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Citations for Takara Bio :
1251 - 1300 of 6507 citations for Rat G Protein Coupled Bile Acid Receptor 1 GPBAR1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2020Quote: ... proteins in whole cell extract and immunoprecipitated fractions were digested with 2 mg/mL proteinase K (Takara Bio Inc., Kusatsu, Japan) at 42°C for 2 h ...
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bioRxiv - Developmental Biology 2022Quote: ... Co-IPs were carried out by incubating the samples with 30 μL of protein A agarose bead slurry for 4h at 4°C in a rotating wheel and with anti-mCherry (Takara 632496) of 1:1000 dilution ...
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bioRxiv - Plant Biology 2023Quote: ... His8-tagged soluble or insoluble CML recombinant proteins were purified at room temperature using Ni-NTA resin (His60, Takara Bio Inc.) using the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... Recombinant His-tagged protein was purified from cell-free extracts using immobilized metal affinity chromatography (IMAC using Talon resin; Takara Bio). The buffer used during the purification process was 20 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2024Quote: ... the U2OS 2-6-3 cells expressing degron-tagged LacI fusion proteins were incubated with 300 nM Shield1 ligand (Takara Bio) and 1 µg/mL doxycycline for 24 hours ...
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bioRxiv - Evolutionary Biology 2024Quote: ... adhaerens Mint PDZ-1/PDZ-2/P1BM protein sequences (as described in the results section) were cloned into the mammalian bicistronic expression vector pIRES2-DsRed2 (Clontech, USA) to express recombinant proteins bearing C-terminal myc epitope tags (Figure 6K) ...
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bioRxiv - Immunology 2023Quote: ... domain followed by an Avi-Tag(76) and a hexahistidine tag was cloned into a mammalian protein expression vector (pADD2) by In-Fusion (Takara Bio).
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bioRxiv - Plant Biology 2023Quote: The gene encoding the PP1c1-293 protein (herein simply referred to as PP1c) was cloned into the pOPIN-SC3 vector (OPPF) via In-Fusion Cloning (Takara Bio), which provides a cleavable N-terminal 6xHIS-SUMO tag for purification and solubility ...
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bioRxiv - Evolutionary Biology 2023Quote: ... HeLa cells were co-transfected to express each of EGFP-fused MK-D1 proteins with an endoplasmic reticulum (ER) localizing mCherry construct using the Xfect transfection reagent (Takara Bio). At 24 h post-transfection ...
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bioRxiv - Systems Biology 2021Quote: ... We prepared ligation-free ribosome profiling and total RNA-seq libraries from the clarified polysome lysates treated for 6 hours following the instructions provided with their respective kits (smarter-seq smRNA-seq kit, Takara-Clontech; NEBnext Ultra-Directional II) augmented with our previously-published ligation-free ribosome profiling protocol42 ...
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bioRxiv - Cell Biology 2020Quote: ... Evo M-MLV RT Kit with gDNA Clean for qPCR (Accurate Biotech, Changsha, Hunan, China) and Mir-X™ miRNA First-Strand Synthesis Kit (Takara, Dalian, Liaoning, China) were applied for the transcription of RNA into complementary DNA (cDNA) ...
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bioRxiv - Cell Biology 2024Quote: ... (1:1000 anti GAPDH) (1:1000 anti-TdTomato rabbit primary antibody #632496 from Takara).
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bioRxiv - Microbiology 2020Quote: ... and reverse transcribed using the PrimeScript RT reagent kit (TaKaRa). The GPC segment was amplified and sequenced as previously described (9) ...
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bioRxiv - Cancer Biology 2021Quote: ... with SYBR Premix EX Taq kit (Takara Biotechnology Co., Ltd.). Primer sequences for RRM2 ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was synthesized using the PrimeScript RT Reagent Kit (Takara) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was generated by PrimeScript RT Reagent Kit (Takara RR037A). Real-time PCR was performed on 15 ng of cDNA using SYBR Premix Ex Taq (Tli RNaseH Plus ...
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bioRxiv - Cell Biology 2020Quote: ... with SYBR Premix Ex Taq II Kit (Clontech Laboratories, USA) and primers presented in Supplemental Table 2 ...
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bioRxiv - Genetics 2021Quote: ... Illumina small RNA-Seq kits (Clontech, Mountain View, CA, USA) were then used to generate cDNA libraries ...
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bioRxiv - Developmental Biology 2021Quote: ... SMART-Seq v4 Ultra-Low Input RNA kits (TaKaRa Bio) were used to generated polyA selected libraries from total RNA ...
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bioRxiv - Neuroscience 2021Quote: ... using CalPhos™ Mammalian Transfection Kit (Takara Bio or Promega) on DIV7 ...
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bioRxiv - Developmental Biology 2020Quote: ... and young adult) using the PrimeScript RT Reagent Kit (Takara). RT-qPCR was performed in a StepOnePlus(tm ...
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bioRxiv - Neuroscience 2021Quote: ... The SMART-Seq v4 Ultra Low Input RNA Kit (Clontech) was used for library preparation ...
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bioRxiv - Genomics 2020Quote: ... and the Cellartis® DE Differentiation Kit (Takara Bio Inc.), respectively ...
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bioRxiv - Developmental Biology 2019Quote: ... cDNA was synthesized using the PrimeScript RT Reagent Kit (Takara). qPCR was conducted using SYBR Premix Ex Taq (Takara) ...
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bioRxiv - Genomics 2019Quote: ... and the recently released RiboGone Mammalian kit (Takara Bio/Clontech). The latter combines hybridization of rRNA and mtRNA baits with RNAse H digestion ...
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bioRxiv - Genomics 2019Quote: ... and the recently released RiboGone Mammalian kit (Takara Bio/Clontech). The latter combines hybridization of rRNA and mtRNA baits with RNAse H digestion ...
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bioRxiv - Cell Biology 2019Quote: ... digested by BamHI using In-fusion HD cloning kit (Clontech Laboratories ...
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bioRxiv - Genomics 2019Quote: ... enhancer assay vector using In-Fusion HD cloning kit (Takara) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ... In-Fusion cloning (In-Fusion HD Cloning Plus Kit, Clontech) was used to fuse the fragments with the linearized backbone ...
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bioRxiv - Genomics 2021Quote: ... PrimeScript High Fidelity RT-PCR Kit (Takara Bio, Japan, R022A), In-Fusion Snap Assembly Master Mix (Takara Bio ...
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bioRxiv - Developmental Biology 2021Quote: ... and converted to cDNA by PrimeScript Reverse Transcriptase Kit (Takara). The resulting cDNA was subjected to PCR or qPCR analysis using Phire Green Hot Start II PCR Master Mix (Promega ...
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bioRxiv - Genomics 2021Quote: ... and reverse transcribed using the PrimeScript RT Reagent Kit (Takara). qPCR was performed using primers listed in Table 1.
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bioRxiv - Synthetic Biology 2021Quote: ... BAC DNA was isolated using Nucleobond XtraBAC kit (Takara 740436.25) according to manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... cDNA libraries were obtained using SMARTseq v4 kit (Clontech, # 634888) and sequenced using HiSeq 2500 sequencer ...
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bioRxiv - Developmental Biology 2021Quote: ... linearised with NheI using the InFusion kit (638909, Takara Bio). Fertilised single-cell embryos were injected with 30 pg of plasmid DNA and 25 pg of in vitro transcribed Ac mRNA (using mMESSAGE mMACHINE SP6 Transcription Kit ...
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bioRxiv - Microbiology 2021Quote: The Clontech SMARTSeq v4 Ultra-Low Input RNA kit (Takara) was used with 1.7 ng of input RNA from each sample ...
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bioRxiv - Cell Biology 2021Quote: ... The cDNA was synthesized using Primescript RT reagent kit (TakaRa), and qPCR analysis was carried out (14 ...
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bioRxiv - Cell Biology 2021Quote: ... Truncations were made using the InFusion HD Cloning Kit (Takara). The same construct was cloned into a modified pET-Duet-1 vector that contained an HRV protease cleavable N terminal 6x His-tag ...
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bioRxiv - Cancer Biology 2020Quote: ... using the SMARTer™ ICELL8 3’ DE Kit (Takara Bio). Processing 8 samples at a time ...
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bioRxiv - Neuroscience 2020Quote: ... or with EGFP (Control) using a CalPhos Transfection Kit (Takara) at DIV10 and immunostained at DIV14 ...
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bioRxiv - Physiology 2021Quote: ... and gel extracted (Clontech Nucleospin Extract II Kit Cat# 636972). The linearized and purified pMLM313 fragment was used as template for in vitro transcription of Cas9 mRNA with mMESSAGE mMACHINE T7 ULTRA kit (Life Technologies)16 ...
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bioRxiv - Plant Biology 2021Quote: ... We used the SMARTerTM RACE cDNA Amplification Kit (634923, Clontech) to determine the 5’- and 3’–ends using WTK4-specific primers (Supplementary Table 12 ...
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bioRxiv - Cell Biology 2021Quote: ... for doxycycline-inducible expression using InFusion HD cloning kit (TaKaRa). Additional GGGS linker was introduced between the Cry2olig and caspase/kinase domains or Cry2olig and mCherry to reduce potential sterical interference between domains ...
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bioRxiv - Microbiology 2021Quote: ... using the In-Fusion HD cloning kit (Clontech, Kusatsu, Japan) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... in Lysis buffer from NucleoSpin RNA Plus Kit (Clontech, USA). RNA from the lung homogenate or macrophages was isolated using NucleoSpin RNA Plus Kit (Clontech ...
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bioRxiv - Molecular Biology 2021Quote: ... using In-Fusion HD Cloning Kit (Takara bio, Shiga, Japan). In the next step ...
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bioRxiv - Molecular Biology 2021Quote: ... The One-step PrimeScript miRNA cDNA Synthesis Kit (Takara, Japan) was utilized for reverse transcription ...
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bioRxiv - Microbiology 2021Quote: ... and circularized using the In-Fusion HD Cloning Kit (Takara), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... mRNA was prepared using the NucleoTrap mRNA Mini Kit (Clontech) from 500 ng of total RNA ...
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bioRxiv - Neuroscience 2021Quote: ... Using the PrimeScript II 1st Strand cDNA Synthesis Kit (Takara), we reverse-transcribed the extracted mRNA into cDNA ...