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Citations for Takara Bio :
951 - 1000 of 2188 citations for Dengue Virus Serotype 4 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: Lenti-X 293T packaging cells (Clontech #11131D) were cultured in medium consisting of Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Cancer Biology 2024Quote: Lenti-X 293T cells (Takara, Cat # 632180) were transfected with each CAR expression plasmid and Mirus Bio™ TransIT™-Lenti Transfection Reagent (Cat # MIR6600) ...
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bioRxiv - Plant Biology 2024Quote: ... coli HST08 (Stellar competent cells, Takara Bio). Verified plasmids using Sanger sequencing were electroporated into A ...
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bioRxiv - Cancer Biology 2023Quote: ... plasmids to Lenti-X HEK293T cells (Takara) to generate lentiviral particles for transduction of the target cells as described in detail previously (Elegheert et al. ...
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bioRxiv - Cell Biology 2024Quote: ... Lenti-X™ 293T Cell Line (Takara) was cultured in 90% DMEM with high glucose (4.5 g/L) ...
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bioRxiv - Microbiology 2023Quote: iSLK cells and Lenti-X 293T (Takara) were maintained in DMEM (Gibco +glutamine ...
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bioRxiv - Developmental Biology 2021Quote: ... Sections were blocked in 5% goat serum then incubated with primary antibody at 4°C overnight (anti-dsRed for tdTomato (632496, 1:1000; Takara Bio, Mountain View, CA, USA), anti-SP7 (ab22552 ...
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bioRxiv - Microbiology 2020Quote: ... using each of the following 4 restriction enzymes: HaeIII or Hha I or Rsa I or Alu I (10 units, Takara Bio Co. Ltd. Shiga, Japan) in buffer solution (10xLow salt buffer ...
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bioRxiv - Microbiology 2022Quote: ... The open reading frame encoding each protein was amplified from RIB40 genomic DNA with PrimeSTAR® HS DNA Polymerase (TaKaRa Bio, Otsu, Japan) for high-fidelity PCR ...
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bioRxiv - Microbiology 2023Quote: The open reading frames of 19 LD-associated proteins were amplified from RIB40 genomic DNA with PrimeSTAR® HS DNA Polymerase (TaKaRa, Otsu, Japan) for high-fidelity PCR ...
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bioRxiv - Plant Biology 2022Quote: ... and impact of SAID1/SAID2 on SE interaction with other proteins was examined on SD-His/-Leu/-Met/-Trp quadruple dropout medium (Clontech, Cat. No. 630429) supplemented with 5 mM 3-amino-1,2,4-triazole (3-AT ...
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bioRxiv - Microbiology 2023Quote: The following plasmids were used in this study and previously described: enhanced green fluorescent protein (EGFP) pEGFP-N1 (Clontech Laboratories, Mountain View, CA), pCI-neo HSV-2 UL21 (UL21 ...
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bioRxiv - Cell Biology 2021Quote: ... HUVECs were maintained in Endothelial Cell Basal Medium 2 supplemented with Endothelial Cell Growth Medium kits (C22211, C22111, Takara). For replating cells ...
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bioRxiv - Developmental Biology 2023Quote: ... prior to cells being fully resuspended to a single-cell suspension in 2mL NDiff227 with a 1mL pipette (Takara). Using a multichannel pipette ...
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bioRxiv - Biophysics 2023Quote: ... All remaining unsorted cells in addition to the sorted cell populations were then miniprepped (Takara NucleoSpin Plasmid miniprep kit), and DNA was stored at 4 °C short-term or at −20 °C for long-term storage.
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bioRxiv - Cell Biology 2024Quote: ... 1×105 cells of HeLa cells or BJ-5ta cells expressing inducible TM-mNG21-10 were transfected with 7.5 pmol of Cas9 (TAKARA), 7.5 pmol of sgRNA ...
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bioRxiv - Cell Biology 2020Quote: ... Protein samples were run on a 4-12% Bis-Tris gel (Novex) and Western blots were performed with the following antibodies: α-GFP (Clontech Living Colors 632381 (JL-8); RRID:AB_2313808 ...
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bioRxiv - Cell Biology 2021Quote: Cell lysis was assessed by measuring Lactate Dehydrogenase (LDH) activity in cell supernatants using the LDH cytotoxicity detection kit (TaKaRa). To obtain the positive control ...
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bioRxiv - Cancer Biology 2020Quote: Whole Genome Amplification (WGA) was performed on 300 single cells for each Time by means of SMARTer PicoPLEX Single Cell WGA kit (Takara) following manufacturer’s protocol as previously described24 ...
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bioRxiv - Immunology 2022Quote: Lytic cell death was determined by measuring LDH release from cell-free supernatants using a colorimetric assay (Promega or Takara) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... Input RNA was equilibrated to a starting input cell number of 300 cells per population before cDNA and sequencing library production were performed using SMARTSeqv4 (Clontech) and NexteraXT (Illumina ...
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bioRxiv - Immunology 2019Quote: ... All cell lines were obtained directly from the ATCC (with exceptions of Lenti-X 293T cells, which were obtained from Clontech Laboratories ...
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bioRxiv - Cell Biology 2020Quote: To develop the EpH4 ES-FUCCI stable cell line we linearized the ES-FUCCI vector with AseI and transduced cells using Xfect (Clontech) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... The ZsGreen-ODC (ornithine decarboxylase) cells were previously generated by transfection of MelJuSo cells with the ZsProsensor-1 plasmid (BD Bioscience Clontech)20 ...
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bioRxiv - Cell Biology 2024Quote: ... iSLK and iSLK-BAC16 cell line derivatives were generated using 2nd generation lentivirus as follows: 1×106 Lenti-X 293T cells (Clonetech/Takara via the UC Berkeley Tissue Culture Facility ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The α1-subunit genes were inserted at the PH promoter of vectors already containing the corresponding β1-subunit proteins using In-Fusion® HD Cloning Kit (Takara Bio, USA Inc.) and control sequenced ...
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bioRxiv - Immunology 2023Quote: ... The 6-His tagged recombinant proteins were purified from the supernatant by gravity-fed through TALON® Metal Affinity Resin (Takara Bio, Shiga, Japan). Following a wash step with PBS (pH 8) ...
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bioRxiv - Cell Biology 2023Quote: ... crossed with WT BDF1 males at E0.5 and electroporated with RNP complex of Mavs targeting sgRNA (100 ng μL-1) and Cas9 protein (Takara Bio; 500 ng μL-1) using NEPA21 electroporator ...
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bioRxiv - Biochemistry 2023Quote: ... The PCR fragments of spike protein DNA were cloned into linearized pMRNAXP vector using In-Fusion® HD Cloning Kit (Clontech® Laboratories, Inc.). The cloning mixtures were transformed to One Shot™ TOP10 Chemically Competent E ...
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bioRxiv - Neuroscience 2023Quote: ... sagittal sections (50 µm) of the cerebellum were cut and incubated with antibodies against the reporter protein mCherry (Clontech 632543, RRID: AB_2307319, 1:500) and against the P-cell marker calbindin (Swant CB38 ...
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bioRxiv - Cancer Biology 2021Quote: ... HEK293T Lenti-X cells were purchased from Takara. Primary mouse melanoma cell lines were isolated from mouse melanomas ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were transfected using Xfect Transfection Reagent (Takara). Next ...
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bioRxiv - Biophysics 2022Quote: MCF7 Tet-off cells (Clontech, Cat No. 631154) were grown on Dulbecco Modified Eagle Medium (DMEM ...
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bioRxiv - Cell Biology 2022Quote: ... and Lenti-X 293T cells were from Clontech. No commonly misidentified cell line was used in this study ...
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bioRxiv - Cancer Biology 2019Quote: ... Lenti-X 293T cells were obtained from Clontech, and maintained in DMEM media (Hyclone or Gibco ...
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bioRxiv - Bioengineering 2019Quote: ... and transfected into Stellar Competent cells (Takara/Clontech). For each PCR amplicon ...
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bioRxiv - Bioengineering 2019Quote: ... and transfected into Stellar Competent cells (Takara/Clontech). For each PCR amplicon ...
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bioRxiv - Genomics 2021Quote: ... Transformation was performed with Stellar Competent Cells (Takara) which were thawed on ice ...
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bioRxiv - Neuroscience 2020Quote: ... Lenti-X 293T cells (Cat. No. 632180, Clontech) were plated on 5-10 cm dishes ...
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bioRxiv - Cancer Biology 2021Quote: ... Lenti-x-293 Cell Line (Clontech Laboratories, 632180) were cultured in Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Biochemistry 2020Quote: ... Lenti-X 293T cells (Takara Bio, MountainView, CA) were transiently transfected with 17.8 µg of pLKO.1 TRC-DsRed ...
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bioRxiv - Cancer Biology 2021Quote: ... Vectors were amplified in Stellar Competent Cells (Clontech). Successful shRNA integration was verified by Sanger sequencing (primer ...
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bioRxiv - Biochemistry 2021Quote: ... coli Stellar™ cells (Clontech, see reagent Table). Transformation and bacterial screening were carried out using standard cloning methods ...
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bioRxiv - Microbiology 2020Quote: ... For cell death measurement by LDH assay (Takara), culture supernatants were clarified with a 5 minute spin at 400×g ...
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bioRxiv - Bioengineering 2022Quote: ... Lenti-X 293T cells were purchased from Takara and cultured according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Lenti-X 293T cells were obtained from Clontech, and maintained in DMEM media (Hyclone or Gibco ...
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bioRxiv - Bioengineering 2022Quote: ... Lenti-X 293T cells were purchased from Takara and cultured according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: MCF7 Tet-off cells (Clontech, Cat No. 631154) were grown on Dulbecco Modified Eagle Medium (DMEM ...
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bioRxiv - Developmental Biology 2022Quote: ... were co-transfected into 293T cells (Clontech, 632180) with PEI reagent (Polysciences ...
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bioRxiv - Neuroscience 2021Quote: ... and transformed into competent Stellar Cells (Takara Bio). Recombinant plasmids were extracted using Roti-Prep Plasmid Mini (Carl Roth ...