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Citations for Takara Bio :
1101 - 1150 of 1431 citations for 1 Methoxy 4 1 methylbutyl benzene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... or the expression vector LentiCRISPRv2 (coding for guideRNA) in a 3:1:3 ratio for 8 hours using calcium phosphate transfection (CalPhos Mammalian Transfection Kit, Takara Bio Europe ...
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bioRxiv - Cell Biology 2022Quote: ... The yeast strain AH109 was transformed with the constructs in destination vectors using lithium acetate transformation (Yeast Protocols Handbook PT3024-1, Clontech). The transformants were grown on synthetic defined medium (0.67% yeast nitrogen base ...
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bioRxiv - Molecular Biology 2022Quote: ... Lysate was cleared by centrifugation at 43 550×g and afterwards bound to 1 mL 50% slurry of Talon Metal Affinity Resin (TaKaRa) equilibrated in lysis buffer without PMSF ...
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bioRxiv - Cancer Biology 2022Quote: Telomerase-mediated extension and subsequent amplification of TRAP products were conducted in 25-µL reactions containing 1 µL of cell lysate and 2 U of Titanium Taq DNA polymerase (Takara). The other kit components—TRAP reaction buffer ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA was amplified by PCR by adding 1 µl of the Terra PCR Direct Polymerase (1.25 U/µl, Takara 639270), 25 µl of the Terra PCR Direct buffer and 1 µl of the ISPCR primer (10 µM stock concentration ...
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bioRxiv - Immunology 2022Quote: ... following manufacturer’s instructions with a 1.5:1.5:1 μg ratio of the SFG:Peq-Pam:RD114 DNA plasmids and concentrated using Retro-X™ Concentrator (Takara, Kusatsu, Japan) following manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: ... with 1 μg of total RNA as input for the SMARTer Apollo NGS library prep system (Takara, Mountain View, CA). The library for RNA-seq was prepared by using NEBNext Ultra II Directional RNA Library Prep kit (New England BioLabs ...
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bioRxiv - Systems Biology 2022Quote: ... cDNA was synthesized from 1 μg of total RNA using the PrimeScript RT reagent kit (Perfect Real Time) (Cat No RR037A, TaKaRa) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... First-strand cDNA was synthesized from 1 μg of total RNA using the PrimeScript™ RT Master Mix Kit (Takara). SYBR Premix Ex Taq™ kit (Takara ...
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bioRxiv - Plant Biology 2023Quote: ... cDNAs were prepared from 1 ng of total RNA using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (TaKaRa) according to the manufacturer guidelines for cDNA synthesis and amplification ...
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bioRxiv - Microbiology 2022Quote: ... HeLa wild-type or HeLa-HSF1i cell monolayers were mock-transfected or co-transfected with HCoV genomic RNA (1 μg/ml) and pCMV-GFP vector (Clontech) using TransIT-mRNA Transfection Kit (Mirus Bio ...
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bioRxiv - Molecular Biology 2022Quote: ... The cDNAs (1-5 ng RNA equivalents) were used for real-time PCR amplification using SYBR Premix Ex Taq II (Takara/Clontech) on a 7500 Fast Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Plant Biology 2022Quote: ... 0.1 g of leaves or fruits was mixed with 1 mL RNAiso plus according to manufacturer’s instruction (Takara, Kusatsu, Japan), then RNA was reverse-transcribed into cDNA using PrimeScript RT reagent with gDNA Eraser (Takara ...
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bioRxiv - Immunology 2022Quote: ... T cells were infected with either replication-competent HIV-1 (pAD8gNef [80] or pNLgNef [79] or single-round HIV-1 (pHR-CD8a-d2eGFP-IRES-Nef) using Lenti-X Accelerator (TaKaRa) following the manufacturer’s protocol.
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bioRxiv - Immunology 2022Quote: ... HEK 293T cells were seeded in a 6-well plate at a density of 6 × 105 cells/well and co-transfected with pSIN-siU6 – shSLFN11/ shSLFN12/ shSc (2 μg) along with plasmids pGP (1 μg, Takara) and pPE ampho (1 μg ...
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bioRxiv - Microbiology 2022Quote: ... 1 ng of total RNA was pre-amplified with the SMARTer Ultra Low Input kit v4 (Clontech, Mountain View, CA) per manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 μg of total RNA was reverse transcribed into complementary DNA (cDNA) using PrimeScript™ RT Reagent Kit (Takara, RR047B). Amplification of cDNA product was performed using specific primers with the TB Green® Premix Ex Taq™ II (Takara ...
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bioRxiv - Microbiology 2024Quote: ... The PCR product was run on a 1% agarose gel at 85 V for 35 min and gel extracted using the Nucleospin Gel Extraction Kit (Takara). We then used PCR with KOD Polymerase 2x Mastermix (Millipore Sigma ...
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bioRxiv - Microbiology 2024Quote: ... the first strand primer was annealed to 1 µl of sample RNA before being extended with SMARTScribe reverse transcriptase (Clontech). After the addition of the second strand primer ...
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bioRxiv - Immunology 2024Quote: ... we ran our linearized plasmid backbone and 83 bp PCR products on a 1% agarose gel and recovered each using the Gel and PCR Clean-up Kit (Takara) per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Eukaryotic expression vectors encoding green fluorescent protein (GFP)-tagged proteins were generated by inserting PCR-amplified fragments into pd1-EGFP-N1 vector (6073-1, Clontech). Prokaryotic plasmids encoding GST-fusion proteins were constructed using pGEX-4T-1 bacterial expression vector (27-4580-01 ...
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bioRxiv - Biochemistry 2023Quote: ... The PCR product was inserted into a BamHI and XhoI double-digested pGEX-6P-1 vector using InFusion Snap Assembly (Takara), resulting in the expression construct for GST-3C-GCP21-110 (referred to as “GST-GCP2-NHD”) ...
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bioRxiv - Cell Biology 2024Quote: A mix of 4 μg of pcDNA3.1 plasmid containing the mouse Wnt10b cDNA (provided by Dr Bernardi, INRA, Montpellier) and 1 μg of pAcGFP-Nuc that encodes a nuclear GFP (Clontech) in 0.9% NaCl was electrotransfected directly into TA muscles of anesthetized ob/+ and ob/ob mice according to the protocol described by Grifone et al.[29] ...
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bioRxiv - Immunology 2022Quote: ... Sequences encoding DCFHP (residues 1-1146 of HexaPro)2 and SΔC-Fer (residues 1-1143 as previously described)3 were cloned into the pADD2 vector backbone using HiFi PCR (Takara) followed by In-Fusion (Takara ...
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bioRxiv - Neuroscience 2023Quote: ... GFP-T42EM was created by cloning T42EM (Ding et al., 2006) into the HindIII and BamHI sites of the pEGFP-C1 plasmid (6084-1, Clontech). To verify tau protein expression ...
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bioRxiv - Plant Biology 2023Quote: ... the 1707 bp upstream sequence from the +1 site of the OsEG45 gene was cloned into the KpnI and SacI RE sites of the pAbAi vector (Takara). The OsGF14e gene was cloned into the pGADT7-Rec vector (Takara) ...
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bioRxiv - Cell Biology 2022Quote: ... Wild-type iRhom2 and iRhom2-1-374 were also cloned by PCR into pM6P.Blasticidin (for retroviral infection) and into pLVX-TetOne-Puro vector (Clontech, #631849) (for lentiviral infection) ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 × 106 HEK293T cells were transfected with 7 μg of Env expressor and 1 μg of a green fluorescent protein (GFP) expressor (pIRES2-EGFP; Clontech) with the calcium phosphate method ...
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bioRxiv - Biochemistry 2023Quote: ... The constructs CBSΔ516–525 and CBSCD (residues 1-413) along with single point mutations of CBS were introduced using In-Fusion (Takara) or QuikChange (Agilent ...
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bioRxiv - Cell Biology 2022Quote: ... The viral supernatant was passed through a 0.45 um filter and concentrated to 1:66 with Lenti-X concentrator (631231, Takara Bio) in house or to 1:255 by ultracentrifugation in the core.
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bioRxiv - Immunology 2023Quote: The medium from transfected HEK293T cells was replaced with Opti-MEM containing 1 μM of B/B Homodimeriser (AP20187; Clontech) and incubated for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... Reverse transcription (RT) reactions were performed with 1 μg total RNA using the PrimeScript RT kit with gDNA Eraser (Takara). The resulting cDNA was amplified in triplicate on the 480 Real-Time PCR system (Roche ...
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bioRxiv - Systems Biology 2023Quote: ... were delivered by spinfection into 100k cells using 1 mL of lentivirus that was concentrated 5x with LentiX Concentrator (Takara). 4 days later ...
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bioRxiv - Systems Biology 2023Quote: ... were delivered by spinfection into 100k cells using 1 mL of unconcentrated lentivirus or virus that was concentrated 5x with LentiX Concentrator (Takara). Blasticidin was added at day 3 or 5 to select for dCas9 delivery ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Reverse transcription (RT) reactions were performed with 1 μg total RNA using the PrimeScript RT kit with gDNA Eraser (Takara). The resulting cDNA was amplified in triplicate on the 480 Real-Time PCR system (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... Two-color FISH was conducted as previously described with minor adaptations.20,21 Undifferentiated ChiPSC22 were seeded on DEF-CS COAT-1-coated (Cellartis, Takara BioSciences) coverslips and differentiated ChiPSC22 on poly-L-lysine-coated coverslips ...
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bioRxiv - Biochemistry 2023Quote: The gene encoding XccOpgD (GenBank: AAM43366.1) was amplified by PCR with the primer pair shown in Supplementary Table 1 using PrimeSTAR Max (Takara Bio) and a genomic DNA of X ...
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bioRxiv - Plant Biology 2023Quote: ... and the input and pull-down prey proteins were detected by immunoblot using anti-His (M201, Takara, 1:3000 dilution) and anti-GST (G018 ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µl of unpurified RT product was amplified in 12.5 µl using the Advantage HF 2 PCR Kit (Takara Bio) with 1X Advantage 2 PCR Buffer ...
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CTCF regulates global chromatin accessibility and transcription during rod photoreceptor developmentbioRxiv - Genomics 2024Quote: ... and 1 ng total RNA was used to generate RNA-seq libraries with SMARTer Stranded Total RNA-Seq Kit v3 (Takara). All samples were sequenced with NovaSeq 6000 (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... Both flanking arms and the GFP coding sequence were amplified from viral DNA extracted from LSDV infected MDBK cells and pEGFPN-1 (Clontech) plasmid ...
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bioRxiv - Microbiology 2024Quote: ... the PrimeScript 1st strand cDNA Synthesis Kit with gDNA Eraser was used to reverse transcribe 1 μg total RNA onto the first strand of cDNA in each pool for qPCR (TaKaRa). qRT-PCR was carried out using a 10 μL reaction volume consisting of 5 μL qPCR Mix (Low ROX ...
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bioRxiv - Microbiology 2024Quote: ... HEK293T cells were co-transfected with the resulting vector pLVX-Puro-GFP-1-10 and the packaging and envelope plasmids using Lenti-X Packaging Single Shots (Takara). Supernatants containing lentiviral particles were collected at 48 and 72 hours post-transfection ...
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bioRxiv - Developmental Biology 2024Quote: ... 50 ng-1 ug of mRNA was reverse transcribed to cDNA using the PrimeScript RT Master Mix kit (Takara, RR036A). Real-time PCR was performed using the TB Green Premix Ex Taq kit (Takara ...
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bioRxiv - Molecular Biology 2024Quote: ... Expression of tagged proteins was induced overnight by the addition of doxycycline (Clontech, 8634-1, 10 ng/mL final concentration) and all clones were verified by western blotting using antibodies against flag.
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bioRxiv - Pathology 2024Quote: ... ENTV-1 and ENTV-2 were amplified by PCR (Table 2) using the high-fidelity DNA polymerase “PrimeStar GXL” (Takara), with primers targeting exogenous β-retroviruses ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... using gene-specific oligonucleotide primers encompassing AsiSI and MluI restriction sites (Table 1) and Advantage Polymerase mix (Takara Bio, USA). The AsiSI-MluI-digested rTRPV1 DNA fragment was ligated into the pcDNA™5/FRT/TO plasmid (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... Complementary oligonucleotides (0.1 nmole each) corresponding to the sgRNA sequences (LpBBS1gRNA3’UTR48F and LpBBS1gRNA3’UTR48R) were phosphorylated by T4 polynucleotide kinase (TAKARA), annealed ...
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bioRxiv - Genomics 2024Quote: ... 1X Terra PCR Direct Buffer and 1 uL of 1.25 U/uL Terra PCR Direct Polymerase Mix (639270, Takara Bio). To clean up the reaction ...
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bioRxiv - Cell Biology 2024Quote: ... pLVX- Neo:Eto.1 was a gift from the Fred Gage lab (Salk Institute) and is pLVX-Tet-On Advanced (Clontech/Takara) with the CMV promoter replaced with the EF1a promoter ...