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Citations for Takara Bio :
1451 - 1500 of 5597 citations for Hydrogen Peroxide Fluorescent Detection Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: Mammalian expression constructs were mainly generated by Gibson assembly of PCR-amplified coding sequences (primers, Supp. Data File 2) into restriction-digested pC1 (CMV promoter, modified from pEGFP-C1, Clontech) or pCAG (CMV enhancer fused to chicken beta-actin promoter ...
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bioRxiv - Molecular Biology 2023Quote: ... The sequence spanning the I-SceI cleavage site in the SceGFP gene was amplified from 150 ng of genomic DNA using Advantage 2 Polymerase (Clontech) with primers dr-for (5’-CCCGCCACCTGCCCCATCTGCTA ...
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bioRxiv - Cell Biology 2023Quote: SseI gene was amplified from Salmonella typhimurium (STM) strain LT-2 strain using high fidelity DNA polymerase enzyme from TaKaRa and cloned in with N-terminal flag and HA tag in mammalian expression vector pcDNA3 flag HA Akt1 plasmid obtained from addgene(1477) ...
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bioRxiv - Biochemistry 2023Quote: ... Clarified lysate was then filtered through a 0.45 µm syringe filter and mixed with 2 mL of washed TALON® Metal Affinity Resin beads (Takara). The mixture was allowed to bind under nutation at 4℃ for 1 hr ...
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bioRxiv - Genomics 2023Quote: ... The transfected cells were cultured for 2 days and lentivirus were harvested and concentrated using the Lenti-X concentrator (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: The dCas9-KRAB-NLS-NLS sequence was amplified from the plasmid PB-TRE-dCas9-KRAB-MeCP2 (Tan, 2022) (addgene 122267) using PrimeSTAR Max 2 ×premix (Takara). A T7 promoter sequence was included in forward primer for in vitro transcription using the mMESSAGE mMACHINE T7 Ultra Transcription kit (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... The upstream and downstream regions adjacent to the binding site were amplified using Advantage 2 polymerase (Takara Bio, Shiga, Japan) from 3T3-L1 genomic DNA and cloned into the HR110-PA-1 vector (System Biosciences) ...
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bioRxiv - Microbiology 2024Quote: ... The plasmids were used to transform competent yeast cells using YeastMaker Yeast Transformation System 2 (Takara, Mountain View, CA94043, USA), and the binding was validated in synthetic dropout-Leu-Trp-His-Ade medium supplemented with X-α-gal ...
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bioRxiv - Molecular Biology 2024Quote: Plasmids encoding SARS-CoV-2 NSP1 variants were generated by exchanging the EGFP coding sequence of pEGFP-N1 plasmid (Clontech) for the corresponding nsp1 genes ...
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bioRxiv - Cell Biology 2021Quote: ... samples were prepared for library prepraration using the SMARTer® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian Kit for Sequencing (Takara Bio USA, Mountain View, CA). Total RNA was quantified and purity ratios determined for each sample using the NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... Using the cDNA synthesis kit (TaKaRa: Moloney Murine Leukemia Virus Version), cDNA was synthesized from extracted RNA ...
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bioRxiv - Biochemistry 2020Quote: ... Transfection was carried out with CalPhosTM Mammalian Transfection Kit (Takara Bio) at a ratio of pLL3.7:psPAX2:MD2G = 20:15:6 ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA was then prepared using the PrimeScript RT reagent kit (TaKaRa). QPCR reactions were performed according to the manufacturer’s instructions using SYBR® Premix Ex Taq kit (TaKaRa) ...
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bioRxiv - Molecular Biology 2020Quote: ... total RNAs were extracted using the RNAplus Kit (TaKaRa, Dalian, China) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... One-Step TB Green PrimeScript PLUS RT-PCR Kit (Takara Bio) was used under the following conditions ...
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bioRxiv - Microbiology 2021Quote: ... with the high-fidelity One Step RT-PCR Kit (Takara Bio). PCR products were gel purified and cloned into the pCR4-TOPO vector using the Zero Blunt Topo Cloning Kit (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... The amplicons were purified by DNA Amplification Clean Up Kit (Clontech). Amplicons were pooled in equimolar ratios prior to library preparation ...
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bioRxiv - Microbiology 2019Quote: ... the viral RNA was purified by NucleoSpin® RNA Kit (TAKARA). Recombinant INHis proteins (300 nM ...
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bioRxiv - Developmental Biology 2021Quote: ... Total RNA was extracted using NucleoSpin RNAII kit (Takara, Cat#740955.50). qRT-PCR was performed on 96-well optical reaction plates with one-step SYBR Green PCR master mix (Bio-Rad Laboratories ...
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bioRxiv - Developmental Biology 2021Quote: ... and the PrimeScript RT Reagent Kit with gDNA Eraser (TAKARA BIO). Real-time RT-PCR was performed using the THUNDERBIRD SYBR qPCT Mix (TOYOBO ...
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bioRxiv - Evolutionary Biology 2020Quote: The SMARTer Ultra Low Input RNA Kit for Sequencing v4 (Takara) was used to generate first strand cDNA from 2.5 ng UNM ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Reverse transcription was performed using a PrimeScriptTM RT reagent kit (Takara), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Sequencing libraries were constructed using ThruPLEX DNA-seq kit (Takara R400428). Sequencing was performed as described above ...
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bioRxiv - Genomics 2022Quote: ... This was followed by a Reverse transcription reaction (SmartScribe kit Clontech) for 1 hour at 42°C and 70°C for 15 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... Reverse transcriptase kit and PrimeSTAR Max DNA Polymerase were from TaKaRa. RiboLock RNase Inhibitor and RNase A (10 mg/mL ...
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bioRxiv - Molecular Biology 2020Quote: RACE assay was performed with SMARTer RACE 5’/3’ kit (Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA libraries were generated using the SMART-Seq Stranded Kit (Takara). This kit incorporates SMART® cDNA synthesis technology (28 ...
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bioRxiv - Molecular Biology 2021Quote: ... Viral titer was determined using the Lenti-X GoStix kit (Takara) following the manufacturer’s recommendations
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bioRxiv - Genomics 2020Quote: ... we used the Cellartis® Hepatocyte Differentiation Kit (Takara Bio Inc.) and the Cellartis® DE Differentiation Kit (Takara Bio Inc.) ...
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bioRxiv - Cancer Biology 2020Quote: ... and SMARTer Stranded Total RNA-Seq Kit - Pico Input (Clontech; 635005) and sequenced on the Illumina HiSeq 2500 sequencer (Illumina ...
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bioRxiv - Immunology 2019Quote: ... Complementary DNA was obtained using Reverse Transcription Kit (Takara, Kyoto, Japan) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... and reverse-transcribed with a PrimeScript™ cDNA Synthesis Kit (TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2020Quote: PrimeScript RT Reagent Kit with gDNA Eraser – Perfect Real Time (Takara) was used according to manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: cDNA was prepared using SMARTer Ultra Low RNA kit (Clontech Laboratories) for Illumina Sequencing following the manufacturer’s protocol ...
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Macrophage-specific NF-kappa B activation dynamics can segregate inflammatory bowel disease patientsbioRxiv - Immunology 2019Quote: ... using a Lenti-X™ Provirus Quantitation Kit (Clontech; Oxford, UK). For in vitro stimulation ...
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bioRxiv - Microbiology 2019Quote: ... The In-fusion HD Cloning Plus kit was purchased from Takara Bio USA ...
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bioRxiv - Microbiology 2019Quote: ... Reverse transcription was performed using PrimeScript RT reagent Kit (Takara, Japan). qPCR was performed using specific primers and the SYBR Green PCR master mix (Applied Biosystems ...
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bioRxiv - Genetics 2019Quote: ... using the In-fusion HD Cloning Kit (Clontech, Mountain View, CA). The primers used to amplify the Snail 3′-UTR were ...
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bioRxiv - Molecular Biology 2019Quote: ... and library preparation using the SMARTer stranded RNA-seq Kit (Clontech). DNA libraries were quantified with KAPA Library Quantification Kit for Illumina (Kapa Biosystems ...
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bioRxiv - Molecular Biology 2019Quote: ... with SMART RACE cDNA Amplification kit (Clontech, Mountain View, California, USA) from 1 µg total RNA ...
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bioRxiv - Physiology 2021Quote: ... Plasmid isolation was done using Nucleobond Xtra Midi Kit (Takara #740410.50) following the manufacture’s protocol ...
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bioRxiv - Genomics 2021Quote: ... such as for the SMARTer Stranded Total RNA Kit (Takara, 634862), SMART-Seq RNA Kit (v4 ...
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bioRxiv - Cancer Biology 2020Quote: ... The titer of virus was measured using P24 ELISA kit (Clontech).The virus was aliquoted and stored at −80°C.
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bioRxiv - Plant Biology 2020Quote: The assay was performed using the 5’-Full RACE kit (TaKaRa) according to the manufacturer’s instructions with modifications ...
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bioRxiv - Neuroscience 2020Quote: ... We prepared donor plasmids using the InFusion HD Kit (Clontech, 638910) and NucleoBond Xtra Midi EF Kit (Macherey-Nagel ...
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bioRxiv - Microbiology 2021Quote: ... First-strand cDNA was synthesized using the PrimeScript RT kit (TakaRa). A real time quantitative PCR was used to detect the presence of SARS-CoV-2 viruses by the primers (table 1).
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bioRxiv - Neuroscience 2020Quote: ... Viral titer was determined by AAVpro® Titration kit ver2 (TaKaRa).
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bioRxiv - Neuroscience 2020Quote: ... We prepared donor plasmids using the InFusion HD Kit (Clontech, 638910) and EndoFree Plasmid Maxi Kit (Qiagen ...
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bioRxiv - Bioengineering 2020Quote: ... 500 ng were reverse transcribed using PrimeScript RT-PCR Kit (Takara), and the qRT-PCR analysis was performed using LightCycler 480SYBER Green I Master Mix (Roche Diagnostics) ...
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bioRxiv - Biochemistry 2020Quote: ... Clontech In-Fusion® Cloning Kit (638909) was purchased from Takara. All enzymes and buffers used in cloning were purchased from New England Biolabs.