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Citations for Takara Bio :
951 - 1000 of 5191 citations for Total Carbohydrate Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... Liquid β-galactosidase assays were performed as described in the Yeast Protocols Handbook (Clontech). A Y2HGold yeast strain containing a bait vector was mated with the Y187 strain containing the pGADT7 vector and selected on SD/-Trp/-Leu media ...
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bioRxiv - Microbiology 2022Quote: ... The RT-qPCR assay was performed using the TB Green Fast qPCR Mix (Takara). The ribosomal protein L21 gene (RPL21 ...
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bioRxiv - Plant Biology 2022Quote: Yeast one-hybrid assays were performed using the Matchmaker Yeast One-Hybrid System (Clontech). The DNA fragment 1,000 bp upstream of the start codon ATG of DDRM2 was cloned into pHis2.1 vector and SOG1 was cloned into the pGADT7 vector ...
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bioRxiv - Microbiology 2023Quote: RNA obtained from the RIP assay sample was purified using NucleoSpin RNA Plus (TaKaRa), followed by library construction and RNA-seq analysis ...
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bioRxiv - Plant Biology 2023Quote: Y2H assays were performed with the MatchMaker GAL4 Two-Hybrid System 3 (Takara Bio). Saccharomyces cerevisiae strain AH109 was co-transformed with different pairs of pGADT7 and pGBKT7 harboring IREH1 or B1-Rafs ...
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bioRxiv - Plant Biology 2023Quote: ... the Y1H assays were performed following the manufacturer’s instruction (Clontech Laboratories, Inc., Kusatsu, Japan). The promoter sequence with and without mutation in ZDRE motif were artificially synthesized and cloned into the Kpn I-linearized pAbAi vector (Clontech Laboratories ...
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bioRxiv - Immunology 2023Quote: ... As a negative control for pull-downs assays the eGFP plasmid (Clontech Laboratories, USA) was transfected.
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bioRxiv - Biochemistry 2024Quote: ... Protein concentration was determined by bicinchoninic acid assay using bovine serum albumin (TaKaRa Bio) as the reference standard ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was synthesized from 500 ng of total RNA using PrimeScript RT Master Mix (Takara). Quantitative real-time PCR (qPCR ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell homogenates were then subjected to total RNA extraction with Nucleospin RNA plus (Takara Bio) according to the manufacturer’s instructions.
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bioRxiv - Pathology 2022Quote: Total RNA was extracted from skin or cell lysates using TRIzol Reagent (RNAiso Plus, TaKaRa). From isolated RNA ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was extracted from imbibed seeds using RNAiso plus reagent (TaKaRa, Shiga, Japan, Cat9109) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Total RNA or purified mRNA was reverse transcribed with PrimeScript™ RT Master Mix (Takara) using Oligo dT primers to obtain complementary DNA ...
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bioRxiv - Cancer Biology 2019Quote: Total RNA was isolated from stably transduced cell lines using RNAiso Plus (TaKaRa, Dalian, China) and reverse-transcribed with a PrimeScript™ cDNA Synthesis Kit (TaKaRa ...
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bioRxiv - Immunology 2019Quote: ... cDNA was prepared from total RNA preps using the RNA to cDNA EcoDry Premix (Clontech). Real-time assays were conducted using TaqMan real-time probes (Applied Biosystems) ...
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bioRxiv - Neuroscience 2021Quote: ... Total RNA (1 µg) was reverse-transcribed with PrimerScript™ RT Master Mix (Takara, #RR047A) after DNase I treatment (Takara ...
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bioRxiv - Genetics 2021Quote: ... The purified RNA (total 30 μg) was reverse transcribed using PrimeScript RT Master Mix (Takara). On-target or off-target sites of genomic DNA or cDNA were amplified with KOD Multi & Epi PCR kits (Toyobo ...
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bioRxiv - Physiology 2021Quote: ... PCR was performed with cDNA derived from 0.5 μg of total RNA with Extaq (TaKaRa). The primer sequences were 5’-CAACGCCCATCGTTTCATGG-3’ and 5’-CAAAAACTTGAAGCCCGCCA-3’ for NtGAox20 and 5’-ATGTGTTCGTTTCAGCCCGA-3’ and 5’-CCGCTGAAAAGTGTGCTTCC-3’ for tobacco arcA as an internal gene control (Ishida et al. ...
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bioRxiv - Cell Biology 2021Quote: The cDNA encoding human BORCS6 was cloned from human lung total RNA (Takara Bio, Japan) and inserted into pCR4-TOPO (Invitrogen ...
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bioRxiv - Pathology 2022Quote: ... 200 ng of total RNA was reverse transcribed using cDNA Synthesis Mix (Takara, Dalian, China). Real-time PCR was carried out in an Applied Biosystems QuantStudio 5 Real-Time PCR System (ABI ...
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bioRxiv - Microbiology 2022Quote: Total RNA from mouse corneas was extracted by the RNAiso plus reagent (TaKaRa, Dalian, China). RNA samples were reverse transcribed using HiScript III RT SuperMix (Vazyme ...
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bioRxiv - Biochemistry 2021Quote: ... total DNA was extracted from muscle tissue using Lysis Buffer for PCR (Takara, Shiga, Japan) and Proteinase K (Takara) ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA was extracted from cells using the TRIzol RNA isolation reagents (Takara bio, Japan). The first strand cDNA was reverse transcribed from the RNA templates using the GoScript Reverse Transcription system kit (Promega ...
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bioRxiv - Molecular Biology 2019Quote: ... One microgram of total RNA was reverse transcribed with Prime ScriptTM RT Master Mix (TaKaRa). Quantitative PCR assays were performed on a qTOWER apparatus (Analytic Jena ...
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bioRxiv - Genomics 2019Quote: ... We mixed 2 µg of total RNA with 1 µl 10 mM dNTPs (Clontech #639125) and 1 µl of 50 µM SMART_dT18VN primer (for a complete list of primer sequences ...
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bioRxiv - Physiology 2019Quote: The total RNA from the various samples were extracted using RNAiso plus (TaKaRa, Dalian, China) and the first-strand cDNA was synthesized using HIScript® Q Select RT SuperMix for qPCR (Vazyme ...
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bioRxiv - Plant Biology 2019Quote: Total RNA was isolated from 200 mg fresh leaf using an RNAiso Plus (Takara, DALIAN), and cDNA was synthesized using the GoScript Reverse Transcription System (Promega) ...
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bioRxiv - Molecular Biology 2021Quote: ... 200-500 ng of total RNAs were reverse transcribed using Primescript RT master mix (Takara). Quantitative PCR was performed using Power SYBR Green PCR Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... Six hundred thirty nanograms of total RNA was annealed to random 9-mer primers (TaKaRa) and reverse-transcribed using ProtoScript II (New England Biolabs) ...
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bioRxiv - Cancer Biology 2020Quote: ... 500ng of total RNA were retrotranscribed using PrimeScript RT Master Mix (Takara Bio, Cat. # RR036A), and subsequent TaqMan assay-based or SYBR Green-based qPCRs were performed using PerfeCTa qPCR ToughMix (QuantaBio ...
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bioRxiv - Neuroscience 2022Quote: ... and either 4 wells of 10 pg of Human Universal Reference Total RNA (Takara 636538) or 2 wells of 10 pg of Human Universal Reference and 2 wells of 10 pg Control RNA provided in the Clontech kit ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA was extracted from cells or frozen tissues using the TRIzol Reagent (Takara, Japan). The complementary DNA (cDNA ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was extracted from three-week-old plants with Trizol reagent (9109, Takara, Japan). The RNA was treated with gDNA Eraser (RR047A ...
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bioRxiv - Cell Biology 2023Quote: ... and SOX4 were amplified from a reverse transcript of Human Fetal Brain Total RNA (Takara) by high-fidelity PCR using Phusion DNA Polymerases (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... and small intestinal organoids by Total RNA extraction reagent RNAiso Plus (TaKaRa Bio, Shiga, Japan) or NucleoSpin®RNA (MACHEREY-NAGEL ...
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bioRxiv - Cancer Biology 2022Quote: ... total RNA from all of the experimental groups was isolated using RNAiso Plus (Takara, Japan). Complementary DNA (cDNA ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 µg of total RNA was converted to cDNA using M-MLV reverse transcriptase (TAKARA) under standard conditions with oligo(dT ...
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bioRxiv - Cell Biology 2023Quote: ... Total RNA was purified from N2 using Nucleospin RNA®(Takara Bio Inc., Kusatsu, Japan) as described in manufacture’s procedure ...
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bioRxiv - Immunology 2024Quote: ... 500 ng total RNA or its tissues were then reverse-transcribed using PrimeScript RT (TAKARA) and a mixture of oligo-dT and random hexamer primers ...
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bioRxiv - Plant Biology 2024Quote: ... 2 μg of purified total RNA was treated with RNase-free DNaseI (Takara, Dalian, China) to remove contaminated genomic DNA ...
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bioRxiv - Neuroscience 2024Quote: Total RNA was extracted from tissues or cells using RNAiso Plus (Takara Biotechnology, Japan; 9109) according to the manufacturer’s guidelines ...
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bioRxiv - Biochemistry 2024Quote: ... DNaseI-treated total RNA (4 µg) was reverse transcribed using Primescript reverse transcriptase (Takara Bio) and random hexamers (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2020Quote: Y1H assay was performed using Matchmaker Gold Yeast One-Hybrid Library Screening System (Clontech, USA). The nucleotide sequences of promoter region of selected key defense genes having potential RAV1 binding motifs were retrieved by using online tool (https://bioinformatics.psb.ugent.be/plaza/) ...
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bioRxiv - Plant Biology 2021Quote: ... Yeast two hybrid assays were performed using the Matchmaker Gold Yeast Two-Hybrid System (Clontech). The standard protocol given in the kit was followed for Y2H assay ...
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bioRxiv - Plant Biology 2021Quote: ... β‐galactosidase assay was performed according to the manufacturer’s protocol (Matchmaker Two‐Hybrid System; Takara Bio ...
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bioRxiv - Microbiology 2020Quote: The yeast two-hybrid assays were performed in strain Saccharomyces cerevisiae Y2HGold (Takara Bio USA). Combination of the two plasmids encoding the desired fusion proteins were transformed into Y2HGold cells using Frozen-EZ Yeast Transformation II Kit (Zymo Research) ...
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bioRxiv - Genetics 2019Quote: The yeast two-hybrid assays were performed using the Matchmaker Yeast Two-Hybrid System (Clontech). Various fragments from OsLUGLWT and OsLUGLkkx were cloned into pGBKT7 ...
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bioRxiv - Plant Biology 2020Quote: Yeast two-hybrid assays were performed by the Matchmarker GAL4-based two-hybrid System (Clontech). Constructs were co-transformed into the yeast strain AH109 by the lithium acetate method (Gietz & Woods ...
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bioRxiv - Microbiology 2019Quote: The dual-luciferase assay using an NF-□B–dependent firefly luciferase (pNF-□B-Luc; Clontech) and a Renilla luciferase driven by the thymidine kinase promoter (pRLTK ...
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bioRxiv - Genetics 2022Quote: ... and then yeast two-hybrid assays were performed in a Gal4-based system from Clontech in a diploid strain by mating as previously described (Rossignol et al ...