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Citations for Takara Bio :
101 - 150 of 486 citations for Zinc Finger Protein 50 ZNF50 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Osh6 requires Ist2 for localization to the ER-PM contacts and efficient phosphatidylserine transportbioRxiv - Cell Biology 2020Quote: For testing protein-protein interactions Matchmaker™ GAL4 Two-Hybrid System 3 was used according to the manufacturer’s instructions (Clontech). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... pH 7.4) and the protein contents of the cell lysate were quantified using a BCA Protein Assay Kit (TAKARA, T9300A) following the manufacturer’s protocol (Song et al. ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatants were collected and mixed with Laemmli buffer after measuring the protein concentration using a TaKaRa BCA Protein Assay Kit (TaKaRa). Samples were subjected to SDS-polyacrylamide gel electrophoresis and electrotransferred onto polyvinylidene difluoride membranes ...
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bioRxiv - Cancer Biology 2024Quote: ... Eukaryotic expression vectors encoding green fluorescent protein (GFP)-tagged proteins were generated by inserting PCR-amplified fragments into pd1-EGFP-N1 vector (6073-1, Clontech). Prokaryotic plasmids encoding GST-fusion proteins were constructed using pGEX-4T-1 bacterial expression vector (27-4580-01 ...
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bioRxiv - Genomics 2020Quote: ... CAS9 protein was purchased from Clontech (Cat # 632641). For injection ...
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bioRxiv - Biochemistry 2021Quote: ... Protein was purified using amylose-agarose resin (Clontech) and eluted in 10 mM maltose ...
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bioRxiv - Biochemistry 2020Quote: ... Protein concentration was determined by Bradford assay (TAKARA). Nickel affinity purification of 10His-SUMO1T95R conjugates was carried out with Ni-NTA agarose beads (Qiagen) ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were bound to TALON IMAC resin (Clontech) overnight at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... and protein purified using TALON resin (Takara Bio) using standard protocols in 50 mM phosphate buffer pH7.4 containing 300mM NaCl.
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bioRxiv - Molecular Biology 2022Quote: ... The protein was purified by TALON affinity (Clontech), HiTrap-Q anion-exchange (Cytiva ...
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bioRxiv - Microbiology 2022Quote: ... proteins were purified using Talon resin (Takara Bio) by resuspending the cell pellet in lysis buffer (50 mM phosphate buffer and 300 mM NaCl ...
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bioRxiv - Neuroscience 2023Quote: ... Red Fluorescent Protein Antibody (DsRed, 1:500, Clontech). The brain sections were incubated with antibodies in blocking solution at 4°C temperature overnight ...
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bioRxiv - Evolutionary Biology 2023Quote: ... recombinant protein (vector pColdTM TF-DNA, Takara Clontech) to use as the antigen ...
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bioRxiv - Evolutionary Biology 2023Quote: ... recombinant protein (vector pColdTM TF-DNA, Takara Clontech) to use as the antigen ...
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bioRxiv - Bioengineering 2023Quote: ... Using a BCA protein kit (TaKaRa, Shiga, Japan), a working solution (BCA reagent A ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were pre-hybridised at 50 °C in ExpressHyb Hybridization Solution (Clontech, USA) for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... Hybridisation was performed overnight at 50 °C in ExpressHyb Hybridisation Solution (Clontech, USA). After hybridisation ...
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bioRxiv - Bioengineering 2020Quote: ... and lentiviral vectors were concentrated 50-fold using Lenti-X concentrator solution (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... 50 ng of cDNA was used for quantification using SYBR Green mastermix (Takara) on Lightcycler 480 instrument (Roche) ...
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bioRxiv - Microbiology 2022Quote: ... and 50 ng mL−1 or 25 ng mL−1 anhydrotetracycline (aTc, Clontech) and Aeromonas sp ...
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bioRxiv - Synthetic Biology 2022Quote: ... KO primers with 50 bp homologous overhangs and PrimeStar GXL polymerase (Takara Bio) was performed to generate chloramphenicol (CapR ...
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bioRxiv - Biochemistry 2024Quote: ... After addition of DNaseI 1mM MgCl2 and 200μl 50% TALONR beads slurry (Clontech), the cell lysate was incubated at 10min at 4°C on a rolling shaker ...
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bioRxiv - Neuroscience 2022Quote: ... in PBS] for 90min followed by an incubation with a rabbit anti-DsRed antibody (1:2000, in blocking solution; Takara Bio-Living Colors, RRID #632496) overnight at 4°C ...
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bioRxiv - Genomics 2020Quote: ... The 50 µl PCR reaction contains 1.25 U PrimeSTAR GXL DNA Polymerase (Takara, R050), 1X PrimeSTAR GXL Buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... Plasmids were maxipreped by using NucleoBond Xtra EF kits (Takara Bio USA, 740424-50) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: All PCR reactions were performed in 50 μL reactions using ExTaq Polymerase (Takara Bio) with the following program:
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bioRxiv - Cell Biology 2022Quote: ... with or without 1.59 nM (50 μg/μL) bacteriophage λ DNA (48502 bp; TaKaRa). The ratio between condensin II and λ DNA was 1 condensin II ...
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bioRxiv - Cell Biology 2020Quote: ... reverse transcription was carried out using 50 U/well SmartScribe (SmS) RT enzyme (Takara) + 1x SmS First Strand buffer (Takara ...
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bioRxiv - Immunology 2020Quote: ... 50-100 ng of cDNA was used for quantification using SYBR Green mastermix (Takara) in Lightcycler 480 instrument (Roche) ...
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bioRxiv - Immunology 2023Quote: ... Probes (50 ng) were labeled with 32P using the Ladderman DNA labeling kit (TaKaRa) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: All PCRs were performed in 50 µL reactions using ExTaq Polymerase (Takara Bio #RR001B) with the following program:
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bioRxiv - Cell Biology 2021Quote: ... a plasmid expressing enhanced green fluorescent protein (EGFP) (Clontech), or in another series of experiments with pVSV-G ...
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bioRxiv - Biophysics 2020Quote: ... The protein was initially purified using Talon resin (Clontech) with a linear gradient of 50 to 200 mM imidazole ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were purified using TALON Metal Affinity Resin (Clontech) and dialyzed overnight against PBS buffer ...
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bioRxiv - Immunology 2020Quote: ... The protein concentration was determined by Bradford assay (Takara), and the cell lysate was mixed with 4x Laemmli loading buffer containing β mercapto-ethanol ...
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bioRxiv - Plant Biology 2022Quote: ... Bound proteins were detected using Hyper HRP Substrate (Takara). PtIns(3,5)P2 Grip protein (P-3516-3-EC ...
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bioRxiv - Biochemistry 2023Quote: ... The Bradford protein assay kit was purchased from Takara Bio (Shiga ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Proteins were bound to TALON SuperFlow IMAC resin (Takara) overnight ...
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bioRxiv - Microbiology 2024Quote: Protein concentrations were determined using the BCA method (TaKaRa BCA Protein Assay Kit ...
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bioRxiv - Plant Biology 2023Quote: ... These plasmids were transformed into the yeast strain AH109 for testing protein-protein interaction using the Matchmaker Gold system (Takara Bio, Kusatsu, Japan). Yeast transformation and growth were performed as described in [Pecher et al. ...
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bioRxiv - Neuroscience 2022Quote: ... slices were incubated in a blocking buffer containing the primary antibodies rabbit anti-mCherry (1:1500; cat. No. 632496, Takara Bio USA, Inc., Mountain View, CA, USA) and chicken anti-GFP (1:1500 ...
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bioRxiv - Immunology 2019Quote: ... non-tissue culture treated 24-well plates were coated with 50 μg/mL Retronectin (Takara) overnight at 4 °C and washed once with PBS ...
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bioRxiv - Biophysics 2020Quote: ... 50 µl of cell lysate was added to equal volume of LDH substrate (Takara, France) in a 96-well microplate and incubated at room temperature for 5 min ...
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bioRxiv - Microbiology 2019Quote: ... Alu-PCR was performed using 50 ng of gDNA and PrimeStar GXL DNA Polymerase (Takara) using the following conditions ...
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bioRxiv - Cell Biology 2023Quote: ... Contaminant DNA in 50 µg of extracted RNA was digested with DNaseI (cat. #2270A, Takara) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Equal amounts of protein were incubated with TALON beads (Clontech) pre-equilibrated with purification buffer at RT for 1.5 h with overhead rotation ...
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bioRxiv - Plant Biology 2020Quote: ... the proteins were purified by TALON Metal Affinity Resin (Clontech) and filtered with Amicon Ultra 0.5ml (30K ...
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bioRxiv - Synthetic Biology 2021Quote: ... concentrated proteins were determined those concentrations with Bradford (Takara Bio) or BCA (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... the YFP protein was detected using the GFP antibody (Clontech) at 1/5,000th and the UGPase antibody (Agrisera ...
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bioRxiv - Cell Biology 2020Quote: ... The solubilized protein was purified by Talon-resin (Clontech/Takara) using the hexa-histidine-tag fused at the N-terminus of the protein ...