Labshake search
Citations for Takara Bio :
1001 - 1050 of 6065 citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... facilitated by the PrimeScript RT regent Kit (TaKaRa, Japan). Purification of the synthesized second-stranded DNAs was accomplished using AMPureXP beads ...
-
bioRxiv - Molecular Biology 2024Quote: ... cDNA was generated using PrimeScript RT kit (Takara RR037A) with 100-500 ng RNA as input ...
-
Pyruvate kinase M2 regulates Japanese encephalitis virus replication by interacting with NS1 proteinbioRxiv - Microbiology 2024Quote: Capturem Co-IP kit was procured from TaKaRa (Japan). The Neuro-2a cells were transfected with pEGFP-PKM2 and cells were infected with JEV 24 hours post-transfection ...
-
bioRxiv - Cell Biology 2024Quote: ... packaging plasmid using the CalPhos Mammalian Transfection Kit (Takara). The next day ...
-
bioRxiv - Genomics 2024Quote: ... using the In-Fusion HD Cloning Kit (Takara Bio) and following the manufacturer’s instructions ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... using the Advantage® 2 PCR Kit (Takara Bio) in a touchdown cycling program as follows ...
-
bioRxiv - Plant Biology 2019Quote: 6His-GST-CRK2cyto and 6His-MBP-RBOHD/C constructs for recombinant proteins were generated by using In-Fusion technology (Clontech). The coding regions of CRK2cyto (WT ...
-
bioRxiv - Cell Biology 2021Quote: ... Then the homology arms and fluorescent protein sequences were assembled and cloned into the BamHI site of a pUC19 vector using Gibson assembly (Clontech). The primer sequences for the mGFP-EB1 ...
-
bioRxiv - Developmental Biology 2020Quote: Doxycycline-inducible overexpression of HA-tagged proteins was achieved using the Lenti-X Tet-On 3G Inducible Expression System (Clontech) following the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2021Quote: ... as described previously (Keith et al., 2012, Freund et al., 2016) with plasmids encoding green fluorescent protein (GFP) (pEGFPN1; Clontech), mouse Kif11 (Myers and Baas ...
-
bioRxiv - Molecular Biology 2021Quote: Stable 3T3 cells (ATCC) overexpressing zDHHC3 or green fluorescent protein (GFP) as a control were generated using the pLVX lentiviral system (Clontech). Cells were labeled by stable isotope labeling with amino acids in cell culture (SILAC ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Genes of target prokaryotic proteins were amplified by PCR from corresponding genomic DNA and inserted into the pEGFP-C1 vector (Clontech). Mutated genes of prokaryotic proteins were obtained by PCR site-specific mutagenesis ...
-
bioRxiv - Microbiology 2021Quote: 293T were transfected with full length SARS-CoV-2 Spikes and a green fluorescent protein (GFP) expressor (pIRES2-eGFP; Clontech) using the calcium-phosphate method ...
-
bioRxiv - Biochemistry 2022Quote: ... The induction of His-and GST-fusion proteins and their subsequent purification using TALON® Metal Affinity Resin (Clontech Laboratories) and Glutathione SepharoseTM 4 Fast Flow beads (GE Healthcare) ...
-
bioRxiv - Biochemistry 2022Quote: ... Recombinant His-tagged protein was purified from cell-free extracts using immobilised metal affinity chromatography (IMAC using Talon resin; Clontech) as described previously (34) ...
-
bioRxiv - Microbiology 2022Quote: ... PreS2-LHBS sequence (deletion nucleotides 2-55) was cloned into the protein stability construct after the c-terminal of EGFP by In-Fusion cloning (Takara). The protein stability construct was a kind gift from Dr ...
-
bioRxiv - Plant Biology 2022Quote: ... The immunoprecipitated (RGA-GFP and RGAm2-GFP) and co-immunoprecipitated (IDD2-RFP) proteins were detected by western-blot with anti-GFP (JL8; Clontech) and anti- RFP (6G6 ...
-
bioRxiv - Cancer Biology 2020Quote: ... the TET protein expression in each clone was checked by immunoblotting using TetR monoclonal antibody (Clone 9G9) (Clontech, cat#631131). In addition ...
-
bioRxiv - Physiology 2022Quote: ... the beads were well-washed by column binding buffer 5 times and then incubated in 2× Protein SDS PAGE Loading (Takara) 100°C for 5 min to completely elute the proteins ...
-
bioRxiv - Microbiology 2022Quote: ... Plasmid pTAP-AFAP was constructed by cloning the gene fragment encoding AFAP-streptavidin-binding peptide-3xFLAG tag fusion protein (AFAP-SBP-3xFLAG, shorted as AFAP-SF) into mammalian expression vector pIRESpuro3 (Takara). SBP is a peptide with the length of 38 amino acids (Wilson et al. ...
-
bioRxiv - Molecular Biology 2019Quote: ... The cell debris was removed upon centrifugation and the proteins were purified from the supernatant by His-tag affinity chromatography using Ni-NTA agarose beads (Clontech). The bound proteins were washed with lysis buffer containing 10 mM imidazole and then eluted with lysis buffer containing 250 mM imidazole ...
-
bioRxiv - Biochemistry 2019Quote: ... The cell debris was removed upon centrifugation in a SS34 rotor at 4 °C (27,000 × g for 15 min) and the proteins were purified from the supernatant by His-tag affinity chromatography using Ni-NTA agarose beads (Clontech). The bound proteins were washed with lysis buffer containing 25 mM imidazole and then eluted with lysis buffer containing 500 mM imidazole ...
-
bioRxiv - Biophysics 2020Quote: ... 6xHis-tagged protein fraction was incubated for thirty minutes at 4°C with Talon resin (Clontech Laboratories/Takara Bio USA) that had been previously equilibrated in lysis/extraction buffer ...
-
bioRxiv - Biophysics 2020Quote: ... 6xHis-tagged protein fraction was incubated for thirty minutes at 4°C with Talon resin (Clontech Laboratories/Takara Bio USA) that had been previously equilibrated in lysis/extraction buffer ...
-
bioRxiv - Microbiology 2020Quote: ... Recombinant protein were purified to >90% electrophoretic purity by immobilized metal ion affinity chromatography using a cobalt-based matrix (Talon, Clontech) and eluted with imidazole as described previously24 ...
-
bioRxiv - Molecular Biology 2020Quote: PJ69-4a strain of Saccharomyces cerevisiae was used for the expression of proteins of interest using the Matchmaker Gal4-based yeast two hybrid system (Clontech). Tested bait/prey combinations were co-transformed into PJ69-4a and cultivated at 28°C ...
-
bioRxiv - Microbiology 2021Quote: ... The pcDNA3.4 expression vector containing the sequence that encodes the His-tagged extracellular domain of the spike protein was transfected into Expi293 cells and the His-tagged spike protein produced in the culture supernatants was then purified with a Talon resin (Clontech).
-
bioRxiv - Cell Biology 2020Quote: ... generates a protein that undergoes spontaneous aggregation that reverses upon the addition of D/D solubilizer (catalogue# 635054; Takara Bio), a cell-permeant rapamycin analog that binds to the FM4 domains (see Fig 3e).
-
bioRxiv - Immunology 2020Quote: ... For binding studies the 6xHis tag were removed from some Fabs by treatment with PreScission protease (MolBioTech; ThermoScientific) and the protein repurified on cobalt-nitrilotriacetic acid (Co-NTA) agarose (Clontech) followed by gel filtration chromatography on Superdex 200 (GE Healthcare ...
-
bioRxiv - Molecular Biology 2019Quote: A DNA fragment containing CDS of each Hero protein and C-terminal FLAG and His tags was inserted into pCold I (Takara) by NEBuilder HiFi DNA Assembly Master Mix (NEB) ...
-
bioRxiv - Cell Biology 2022Quote: ... Plasmids used for expression of fluorescent proteins in hTert-RPE1 cells are derivatives of the plasmid pEGFP-C1 and pmCherry-C1 (Clontech). Plasmids were analyzed by sequencing ...
-
bioRxiv - Cancer Biology 2022Quote: ... was modified to replace the puromycin-resistance gene with the enhanced green fluorescent protein (EGFP) gene using the In-Fusion Snap Assembly Master Mix (TaKaRa). Forty-eight hours after transfection ...
-
bioRxiv - Microbiology 2022Quote: ... His-tagged AmiC was isolated from the protein sample using a TALON® metal affinity resin (Takara Bio USA, Inc) as previously described (51 ...
-
bioRxiv - Microbiology 2023Quote: A yeast screen for human cellular interacting proteins of pUL136 was performed using the Matchmaker Gold Yeast Two-Hybrid System (Clontech) and the Mate and Plate Universal Human Library (Clontech) ...
-
bioRxiv - Biophysics 2022Quote: ... These plasmids were designed by fusing the coding sequences of the respective proteins C-terminal (intracellularly) via a linker to mEGFP (called D0 / Donor only) or mCherry in the pIRESpuro2 vector (Clontech)32 (for more linker details see Supplementary Table 5) ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... The recombinant proteins were purified using a TALON Metal (Cobalt) Affinity Resin column (Clontech Laboratories, Inc., Palo Alto, CA, USA) and eluted with a linear gradient of imidazole (0–1,000 mM ...
-
bioRxiv - Plant Biology 2024Quote: The HIS3 and ADE2 reporter genes were employed to assess the protein interactions in the yeast strain Y2HGold (Takara Bio). Yeast transformation was carried out using the lithium acetate (LiAc)-mediated method ...
-
bioRxiv - Genomics 2024Quote: ... The cells were infected with prepackaged lentiviral particles (constitutive reporter vector expressing tdTomato fluorescent protein gene driven by EF1a promoter (Takara) at MOI of 20 (Stock ...
-
bioRxiv - Neuroscience 2024Quote: ... HEK293 cells in 24-well plates were transfected with a total of 0.9 μg of DNA vectors carrying genes encoding GluN1 and GluN2 subunits and green fluorescent protein (GFP; pQBI 25 vector; for identification of transfected cells; Takara) in a 1:1:1 ratio using 0.9 μl of PolyMag reagent (OZBiosciences ...
-
bioRxiv - Biochemistry 2024Quote: ... Recombinant His-tagged protein was purified from cell free extracts in a single step using immobilized affinity chromatography (IMAC) with Talon resin (ClonTech) and dialyzed overnight into 20 mM Tris-HCl ...
-
bioRxiv - Biochemistry 2024Quote: ... pLysS or Rosetta pLysS cells and overexpressed proteins were purified using immobilized metal-ion affinity chromatography (IMAC) with TALON resin (Clontech) (Abad et al ...
-
bioRxiv - Microbiology 2019Quote: ... 1 × GC buffer II and 1 U of LA Taq DNA polymerase (TaKaRa). The PCR cycles consisted of 95 °C for 5 min ...
-
bioRxiv - Biochemistry 2022Quote: ... TEX12 (1-123) and SIX6OS1 (1-587) were cloned into pGADT7 vectors (Clontech). The Matchmaker™ Gold system (Clontech ...
-
bioRxiv - Molecular Biology 2020Quote: ... The plug was equilibrated twice in 1 ml of 1× M buffer (TaKaRa) by rotating the tube for 30 min at room temperature ...
-
bioRxiv - Neuroscience 2020Quote: ... then in 1:25,000 or 1:100,000 rabbit anti DSRed (Takara Bio USA) in 10% NHS-TPBS ...
-
bioRxiv - Microbiology 2022Quote: ... and 50 ng mL−1 or 25 ng mL−1 anhydrotetracycline (aTc, Clontech) and Aeromonas sp ...
-
bioRxiv - Molecular Biology 2024Quote: ... The plug was equilibrated twice in 1 mL of 1× M buffer (TaKaRa) by rotating the tube for 30 min at room temperature ...
-
bioRxiv - Physiology 2024Quote: ... (Cx43: 1:3000 custom-produced rabbit polyclonal; eGFP: 1:1000 mouse monoclonal (Clontech Laboratories Inc ...
-
bioRxiv - Molecular Biology 2024Quote: ... The plug was equilibrated twice in 1 mL of 1× M buffer (TaKaRa) by rotating the tube for 30 min at room temperature ...
-
bioRxiv - Molecular Biology 2022Quote: cDNA was prepared from EV RNA using the Single Cell RNA kit SMART-Seq_V4 Ultra Low Input RNA Kit (634890, Takara, Mountain View, CA, USA) according to manufacturer instructions using 1ng input ...