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Citations for Takara Bio :
951 - 1000 of 2119 citations for 7 Benzyloxy 10 11 dihydrodibenzo b f 1 4 thiazepin 11 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... For STEM121 (1:5000, Takara, #Y40410) and Ki67 (1:100 ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μg/ml of puromycin (Takara) was added to the medium only for two days.
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bioRxiv - Genomics 2023Quote: ... 1 Unit ExTaq (Takara, catalog #RR001A), 0.2 µM primer 1 ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 μg/ml Doxycycline (Dox) (Clontech) was added into PGC-cultured FAcs medium 24 hours before the injection ...
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bioRxiv - Molecular Biology 2023Quote: ... His6 (631212, Clontech, mouse, 1:500); NPL4 (sc-365796 ...
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bioRxiv - Cell Biology 2023Quote: ... rabbit anti-Myc (1:1000) (Clontech); rabbit anti-HA (1:800 ...
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bioRxiv - Developmental Biology 2023Quote: ... DsRed (Clontech, cat#632392, 1:500), GFP (Thermo ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit anti-DsRed (1:1000, Clontech), mAb anti-Bruchpilot (nc82 ...
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bioRxiv - Cell Biology 2023Quote: HEK293 (Clontech #C3003-1; lot #7030396) cell lines were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) ...
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bioRxiv - Bioengineering 2023Quote: ... and Stem123 (Takara Y40420; 1:500). The following secondary antibodies were used at a 1:800 dilution ...
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bioRxiv - Developmental Biology 2023Quote: ... anti-Ocn (Takara, M173; 1:800), anti-CD31 (BD ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µL of RNaseH (TaKaRa, 2150A), and 1 µL of RNase cocktail (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... GFP (1:1,000, JL-8, Clontech), GABARAP/Atg8a (1:2,000 ...
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bioRxiv - Cell Biology 2023Quote: ... mouse anti-GFP (Clontech,1:6,000), mouse anti-FLAG (Millipore-Sigma ...
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Targeted degradation of PCNA outperforms stoichiometric inhibition to result in programed cell deathbioRxiv - Cell Biology 2022Quote: Human embryonic kidney T-REx 293 cells which express the tetracycline repressor protein were purchased from Thermo Fisher Scientific and cultured in Eagle’s Minimum Essential Medium (MEM) GlutaMAX™ supplemented with 10% Tet system approved FBS (Clontech) and 5 μg/mL blasticidin ...
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bioRxiv - Developmental Biology 2022Quote: ... https://www.addgene.org/112849/)10 between the EcoRI and SpeI restriction sites by multi-cassette assembly using the InFusion cloning kit (Takara Inc.). The correct sequence of the donor region was validated by Sanger sequencing.
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bioRxiv - Molecular Biology 2021Quote: 20 cm plates of HEK293T cells were transfected with 10 µg of each plasmid using CalPhos transfection kit (Takara) according to manufacturer’s instructions and harvested 3 days later ...
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bioRxiv - Molecular Biology 2020Quote: ... where each well contained 20 μL of reaction mixture consisting of: 10 μL SYBR Premix Ex Taq II (TaKaRa), 0.4 μL ROX Reference Dye (50× ...
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bioRxiv - Immunology 2019Quote: ... Non-tissue culture treated 24-well plates were coated with 0.5 mL per well of 10 μg/mL RetroNectin (Takara) on day 1 and stored overnight at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... The PCR reaction mixture (10 μl total volume) included 5 μl SYBR Premix Ex Taq II (TaKaRa, Dalian, China), 3.5 μl ddH2O ...
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bioRxiv - Genomics 2021Quote: ... and then resuspended in 10 mM TAPS pH8.5 containing 1X DAPI and 1X secondary diluent reagent (Takara Cat# 640196) at a concentration of 400 nuclei/µL ...
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bioRxiv - Molecular Biology 2021Quote: DIE cells were cultured in growth medium consisting of IMDM basal medium with 10% Tet system-approved FBS (Clontech) and 55μM 2-mercaptoethanol ...
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bioRxiv - Cancer Biology 2020Quote: ... full-length Bcor cDNA or truncated BcorΔE9-10 cDNA was cloned with an N-terminal HA tag into pCAG vector using InFusion (Takara). pCMV-SPORT 6.1 with mouse Bcl6 cDNA was purchased from Horizon Discovery (Clone ID 6309948).
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bioRxiv - Neuroscience 2021Quote: ... 10 μM ROCK inhibitor (Y-27632; Selleckchem, Cat. No. S1049) and 2 μg/mL doxycycline (Clontech, Cat. No. 631311). Media was changed daily during this stage.
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bioRxiv - Neuroscience 2019Quote: ... Each reaction was performed in triplicate in 10 μL volume containing 1X SYBR Premix Ex Taq II (Takara Biotechnology), 50nM of each primer and 100ng of cDNA ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were maintained at 37 °C in a humidified atmosphere at 5% CO2 in DMEM 4.5g/L Glucose with UltraGlutamine media supplemented with 10% of Tet-free FBS (Clontech) and 1% penicillin/streptomycin.
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bioRxiv - Immunology 2019Quote: ... Retroviral supernatant (5 ml) was added to non-treated retronectin-coated (10 µg/ml) 6-well plates (Takara Bio) and incubated for 4 h at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... All qPCR reactions were performed as 10 μl reactions using TB Green™ Premix Ex Taq™ II (TaKaRa). Primers for qPCR were listed in Table S2 ...
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bioRxiv - Microbiology 2020Quote: ... thlaspeos tissue was resuspended in 9 ml protoplasting buffer supplemented with 10 mg/ml Yatalase (Takara Bio, Kusatsu, Japan) and 20 mg/ml Glucanex (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... the pellet was re-suspended in four millilitres of filtered-sterilized (0.22 µm filter) lysing buffer (75 mg/mL BSA; 10 mg/mL Yatalase (Takara); 5 mg/mL Lysing Enzymes (L1412 Sigma) ...
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bioRxiv - Immunology 2021Quote: ... bone marrow was harvested and transduced with SINV virus on plates coated with 10 μg/cm2 Retronectin (T100, Takara) for 1.5-2 hrs at 650×g and 37°C ...
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bioRxiv - Plant Biology 2022Quote: ... PCR reaction mixture was prepared at a final volume of 20µl which contains 10 µl of TB Green Premix Ex Taq II (Takara), 10pM of forward and reverse primers and 2µl of diluted cDNA ...
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bioRxiv - Microbiology 2022Quote: ... 10 μl of the reaction mixture was prepared with 5 μl of SapphireAmp Fast PCR Master Mix (Takara #RR350B), 1 μl of each of forward and reverse primers (final concentration 0.2 μM) ...
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bioRxiv - Microbiology 2022Quote: ... single-cells were isolated using a BD FACS Aria III for sorting individual cells into 96-well plates prefilled with lysis buffer (0.26 μl of 10×Lysis buffer (Takara), 0.03 μl of RNase Inhibitor (100 U/μl ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Supernatant containing virus was collected 48 h later and 10-X concentrated using Lenti-X Concentrator (Takara Bio, Germany). AML-12 wild-type cells were transduced using concentrated virus and selected using 3 μg/mL of puromycin ...
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bioRxiv - Cell Biology 2022Quote: ... Reverse transcription was performed with 10 ng RNA using the SMART-Seq v4 Ultra Low Input RNA Kit (Takara) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... GP2-293 cells were cultured in DMEM (FUJIFILM Wako Pure Chemical) supplemented with 10% tetracycline-free FBS (Takara Bio).
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bioRxiv - Microbiology 2022Quote: ... Isolated RNA or serial ten-fold dilutions of RNA standards for the ORF1ab amplicon (ranging from 2.25×10^6 to 250 copies/rxn) were reverse transcribed using the Takara Prime Script RT kit (Takara) using poly(A ...
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bioRxiv - Microbiology 2023Quote: ... RT-PCR was performed using 500 ng of RNA in 10 µl volume of Solution with PrimeScript RT (TAKARA) and random hexamer primers ...
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bioRxiv - Synthetic Biology 2023Quote: A 10 cm dish of HEK293T cells that had been transfected with the GFP expressing plasmid pEGFP-N1 (Clontech) was washed with PBS and lysed by sonication on ice in PBS supplemented with 0.2 % Triton X100 ...
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bioRxiv - Cell Biology 2024Quote: ... 1×105 cells of HeLa cells or BJ-5ta cells expressing inducible TM-mNG21-10 were transfected with 7.5 pmol of Cas9 (TAKARA), 7.5 pmol of sgRNA ...
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bioRxiv - Cell Biology 2020Quote: ... Protein samples were run on a 4-12% Bis-Tris gel (Novex) and Western blots were performed with the following antibodies: α-GFP (Clontech Living Colors 632381 (JL-8); RRID:AB_2313808 ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated in primary antibody (1:1,000 mouse anti-calbindin, Sigma; 1:500 rabbit anti-DsRed, Clontech; 1:1,000 guinea pig anti-vGluT1 ...
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bioRxiv - Developmental Biology 2023Quote: ... primary antibodies (chicken anti-GFP, Abcam, ab13970, 1:2000 and rabbit anti-DsRed, Clontech, 632496, 1:200) were incubated overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... 1-st PCR (PCR 1) was performed using Titanium Taq DNA Polymerase (# 639209, Takara Bio, CA, USA). Separation of the PCR products from primers and gel purification was done by QIAquick PCR & Gel Cleanup Kit (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... pLKO.1-shFEN1 or pLKO.1 control vector was simultaneously transfected into LentiX293T (Cat# 632180 Clontech, Japan) with packaging plasmid ...
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bioRxiv - Molecular Biology 2024Quote: ... 4.9 μg DNA was transfected per dish containing a 1:1 molar ratio of pmCherry-N1 (Clontech) and of the pUC18-GFP or linear GFP substrate ...
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bioRxiv - Molecular Biology 2021Quote: Slide embedded tissue sections were incubated in PBS containing 0.2 mg/ml saponin (Nacalai teques) and 10 μg/ml Proteinase K (Takara Bio) for 20 min at RT ...
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bioRxiv - Molecular Biology 2021Quote: TET-ON stable HEK293 cells with the Tetracycline-inducible expression constructs were grown in DMEM supplemented with 10% TET-approved FCS (Clontech) and induction of expression of respective gene product was carried out for indicated time durations using 400 ng/ml Doxycycline (SIGMA) ...
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bioRxiv - Cancer Biology 2019Quote: ... we performed 30 separate 100 μL PCR reactions with 10 μg genomic DNA in each reaction using ExTaq DNA Polymerase (Clontech) then combined the resultant amplicons ...