Labshake search
Citations for Takara Bio :
1401 - 1450 of 5191 citations for Total Carbohydrate Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2022Quote: ... Mink ACE2 full length gene was amplified using long range PCR (LRPCR) amplification assay in two overlapping fragments using high-fidelity PrimeSTAR GXL DNA Polymerase (Takara Bio USA) as previously described (34,35) ...
-
bioRxiv - Plant Biology 2021Quote: ... The yeast strain AH109 was used for the yeast two-hybrid assay according to MatchmakerTM Two-Hybrid System 3 (Clontech, Palo Alto) and grown on YPD (full medium ...
-
bioRxiv - Plant Biology 2024Quote: The Y1H assays were conducted according to the manufacturer’s instructions (Matchmaker Gold Y1H Library Screening System; Clontech Laboratories, Mountain View, CA, USA). The pGADT7 empty vector with the pHis2 promoter and pGADT7-ZmHsf20 with the pHis2 empty vector were used as negative controls ...
-
bioRxiv - Cell Biology 2023Quote: ... The β-galactosidase activity was determined by ONPG (o-nitrophenyl β-D-galactopyranoside) liquid assay (quantitative) as mentioned elsewhere (Clontech Laboratories, 2008). β-galactosidase activity from each sample was calculated from at least three biological replicates and the concentration of cells (O.D.600 ...
-
bioRxiv - Bioengineering 2022Quote: HEK293T cells were harvested for RNA extraction using RNeasy Plus Mini Kit (Qiagene) or NucleoSpin RNA Plus Kit (Takara). RNA concentration was measured by nanodrop and 500ng to 1000ng total RNAs were used for reverse transcription in 10 ul reaction volume by High-Capacity RNA-to-cDNA kit (ThermoFisher) ...
-
bioRxiv - Neuroscience 2021Quote: ... Reverse transcription (RT) was then initiated with the cDNA synthesis kit (PrimeScript™ 1st strand cDNA Synthesis Kit; Takara) using the random hexamers option ...
-
bioRxiv - Microbiology 2021Quote: ... RNA was converted to cDNA with the PrimeScript™ RT reagent Kit Prime Script TMRT reagent Kit (Takara, Japan), and the cDNA was analyzed by qRT-PCR using TB Green® Premix Ex TaqTM II (Takara ...
-
bioRxiv - Microbiology 2020Quote: 5’-RACE analysis was performed using SMARTer RACE 5’/3’ kit and In-Fusion HD Cloning kit according to the manufacturer’s instructions with slight modifications (Clontech). 5’-RACE ready cDNA was synthesized from purified mRNA (TG ...
-
bioRxiv - Immunology 2021Quote: ... Cells were harvested after 3 d and AAV6 was purified with a filtration-based kit (Takara AAVpro Purification Kit) per the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2022Quote: ... The extracted probes were radioactively labeled with 2.5 μl [alpha-32P] dCTP (Amersham) according to the instructions of the kit (LaddermanTM Labeling Kit, Takara). The probe was mixed with 100 μl TE buffer (10mM ...
-
bioRxiv - Microbiology 2021Quote: ... Remaining DNA was removed using the TURBO DNA-free kit (Thermo-Scientific) and cDNA generated using the PrimeScript cDNA synthesis kit (Takara). qRT-PCR reactions were set up according to the manufacturer’s instructions (Alkali Scientific) ...
-
bioRxiv - Developmental Biology 2020Quote: ... Libraries were prepared using the SMART-Seq v3 Ultra Low RNA Input Kit for Sequencing and the Low Library Prep Kit v1 from Clontech. For the an3 RNAseq experiment ...
-
bioRxiv - Molecular Biology 2020Quote: ... the cDNAs were produced using BioRad Reverse Transcription Kit (iScriptTM Reverse Transcription Supermix) or the PrimeScript RT reagent kit (RR037A, TaKaRa). cDNAs from HEK and THP89 cells were quantified by quantitative PCR using Bio Rad’s SsoAdvanced Universal SYBR Green Supermix ...
-
bioRxiv - Immunology 2021Quote: ... Following bulk BCR and TCR are prepared using SMARTer Mouse BCR IgG H/K/L Profiling Kit and SMARTer Mouse TCR a/b profiling kit separately (Takara). Based on the extracted mRNA amount of each sample ...
-
bioRxiv - Neuroscience 2022Quote: ... RNA with RIN > 7 was prepared for sequencing using the RNAseq Ovation V2 kit (Nugen) or Smart-seq 4 low-abundance RNA kit (Takara) and sequenced on the Illumina HiSeq 4000 or Novaseq 2 platform in collaboration with Oxford Genomics Centre.
-
bioRxiv - Microbiology 2020Quote: ... Total viral DNA was extracted using Qiagen viral DNA extraction kit (QIAamp DNA Mini Kit, Hilden, Germany) and DNA polymerase Tag (Takara) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2019Quote: ... cDNA synthesis and library preparation was performed using the SMART-Seq v4 Ultra Low Input RNA Kit and Low Input Library Prep Kit v2 (Clontech), respectively ...
-
bioRxiv - Neuroscience 2019Quote: ... cDNA synthesis and library preparation was performed using the SMART-Seq v4 Ultra Low Input RNA Kit and Low Input Library Prep Kit v2 (Clontech), respectively ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... the manufacturer specifically states they should not occur in RNA-Seq reads if used in combination with the Nextera XT DNA library preparation kit (Appendix C in SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing User Manual, Takara Bio Inc ...
-
bioRxiv - Microbiology 2023Quote: ... Remaining DNA was removed using the TURBO DNA-free kit (Thermo-Scientific) and cDNA generated using the PrimeScript cDNA synthesis kit (Takara). qRT-PCR reactions were set up according to the manufacturer’s instructions (Alkali Scientific) ...
-
bioRxiv - Genomics 2023Quote: ... A total of 3.5 μl ribo-depleted material was used for SMARTer (SMARTer PCR cDNA Synthesis Kit, catalog num. 634926 and Advantage 2 PCR kit, catalog num. 639207, Clontech-Takara) protocol ...
-
bioRxiv - Neuroscience 2023Quote: ... Isolated RNA was dissolved in 10 μl of DEPC-treated water and reverse-transcribed using a reverse transcription reagent kit (PrimeScript RT Reagent Kit with gDNA Eraser, Takara) and a thermal cycler (Mastercycler ...
-
bioRxiv - Cancer Biology 2024Quote: ... The full-length cDNA of the human ZNRF3 was acquired from the human CRC DLD-1 cell line by RNA purification using NucleoSpin RNA II kit (Macherey Nagel) and reverse transcription RT-PCR using specific primers and Primescript RT reagent kit (TaKaRa). Then the ORF of the ZNRF3 short variant replaced RNF43 and was assembled into the C-terminal FLAG-tagged vector using Q5 Hot Start High-Fidelity DNA Polymerase (New England Biolabs ...
-
bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted using a NucleoSpin RNA kit (Macherey Nagel) and reverse transcribed with the Primescript RT reagent kit (TaKaRa) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2020Quote: ... Total normal liver RNAs were obtained from 5 donors pool (Biochain, Newark, CA) and from 4 donors pool (Takara BioUSA, Mountain View, CA, USA). 3 μg of total RNA were used for reverse transcription to obtain cDNA and real-time PCR performed ...
-
bioRxiv - Cancer Biology 2019Quote: ... 3 μg of total RNA was reverse transcribed to cDNA with Oligo(dT)15 primers using PrimeScript™ Reverse Transcriptase (Takara Bio, Shiga, Japan). The resultant cDNA was subjected to real-time PCR by using SYBR® Premix Ex Taq™ II (Takara Bio ...
-
bioRxiv - Bioengineering 2024Quote: ... To synthesize cDNA, total RNA (8 µL, ca. 500 ng) was mixed with 2 µL of PrimeScript™ RT Master Mix (Takara Bio, Kusatsu, Japan) and incubated at 37°C for 15 min ...
-
bioRxiv - Microbiology 2020Quote: For Bioluminescence Resonance Energy Transfer (BRET) assay we used the pEYFP-C1/N1 plasmids encompassing EYFP tag in the N- or C-terminal positions (Clontech, Mountain View, CA). To obtain pNluc-C1/N1 plasmids ...
-
bioRxiv - Developmental Biology 2021Quote: ... Extent of SRY-dependent expression of β-galactosidase was evaluated by quantitative assay of enzyme activity in liquid culture using ortho-nitrophenyl-β-galactoside (ONPG) as described by the vendor (Clontech Laboratories, Inc). Mammalian Cell Culture—Rodent preSertoli-cell line ...
-
bioRxiv - Neuroscience 2020Quote: ... The reaction was performed in a final volume of 12 μL using the Premix Ex Taq Probe based qPCR assay (Takara Biotechnology, ref. RR390A). All reactions were run in duplicate ...
-
bioRxiv - Microbiology 2020Quote: ... SYBRgreen real-time quantitative assays were made by using the SYBR® Premix Ex Taq™ II (Tli RNaseH Plus; Takara Bion Inc.) and an Applied Biosystems® Step One PCR System ...
-
bioRxiv - Plant Biology 2023Quote: ... selections and LacZ reporter assays were made according to the Matchmaker Gold Yeast Two-Hybrid System user manual (Clontech; Mountain View, Ca, USA).
-
bioRxiv - Neuroscience 2023Quote: ... The qPCR reaction was performed on 96-well plates in a final volume of 12 μL using the Premix Ex Taq Probe based qPCR assay (Takara Biotechnology, ref. RR390A). All reactions were run in duplicate on a StepOnePlus Real-Time PCR System (Applied Biosystems ...
-
bioRxiv - Physiology 2024Quote: Flies treated with the same procedure as the lifespan assay were collected after 7-day EF/Sham exposure and homogenized in RNAiso reagent (Takara Bio, Shiga, Japan). Ten to twenty flies were homogenized in one tube ...
-
bioRxiv - Cancer Biology 2021Quote: ... a Prime Script RT kit (Takara, Tokyo, Japan) was adopted for the generation of complementary DNA ...
-
bioRxiv - Systems Biology 2020Quote: Human mitochondrial to nuclear DNA ratio kit (Takara) was used to assess mitochondrial DNA content ...
-
bioRxiv - Neuroscience 2020Quote: We used the In-Fusion cloning kit (Takara) to amplify the cDNA of the long isoform of UNC-13 (UNC-13L ...
-
bioRxiv - Microbiology 2021Quote: ... ligated together using Mighty Mix ligation kit (Takara), cloned into competent DH5α E ...
-
bioRxiv - Developmental Biology 2020Quote: ... using the In-fusion HD cloning kit (Clontech) according to supplier’s instructions.
-
bioRxiv - Genetics 2022Quote: ... GenTLE (from yeast) high recovery kit (Takara Bio). The genomic DNA concentration was measured using the Quantifluor® ONE dsDNA Dye System (Promega ...
-
bioRxiv - Molecular Biology 2020Quote: ... and SMARTer DNA Unique Dual Index Kit (TAKARA) according to manufacturer instructions ...
-
bioRxiv - Molecular Biology 2020Quote: The SMART® cDNA Library Construction Kit (Clontech) was used to reverse transcribe the zebrafish heart RNA ...
-
bioRxiv - Molecular Biology 2021Quote: ... One-step PrimeScript miRNA cDNA Synthesis Kit (Takara) was applied for reverse transcription ...
-
bioRxiv - Molecular Biology 2020Quote: ... or PrimeSTAR GXL DNA polymerase kit (Takara Co.). The amplicons were subcloned into the TOPO™ vector (Invitrogen ...
-
bioRxiv - Genomics 2020Quote: ... and quantified using a Library Quantification Kit (Clontech), before sequencing on the Illumina HiSeq 1500 platform.
-
bioRxiv - Immunology 2021Quote: ... Libraries were prepared using smRNA-seq kit (TAKARA) with the input of 1 µg µRNA for all three samples with positive and negative controls ...
-
bioRxiv - Immunology 2021Quote: ... and the PrimeScriptTM RT reagent Kit (TaKaRa, Japan) as described in previous studies (Li et al. ...
-
bioRxiv - Molecular Biology 2021Quote: ... using In-Fusion HD Cloning Kit (Takara, 639649). Mutants for miR-320a sites were generated using site-directed mutagenesis by replacing the miR-320a seed match sequence with the HVS miR-HSUR4 seed match sequence ...
-
bioRxiv - Microbiology 2019Quote: ... In-Fusion HD Cloning Kit (TaKara Bio, USA) was used for fast ...
-
bioRxiv - Cell Biology 2019Quote: ... using the In-Fusion HD cloning kit (TaKaRa) according to manufacturers instructions ...