Labshake search
Citations for Takara Bio :
101 - 150 of 202 citations for H Leu arg pro gly nh2 2hcl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... and qPCRs were performed using TB Green Premix Ex Taq II (Tli RNase H Plus) (Takara) according to the manufacturer’s protocol.
-
bioRxiv - Cell Biology 2023Quote: ... His-tagged recombinant proteins were isolated by 1 h incubation with Talon metal affinity resin (Clontech) at room temperature ...
-
bioRxiv - Cancer Biology 2023Quote: ... in the presence of TB Green® Premix Ex Taq™ (Tli RNase H Plus) (Takara). qPCR amplification was carried out using an initial denaturation at 95°C for 10 sec ...
-
bioRxiv - Microbiology 2021Quote: ... RNase H-treated cDNA was used as a template for PCR with PrimeStar GXL Polymerase (Takara, Japan) and primers listed in Supplementary table 7 ...
-
bioRxiv - Microbiology 2022Quote: ... Membrane was immunoblotted for ≥ 3 h with primary monoclonal anti-GFP (1:5,000) antibodies (JL8, Clontech-Takara), then followed by immunoblotting for ≤ 1 h with secondary antibodies ...
-
bioRxiv - Microbiology 2022Quote: ... Membrane was immunoblotted for ≥ 3 h with primary monoclonal anti-GFP (1:5,000) antibodies (JL8, Clontech-Takara), then followed by immunoblotting for ≤ 1 h with secondary antibodies ...
-
bioRxiv - Cell Biology 2022Quote: ... The extract was incubated for 1 h at 4 °C with Talon resin (Clontech, Mountain View, CA), loaded onto a column ...
-
Pleiotropy in FOXC1-attributable phenotypes involves altered ciliation and cilia-dependent signalingbioRxiv - Genetics 2020Quote: ... qPCR reactions were run with SYBR® Premix Ex Taq (Tli RNAse H Plus) master mix (Clontech) on LightCycler® 96 Instrument and analysed using LightCycler® 96 Application (Roche Life Science) ...
-
bioRxiv - Biochemistry 2021Quote: ... After 12 h medium was collected and recombinant proteins were purified on TALON metal affinity resin (Takara). 200 μl of the resin was loaded onto 1 ml column (Bio-Rad ...
-
bioRxiv - Cancer Biology 2021Quote: ... and TB Green Premix Ex Taq II (Tli RNase H Plus) (Cat#: RR820A, TaKaRa Bio, Shiga, Japan). qPCR results were analyzed using LightCycler® 480 Software Version 1.5 (Roche Molecular Systems ...
-
bioRxiv - Cell Biology 2024Quote: Paraffin sections were used for H/E staining and ALP staining with a commercial staining kit (TaKaRa, TRACP & ALP double-stain kit ...
-
bioRxiv - Microbiology 2024Quote: ... qRT-PCR was performed using TB Green Premix Ex Taq™ II (Tli RNase H Plus) (Takara) and Step One Plus (Applied Biosystems ...
-
bioRxiv - Microbiology 2020Quote: ... Pseudovirus-containing supernatants were collected 48 h later and concentrated using Lenti-X™ concentrator kit (Takara, CA). Pseudovirus neutralization assay was then performed by incubation of SARS-CoV-2 pseudovirus with 5 folds serially diluted antibody with starting concentration of 1 μg/ml for 1 h at 37 °C ...
-
bioRxiv - Plant Biology 2022Quote: ... and histidine (H) and containing 5-Bromo-4-Chloro-3-Indolyl α-D-galactopyranoside (X-α-gal) (Clontech) to detect interactions ...
-
bioRxiv - Molecular Biology 2024Quote: ... Real time PCR was set up using TB Green Premix Ex Taq II (Tli RNase H Plus) (TakaRa) in a Quantstudio5 machine ...
-
bioRxiv - Immunology 2023Quote: ... RT-qPCR was performed using TB Green® Premix Ex Taq™ (Tli RNase H Plus) (Takara, RR420B) according to manufacturer’s instructions on an Applied biosystems StepOnePlus machine ...
-
bioRxiv - Immunology 2023Quote: Wt or mutated human (h)ALPK1 cDNA constructs were cloned into the pCMV-myc plasmid (Takara Bio Inc) and were all siRNA resistant against the hALPK1 siRNA (s37074 ...
-
bioRxiv - Cell Biology 2023Quote: ... and a PrimeScript RTreagent kit with gDNA Eraser and SYBR Premix Ex Taq II (TliRNase H Plus) (TaKaRa) and an ABI7500 qPCR instrument (Life Technologies) ...
-
bioRxiv - Bioengineering 2023Quote: ... qPCR was performed using TB Green® Premix Ex Taq™ II (Tli RNase H Plus) from TAKARA BIO USA INC (CA ...
-
bioRxiv - Molecular Biology 2024Quote: ... 5 µg RNase H-treated RNA was poly-A tailed with 2 U of poly(A) polymerase (Takara) for 1 hour at 37 ℃ ...
-
bioRxiv - Bioengineering 2024Quote: ... qPCR was performed using TB Green® Premix Ex Taq™ (Tli RNase H Plus) (#RR42WR, Takara Bio) with a final forward and reverse primer concentrations of 1 µM and cDNA concentrations of 2.5 ng/µl ...
-
bioRxiv - Molecular Biology 2020Quote: ... Cells were infected with concentrated lentivirus for 24 h in wells pre-coated with Retronectin (0.2 mg/ml; Takara) in expansion medium supplemented with protamine sulfate (10 μg/ml) ...
-
bioRxiv - Molecular Biology 2020Quote: ... at 50°C for 1hr followed by incubation with 0.5U/μL T4 RNase H at 37°C.The libraries were generated by PCR using LA Takara taq polymerase (Clontech) and oligos P5 and PE (see Table S1 ...
-
bioRxiv - Microbiology 2020Quote: ... The cDNA samples were subjected to real-time PCR with SYBR Premix Ex Taq Tli RNase H Plus (Takara) using an ABI 7500 Fast Real-Time PCR system (Applied Biosystems ...
-
bioRxiv - Microbiology 2020Quote: ... cDNA was subsequently used for real-time qPCR using TB Green Premix Ex Taq (Tli RNase H Plus) (Takara). Viral RNA from nasal washes was also used for whole viral genome sequencing ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Supernatant containing virus was collected 48 h later and 10-X concentrated using Lenti-X Concentrator (Takara Bio, Germany). AML-12 wild-type cells were transduced using concentrated virus and selected using 3 μg/mL of puromycin ...
-
bioRxiv - Microbiology 2024Quote: ... Lentiviral supernatant was harvested 48 h later and then filtered to be concentrated through a Lenti-X Concentrator (Takara) following manufacturer’s instructions ...
-
bioRxiv - Biophysics 2024Quote: ... to reduce the detergent concentration and incubated for 3 h with 10 mL Talon metal affinity resin (Takara Biotech) pre-equilibrated with buffer S supplemented with 0.1% β-DDM and 0.02% CHS ...
-
bioRxiv - Microbiology 2024Quote: ... Real time PCR analysis was done using TB Green Premix Ex Taq II (Tli RNase H Plus) from Takara in a CFX96 touch Real-Time PCR System (Bio-Rad ...
-
bioRxiv - Developmental Biology 2021Quote: ... Real-time quantitative PCR was performed in triplicate using the SYBR Premix Ex Taq (Tli RNase H Plus) kit (Takara). The expression of TBP was used for the normalization of mRNA expression ...
-
bioRxiv - Molecular Biology 2021Quote: ... Expression of these mRNAs was assayed using TB Green™ Premix Ex Taq™ II (Tli RNase H Plus; Takara) according to manufacturer’s instructions with QuantiTect primer assays (Qiagen) ...
-
bioRxiv - Genetics 2020Quote: ... qPCR was carried out using TB Green Premix Ex Taq (Tli RNase H Plus) (#RR420A, Takara Bio Inc., Kusatsu, Japan) and the Applied Biosystems StepOnePlus™ Real-Time PCR System (Thermo Fisher Scientific ...
-
bioRxiv - Genetics 2020Quote: ... qPCRs were carried out using TB Green Premix Ex Taq (Tli RNase H Plus) (#RR420A, Takara Bio Inc., Kusatsu, Japan) and the Applied Biosystems StepOnePlus™ Real-Time PCR System (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2021Quote: ... Following bulk BCR and TCR are prepared using SMARTer Mouse BCR IgG H/K/L Profiling Kit and SMARTer Mouse TCR a/b profiling kit separately (Takara). Based on the extracted mRNA amount of each sample ...
-
bioRxiv - Neuroscience 2020Quote: ... Media was collected 48 h after transfection and concentrated using Lenti-XTM concentrator (Takara Bio Europe SAS, cat. no. 631231) according to manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... The membranes were blocked in 5% non-fat milk in TBST for 1 h and immunoblotted with antibodies against GFP (anti-GFP.1, 4°C overnight, Takara Bio Clontech ...
-
bioRxiv - Cell Biology 2021Quote: ... The membranes were blocked in 5% non-fat milk in TBST for 1 h and immunoblotted with antibodies against GFP (anti-GFP.1, 4°C overnight, Takara Bio Clontech, 632381 or anti-GFP.2 ...
-
bioRxiv - Cell Biology 2022Quote: ... Lentiviral supernatants were collected 48 h post-transfection and concentrated by adding one volume of Lenti-X Concentrator (Takara Bio) to three volumes of lentivirus-containing supernatant and incubating at 4°C overnight ...
-
bioRxiv - Cancer Biology 2022Quote: ... cDNAs were diluted 1/10 and PCR reactions were conducted using SYBR qPCR Premix Ex Taq II Tli RNase H+ (TAKRR820W, Takara). Primers can be found in Supplementary Table 4 ...
-
X-ray mediated scintillation increases synaptic activity via Cerium-doped LSO and Channelrhodopsin-2bioRxiv - Neuroscience 2020Quote: ... 36-48 h post transfection lentivirus containing media was harvested and concentrated using Lenti-X concentrator (Takara, Mountain View, CA) as per manufacturer’s recommendations ...
-
bioRxiv - Immunology 2020Quote: ... The lentivirus-containing media was harvested 48 or 72 h after transfection and concentrated 40 – 50 times using Lenti-X concentrator (Takara Clontech). Sorted Treg cells were stimulated with plate-bound anti-CD3 (1 μg/ml ...
-
bioRxiv - Genomics 2020Quote: ... and quantitative real time PCR was performed following manufacturer’s instructions given in SYBR1 Premix ExTaq (TliRNAse H Plus) (Clontech,USA). The PCR amplification was carried out in Rotor-Gene-Q Real-Time PCR System (Qiagen ...
-
bioRxiv - Plant Biology 2020Quote: ... Membrane blocking was performed with 3%BSA in PBS-t buffer for 1 h at room temperature followed by incubation with Mouse-anti-GFP (TaKaRa) (1/5,000 ...
-
bioRxiv - Molecular Biology 2021Quote: ... Quantification of gene expression level was performed with TB Green™ Premix Ex Taq™ Tli RNase H Plus (Takara) on a LightCycler 480 system (Roche) ...
-
bioRxiv - Immunology 2020Quote: ... The lentivirus or Vpx-VLPs containing media was harvested 72 h after transfection and concentrated 80 times using Lenti-X concentrator (Takara Clontech) or Lenti Concentrator (Origene) ...
-
bioRxiv - Cell Biology 2022Quote: ... Deletion of the predicted helix motif in the hCAP-H N-tail was performed using PrimeSTAR Mutagenesis Basal Kit (TaKaRa). Primers used in the deletion were as follows ...
-
bioRxiv - Synthetic Biology 2022Quote: ... qPCR was conducted with 10ng genomic DNA in duplicate in a 20 μl reaction using the TB Green Premix Ex Taq II (Tli RNase H Plus, Takara) and the StepOnePlus™ Real-Time PCR System (ABI) ...
-
bioRxiv - Immunology 2022Quote: ... Culture supernatants containing lentivirus were collected 24 and 48 h after transfection and concentrated by a Lenti-X concentrator (Takara) followed by passing through a 0.45-μm PES filter ...
-
bioRxiv - Biochemistry 2024Quote: ... Mutations were introduced into the H-segment by site-directed mutagenesis with PrimeSTAR HS DNA Polymerase (Takara Bio, Shiga, Japan). Plasmids were purified using a ZymoPURE Midiprep Kit (Zymo Research ...
-
bioRxiv - Cell Biology 2023Quote: HeLa cells and hippocampal neurons were transfected with plasmids encoding FM4 recombinant receptors for 15 h and then treated with 2 μM DD-Solubilizer (TakaraBio/Clontech) to induce the release of the receptors from the ER into the secretory trafficking ...