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Citations for Takara Bio :
51 - 100 of 1086 citations for TRAILR 2 TNFRSF10B Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... AmphoPack-293 cells (Takara Bio Inc., CVCL_WI47) were transfected with pMSCV-FLuc-T2A-GLuc-Hygro using calcium phosphate protocol69 ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1.25Lμg pVSV-G (second-generation lentivirus packaging system) into human embryonic kidney packaging cells GP2-293 (Clontech, Inc., Mountain View, CA, USA), using CalPhos™ Mammalian Transfection Kit (Clontech ...
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bioRxiv - Neuroscience 2020Quote: HEK 293T (Lenti-X 293T subclone, TaKaRa) were maintained in DMEM ...
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bioRxiv - Immunology 2021Quote: HEK 293T or Lenti-X 293T (Takara) were seeded in complete DMEM in 6-well plate to reach 60–80% confluency after one day ...
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bioRxiv - Immunology 2020Quote: HEK 293T or Lenti-X 293T (Takara) were seeded in complete DMEM in 6-well plate to reach 60–80 % confluency after one day ...
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bioRxiv - Microbiology 2020Quote: HEK-293T (Lenti-X 293T, Takara Bio) and Vero E6 (ATCC ...
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bioRxiv - Molecular Biology 2023Quote: ... Lenti-X HEK 293T cells (Takara Bio) were transfected with lentiviral packaging plasmids and transgenic constructs ...
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bioRxiv - Molecular Biology 2022Quote: ... anti-His (Clontech 631212), anti-H3K36me2 (Upstate 07-369) ...
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bioRxiv - Cancer Biology 2021Quote: ... Lenti-x-293 Cell Line (Clontech Laboratories, 632180) were cultured in Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Microbiology 2022Quote: Retrovirus packaging cell line GP2-293 (Takara Bio) expressing Moloney murine leukemia virus gag and pol genes was co-transfected with pLNCX2 vector with the FLAG-APEX2-GARG1060 insert and a plasmid coding for the vesicular stomatitis virus envelope glycoprotein (Takara Bio ...
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bioRxiv - Molecular Biology 2024Quote: ... The N-terminal His tag construct of human MPST was co-transformed with GroES-EL chaperon plasmid from Takara (#3340), overexpressed in E ...
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bioRxiv - Microbiology 2023Quote: ... His-tagged mCherry (mCherry-His) was PCR amplified from pmCherry-C1 vector (Takara) templates with primers encoding a His-tag and NcoI/NotI cut sites (Takara) ...
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bioRxiv - Microbiology 2021Quote: ... and HEK 293T Lenti-X (Clontech/Takara Bio) were cultured at 37°C in 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Microbiology 2021Quote: ... and HEK 293T Lenti-X (Clontech/Takara Bio) were cultured at 37°C in 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Cancer Biology 2019Quote: ... Lentivirus was produced using HEK-293T cells (Clontech) and the overexpression vector was confirmed in the cells by GFP fluorescence and western blot (Figure 2A-B ...
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bioRxiv - Cell Biology 2021Quote: ... HEK 293T cells (Takara Bio Inc. Cat#: 632180) were cultured in DMEM (Gibco Cat# ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... HEK 293T cells (Clontech, Mountain View, CA, USA) were grown in 24 well dish to 50%– 60% confluency ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: HEK 293T cells (Clontech, Mountain View, CA, USA) were grown in 15 cm dish to 60%–70% confluency ...
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bioRxiv - Neuroscience 2022Quote: ... and the AAVpro 293 T cell line (#632273, Takara) following the manufacturers’ instructions ...
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bioRxiv - Immunology 2024Quote: ... genes encoding the variable regions of C7 and C74 heavy chains were cloned into a pMN vector with a human CH1 domain and a C-terminal His-tag using In-Fusion cloning system (Takara Bio #639649). The full light chains of C7 and C74 were cloned into the pMN vector without any purification tag using the same method ...
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bioRxiv - Developmental Biology 2023Quote: ... Interactions were evaluated on agar that was -His or -His -Ade (Clontech, now Takara Bio). Growth in the absence of either supplement indicates transcription from reconstituted GAL4 proteins ...
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bioRxiv - Developmental Biology 2023Quote: ... Interactions were evaluated on agar that was -His or -His -Ade (Clontech, now Takara Bio). Growth in the absence of either supplement indicates transcription from reconstituted GAL4 proteins ...
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bioRxiv - Biophysics 2022Quote: ... HEK-D (see below) and Lenti-X 293T (Takara) cells were cultured in growth medium consisting of Dulbecco’s modified Eagle’s medium (GIBCO) ...
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bioRxiv - Cell Biology 2022Quote: ... HEK-293T cells were transfected with calcium phosphate (Clontech) in T75 flasks using a total of 30 μg plasmid DNA ...
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bioRxiv - Physiology 2020Quote: ... HEK cells were transiently cotransfected with pEGFP-N1 (Clontech), and either drSCN4Ab ...
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bioRxiv - Immunology 2023Quote: ... HEK 293T cells (RRID:CVCL_0063; Lenti-X 293T cells, Takara) were maintained in DMEM complete medium ...
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bioRxiv - Immunology 2021Quote: ... His-tagged NTD domain constructs were purified from clarified supernatants using 2 ml of cobalt resin (Takara Bio TALON), washing with 50 column volumes of 20 mM HEPES-HCl pH 8.0 and 150 mM NaCl and eluted with 600 mM imidazole ...
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bioRxiv - Cancer Biology 2023Quote: ... His-TEV tagged protein was captured with Co-TALON resin (Clonetech, Takara Bio USA, 2 mL slurry/liter culture) at 4 ºC for 1 h with constant end-to-end mixing ...
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bioRxiv - Plant Biology 2023Quote: ... Approximately 2.6 x 106 yeast transformants were screened on 2% SD/Gal/Raf/X-P-gal (-Ura/-His/-Trp/-Leu) following the manufacturer’s instructions (Clontech). Direct protein-protein interaction was confirmed by co-transformation of the respective plasmids into the yeast strain AH109 using the Matchmaker GAL4 Two-hybrid System (Clontech) ...
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bioRxiv - Cell Biology 2022Quote: ... and kidney 293 GP cells (Clontech, Mountain View, CA, USA) were cultured in Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Cell Biology 2020Quote: ... The GP2-293 cell line (Takara Bio Inc., Shiga, Japan) was used to produce the lentivirus by co-transfection with pVSVg ...
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bioRxiv - Immunology 2021Quote: ... The retroviral packaging line 293-GP was purchased from Takara Bio ...
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bioRxiv - Immunology 2023Quote: ... HEK293T (ATCC collection) and GP2–293 packaging cell lines (Clontech) were cultured in DMEM (10938-025 ...
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bioRxiv - Microbiology 2020Quote: ... CloneAmp Hi-Fi PCR Premix (Takara) and the following PCR conditions were used to generate the amplicons ...
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bioRxiv - Cell Biology 2021Quote: α-His primary antibody (1/10,000; Clontech) and HRP-conjugated goat anti-mouse IgG (H+L ...
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bioRxiv - Cell Biology 2020Quote: ... His (Takara/Clontech 631212, 1:1000), GFP (Roche 11814460001 ...
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bioRxiv - Cell Biology 2020Quote: ... His (Takara/Clontech 631212, 1:1000), GFP (Roche 11814460001 ...
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bioRxiv - Immunology 2021Quote: ... to transiently transfect HEK 293T Lenti-X cells (Takara/Clontech) to make lentiviral particles ...
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bioRxiv - Immunology 2021Quote: ... to transiently transfect HEK 293T Lenti-X cells (Takara/Clontech) to make lentiviral particles ...
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bioRxiv - Microbiology 2020Quote: HEK 293T Lenti-X cells (Clontech Laboratories, Mountain View, CA) were maintained in Dulbecco’s Modified Eagles medium (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... medium and 10-fold dilutions of these cultures were dropped on SD control (−2) and selective medium additionally lacking His (−3) (Clontech). Empty vectors were used as negative controls ...
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bioRxiv - Molecular Biology 2019Quote: ... These vectors were transfected in AmphoPack-293 cell line (631505, Clontech). Viral supernatants were harvested 48 h post transfection ...
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bioRxiv - Cell Biology 2020Quote: ... retroviruses were produced using the GP2-293 packaging cell line (Clontech), where pRetroX-TetOne puro empty vector (EV) ...
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bioRxiv - Microbiology 2023Quote: ... and pMD2.G using TransIT-293 Transfection Reagent (TaKaRa, Cat# V2700) in Opti-MEM I Reduced Serum Medium (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... to Lenti-XTM 293 cells (Takara Bio, San Jose, CA, USA) with X-tremeGENE HP (Roche Diagnostics ...
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bioRxiv - Microbiology 2024Quote: ... Transfection was performed using 4 µL TransIT 293 (Takara, Shiga, Japan) and 100 µL OPTI-MEM (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... Primer sequences (Table 2) were verified using total human kidney RNA (Takara Bio). PSMB4 was determined as the most stable housekeeping gene using the method described by Xie ...
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bioRxiv - Bioengineering 2019Quote: ... SD-His or SD-Trp-Ura (Clontech). Mycoplasma pneumoniae strain M129 (ATCC 29342) ...
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bioRxiv - Cancer Biology 2020Quote: ... HEK-LentiX cell line for lentivirus production was purchased from Takara Bio ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... To produce the viruses HEK Lenti-X 293T cell line (Clontech) were transfected using PEI (Sigma ...