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Citations for Takara Bio :
1251 - 1300 of 1577 citations for Musashi 1 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... The full-length and truncate CDS of TaNAM-A1a (residues 1–220) were cloned into bait vector pGBKT7 (Clontech) respectively to generate bait plasmids BD-NAM-A1full and BD-NAM-A11-220 ...
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bioRxiv - Molecular Biology 2023Quote: ... Equal loading of the GFP tagged E2F1 proteins was determined by the GFP antibody (Clontech, lot. 1404005; 1:2000) the secondary antibody anti-rabbit HRP (Na934v ...
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bioRxiv - Microbiology 2023Quote: ... the nine pmW118 plasmid vectors were subjected to amplification of the cDNA fragments (F1-F9-10) of SARS-CoV-2 XBB.1 and XBB.1.5 by PrimeSTAR GXL DNA polymerase (Takara) with the primer sets24 ...
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bioRxiv - Biochemistry 2023Quote: ... cDNA was prepared from 1 µg of total RNA using the PrimeScript RT Reagent Kit with gDNA Eraser (Takara). Real-time polymerase chain reaction (real-time PCR ...
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bioRxiv - Plant Biology 2024Quote: ... Transformed yeast cells were grown under selective conditions in a minimal medium (46.7 g L-1 Minimal SD Agar Base [TaKaRa] ...
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bioRxiv - Biophysics 2024Quote: ... the solution of carboxylated beads was diluted into 400 µL of 1× Phosphate-Buffered Saline (PBS) (Takara Bio, T900) containing 100 mg/mL PLL-g-PEG (SuSoS ...
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bioRxiv - Biochemistry 2024Quote: ... Induction of the constructs was done by supplementing the medium with 0.5 µg ml-1 tetracycline (Tet) (Takara Bio).
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bioRxiv - Plant Biology 2024Quote: ... First-strand cDNA was synthesized from 1 µg of purified total RNA using PrimeScript RT Master Mix (TaKara, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... 0.5-1.0 μg total RNA was used for cDNA synthesis with 5x PrimeScript RT Master Mix (Takara, RR036A-1). Quantitative real-time PCR was carried out using PowerUp SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Plant Biology 2020Quote: ... An additional BbsI site in the pDONR backbone was eliminated by site-directed mutagenesis using primers noBbsI_F and noBbsI_R (Supplemental Table 1) followed by an In-Fusion reaction (Takara Bio USA). In order to yield the final binary vectors ...
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bioRxiv - Microbiology 2020Quote: ... CTCF BS1 and CTCF BS2 were mutated by site-directed PCR mutagenesis using the primers detailed in Table 1 and Prime Star Max (Takara) mutagenesis kit following the manufacturer’s protocols and confirmed by sequencing ...
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bioRxiv - Developmental Biology 2021Quote: Cells were lysed and RNA was reverse transcribed and amplified according to the SMARTer Ultra Low RNA Kit for Illumina Sequencing (version 1, Clontech). Libraries were prepared from cDNA using the NEBNext Ultra DNA library preparation for Illumina sequencing with indexed adaptors (New England Biolabs) ...
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bioRxiv - Developmental Biology 2021Quote: Two-hybrid screening and assays were performed as described in the Matchmaker™ Pretransformed Libraries User Manual (Takara PT3183-1) using a Drosophila Mate & Plate™ Library - Universal Drosophila (Normalized A+ RNA isolated from embryo (~20 hr) ...
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bioRxiv - Genetics 2021Quote: ... Table 1) against two unique sequences in the spCas9 that differs from dCas9GCN4 using Xfect RNA transfection reagent (Takara #631450) according to manufacturer instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... DNA libraries were prepared using 1-5 ng of starting material using the SMARTer ThruPLEX DNA-Seq kit (Takara; R400674) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were diluted 30 or 60 times in 100 µl mTeSR with 1:1000 Y-27632 (STEMCELL, 72302) and 2µg/ml doxycycline (Clontech, 631311) and the cell suspension was added to a well containing the transfection complexes ...
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bioRxiv - Developmental Biology 2021Quote: The cDNAs for HA-tagged ELF3 were amplified from the KhES-1 cDNA library by PCR using PrimeSTAR GXL (Takara) and were subcloned into the pENTR/D entry vector (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... and first-strand cDNA was synthesized with 1 μg of total RNA using a SuperScript III First-Strand Synthesis System for RT-PCR (TaKaRa) following the manufacturer’s protocol.
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bioRxiv - Plant Biology 2022Quote: ... these plasmids were co-transformed into the Y2H GOLD strain in an activation domain (AD)-BD coupled manner (Yeast Protocols Handbook, PT3024-1; Clontech). The primers used are listed in Supplemental Table S1.
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bioRxiv - Neuroscience 2021Quote: ... Groups of 6-10 cells were dispensed from the micropipette into 1 µl ice-cold 10x reaction buffer (SMART-Seq v4 kit, Takara) and flash-frozen before library preparation ...
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miR-125-chinmo pathway regulates dietary restriction dependent enhancement of lifespan in DrosophilabioRxiv - Molecular Biology 2020Quote: ... The synthesized cDNA was diluted (1:10) and used as template for quantitative real-time PCR (qRT-PCR) using SYBR premix EX-Taq-plus (TaKara) and analyzed on QuantStudio 6 Real-Time PCR machine (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... The viral media was filtered through 0.45 μm filter followed by concentration through Amicon column and addition of 1/3rd volume of LentiX Concentrator (Takara #631231). Viral pellet was obtained by spin down at 1500g at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... coli MS115-1 (CD-NTase018 from (Whiteley et al., 2019)) were synthesized (GeneArt) and inserted into vectors pBridge and pGADT7 (Clontech) by isothermal assembly ...
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bioRxiv - Microbiology 2019Quote: ... the NS3 fragments with FLAG-tag and NS3 cDNA vector were ligated together at an approximate molar ratio of 1:3 using TaKaRa DNA Ligation Kit LONG (TAKARA) according to the manufacturer’s instructions.
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bioRxiv - Genomics 2019Quote: ... We spun down the aliquots and re-suspended the pellets in 1 ml DroNc-seq resuspension buffer (1X PBS, 0.01% BSA, 0.16 U/μl RNase Inhibitor (Clontech/TaKaRa, #2313A)) for Tube A and 1 ml 10X Nuclei resuspension buffer (1X PBS ...
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bioRxiv - Cancer Biology 2019Quote: ... Lentiviral particles were concentrated from supernatant by mixing 3 parts supernatant with 1 part Lenti-X concentrator solution (ClonTech 631231), incubating overnight at 4 °C ...
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bioRxiv - Microbiology 2019Quote: ... A plasmid encoding a gene fusion between the 5‘-201 nucleotides of PR8 segment 5 and GFP was made by PCR-cloning the appropriate IAV sequence into pEGFP-1 (Clontech), followed by oligonucleotide-directed PCR mutagenesis (using standard protocols ...
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bioRxiv - Cancer Biology 2020Quote: ... Total RNAs of 0.5 to 1 μg were used as templates for the reverse transcription using PrimeScriptTM RT Reagent Kit with gDNA Eraser (Takara). Quantitative PCR (qPCR ...
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bioRxiv - Cancer Biology 2019Quote: ... transfected cells were treated with 200 µg/mL hygromycin B (Nacalai Tesque, Kyoto, Japan) or 1 µg/mL puromycin (Clontech) for the selection of stably transfected cells.
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bioRxiv - Plant Biology 2021Quote: ... Membranes were blocked with 5% milk overnight at 4°C or 1h at room temperature for Anti-GFP JL-8 (1:4000; Clontech, California ...
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bioRxiv - Cell Biology 2021Quote: ... pLL5.0-EGFP-HRasC20 was generated by insertion of EGFP-HRasC20 fragment which was amplified from pEGFP-F (#6074-1; Clontech) by PCR into pLL5.0 vector ...
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bioRxiv - Neuroscience 2021Quote: ... Virus was then concentrated from the media 1:10 in PBS using Lenti-X concentrator (Takara Bio, Cat. No. 631231), aliquoted and stored at −80°C for future use.
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bioRxiv - Microbiology 2020Quote: ... Double stranded cDNA was prepared from 1 μg of each mRNA sample using the PrimeScript Double Strand cDNA Synthesis Kit (Takara) and a cDNA library was established with Ligation Sequencing Kit (SQK-LSK109 ...
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bioRxiv - Neuroscience 2020Quote: ... and first-strand cDNA was synthesized from 1 ⎧g of total RNA by oligo(dT) priming using SMARTScribe Reverse Transcriptase (Clontech) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2021Quote: ... The RNA (1 ug/sample) was reverse transcribed into cDNA and Q-PCR was performed using a SYBR Green PCR kit (Takara) in a Light Cycler (Eppendorf) ...
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bioRxiv - Systems Biology 2021Quote: ... cDNA was synthesized from 1 µg of total RNA using the PrimeScript RT reagent kit (Perfect Real Time) (cat. no. RR037A, TaKaRa) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... was co-transformed with different bait-prey combinations as indicated in Extended Data Fig.1 in accordance with instructions for Matchmaker GAL4 Two-Hybrid System 3 (Clontech). The transformed yeast was then screened in 4-dropout plates for the protein-protein interaction.
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bioRxiv - Microbiology 2019Quote: ... The resulting product was purified from a 1% agarose gel using the NucleoSpin Gel and PCR Clean-up Kit (Takara) and Sanger sequenced.
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bioRxiv - Cell Biology 2021Quote: ... following patch-clamp cells were collected using a separate wide-bore collection pipette (0.2-0.5 MOhm) filled with lysis buffer (10% Triton, ribonuclease inhibitor 1:40; Clontech, Cat#2313A), ERCC RNA spike-in mix (1:600000 ...
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bioRxiv - Cell Biology 2020Quote: ... GFP CIZ1 C275 was made by ligating the 1 kb C-terminal XhoI fragment (Coverley et al., 2005) into the XhoI site of pEGFP-C2 (Clontech). GFP CIZ1 Δp8 (this study ...
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bioRxiv - Cancer Biology 2020Quote: ... a targeting vector was constructed (Supplementary Figure 1) to include a heptameric tetracycline-response element (TRE) derived from the pTRE2 vector (Clontech), inserted into the intron 2 of the endogenous Myc locus that serves as a binding site for tetracycline-regulatable transcriptional repressors ...
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bioRxiv - Molecular Biology 2022Quote: ... gDNA extracted and PCR genotyped with primers (Supplemental table 1) flanking each homologous arms using PrimeStar GXL DNA polymerase (Takara). Correctly targeted clones were further expanded and banked ...
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bioRxiv - Microbiology 2022Quote: ... and A549-MxB(1-715)-MxB(26-715) cells expressing untagged MxB(1-715) and MxB(26-715) using the pLKOD-Ires-Puro vector (Clontech Takara Bio ...
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bioRxiv - Immunology 2022Quote: ... First-strand cDNA was generated from 1 μg of RNA for each sample using the SMARTer RACE 5’/3’ Kit (Takara Bio ...
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bioRxiv - Cancer Biology 2022Quote: ... cDNAs were diluted 1/10 and PCR reactions were conducted using SYBR qPCR Premix Ex Taq II Tli RNase H+ (TAKRR820W, Takara). Primers can be found in Supplementary Table 4 ...
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KIF24 controls the clustering of supernumerary centrosomes in pancreatic ductal adenocarcinoma cellsbioRxiv - Cell Biology 2022Quote: ... human KIF24 fragments encoding residue 1-4383 was excised from pEGFP-C1-KIF24 (Kobayashi et al., 2011) and sub-cloned into pLVX-IRES-Puro (Clontech). KIF24/TS621-622AA (TS ...
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bioRxiv - Neuroscience 2021Quote: ... We washed nuclei once with EZ PREP buffer and once with Nuclei Suspension Buffer (NSB; consisting of 1× PBS, 0.01% BSA and 0.1% RNase inhibitor (Clontech, Cat# 2313A)) ...
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bioRxiv - Neuroscience 2020Quote: ... cultures were infected with CC1-mycDD-expressing lentivirus at DIV4 and treated on DIV7 with 1 μM Shld1 (Takara Bio) or vehicle (0.2% (v/v ...
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bioRxiv - Molecular Biology 2021Quote: ... Quantitative real-time PCR reactions were performed using 1/60 of the immunoprecipitated DNA and the TB Green Premix Ex Taq II (Takara). Relative quantification using standard curve method was performed for each primer pair and 96-well Optical Reaction plates were read in a StepOnePlus PCR instrument (Applied Biosystem) ...
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bioRxiv - Molecular Biology 2020Quote: Primary RT-PCR and secondary PCR was performed using the Primescript III 1-step RT-PCR kit and EmeraldAmp GT PCR Mix (Takara; detailed protocol provided in Supplementary File 1) ...