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Citations for Takara Bio :
1151 - 1200 of 5592 citations for Mouse Antigen peptide transporter 2 TAP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... to OD600 0.2–0.3 with 2–3 mL fresh AYE +Fe +Cys containing 40 ng/mL anhydrous tetracycline (aTC, Clontech 631310). On the third day ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA encoding FLAG-Rhino was cloned into pPB-2× Ty1-Tjen-EGFP-P2A-BlastR51 using In-Fusion cloning (TAKARA), bearing pPB-FLAG-Rhino-Tjen-EGFP-P2A-BlastR ...
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bioRxiv - Neuroscience 2024Quote: ... After the addition of 5 µl lysis buffer (0.2% Triton X-100, with 2 U/μl recombinant RNase inhibitor, Clontech) to the cap ...
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bioRxiv - Evolutionary Biology 2024Quote: ... All yeast 2-hybrid screens were done using the Matchmaker® Gold Yeast Two-Hybrid System (Takara Bio USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... Supernatant containing viral particles was harvested after 2 and 3 days and concentrated 100x using Lenti-X concentrator (Takara) following manufacturers instructions.
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bioRxiv - Cancer Biology 2023Quote: ... His-TEV tagged protein was captured with Co-TALON resin (Clonetech, Takara Bio USA, 2 mL slurry/liter culture) at 4 ºC for 1 h with constant end-to-end mixing ...
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bioRxiv - Plant Biology 2023Quote: ... Yeast transformation was performed as described in the Yeastmaker Yeast Transformation System 2 User Manual (Clontech, TaKaRa Bio, USA). Serial dilution of transformed colonies were dropped on –Trp/-His synthetic dropout selection medium ...
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bioRxiv - Plant Biology 2023Quote: ... Yeast transformation was performed as described in the Yeastmaker Yeast Transformation System 2 User Manual (Clontech, TaKaRa Bio, USA). Serial dilution of transformed colonies were dropped on –Trp/-His synthetic dropout selection medium ...
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bioRxiv - Pathology 2023Quote: ... The bacteria were then collected by centrifugation at 4,000 rpm for 5 min and 2 ml xTractor Buffer (Clontech, TaKaRa Biomedical Technology [Beijing] Co. ...
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bioRxiv - Pathology 2023Quote: ... The bacteria were then collected by centrifugation at 4,000 rpm for 5 min and 2 ml xTractor Buffer (Clontech, TaKaRa Biomedical Technology [Beijing] Co. ...
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bioRxiv - Microbiology 2023Quote: ... the nine pmW118 plasmid vectors were subjected to amplification of the cDNA fragments (F1-F9-10) of SARS-CoV-2 XBB.1 and XBB.1.5 by PrimeSTAR GXL DNA polymerase (Takara) with the primer sets24 ...
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bioRxiv - Genetics 2023Quote: ... The transformation process followed the protocol described in the Yeastmaker™ Yeast Transformation System 2 User Manual (Takara Bio), yielding approximately 2 × 106 and 7 × 106 transformants for LE9 and BLX strains ...
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bioRxiv - Developmental Biology 2022Quote: ... followed by the addition of 2.8 μL quenching buffer (0.7 μL of Triton X-100 [Nacalai Tesque], 0.7 μL of 2% bovine serum albumin [BSA] [Takara Bio]) and incubation at 70°C for 90 sec to quench the denaturing effect of SDc ...
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bioRxiv - Neuroscience 2024Quote: ... in BrainPhys neuronal media (composed according to Bardy et al. 2015)74 and 2 µg/ml doxycycline (Takara 631311). Three days after plating and ...
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bioRxiv - Biochemistry 2023Quote: ... The membrane was incubated for 1 h at room temperature with Odyssey blocking buffer and 0.2 % PBS-T (PBS containing 0.2 % (v/v) Tween-20) with rat anti-HA clone 3F primary antibody (Clontech) at 1:1,000 dilution ...
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bioRxiv - Plant Biology 2023Quote: ... Approximately 2.6 x 106 yeast transformants were screened on 2% SD/Gal/Raf/X-P-gal (-Ura/-His/-Trp/-Leu) following the manufacturer’s instructions (Clontech). Direct protein-protein interaction was confirmed by co-transformation of the respective plasmids into the yeast strain AH109 using the Matchmaker GAL4 Two-hybrid System (Clontech) ...
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bioRxiv - Microbiology 2024Quote: ... The protein-bead mixtures were then loaded onto a gravity column (TALON 2 mL Disposable Gravity Column, Takara #635606). Lysate was loaded into the column 5 times with gravity flow and then washed in a 1M NaCl buffer 3 times ...
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bioRxiv - Microbiology 2020Quote: ... and reverse transcribed using the PrimeScript RT reagent kit (TaKaRa). The GPC segment was amplified and sequenced as previously described (9) ...
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bioRxiv - Cancer Biology 2021Quote: ... with SYBR Premix EX Taq kit (Takara Biotechnology Co., Ltd.). Primer sequences for RRM2 ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was synthesized using the PrimeScript RT Reagent Kit (Takara) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was generated by PrimeScript RT Reagent Kit (Takara RR037A). Real-time PCR was performed on 15 ng of cDNA using SYBR Premix Ex Taq (Tli RNaseH Plus ...
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bioRxiv - Cell Biology 2020Quote: ... with SYBR Premix Ex Taq II Kit (Clontech Laboratories, USA) and primers presented in Supplemental Table 2 ...
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bioRxiv - Genetics 2021Quote: ... Illumina small RNA-Seq kits (Clontech, Mountain View, CA, USA) were then used to generate cDNA libraries ...
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bioRxiv - Developmental Biology 2021Quote: ... SMART-Seq v4 Ultra-Low Input RNA kits (TaKaRa Bio) were used to generated polyA selected libraries from total RNA ...
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bioRxiv - Neuroscience 2021Quote: ... using CalPhos™ Mammalian Transfection Kit (Takara Bio or Promega) on DIV7 ...
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bioRxiv - Developmental Biology 2020Quote: ... and young adult) using the PrimeScript RT Reagent Kit (Takara). RT-qPCR was performed in a StepOnePlus(tm ...
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bioRxiv - Neuroscience 2021Quote: ... The SMART-Seq v4 Ultra Low Input RNA Kit (Clontech) was used for library preparation ...
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bioRxiv - Genomics 2020Quote: ... and the Cellartis® DE Differentiation Kit (Takara Bio Inc.), respectively ...
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bioRxiv - Developmental Biology 2019Quote: ... cDNA was synthesized using the PrimeScript RT Reagent Kit (Takara). qPCR was conducted using SYBR Premix Ex Taq (Takara) ...
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bioRxiv - Genomics 2019Quote: ... and the recently released RiboGone Mammalian kit (Takara Bio/Clontech). The latter combines hybridization of rRNA and mtRNA baits with RNAse H digestion ...
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bioRxiv - Genomics 2019Quote: ... and the recently released RiboGone Mammalian kit (Takara Bio/Clontech). The latter combines hybridization of rRNA and mtRNA baits with RNAse H digestion ...
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bioRxiv - Cell Biology 2019Quote: ... digested by BamHI using In-fusion HD cloning kit (Clontech Laboratories ...
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bioRxiv - Genomics 2019Quote: ... enhancer assay vector using In-Fusion HD cloning kit (Takara) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ... In-Fusion cloning (In-Fusion HD Cloning Plus Kit, Clontech) was used to fuse the fragments with the linearized backbone ...
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bioRxiv - Genomics 2021Quote: ... PrimeScript High Fidelity RT-PCR Kit (Takara Bio, Japan, R022A), In-Fusion Snap Assembly Master Mix (Takara Bio ...
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bioRxiv - Developmental Biology 2021Quote: ... and converted to cDNA by PrimeScript Reverse Transcriptase Kit (Takara). The resulting cDNA was subjected to PCR or qPCR analysis using Phire Green Hot Start II PCR Master Mix (Promega ...
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bioRxiv - Genomics 2021Quote: ... and reverse transcribed using the PrimeScript RT Reagent Kit (Takara). qPCR was performed using primers listed in Table 1.
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bioRxiv - Synthetic Biology 2021Quote: ... BAC DNA was isolated using Nucleobond XtraBAC kit (Takara 740436.25) according to manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... cDNA libraries were obtained using SMARTseq v4 kit (Clontech, # 634888) and sequenced using HiSeq 2500 sequencer ...
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bioRxiv - Developmental Biology 2021Quote: ... linearised with NheI using the InFusion kit (638909, Takara Bio). Fertilised single-cell embryos were injected with 30 pg of plasmid DNA and 25 pg of in vitro transcribed Ac mRNA (using mMESSAGE mMACHINE SP6 Transcription Kit ...
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bioRxiv - Microbiology 2021Quote: The Clontech SMARTSeq v4 Ultra-Low Input RNA kit (Takara) was used with 1.7 ng of input RNA from each sample ...
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bioRxiv - Cell Biology 2021Quote: ... The cDNA was synthesized using Primescript RT reagent kit (TakaRa), and qPCR analysis was carried out (14 ...
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bioRxiv - Cell Biology 2021Quote: ... Truncations were made using the InFusion HD Cloning Kit (Takara). The same construct was cloned into a modified pET-Duet-1 vector that contained an HRV protease cleavable N terminal 6x His-tag ...
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bioRxiv - Cancer Biology 2020Quote: ... using the SMARTer™ ICELL8 3’ DE Kit (Takara Bio). Processing 8 samples at a time ...
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bioRxiv - Neuroscience 2020Quote: ... or with EGFP (Control) using a CalPhos Transfection Kit (Takara) at DIV10 and immunostained at DIV14 ...
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bioRxiv - Physiology 2021Quote: ... and gel extracted (Clontech Nucleospin Extract II Kit Cat# 636972). The linearized and purified pMLM313 fragment was used as template for in vitro transcription of Cas9 mRNA with mMESSAGE mMACHINE T7 ULTRA kit (Life Technologies)16 ...
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bioRxiv - Plant Biology 2021Quote: ... We used the SMARTerTM RACE cDNA Amplification Kit (634923, Clontech) to determine the 5’- and 3’–ends using WTK4-specific primers (Supplementary Table 12 ...
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bioRxiv - Cell Biology 2021Quote: ... for doxycycline-inducible expression using InFusion HD cloning kit (TaKaRa). Additional GGGS linker was introduced between the Cry2olig and caspase/kinase domains or Cry2olig and mCherry to reduce potential sterical interference between domains ...
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bioRxiv - Microbiology 2021Quote: ... using the In-Fusion HD cloning kit (Clontech, Kusatsu, Japan) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... in Lysis buffer from NucleoSpin RNA Plus Kit (Clontech, USA). RNA from the lung homogenate or macrophages was isolated using NucleoSpin RNA Plus Kit (Clontech ...