 h with primary monoclonal anti-GFP (1:5,000) antibodies (JL8, Clontech-Takara), then followed by immunoblotting for ≤ 1 h with secondary antibodies ...
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bioRxiv - Developmental Biology 2022Quote: ... Complementary DNA (cDNA) was prepared using the 5X Prime Script RT Master Mix (Takara RR-036A-1) with 1 μg of total RNA ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were incubated with a diluted primary antibody: rabbit polyclonal anti-DsRed at 1:1000 dilution (Takara Biomedical Technology ...
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bioRxiv - Cell Biology 2022Quote: ... cells were treated with the appropriate chemicals at the following concentrations: 1 µg/ml doxycycline (Clontech, 631311), 2.5 µM MG132 (APExBIO ...
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bioRxiv - Neuroscience 2022Quote: ... brain sections were incubated with rabbit anti-DsRed antibody (1:1,000; #632496, Takara Bio., Mountain View, CA) at room temperature overnight ...