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Citations for Takara Bio :
51 - 100 of 2443 citations for T cell leukemia lymphoma protein 1B TCL1B Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... and inserted to pMD19-T (TAKARA) to obtain pMD19-T-MMP0852/0853 (Table S1) ...
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bioRxiv - Molecular Biology 2020Quote: ... the amplified PCR products were cloned into a T/A cloning vector PMD18-T (TaKaRa, Tokyo, Japan), and later excised from PMD18-T plasmid using NotI and XhoI restriction sites ...
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bioRxiv - Plant Biology 2020Quote: ... YFP-CESA6 protein was detected using anti-GFP antibody (Takara, catalog # 632381) and SEC12 was detected using anti-SEC12 antibody (Bar-Peled and Raikhel ...
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bioRxiv - Plant Biology 2022Quote: ... CITRINE-fusion proteins were detected with anti-GFP antibody (JL-8, Takara Bio Clontech ...
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bioRxiv - Cancer Biology 2022Quote: ... Retroviruses corresponding to different CAR constructs were transduced into activated T cells in retronectin-coated plates (Takara). Cultures were allowed to expand for 10 days in their media supplemented with 300 IU ml-1 IL-2 ...
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bioRxiv - Cancer Biology 2024Quote: ... Activated T cells were transduced with the retroviral supernatant using retronectin-coated plates (Takara Bio Inc, T100B). T cells were harvested after three days and expanded in complete medium (45% RPMI-1640 (Corning ...
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bioRxiv - Molecular Biology 2024Quote: Human Embryonic Kidney cells stably expressing the SV40 large T antigen (HEK293T, ATCC) and GP2-293 (Clontech) cells were cultured in complete DMEM (10% FBS ...
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bioRxiv - Cancer Biology 2020Quote: ... was cloned into pMD19-T simple (TAKARA) to obtain pMD19-T-DMP firstly ...
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bioRxiv - Cell Biology 2021Quote: ... cloned into T-Vector pMD20 (Takara, 3270), and sequenced (Thermo ...
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bioRxiv - Developmental Biology 2020Quote: ... we used jetPrime (Polyplus) according to the manufacturer’s instructions to transfect Lenti-X 293 T HEK cells (Takara) with lentiviral production plasmids pMD2.G and psPAX2 along with our individual transfer plasmids ...
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bioRxiv - Synthetic Biology 2022Quote: Genomic DNA was extracted from sorted T cells using the Macherey-Nagel NucleoSpin Tissue XS kit (Takara #740901.250). DNA encoding the CAR costimulatory domain was amplified from the extracted genomic DNA using the forward primer 5’-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNNNNNACTGGTTATCACCCTTTA CTGC-3’ (Integrated DNA Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... Retroviral transduction was achieved by spinoculation of 3 × 106 mouse T cells on retronectin-coated plates (Takara Bio) with neat retroviral supernatant harvested from 293T packaging cells (2000xg ...
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bioRxiv - Bioengineering 2023Quote: ... T cells were mixed with lentivirus at multiplicity of infection (MOI) equal to 5 in Retronectin (Takara, T100B)-coated culture plates and centrifuged at 1800 g for 1 h at 32 °C for lentiviral transduction before returning to normal culture condition ...
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bioRxiv - Biophysics 2023Quote: ... The virus of each construct was produced in Lenti-X™ 293 T Cell Line (Takara, Cat. #632180) and previously described74 ...
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bioRxiv - Immunology 2024Quote: ... activated CD8+ T cells were transduced with retrovirus expressing B7-H3-CAR on retronectin-coated (cat. T100B, Takara) non-tissue-culture-treated plate in complete RPMI medium supplemented with 50 U/ml rhIL-2 ...
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bioRxiv - Cancer Biology 2024Quote: ... T cells were transduced using lentivirus at an MOI between 5 and 10 on Retronectin-coated (Takara, T100B) plates overnight to express an M5 or SS1 CAR ...
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bioRxiv - Molecular Biology 2023Quote: ... and 5 μg of total RNAs were reverse-transcribed into cDNA at 42°C for 1 h in 20 μl reaction mixture containing mouse Moloney leukemia virus reverse transcriptase (PrimeScript, TAKARA BIO, Shiga, Japan) with random 6 primers (TAKARA BIO) ...
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bioRxiv - Microbiology 2020Quote: ... Protein concentrations of cell lysates were measured using a bicinchoninic acid protein assay kit (Takara Bio Inc.). Cell lysate containing the same amount of protein was mixed with 10 volumes of methanol and then centrifuged at 20,000 × g for 30 min to precipitate proteins ...
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bioRxiv - Cell Biology 2021Quote: ... and inserted into T-Vector pMD20 (Takara Bio) using DNA Ligation Kit
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bioRxiv - Plant Biology 2024Quote: ... The pMD18-T vector was procured from TaKaRa Bioengineering (Dalian ...
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bioRxiv - Microbiology 2022Quote: ... and cloned into pMD18-T vector (TaKaRa, China) and the recombinant plasmids were picked and sent to Tsingke Biotechnology Co. ...
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bioRxiv - Plant Biology 2023Quote: ... 0.05% T-20 and protease inhibitor cocktail (Takara)] ...
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bioRxiv - Immunology 2021Quote: ... Such isolated naïve CD8 T cells were cultured for 3d prior transducton with 3rd generation lentiviral vectors on RetroNectin (Takara) coated cell culture plates (according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... GST and GST-fused proteins were transfected into the cells using Xfect™ Protein Transfection Reagent (TaKaRa, 631324) following the manufacturer’s instruction.
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bioRxiv - Biochemistry 2021Quote: ... monoclonal antibody for green fluorescent protein (JL-8; Takara Bio, Kusatsu, Japan; recognizes VC), rabbit anti-GFP (D5.1 Cell Signaling Technology 2956S ...
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bioRxiv - Plant Biology 2022Quote: ... CITRINE-fusion proteins were detected with anti-GFP antibody (JL-8, Takara Bio Clontech, dilution ...
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bioRxiv - Plant Biology 2021Quote: ... GFP-tagged proteins were detected using the anti-GFP antibody (JL-8, Clontech Takara) diluted 1:2000 (v/v ...
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bioRxiv - Plant Biology 2021Quote: ... GFP-tagged proteins were detected using the anti-GFP antibody (JL-8, Clontech Takara) diluted 1:2000 (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... GFP fusion proteins were revealed with mouse monoclonal antibody anti-GFP (JL-8, Clontech) used at 1/2000 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... Expression from the CMV-TetO2 promoter in Flp-In T-REx cells was induced by treatment with 10 ng/mL doxycycline (Clontech) for 24 hours ...
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bioRxiv - Cell Biology 2022Quote: ... E.coli competent cells transformed with pTf16 chaperone protein tag (TaKaRa Bio) per the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2020Quote: ... The cloning vector pMD20-T was purchased from Takara Bio ...
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bioRxiv - Microbiology 2020Quote: ... and cloned into T-vector pMD20 (Takara Bio Inc.). To amplify Vero-PVR1 ...
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bioRxiv - Cell Biology 2022Quote: mRNA was captured using poly-T coated dishes (Clontech) and used for library construction using SMARTer technology (SMARTer Stranded RNA-seq Kit ...
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bioRxiv - Cell Biology 2022Quote: ... mRNA was captured using poly-T coated dishes (Clontech) and SMARTer technology was used for library construction (SMARTer Stranded RNA-seq Kit).
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bioRxiv - Cell Biology 2023Quote: ... Clone and sequence using the pMD18-T vector (TaKara).
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bioRxiv - Microbiology 2024Quote: ... Yeast clones containing pGBKT7-53 and pGADT7-T (Clontech) were used as positive controls ...
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bioRxiv - Cell Biology 2023Quote: ... pH 7.4) and the protein contents of the cell lysate were quantified using a BCA Protein Assay Kit (TAKARA, T9300A) following the manufacturer’s protocol (Song et al. ...
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bioRxiv - Cancer Biology 2021Quote: ... Retroviruses encoding a second-generation CAR targeting HER-2 was obtained from the supernatant of the GP+E86 packaging line spun together with T cells onto RetroNectin-coated (10 μg/ml) 6-well plates (Takara Bio) and incubated overnight before the second viral transduction ...
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bioRxiv - Neuroscience 2021Quote: ... coated T-225 flasks containing 85-95% confluent HEK-293T/17 cells (ATCC, CRL-11268) were each transfected (Xfect, Takara #631318) with a DNA cocktail containing the 1 ...
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bioRxiv - Biochemistry 2022Quote: A HeLa-T-PFK1-mEGFP stable cell line (hereafter, HeLa-T-PFK1G) was generated using the HeLa Tet-On® 3G inducible expression system (Clontech) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... activated CD8+ T cells were transduced with MSCV-ASNS-OE or MSCV-EV viral particles on RetroNectin-coated plates (Takara Bio). 24 hours later ...
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bioRxiv - Bioengineering 2024Quote: AAV-vector plasmid and AAV-crTSG were used for AAV9 production and purification using Lenti-X 293 T cells (Takara Bio). Each set of Lenti-X 293 T cells (6 150mm-plates ...
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bioRxiv - Synthetic Biology 2023Quote: ... T cells were incubated at 3×104 cells/well in 96-well U bottom plates for 24 hours in T cell media containing 50 IU/mL rhIL-2 and varying concentrations of AP20187 (B/B homodimerizer, Takara, 635058). After 24 hours ...
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bioRxiv - Neuroscience 2024Quote: ... delta8.9 (packaging vector) and VSV-G (envelope vector) into Lenti-X 293 T cell line (632,180; Clontech, Mountain View, CA, USA) (19) ...
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bioRxiv - Cell Biology 2022Quote: ... HeLa cells stably expressing the tetracycline-controlled transactivator protein (Tet-ON, Clontech) were cultured in DMEM with 10% FBS and grown at 37°C with 5% CO2 ...
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bioRxiv - Genomics 2020Quote: ... Amplicons were ligated to T-Vector pMD19 (Takara, No.3271) with T4 ligase (NEB ...
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bioRxiv - Molecular Biology 2022Quote: ... Purified DNA was directly ligated with pMD18-T vector (Takara) for TA cloning according to the pMD18-T kit protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... and cloned into pMD19-T vector (Takara Bio, Dalian, China) before transformation into E ...
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bioRxiv - Molecular Biology 2023Quote: ... Monoclonal U-2OS cell lines stably expressing Tet-On 3G transactivator protein (Clontech), a tandem-dimeric MS2 hairpin binding protein tagged with eYFP (MS2CP-YFP) ...