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Citations for Takara Bio :
101 - 150 of 599 citations for Phthalic Acid Unlabeled 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Borr open reading frame corresponding to amino acids 113-221 was first amplified with a stop codon from LD36125 by PCR using PrimeStar (Takara) and cloned between AscI and NotI sites of pENTR (ThermoFisher ...
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bioRxiv - Immunology 2020Quote: ... For binding studies the 6xHis tag were removed from some Fabs by treatment with PreScission protease (MolBioTech; ThermoScientific) and the protein repurified on cobalt-nitrilotriacetic acid (Co-NTA) agarose (Clontech) followed by gel filtration chromatography on Superdex 200 (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... CPE 451 mCherry constructed by Gibson assembly by sub-cloning the 451 amino acids of CPE (without the Amphipathic Helix) into pmCherry-N1 (Clontech) vector using XhoI and BamHI restriction sites.As control vectors we used pEGFP-N3 and pmCherry-N1.All constructs were sequenced to confirm the fidelity of the process ...
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bioRxiv - Neuroscience 2023Quote: ... inserting an in-frame 15bp flexible linker sequence (encoding the amino acids GGGGA) followed by EGFP at the C-terminus using Infusion cloning (Clontech). For the Tg(NBT:ap2s1-gfp ...
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Molecular basis of proteolytic cleavage regulation by the extracellular matrix receptor dystroglycanbioRxiv - Biochemistry 2024Quote: ... A Flag tag x3 was inserted between amino acids 489 and 490 and a HA tag was inserted between amino acids 724 and 725 using In-Fusion Cloning Mix (Takara).
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-P-cadherin (TAKARA Cat#M127, 1:100 for IF); mouse anti-Rac1 (Abcam Cat#ab33186 ...
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bioRxiv - Immunology 2022Quote: ... BAC DNA was purified using the NucleoBond BAC 100 kit (Takara). Viral DNA was isolated from viral stocks by digestion with proteinase K (Roche ...
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bioRxiv - Developmental Biology 2019Quote: ... The BACs were purified with the nucleobond BAC 100 kit (Clontech). We co-injected 1 nl of 40 ng/μl Tol2 mRNA and 50–250 ng/μl of the cdh1:cdh1-sfGFP or cdh1:cdh1-TagRFP BAC transgene DNA into the lifting cell of the zygote of 0 to 20-minute old embryos ...
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bioRxiv - Genomics 2019Quote: ... Lentiviral particles were concentrated ∼100 times using Lenti-X Concentrator (Takara). LUHMES cells were seeded in a 12-well-plate format and immediately transduced with 30 ul of virus concentrate ...
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bioRxiv - Microbiology 2020Quote: ... Modified BACs were purified using a Nucleobond BAC 100 kit (Clontech). BAC quality was assessed by digestion with RsrII and SbfI (New England Biolabs) ...
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bioRxiv - Developmental Biology 2022Quote: ... The final BAC was purified with Nucleobond BAC 100 kit (Clontech) and co-injected with I-Sce I into one-cell-stage zebrafish embryos ...
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bioRxiv - Developmental Biology 2022Quote: ... The final BAC was purified with Nucleobond BAC 100 kit (Clontech) and co-injected with 50 ng/μl Tol2 RNA into one-cell-stage zebrafish embryos ...
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bioRxiv - Neuroscience 2022Quote: ... neurons were incubated with 100 nM linker (AP21967; Takara, Cat# 635056) for 2 h before fixation.
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bioRxiv - Synthetic Biology 2021Quote: ... which contains a six-amino acid His-tag for the creation of a N-terminal fusion using the In-Fusion HD cloning kit (Clontech, USA).
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bioRxiv - Molecular Biology 2021Quote: ... two to three amino acids in GFP-SP-BAP were replaced by alanine for each construct using the CloneAmp HiFi PCR Premix (Takara Bio) according to the manufacturer.
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bioRxiv - Neuroscience 2022Quote: ... Pak1 fragment (amino acids 270–521) was PCR-amplified from pCMV6M-Pak1 and subcloned into pCold-Pros2 vector (Takara Bio Inc.) to generate pCold-Pros2-Pak1cat ...
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bioRxiv - Immunology 2023Quote: ... The expression vectors for the S variants with multiple amino acid changes or deletions were generated using the In-Fusion HD cloning kit (Takara Bio). The pcDNA3.1-hACE2 used to express human ACE2 (hACE2 ...
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bioRxiv - Neuroscience 2023Quote: ... lysates were centrifuged at 31,000 x g for 45 min at 4°C and the supernatant was mixed with nickel-nitrilotriacetic acid (Ni-NTA) beads (Takara, 635653) at 4°C for 2 h ...
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bioRxiv - Molecular Biology 2023Quote: ... The solution was treated with 5 μg/mL RNaseA and 70 unit/ml DNase I (Takara) for 1 h at 37°C ...
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bioRxiv - Genomics 2019Quote: ... and 5 mg/mL Doxycycline (Clontech).
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bioRxiv - Cell Biology 2020Quote: ... 0.125 μg/mL aureobasidin A (Takara), 50 μM cerulenin (Cayman) ...
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bioRxiv - Cancer Biology 2019Quote: ... 1 µg/mL of puromycin (Clontech) was added to cells for 2 weeks ...
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bioRxiv - Cell Biology 2021Quote: ... and doxycycline (2 mg/ml, Clontech). After 6 hours ...
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bioRxiv - Cell Biology 2019Quote: ... 50 µg/mL doxycycline (Clontech # 631311), 40 nM Antimycin (Sigma-Aldrich # A8674) ...
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bioRxiv - Cell Biology 2021Quote: ... containing doxycycline (2 mg/ml, Clontech). We kept the cells in this medium for 5 days ...
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bioRxiv - Microbiology 2022Quote: ... 1 mL RNAiso Plus reagent (TakaRa) was added to each bacterial cell pellet and mixed vigorously by pipetting ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μg/ml of puromycin (Takara) was added to the medium only for two days.
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bioRxiv - Developmental Biology 2023Quote: ... 1 μg/ml Doxycycline (Dox) (Clontech) was added into PGC-cultured FAcs medium 24 hours before the injection ...
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bioRxiv - Genomics 2023Quote: ... and doxycycline (200 ng/ml; Clontech) on laminin-521 (Biolamina ...
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bioRxiv - Cancer Biology 2023Quote: ... 500 ng/mL doxycycline (Takara Bio) was added to the media and cells were incubated for 48 h at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... doxycycline (Dox) (Takara, 0.2 μg/ml), pan-caspase inhibitor Q-VD-Oph (QVD ...
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bioRxiv - Immunology 2024Quote: ... of 25 μg/mL Retronectin (Takara) in PBS and incubated at 4°C overnight ...
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bioRxiv - Cell Biology 2019Quote: ... Unincorporated UTPs were removed using CHROMA SPIN-100-DEPC-H2O columns (Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... Up to 100 cells/subset were collected in lysis buffer (Takara Clontech) containing RNAse inhibitors ...
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bioRxiv - Immunology 2019Quote: ... Up to 100 cells/subset were collected in lysis buffer (Takara Clontech) containing RNAse inhibitors ...
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bioRxiv - Genomics 2019Quote: ... We concentrated the virus 100-fold with Lenti-X concentrator (Clontech, 631231) following the manufacturer’s recommendations before aliquoting and freezing at -80C.
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bioRxiv - Molecular Biology 2019Quote: ... The modified BACs were purified using a Nucleobond BAC 100 kit (Clontech). BAC quality was assessed by digestion with RsrII and SbfI (New England Biolabs) ...
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bioRxiv - Microbiology 2019Quote: ... The modified BACs were purified using the NucleoBond BAC 100 kit (Clontech).
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bioRxiv - Cell Biology 2019Quote: ... Lentiviral supernatants were concentrated 100-fold before use (Lenti-X concentrator, Clontech). Concentrated virus was used for transducing primary fibroblasts and hTERT-RPE1 cells.
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bioRxiv - Molecular Biology 2019Quote: ... 100 nL lysis mix was added (0.8 U RNase inhibitor (Clontech, 2313A), 0.07% Igepal ...
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bioRxiv - Physiology 2020Quote: ... All conditions additionally contained 100 ng pDsRed2-ER (Takara Clontech, Kusatsu, Japan) as an endoplasmic reticulum (ER ...
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bioRxiv - Physiology 2020Quote: ... All conditions additionally contained 100 ng pDsRed2-ER (Takara Clontech, Kusatsu, Japan) as an endoplasmic reticulum (ER ...
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bioRxiv - Genomics 2019Quote: ... 10-U/μL SMARTScribe™ Reverse Transcriptase (100 U/μL; Clontech, 634936), and 3.2 μL of Polaris RT Plus Reagent (Fluidigm ...
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bioRxiv - Biophysics 2019Quote: ... D40A mutation and deletion of C-terminal 10 amino acids were introduced into pET15-sfGFP-minD by using the PrimeSTAR Max mutagenesis protocol (TaKaRa, Shiga, Japan). Similarly ...
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bioRxiv - Molecular Biology 2022Quote: ... Cleared lysate was bound to 3 mL (=1.5 mL bed volume) Talon SuperFlow Metal Affinity Resin (TaKaRa) per protein preparation ...
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bioRxiv - Cell Biology 2022Quote: ... Flt3 ligand (50 ng/ml) and IL-3 (20 ng/ml) onto plates coated with retronectin (Takara Bio).
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bioRxiv - Biophysics 2019Quote: ... The pCD86-EGFP had been created by inserting CD86 with a 21 amino acid linker coding region on its C-terminus immediately before EGFP in pEGFP-N1 (Clontech/Takara, Mountain View, CA). The CD86-21aa was amplified from pCD86-mEos2 (Mike Heilemann via Addgene ...
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bioRxiv - Cell Biology 2019Quote: Amino acid substitutions in RNF167 were made using site-directed mutagenesis by Prime Star GXL-DNA polymerase (R050A, Takara Bio Inc., Otsu, Japan) according to the manufacturer’s protocol with the following primers:
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bioRxiv - Genomics 2019Quote: ... 10 U/ml DNase I (TaKaRa 2270A) was added to the reaction after 3-min digestion and gently pipetting was repeated every 5-6 min digestion ...
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bioRxiv - Neuroscience 2022Quote: ... 40U/mL of RNAse inhibitor (Takara: 2313B)] ...