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Citations for Takara Bio :
51 - 100 of 281 citations for Octreotide acetate impurity C since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... and T4 (C) were determined by digerstion with QuickCut™ EcoR V (Takara), MspJI (NEB) ...
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bioRxiv - Immunology 2021Quote: ... were coated overnight at 4°C with 20 μg/ml Retronectin (Takara T100B). Viral supernatant was spun for 90 minutes at 2,000g and 30°C onto the plated retronectin and half the supernatant volume removed carefully after spinning ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated in media containing 200 μM of A/C Heterodimerizer (Takara) or ethanol control for 4h prior to lysis ...
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bioRxiv - Pathology 2023Quote: ... Affinity purification was performed at 4°C using Talon metal affinity resins (Clontech, TaKaRa Biomedical Technology [Beijing] Co. ...
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bioRxiv - Neuroscience 2023Quote: ... DCX: doublecortin (C-18) goat polyclonal IgG (1:200, Takara Bio, Cat# 632496). NeuN ...
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bioRxiv - Cell Biology 2023Quote: ... cells were treated with the Rapalog A/C hetero-dimerizer rapalog (635057, Takara) for 24 h ...
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bioRxiv - Cell Biology 2024Quote: ... transfected cells were treated with 500 nM of A/C Heterodimerizer (Takara #635057) at the onset of live imagining ...
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bioRxiv - Molecular Biology 2024Quote: ... The PCR products were cloned into pCMV-(DYKDDDDK)-C vector (Clontech, CA, USA) using Gibson Assembly® Master Mix (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... and PMSF) followed by incubation for 20min at 37°C with 0.3U MNase (TAKARA). After the incubation ...
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bioRxiv - Genomics 2020Quote: ... and 72°C for 5 s) on the PCR Thermal Cycler Dice (Takara Bio) using PrimeSTAR MAX DNA polymerase (Takara Bio) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 72°C 1’) of target regions with Ex Taq HS DNA polymerase (Takara Bio). The PCR products were purified enzymatically with ExoSAP-IT Express PCR product cleanup (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... The cDNA fused to a C-terminal HA tag was subcloned into pQXCIH (Clontech) in between the NotI and PacI sites to obtain the pQXCIH-TMPRRS2-HA vector.
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bioRxiv - Microbiology 2021Quote: ... and hybridisation was performed overnight at 40 °C in ExpressHyb Hybridisation Solution (Clontech, USA). Membranes were then rinsed and washed at 40 °C 4 x 15 min with a wash buffer containing 1% sodium dodecyl sulphate [SDS] and 1x saline-sodium citrate (SSC) ...
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bioRxiv - Plant Biology 2021Quote: ... and c-DNA was prepared using 2 µg total RNA samples (Takara Bio, UK). The c-DNA samples were diluted ten times and an aliquot of 2 µl of each sample per reaction was used for qRT-PCR ...
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bioRxiv - Cell Biology 2021Quote: ... AXIN1 and AURKA were expressed by inserting these cDNAs into pEGFP-C (Takara/Clontech). Transient transfection of cells with plasmids was performed using Effectene transfection reagent (Qiagen ...
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bioRxiv - Cell Biology 2021Quote: ... AXIN1 and AURKA were expressed by inserting these cDNAs into pEGFP-C (Takara/Clontech). Transient transfection of cells with plasmids was performed using Effectene transfection reagent (Qiagen ...
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bioRxiv - Synthetic Biology 2021Quote: ... Rapalog AP21967 (also known as A/C heterodimerizer, purchased from Takara Biosciences; catalog# 635056) is a synthetic rapamycin analog that can bind with FRB harboring the T2098L mutation ...
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bioRxiv - Biophysics 2022Quote: ... and human CaMKIIK42RD135N with a C-terminal AviTag were cloned into pEGFP-N1 (Clontech) vector ...
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bioRxiv - Neuroscience 2024Quote: ... and at 72°C for 30 sec in TaKaRa Thermal Cyclar Dice Touch (Takara).
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bioRxiv - Pathology 2023Quote: ... Affinity purification was performed at 4°C using Talon metal affinity resins (Clontech, TaKaRa Biomedical Technology [Beijing] Co. ...
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bioRxiv - Bioengineering 2023Quote: ... 37°C for 2 hours in a plate coated with Retronectin (Takara Bio, T100B) with an MOI of ∼5.0 for each lentivirus (dCas9 lentivirus at MOI ∼5.0 and gRNA-MCP-fusion effector lentivirus) ...
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bioRxiv - Biochemistry 2023Quote: ... CBS proteins with a permanent C-terminal His-tag were purified using TALON (Clontech) resin followed by anion exchange using a Hitrap Q column (Cytiva) ...
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bioRxiv - Cell Biology 2023Quote: ... TagRFP-CENP-E 2111-C were cloned into the pLVX-IRES-Puro vector (Clontech) by PCR-based Gibson assembly method.
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bioRxiv - Synthetic Biology 2023Quote: ... Rapalog AP21967 (also known as A/C heterodimerizer, purchased from Takara Biosciences; catalog# 635056) was administered at the time of transfection ...
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bioRxiv - Neuroscience 2023Quote: ... the mouse monoclonal anti-GFP (Jl-8, Clontech; 1:500 overnight at 4°C) primary antibody was used ...
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bioRxiv - Physiology 2024Quote: ... The TBX15 sequence was subcloned into a C-terminal tagged HA vector (636590, TaKaRa)_by PCR amplification (Q5 polymerase ...
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bioRxiv - Cell Biology 2024Quote: ... cells were treated with 500 nM Rapalog A/C hetero-dimerizer rapalog (635057, Takara) for 24 h ...
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bioRxiv - Immunology 2022Quote: ... and fused to the C-terminus of mNeonGreen-F-Tractin using In-Fusion cloning (Takara). As a control ...
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bioRxiv - Genomics 2020Quote: ... 5 × 104 cells were stored at −80 °C in STEM CELLBANKER® (Takara Bio Inc.) until use ...
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bioRxiv - Cell Biology 2021Quote: ... C-terminally triple FLAG tagged MBP was inserted into pKK plasmids using InFusion cloning (Takara). To introduce C-terminally triple FLAG tagged UPF1 ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.1%BSA and 10% FBS.Cells were incubated with anti-c-Myc antibody (Clontech Laboratories, USA) at a 1:10 dilution for 16 hr at 4°C ...
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bioRxiv - Immunology 2021Quote: ... containing chilled (4°C) RNA lysis buffer (SMART-Seq HT lysis buffer, Takara Clontech, #634439). This contained the oligo-dT primer ...
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bioRxiv - Immunology 2021Quote: ... containing chilled (4°C) RNA lysis buffer (SMART-Seq HT lysis buffer, Takara Clontech, #634439). This contained the oligo-dT primer ...
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bioRxiv - Systems Biology 2022Quote: ... Cells were then treated with 200nM rapalog (A/C heterodimerizer, previously AP21967) (Takara Bio., 635055) and immediately subjected to live cell microscopy with images being taken every 30 minutes for 12 hours ...
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bioRxiv - Cell Biology 2023Quote: ... cells were pre-treated 1 hour prior to the assay with A/C Heterodimerizer (Takara) diluted at 1:200 in complete media concurrent with JF-Halo or -SNAP dye labeling ...
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bioRxiv - Cell Biology 2023Quote: ... and inserted into the pScarlessHD-C-3xVHH05-6XMS2-DsRed vector via InFusion cloning (Takara Bio). The DNA plasmid of pScarlessHD-5’ msps homology arm-C-3xVHH05-6XMS2-DsRed-3’ msps homology arm was co-injected with pCFD5-gRNA#1 and pCFD5-gRNA#2 by BestGene ...
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bioRxiv - Genomics 2024Quote: ... The protease and cleaved C-terminal tags were removed using Ni-NTA resin (Takara, 635662), and the proteins were further purified by size-exclusion chromatography on a HiLoad 26/600 Superdex 200 pg (Cytiva) ...
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bioRxiv - Cell Biology 2024Quote: ... cells were treated with either rapalog A/C heterodimerizer (final concentration 5μM; Takara, Cat# AP21967) or 200 μL ethanol (control) ...
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bioRxiv - Cell Biology 2024Quote: The following chemicals were used in this study: Rapalog A/C hetero-dimerizer (635057, Takara), Bafilomycin A1 (sc-201550 ...
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bioRxiv - Molecular Biology 2020Quote: ... The membrane was dried and pre-hybridized at 37 °C for 30 min in ExpressHyb (Clontech). Hybridization was performed in ExpressHyb containing RI-labeled probe at 37 °C overnight with rotation ...
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bioRxiv - Cell Biology 2020Quote: The GST-Bbs5 was expressed for 3 h at 27 °C in Escherichia coliBL21(DE3) (Takara) transformed with pGEX-4T-2-Bbs5-WT in the presence of 0.1 mM isopropyl-β-D-thiogalactopyranoside (Takara) ...
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bioRxiv - Cell Biology 2022Quote: ... Lentiviral media was then kept at 4°C before 100x concentration using LentiX concentrator (Takara #631232) and resuspension of virus in organoid seeding media ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Membranes were baked for 2 h at 80°C and prehybridized in ExpressHyb (Clontech, Mountain View) at 65°C for 1h ...
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bioRxiv - Neuroscience 2021Quote: ... 72°C for 15 s performed using a Takara Thermal Dice Real-time system III (Takara) or a QuantStudio 6 Flex Real-time PCR system (Applied Biosystems ...
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bioRxiv - Neuroscience 2021Quote: ... Ank only) rat Shank3 with a C-terminal mRFP-tag were generated in pmRFP-N3 (Clontech). A construct coding for N-terminally GFP-tagged full-length rat Shank3 in the pHAGE vector was obtained from Alex Shcheglovitov (Univ ...
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bioRxiv - Neuroscience 2022Quote: ... we carry out all steps at 4°C and in the presence of RNase inhibitor (Takara). We use the isolated nuclei for the droplet-based 10x scRNA-seq assay ...
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bioRxiv - Cell Biology 2020Quote: ... Constructs for C-terminal GFP-tagged human RHBDL4 were generated by subcloning into pEGFP-N1 (Clontech). For generating point mutants ...
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bioRxiv - Neuroscience 2023Quote: ... pAcGFP-His-MAP2C without AcGFP was amplified and Dendra2 was amplified from pDendra2-C vector (Takara). Dendra2 was inserted into where AcGFP was by In-Fusion cloning kit ...
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bioRxiv - Cell Biology 2023Quote: Procollagen Type 1 C-peptide (PIP) was measured according to the manufacturer’s instructions (Takara, Shiga, Japan). For evaluation of PIP ...
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bioRxiv - Biophysics 2024Quote: ... and the supernatant was incubated overnight at 4 °C with Co2+-charged affinity resin (Talon, Clontech) and 30 mM imidazole.