Labshake search
Citations for Takara Bio :
1201 - 1250 of 2056 citations for Killer Cell Immunoglobulin Like Receptor 2DL3 KIR2DL3 Antibody PE since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted by suspending cells in RNAiso Plus reagent (Takara, 9108) and isolating RNA using chloroform as per the manufacturer’s protocol ...
-
bioRxiv - Bioengineering 2024Quote: ... coli and subsequently transformed into Brevibacillus choshinensis HPD31-SP3 competent cells (Takara, Japan) for protein expression following the protocol as recommended by the manufacturer ...
-
bioRxiv - Cancer Biology 2024Quote: Fluorescent constructs were introduced into TC71 cells using the pLVX lentiviral system (Clontech) and selected using antibiotic resistance to either puromycin or neomycin ...
-
bioRxiv - Cell Biology 2024Quote: ... were transfected into HEK293T cells using Xfect reagent (Clontech, Mountain View, CA, USA). After 48 hours of transfection ...
-
bioRxiv - Cell Biology 2024Quote: ... The GFP-LifeAct-expressing vector was transfected into Ampho-Pack 293 cells (Clontech) using Avalanche Everyday Transfection Reagent (EZ Biosystems ...
-
bioRxiv - Bioengineering 2024Quote: ... cells were treated with the ecotropic booster according to protocol (Takara Bio, 631471) before the supernatant of one 6-well production was applied to three wells ...
-
bioRxiv - Cell Biology 2024Quote: ... cells were subjected to plasmid transfection using a CalPhosT Mammalian Transfection Kit (Takara) according to the manufacturer’s instruction for 8∼16 h ...
-
bioRxiv - Cell Biology 2024Quote: ... we utilized the SMART-Seq Single Cell PLUS kit (96 reactions, Takara Bio). Single iMSC-L were sorted into 96 well plates containing 12.5 μl of CDS sorting solution provided in the kit ...
-
bioRxiv - Cell Biology 2024Quote: HEK 293T Lenti-X human embryonic kidney cells (Clontech, Mountain View, CA, USA), used to VLPs production ...
-
bioRxiv - Neuroscience 2021Quote: ... floating coronal sections were rinsed in PBS and blocked for 1–2 hr at room temperature in a solution of 10% normal goat serum and 0.5% Triton X-100 dissolved in PBS and then incubated in blocking solution containing rabbit anti-DsRed polyclonal antibody (1:1000; Takara Bio, Mountain View, CA) with gentle agitation at 4°C for 18–22 hr ...
-
bioRxiv - Synthetic Biology 2020Quote: ... dry milk and 0.1 % (v/v) Tween 20 and then incubated with mouse anti-6xHis tag monoclonal antibody-HRP conjugate (Clontech or Thermo Fisher Scientific, USA) in the same buffer for 2 h at room temperature ...
-
bioRxiv - Immunology 2020Quote: ... Then subsequent primary antibody incubation was done overnight at 4 degrees (all primary antibodies were diluted in 1% blocking milk or for Phos-tag with MBL Max blot solution 1 or Takara Western blot immune booster). Proteins of interest were detected with Anti-Rabbit IgG HRP (Cell signaling technologies ...
-
bioRxiv - Neuroscience 2023Quote: ... sagittal sections (50 µm) of the cerebellum were cut and incubated with antibodies against the reporter protein mCherry (Clontech 632543, RRID: AB_2307319, 1:500) and against the P-cell marker calbindin (Swant CB38 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Membranes were blocked as above and then incubated overnight at 4 °C with appropriate antibodies to GFP (1:1000 dilution; Clontech, Catalog No. 1. 632381) and tubulin α (1:2000 dilution ...
-
bioRxiv - Neuroscience 2023Quote: ... Overnight primary antibody incubation was done at 4°C with one or more of the following antibodies in blocking buffer: rabbit anti-DsRed (Takara Bio, 632496, 1:500), goat anti-mCherry (Sicgen ...
-
bioRxiv - Neuroscience 2024Quote: ... primary antibody staining (ALDH1L1, Abcam #ab871170 1:250; S100B, Synaptic Systems #287006 1:250; GFP, Abcam #ab13970 1:1000; Stem121, Takara #Y40410 1:500) was performed in staining buffer (2% normal horse serum ...
-
bioRxiv - Cell Biology 2020Quote: The commercially available Cellartis Human iPS Cell Line 12 (Takara Bio Inc., Kusatsu, Japan) was used as the human iPS Cell line ...
-
bioRxiv - Molecular Biology 2021Quote: Total RNA was extracted from the transfected cell lines using Trizol reagent (Takara, Japan). One-step PrimeScript miRNA cDNA Synthesis Kit (Takara ...
-
bioRxiv - Molecular Biology 2021Quote: ... NIH-3T3 cells (gift from Bart Vanhaesebroeck) and Lenti-X 293T (Takara Bio Europe) were cultured in high glucose DMEM (Thermofisher ...
-
bioRxiv - Genomics 2021Quote: ... Cells were infected to stably express LY6S-pQCXIP retroviral vector (Clontech Laboratories, Inc., USA) as described previously (45).
-
bioRxiv - Synthetic Biology 2021Quote: ... Dynabeads were removed and stimulated T cells were seeded on Retronectin (Takara Bio, Japan) coated nontissue culture treated plate with virus ...
-
bioRxiv - Biochemistry 2020Quote: ... the cells were harvested for analysis using Guide-it™ sgRNA Screening Kit (TaKaRa). Stable I2PP2A knockdown CRISPR I2PP2A HEK293 cells were also maintained in MEM with 10% FBS supplemented with Gentamycin (50 mg/ml).
-
bioRxiv - Cancer Biology 2021Quote: ... NSCLC cell lines were transfected by the mitochondrion-targeted DsRed (mtDsRed, Clontech, California, USA) plasmid or the enhanced green fluorescent protein (EGFP ...
-
bioRxiv - Cancer Biology 2020Quote: ... the lentiviral pCSIIneo or pCSIIbsr vectors were transfected into Lenti-X 293T cells (Clontech) together with the packaging plasmid psPAX2 (a gift from Dr ...
-
bioRxiv - Microbiology 2020Quote: RNA was extracted from Vero cells using the NucleoSpin RNA kit (Takara Bio Inc.), and cDNA was synthesized with the PrimeScript 1st strand cDNA Synthesis Kit (Takara Bio Inc.) ...
-
bioRxiv - Immunology 2021Quote: ... Target cell death was quantified with an LDH (lactate dehydrogenase) cytotoxicity assay kit (Clontech) using the manufacturer’s recommended protocol ...
-
bioRxiv - Cancer Biology 2020Quote: ... To encapsulate the construct for transduction we co-transfected Lenti-X 293T cells (ClonTech) with 10µg CENPA-FLAG-HA pLVX-TetOne and packaging vectors 7.5µg psPAX2 and 2.5µg pMD2.G using PolyPlus JetPrime transfection reagent according to manufacturer instructions ...
-
bioRxiv - Cancer Biology 2020Quote: ... 10 μl premix WST-1 cell proliferation reagent (Takara Bio Inc, Clontech Laboratories, Inc.) was added to each well ...
-
bioRxiv - Cell Biology 2020Quote: ... 293FT cells were transfected with the pLV constructs along with packaging plasmids pVSVG (Clontech) and pLV-CMV-delta 8.2 (Massey ...
-
bioRxiv - Immunology 2021Quote: ... Retroviral transduction of primary T cells was performed with retronectin-coated plates (Takara Bioscience). Naïve T cells had been activated as described in ‘Cell Cultures’ methods for two days before transduction ...
-
bioRxiv - Cancer Biology 2022Quote: ... from cells and reversed transcribed into cDNA using PrimeScript RT Master Mix (TaKaRa, Japan). qPCR was performed to detect mRNA expression of genes by TB Green PCR Kit (TaKaRa ...
-
bioRxiv - Microbiology 2022Quote: ... total RNA was isolated from cells using Trizol RNA isolation kit (Takara, Shiga, Japan) and MDR1 and GAPDH transcripts were detected using a TransScript Green One-Step qRT-PCR SuperMix (TransGen Biotech ...
-
bioRxiv - Microbiology 2022Quote: Doxycycline-inducible stable cells overexpressing Trim69 were generated using the pRetroX-Tight system (Clontech), a murine leukemia virus (MLV ...
-
bioRxiv - Cell Biology 2022Quote: ... coli DH5⊡ competent cells and isolated using a midi-prep kit (Takara Bio, 740420.10), and validated by gel electrophoresis.
-
bioRxiv - Plant Biology 2021Quote: ... and cells with both constructs were selected on SD/-Leu/-Trp medium (Clontech, 630417). Ten colonies picked up with a tooth pick were diluted in 1ml 0.9%NaCl and spotted onto SD/-Ade/-His/-Leu/-Trp medium (Clontech ...
-
bioRxiv - Neuroscience 2021Quote: ... cells were spinoculated with viral supernatant at 2000xg for 1 hour on retronectin (Takara) coated plates ...
-
bioRxiv - Neuroscience 2020Quote: iPSCs cell culture was performed using the Cellartis DEF-CS 500 culture system (Takara) according to the manufacturers’ instructions ...
-
bioRxiv - Microbiology 2020Quote: ... the cell supernatant was collected and lysed in lysis buffer (Takara, Cat no. 9766) for further quantification analysis ...
-
bioRxiv - Cancer Biology 2020Quote: ... 293T transfected cells conditioned media were condensed with the Lenti-X Concentrator (Clontech: 631231) at 4°C for 24 hours.
-
bioRxiv - Immunology 2022Quote: ... GP2-293 retroviral packaging cells and the p10A1 envelope vector were purchased from Clontech. Lipofectamine 3000 was purchased from ThermoFisher and Polybrene was purchased from Sigma ...
-
bioRxiv - Cancer Biology 2022Quote: ... cells were harvested and AAV2 was isolated using AAVpro Extraction Solution (Takara, Catalog #6235). Two million NC797 cells were infected with 30ul of each adeno-associated virus (AAV2) ...
-
bioRxiv - Pathology 2020Quote: Lentiviruses were packaged in Lenti-X 293T cells (cat#632180, Clontech, Mountain View, CA) using a three-plasmid expression system (pLKO.1-shRNAs-puro ...
-
bioRxiv - Cell Biology 2020Quote: ... target proteins were purified from cell lysate by Ni-iminodiacetic acid affinity chromatography (Clontech). However ...
-
bioRxiv - Microbiology 2020Quote: HeLa cells at 40% confluence on coverslips were transfected with pEGFP-N2 (Clontech, USA) constructs encoding variants of BteA-GFP fusion protein (S3 Table ...
-
bioRxiv - Molecular Biology 2020Quote: ... These cell lines were tested negative for mycoplasma (TaKaRa PCR Mycoplasma Detection Set; 6601), maintained in RPMI1640 (Nacalai Tesque ...
-
bioRxiv - Immunology 2020Quote: ... 106 cells per well were transferred into RetroNectin-coated 24well plates (Takara Bio, USA). Virus supernatant was centrifuged (300xg ...
-
bioRxiv - Genetics 2021Quote: ... HT-29 cells were transduced with the pLVX-Tet3G regulator vector (Takara Bio USA) and a stable Tet-On 3G transactivator-expressing clonal HT-29 cell line (HT-29-pLVX-Tet3G ...
-
bioRxiv - Cancer Biology 2020Quote: Total RNA was isolated from K562 and GM12878 cells using RNAiso Plus (Takara Bio) as per the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2020Quote: ... 10 μl premix WST-1 cell proliferation reagent (Takara Bio Inc, Clontech Laboratories, Inc.) was added to each well ...
-
bioRxiv - Cell Biology 2021Quote: The single-cell sequencing library was generated using the ICELL8 cx platform (Takara Bio) and sequencing was performed using the HiSeq 3000 sequencer (Illumina ...