Labshake search
Citations for Takara Bio :
1351 - 1400 of 5307 citations for Mouse Putative Phospholipase B Like 2 PLBD2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... Fractionated CD34+ cells from Animals #2 and #3 were cultured overnight on RetroNectin-coated plates (Takara, T100B, Mountain View, CA) in X-VIVOTM 10 (Lonza ...
-
bioRxiv - Plant Biology 2024Quote: ... The genomic DNA sequences surrounding the potential off-target sites selected for further analysis (Table 2) were amplified by PCR using specific primers (Supplementary Table S1) and PrimeSTAR GXL DNA Polymerase (Takara) from CRISPR/Cas-edited hairy roots (R1 of E-CP1 [the main root and five lateral roots] ...
-
bioRxiv - Developmental Biology 2023Quote: The dCas9-KRAB-NLS-NLS sequence was amplified from the plasmid PB-TRE-dCas9-KRAB-MeCP2 (Tan, 2022) (addgene 122267) using PrimeSTAR Max 2 ×premix (Takara). A T7 promoter sequence was included in forward primer for in vitro transcription using the mMESSAGE mMACHINE T7 Ultra Transcription kit (Thermo Fisher Scientific) ...
-
bioRxiv - Cell Biology 2023Quote: HeLa cells and hippocampal neurons were transfected with plasmids encoding FM4 recombinant receptors for 15 h and then treated with 2 μM DD-Solubilizer (TakaraBio/Clontech) to induce the release of the receptors from the ER into the secretory trafficking ...
-
bioRxiv - Microbiology 2024Quote: Each individual SARS-CoV-2 fragment was amplified from their respective plasmids using an exclusive pair of primers (Supplementary Table 2) and high-fidelity PrimeSTAR GXL DNA polymerase (Takara), followed by gel isolation with NucleoSpin Gel and PCR Clean-up (Macherey-Nagel ...
-
bioRxiv - Immunology 2024Quote: ... T cells were activated for 2 days and NK cells were activated for 4 days followed by retronectin (Takara, T100B) mediated viral transduction ...
-
bioRxiv - Microbiology 2023Quote: ... Plasmids expressing HuSaV (GI.2) and PSaV protease-polymerase fusion were generated by cloning into the XhoI and BamHI sites of pmCherry-C1 (Clontech). Vectors expressing RIG-I-CARD domain [25] and IRF3-5D [26] were previously described ...
-
bioRxiv - Neuroscience 2023Quote: Mammalian expression constructs were mainly generated by Gibson assembly of PCR-amplified coding sequences (primers, Supp. Data File 2) into restriction-digested pC1 (CMV promoter, modified from pEGFP-C1, Clontech) or pCAG (CMV enhancer fused to chicken beta-actin promoter ...
-
bioRxiv - Cell Biology 2024Quote: iPSCs SFCi55 and hESCs RUNX1-GFP were plated at 3 × 105 cells per a well of a 6 well plate and reverse transfected with 2 μg of DNA using the Xfect Transfection reagent (Clontech) and analyzed 2 days later.
-
bioRxiv - Microbiology 2024Quote: ... individual FLAG-HA tagged Vpr genes were amplified from the pLRGatewayIRESeYFP-Vpr plasmids using the primers in Table 2 and subcloned into pLVX-TetOne-puro (Clontech) using EcoRI and BamHI restriction sites ...
-
bioRxiv - Microbiology 2024Quote: ... Y453F and T76G were inserted in plasmid F5 or F1 respectively by Directed Site Mutagenesis using the primers (Supplementary Table 2) and high-fidelity PrimeSTAR GXL DNA polymerase (Takara).
-
bioRxiv - Cell Biology 2021Quote: ... samples were prepared for library prepraration using the SMARTer® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian Kit for Sequencing (Takara Bio USA, Mountain View, CA). Total RNA was quantified and purity ratios determined for each sample using the NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific ...
-
bioRxiv - Plant Biology 2020Quote: ... Using the cDNA synthesis kit (TaKaRa: Moloney Murine Leukemia Virus Version), cDNA was synthesized from extracted RNA ...
-
bioRxiv - Biochemistry 2020Quote: ... Transfection was carried out with CalPhosTM Mammalian Transfection Kit (Takara Bio) at a ratio of pLL3.7:psPAX2:MD2G = 20:15:6 ...
-
bioRxiv - Cancer Biology 2021Quote: ... cDNA was then prepared using the PrimeScript RT reagent kit (TaKaRa). QPCR reactions were performed according to the manufacturer’s instructions using SYBR® Premix Ex Taq kit (TaKaRa) ...
-
bioRxiv - Molecular Biology 2020Quote: ... total RNAs were extracted using the RNAplus Kit (TaKaRa, Dalian, China) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... One-Step TB Green PrimeScript PLUS RT-PCR Kit (Takara Bio) was used under the following conditions ...
-
bioRxiv - Microbiology 2021Quote: ... with the high-fidelity One Step RT-PCR Kit (Takara Bio). PCR products were gel purified and cloned into the pCR4-TOPO vector using the Zero Blunt Topo Cloning Kit (Invitrogen) ...
-
bioRxiv - Microbiology 2020Quote: ... The amplicons were purified by DNA Amplification Clean Up Kit (Clontech). Amplicons were pooled in equimolar ratios prior to library preparation ...
-
bioRxiv - Microbiology 2019Quote: ... the viral RNA was purified by NucleoSpin® RNA Kit (TAKARA). Recombinant INHis proteins (300 nM ...
-
bioRxiv - Developmental Biology 2021Quote: ... Total RNA was extracted using NucleoSpin RNAII kit (Takara, Cat#740955.50). qRT-PCR was performed on 96-well optical reaction plates with one-step SYBR Green PCR master mix (Bio-Rad Laboratories ...
-
bioRxiv - Developmental Biology 2021Quote: ... and the PrimeScript RT Reagent Kit with gDNA Eraser (TAKARA BIO). Real-time RT-PCR was performed using the THUNDERBIRD SYBR qPCT Mix (TOYOBO ...
-
bioRxiv - Evolutionary Biology 2020Quote: The SMARTer Ultra Low Input RNA Kit for Sequencing v4 (Takara) was used to generate first strand cDNA from 2.5 ng UNM ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Reverse transcription was performed using a PrimeScriptTM RT reagent kit (Takara), according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2020Quote: ... Sequencing libraries were constructed using ThruPLEX DNA-seq kit (Takara R400428). Sequencing was performed as described above ...
-
bioRxiv - Genomics 2022Quote: ... This was followed by a Reverse transcription reaction (SmartScribe kit Clontech) for 1 hour at 42°C and 70°C for 15 minutes ...
-
bioRxiv - Molecular Biology 2020Quote: ... Reverse transcriptase kit and PrimeSTAR Max DNA Polymerase were from TaKaRa. RiboLock RNase Inhibitor and RNase A (10 mg/mL ...
-
bioRxiv - Molecular Biology 2020Quote: RACE assay was performed with SMARTer RACE 5’/3’ kit (Clontech) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... RNA libraries were generated using the SMART-Seq Stranded Kit (Takara). This kit incorporates SMART® cDNA synthesis technology (28 ...
-
bioRxiv - Molecular Biology 2021Quote: ... Viral titer was determined using the Lenti-X GoStix kit (Takara) following the manufacturer’s recommendations
-
bioRxiv - Genomics 2020Quote: ... we used the Cellartis® Hepatocyte Differentiation Kit (Takara Bio Inc.) and the Cellartis® DE Differentiation Kit (Takara Bio Inc.) ...
-
bioRxiv - Cancer Biology 2020Quote: ... and SMARTer Stranded Total RNA-Seq Kit - Pico Input (Clontech; 635005) and sequenced on the Illumina HiSeq 2500 sequencer (Illumina ...
-
bioRxiv - Immunology 2019Quote: ... Complementary DNA was obtained using Reverse Transcription Kit (Takara, Kyoto, Japan) according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2019Quote: ... and reverse-transcribed with a PrimeScript™ cDNA Synthesis Kit (TaKaRa) according to the manufacturer’s instructions ...
-
bioRxiv - Synthetic Biology 2020Quote: PrimeScript RT Reagent Kit with gDNA Eraser – Perfect Real Time (Takara) was used according to manufacturer’s instructions ...
-
bioRxiv - Immunology 2019Quote: cDNA was prepared using SMARTer Ultra Low RNA kit (Clontech Laboratories) for Illumina Sequencing following the manufacturer’s protocol ...
-
Macrophage-specific NF-kappa B activation dynamics can segregate inflammatory bowel disease patientsbioRxiv - Immunology 2019Quote: ... using a Lenti-X™ Provirus Quantitation Kit (Clontech; Oxford, UK). For in vitro stimulation ...
-
bioRxiv - Microbiology 2019Quote: ... The In-fusion HD Cloning Plus kit was purchased from Takara Bio USA ...
-
bioRxiv - Microbiology 2019Quote: ... Reverse transcription was performed using PrimeScript RT reagent Kit (Takara, Japan). qPCR was performed using specific primers and the SYBR Green PCR master mix (Applied Biosystems ...
-
bioRxiv - Genetics 2019Quote: ... using the In-fusion HD Cloning Kit (Clontech, Mountain View, CA). The primers used to amplify the Snail 3′-UTR were ...
-
bioRxiv - Molecular Biology 2019Quote: ... and library preparation using the SMARTer stranded RNA-seq Kit (Clontech). DNA libraries were quantified with KAPA Library Quantification Kit for Illumina (Kapa Biosystems ...
-
bioRxiv - Molecular Biology 2019Quote: ... with SMART RACE cDNA Amplification kit (Clontech, Mountain View, California, USA) from 1 µg total RNA ...
-
bioRxiv - Physiology 2021Quote: ... Plasmid isolation was done using Nucleobond Xtra Midi Kit (Takara #740410.50) following the manufacture’s protocol ...
-
bioRxiv - Genomics 2021Quote: ... such as for the SMARTer Stranded Total RNA Kit (Takara, 634862), SMART-Seq RNA Kit (v4 ...
-
bioRxiv - Plant Biology 2020Quote: The assay was performed using the 5’-Full RACE kit (TaKaRa) according to the manufacturer’s instructions with modifications ...
-
bioRxiv - Neuroscience 2020Quote: ... We prepared donor plasmids using the InFusion HD Kit (Clontech, 638910) and NucleoBond Xtra Midi EF Kit (Macherey-Nagel ...
-
bioRxiv - Microbiology 2021Quote: ... First-strand cDNA was synthesized using the PrimeScript RT kit (TakaRa). A real time quantitative PCR was used to detect the presence of SARS-CoV-2 viruses by the primers (table 1).
-
bioRxiv - Microbiology 2021Quote: ... LDH release was quantified using an LDH Cytotoxicity Detection Kit (Clontech) according to the manufacturer’s instructions and normalized to mock-infected cells.
-
bioRxiv - Neuroscience 2020Quote: ... Viral titer was determined by AAVpro® Titration kit ver2 (TaKaRa).
-
bioRxiv - Neuroscience 2020Quote: ... We prepared donor plasmids using the InFusion HD Kit (Clontech, 638910) and EndoFree Plasmid Maxi Kit (Qiagen ...