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Citations for Takara Bio :
951 - 1000 of 5174 citations for Arg8 Vasopressin AVP ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: Sequence of the human variable heavy and kappa chains were obtained by using SMARTer 5’ RACE technology (Takara Bio USA) adapted for antibodies to amplify the variable genes from heavy and kappa chains for each hybridoma ...
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bioRxiv - Molecular Biology 2020Quote: ... The sgRNA target locus on exon 3 or 5 was PCR amplified with Terra™ PCR Direct Polymerase Mix (Takara) for 35 cycles using primer sets mEX3F and mEX3R or mEX5F and mEX5R (Table 1) ...
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bioRxiv - Molecular Biology 2022Quote: ... and the RNA was concentrated and reverse transcribed with a 5’-RACE protocol that appends a new primer site to the cDNA of both cleaved and uncleaved RNA (SMARTScribe, Takara). The cDNA was PCR amplified with primers that add the adaptors for Illumina sequencing ...
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bioRxiv - Physiology 2022Quote: ... the beads were well-washed by column binding buffer 5 times and then incubated in 2× Protein SDS PAGE Loading (Takara) 100°C for 5 min to completely elute the proteins ...
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bioRxiv - Systems Biology 2022Quote: ... Optimized coding sequences were synthesized as gBlocks (Integrated DNA Technologies) carrying 16-base pair overhangs at the 5’ and 3’ ends to facilitate in-fusion cloning (Clontech) into pET expression vectors (EMD MIllipore).
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bioRxiv - Immunology 2022Quote: ... 25 μg of the retroviral expression vector (pLZRS-human codon-optimized Bcl6-P2A-human codon-optimized BclxL-IRES-GFP) and 5 μg of the envelope vector (p10A1; Clontech) were transfected into GP2-293 cells using Lipofectamine 3000 (ThermoFisher ...
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bioRxiv - Microbiology 2019Quote: ... All ∼5 kb products were amplified from bat genomic DNA by inverse PCR employing the PrimeSTAR MAX DNA polymerase (Takara), purified by gel extraction (Axygen) ...
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bioRxiv - Genetics 2020Quote: ... The target vector and pEF1a-pac vector were co-transfected (5:1 ratio) to E14 mESCs using Xfect according to the manufacture’s instruction (TaKaRa, Inc). 48 hours after transfection ...
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bioRxiv - Molecular Biology 2020Quote: ... The FOXR1 wild-type and M280L mutant were then PCR amplified using forward 5′-AAAGCACTCGAGATGGGGAACGAGCTCTTTCTG-3’andreverse5’-TTTGGCCCGCGGTTAAAGATCAAAGAGGAAGGG-3’ primers and subcloned into the XhoI and SacII restriction sites of pEGFP-C3 (Clontech) to create an N-terminal EGFP tag ...
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bioRxiv - Molecular Biology 2021Quote: ... was then added to the supernatant to a final concentration of 5 mM and the solution was subjected to immobilized metal affinity chromatography (IMAC) by incubation with TALON cobalt resin (Takara) for 1 hr at 4°C ...
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bioRxiv - Biophysics 2020Quote: ... containing either tetra-acetylated or unmodified histone H4 was digested for 5 min at 22 °C with micrococcal nuclease (MNase) (0.125 to 2.0 units; Takara, cat. #2910A) in 5.5 mM Tris-HCl buffer (pH 7.6 ...
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bioRxiv - Genetics 2021Quote: ... The fragment length of PCR products was detected by 1.2% agarose gel electrophoresis using 5 μl of 100 bp DNA Ladder (TaKaRa, Japan) and gel stain (TransGen Biotech ...
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bioRxiv - Cell Biology 2020Quote: ... with 5’-cgGAATTCaccATGgagcagaagctc atctcagaagaagacctcggtAAGGATAACACCGTGCC-3’ and 5’-ataagaatGCGGCCGCtaaact ATTATTTTTCTGCACTACGCAGG-3’ followed by cloning into the EcoRI and Not I sites of pIRESpuro3 (Clontech) creating pIRESpuro3-myc-BirA ...
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bioRxiv - Immunology 2020Quote: ... cDNA was generated by template-switch reverse transcription according to the SMARTer RACE 5’/3’ manual using the SMARTScribe Reverse Transcriptase (Takara) with a template-switch oligo including an 18-nucleotide unique molecular identifier (UMI) ...
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bioRxiv - Immunology 2021Quote: ... 5′-GTGCATGCGGAAACACGTGTCTGG-3′ into pRRLU6-empty-gRNA-MND-Cas9-t2A-Puro vector or RNase L targeting gRNA 5′-GTTATCCTCGCAGCGATTGCGGGG-3′ into pRRLU6-empty-gRNA-MND-Cas9-t2A-Blast was achieved using the In-Fusion enzyme mix (Clontech). OAS1 and RNASEL KO 293T were generated using lentiviral transduction as described previously followed by selection in 2 μg/mL puromycin or blasticidin (Lau et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... PCR purified integrin β5 with engineered flanking restriction sites (Xho-I/BamHI) was subcloned into the multi-cloning sites of pEGFP-N1 (Clontech) to encode an in-frame fusion protein with the carboxy-terminal EGFP-tag (pEGFP-N1-Integrin β5 ...
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bioRxiv - Genomics 2022Quote: Genomic DNA samples (5 ng) were used as templates in 20 μl reactions performed with PrimeSTAR GXL DNA Polymerase (TaKaRa), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Transferred membranes were blocked with 5% (w/v) milk for 30 mins at RT before being hybridized with the corresponding anti-GFP (632381, Takara) or anti-mCherry (632543 ...
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bioRxiv - Immunology 2022Quote: ... Lentiviral transduction of murine alveolar macrophages was performed for 7 days in the presence of 5 μg/cm2 RetroNectin (Takara). Stable gene expression was confirmed by GFP signals using a BZ-X710 (KEYENCE ...
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bioRxiv - Molecular Biology 2022Quote: ... The cDNAs (1-5 ng RNA equivalents) were used for real-time PCR amplification using SYBR Premix Ex Taq II (Takara/Clontech) on a 7500 Fast Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2022Quote: ... the NaCl concentration of the cleared lysate was adjusted to 500 mM and the cleared lysate was applied to 10 mL (=5 mL bed volume) equilibrated Talon SuperFlow Metal Affinity Resin (TaKaRa) per L culture on ice for 45 min ...
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bioRxiv - Bioengineering 2022Quote: ... The solution was heated at 65°C for 5 minutes using Takara® PCR Thermal Cycler (Takara Corp., Shiga, Japan), and then gradually cooled down to 30°C over a time span of 10 minutes before settling down to 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... iBMDMs were seeded into 24-well plates (5×104 cells/well) and transfected with 600 ng and 1200 ng of the eukaryotic expression vector (pCMV-HA; Clontech) harbouring TcpB for 24-hours ...
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bioRxiv - Cell Biology 2023Quote: ... Up to 5 μl of assembly reactions were used for heat-shock transformation of Stellar™ Competent Cells (Takara Bio).
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bioRxiv - Immunology 2022Quote: ... cDNA was generated from 10 μl RNA according to the SMARTer RACE 5’/3’ manual using SMARTScribe Reverse Transcriptase (Takara) and a self-designed template-switch oligo (AGGGCAGTCAGTCGCAGNNNNWSNNNNWSNNNNWSGCrGrGrG) ...
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bioRxiv - Plant Biology 2023Quote: ... diluted to 1 µg/mL with Milli-Q ultra-pure water for 5 min at room temperature or with a pollen germination medium containing 2000-fold SYBR Green I (Cat#: 5760A, Takara) and 5 µg/mL DAPI (Cat# 10236276001 ...
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bioRxiv - Microbiology 2023Quote: Sequence of the mouse variable heavy and kappa chains were obtained by using SMARTer 5’ RACE technology (Takara Bio, USA) adapted for antibodies to amplify the variable genes from heavy and kappa chains for each hybridoma based on isotype ...
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bioRxiv - Evolutionary Biology 2022Quote: A yeast two-hybrid prey cDNA library of Trichoplax whole animal RNA was prepared in the pGADT7- AD plasmid vector using the Make Your Own “Mate & Plate™” Library System (Takara Bio, California USA) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... Positive blue colonies were streaked to an -Ade-His-Leu-Trp dropout selective media agar plates supplemented with Aureoblastidin A and X-gal (Clontech yeast two-hybrid manual).
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bioRxiv - Molecular Biology 2023Quote: ... tabaci adults was used for the synthesis of first strand cDNA utilizing the make your own mate & plate library system with CDS III adaptor primer and smart III oligo (template switching) as per manufacturer’s instructions (Takara Bio, San Jose, CA, USA). The first strand cDNA was used as template for long distance PCR with 5’ and 3’ primers ...
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bioRxiv - Cancer Biology 2021Quote: RNA was extracted using the nucleospin RNA kit (Takara) according to the manufacturer’s recommended protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... SMART-Seq v4 Ultra Low input RNA kit (Clontech) was used to amplify cDNA which was subsequently sheared resulting in 200-500 bp fragments ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA was generated using PrimeScript RT kit (Takara #RR037A). For semi-quantitative RT-PCR ...
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bioRxiv - Molecular Biology 2021Quote: ... using a Random Primer DNA Labeling Kit (TaKaRa, 6045) according to the manufacturer’s protocol ...
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Inositol depletion regulates phospholipid metabolism and activates stress signaling in HEK293T cellsbioRxiv - Cell Biology 2022Quote: ... coli using the NucleoSpin Plasmid miniprep kit (Takara 740588). Transformants were sequenced to confirm successful cloning ...
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bioRxiv - Immunology 2021Quote: ... The SMARTer ThruPLEX DNA-Seq kit (Takara, Rockville, MD) was utilized to generate libraries from 10 μl of sheared cDNA template ...
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bioRxiv - Molecular Biology 2021Quote: ... using the In-Fusion® HD Cloning Kit (Takara). The subsequent mutations in the TCRA and TCRB 3’-UTRs were generated using these initial constructs ...
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bioRxiv - Molecular Biology 2021Quote: ... and CalPhos transfection kit according to manufacturer’s instructions (Takara). 2 days after transfection ...
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bioRxiv - Cell Biology 2019Quote: ... using the IN-Fusion HD EcoDry Cloning Kit (Clontech).
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bioRxiv - Biochemistry 2019Quote: ... A SYBR Premix Ex Taq kit (TaKaRa, Dalian, China) was used to perform quantitative PCR on a CFX96 Real-Time PCR detection system (BioRad) ...
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bioRxiv - Physiology 2020Quote: ... and titered with Adeno-X Rapid Titer Kit (Clontech). Adenoviruses were delivered into mice intravenously at 5 × 108 pfu/mouse for overexpression experiments ...
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bioRxiv - Systems Biology 2019Quote: ... using the In-Fusion® HD Cloning Kit (Clontech) and included a 12 residues linker in between (Linker 2 ...
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bioRxiv - Zoology 2020Quote: ... MiniBEST Universal RNA Extraction Kit was purchased from TaKaRA, iScript™ gDNA Clear Synthesis Kit was purchased from Bio-Rad.
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bioRxiv - Developmental Biology 2020Quote: ... using the In-Fusion HD Cloning Kit (Takara, 638909).
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bioRxiv - Evolutionary Biology 2020Quote: ... In-Fusion HD cloning kit was purchased from Takara. All the experiments were performed using ultrapure water purification system from a MilliQ Integral MT10 type 1 (Millipore).
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bioRxiv - Developmental Biology 2021Quote: ... the SMARTer® Ultra® Low RNA Kit (Takara) was used for library preparation ...
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bioRxiv - Biochemistry 2021Quote: ... Lenti-X p24 rapid titre kit (Takara Bio, JP) was used to quantify the viral titres ...
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bioRxiv - Bioengineering 2020Quote: ... Yeast transformation by Fast Yeast TransformationTM kit (Takara Bio) used for integrated linear pAUR101 vector and its associated genes in AUR1-C locus ...
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bioRxiv - Neuroscience 2020Quote: ... Library preparation was performed using the SMARTer kit (Clontech), and sequencing performed on an Illumina HiSeq3000.
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bioRxiv - Neuroscience 2020Quote: ... cDNA was generated using Clontech SMARTer cDNA kit (Clontech Laboratories ...