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Citations for Takara Bio :
51 - 100 of 2140 citations for FH1 FH2 domain containing protein 1 FHOD1 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... was co-transformed with GAL4 DNA-BD-fused IPMK and a human brain cDNA activation domain (AD) library (Clontech). Two different reporter genes (HIS3 and ADE2 ...
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bioRxiv - Molecular Biology 2021Quote: ... Coding regions of the heavy-chain variable domains were amplified using LA Taq polymerase (TAKARA Bio Inc., Shiga, Japan) with two PAGE-purified primers (CALL001 ...
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bioRxiv - Developmental Biology 2021Quote: ... and as a prey to the activation domain of the GAL4 transcription factor (GAL4-AD) in plasmid pACT2 (Clontech). To monitor the induction of a HIS3 reporter gene by complexes of bait and prey fusion proteins ...
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bioRxiv - Immunology 2021Quote: ... His-tagged NTD domain constructs were purified from clarified supernatants using 2 ml of cobalt resin (Takara Bio TALON), washing with 50 column volumes of 20 mM HEPES-HCl pH 8.0 and 150 mM NaCl and eluted with 600 mM imidazole ...
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bioRxiv - Microbiology 2020Quote: ... ramulus DSM 15684 were cloned without signal peptide or transmembrane domain from genomic DNA using In-Fusion cloning (Takara) and the primers in Table S8 into the EcoRI and Ncol restriction sites of the corresponding plasmids ...
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bioRxiv - Plant Biology 2022Quote: CRISIS2 or Nb14-3-3 was fused with the Gal4 DNA binding domain in pGBKT7 (Clontech, PT3248-5, USA) and inserted into the Saccharomyces cerevisiae Y2HGold strain (Clontech ...
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bioRxiv - Plant Biology 2024Quote: ... the full-length ORF of PpGL2 fused with the GAL4 DNA binding domain in the PGBKT7 vector (Clontech, Japan) was transformed into the competent yeast strain Y2HGold ...
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bioRxiv - Plant Biology 2023Quote: ... fused with GAL4 activation domain was amplified and subcloned into the prey vector pGADT7 (Clontech Laboratories, Inc., Kusatsu, Japan). Competent yeast cells were prepared according to the Clontech Yeast protocols handbook using the Y1H Gold yeast strain ...
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bioRxiv - Neuroscience 2024Quote: The coding region of s1pr1 was fused to the GAL4 binding domain of the “bait” pBT3-STE vector (Clontech) cloning at Sfi IA (5′-GGCCATTACGGCC-3′ ...
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bioRxiv - Cell Biology 2024Quote: ... AcrIIA4 was cloned into pENTR dTOPO and SMASh or dTAG domains were inserted via Gibson assembly (Clontech, cat. # 638909) to be in frame with AcrIIA4 ...
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bioRxiv - Neuroscience 2023Quote: ... sagittal sections (50 µm) of the cerebellum were cut and incubated with antibodies against the reporter protein mCherry (Clontech 632543, RRID: AB_2307319, 1:500) and against the P-cell marker calbindin (Swant CB38 ...
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bioRxiv - Immunology 2021Quote: ... containing 2 μl lysis buffer per well (1:20 RNase inhibitor (Clontech) in 0.2% (v/v ...
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bioRxiv - Biochemistry 2021Quote: ... monoclonal antibody for green fluorescent protein (JL-8; Takara Bio, Kusatsu, Japan; recognizes VC), rabbit anti-GFP (D5.1 Cell Signaling Technology 2956S ...
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bioRxiv - Plant Biology 2022Quote: ... CITRINE-fusion proteins were detected with anti-GFP antibody (JL-8, Takara Bio Clontech, dilution ...
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bioRxiv - Plant Biology 2021Quote: ... GFP-tagged proteins were detected using the anti-GFP antibody (JL-8, Clontech Takara) diluted 1:2000 (v/v ...
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bioRxiv - Plant Biology 2021Quote: ... GFP-tagged proteins were detected using the anti-GFP antibody (JL-8, Clontech Takara) diluted 1:2000 (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... GFP fusion proteins were revealed with mouse monoclonal antibody anti-GFP (JL-8, Clontech) used at 1/2000 dilution ...
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bioRxiv - Neuroscience 2022Quote: ... slices were incubated in a blocking buffer containing the primary antibodies rabbit anti-mCherry (1:1500; cat. No. 632496, Takara Bio USA, Inc., Mountain View, CA, USA) and chicken anti-GFP (1:1500 ...
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bioRxiv - Molecular Biology 2024Quote: ... 4.9 μg DNA was transfected per dish containing a 1:1 molar ratio of pmCherry-N1 (Clontech) and of the pUC18-GFP or linear GFP substrate ...
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bioRxiv - Molecular Biology 2024Quote: pEGFPmneoI: is a pCEP4 based vector containing the enhanced green fluorescent protein (EGFP) ORF sequence from plasmid pEGFP-C3 (Clontech) followed by the mneoI retrotransposition indicator cassette ...
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bioRxiv - Molecular Biology 2024Quote: EGFPmneoI: is a pCEP4 based vector containing the enhanced green fluorescent protein (EGFP) ORF sequence from plasmid pEGFP-C3 (Clontech) followed by the mneoI retrotransposition indicator cassette ...
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Targeted attenuation of elevated histone marks at SNCA alleviates α-synuclein in Parkinson’s diseasebioRxiv - Neuroscience 2020Quote: ... Anti-Cas9 antibody (Takara, 632607; 1:1000) and anti-GFP antibody (Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... GFP monoclonal antibody (Takara, # 632375; 1:2,000), Bip2 antibody (Agrisera ...
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bioRxiv - Molecular Biology 2022Quote: ... anti-GFP antibody (Clontech, rabbit, 1:2000), anti-Ago1 antibody (Abcam ...
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bioRxiv - Microbiology 2023Quote: ... monoclonal antibody (cat # 632380, 1: 2,000, Clontech) and horseradish peroxidase (HRP)-labeled anti-mouse IgG (H + L ...
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bioRxiv - Genomics 2023Quote: ... Antibody list: FOXG1 (rabbit, 1:200, Takara) and PAX6 (mouse ...
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bioRxiv - Biophysics 2020Quote: Orai1-CAD-CAD-GFP constructs were designed by cloning Orai1 and STIM1-CAD domains (aa344-449) into peYFP-N1 (Clontech). Linker regions between Orai1 and CAD (Orai1-linker-1-CAD-linker-2-CAD-YFP ...
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bioRxiv - Cell Biology 2022Quote: ... the PCDH15-CD2 isoform cytoplasmic domain was cloned using the HindIII and BamHI restriction sites of the pmCherry-C2 vector (Clontech), resulting in soluble protein tagged with cherry at its N-terminus.
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bioRxiv - Molecular Biology 2021Quote: ... Gal4-VP16-human SREBP-1c plasmid was prepared by insertion of a VP16-transactivation domain fused to a human SREBP-1c fragment from the 431st amino acid to the C-terminus (amino acids 431-1123) downstream of the Gal4-DNA binding domain sequence in pM vector (Clontech). The Gal4-RE-Luc plasmid and luciferase plasmid including sterol response element (SRE-Luc ...
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bioRxiv - Microbiology 2020Quote: ... cDNA library derived from soybean hypocotyl and roots RNA were constructed to pGADT7 plasmid fused with GAL4 activation domain (AD) by Clontech company ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNAs were amplified using the corresponding primers (Supplementary Table S3) and fused with the DNA-binding or activation domain of GAL4 in the corresponding pGBT9 and pGAD424 vectors (Clontech). Details of assembling the constructs for expressing proteins in transgenic flies are available upon request.
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bioRxiv - Plant Biology 2022Quote: ... GID1b and GID1c cDNAs inserted into pDONR221 were fused to the activation domain GAL4 (AD) after recombination into pGADT7 (Clontech).
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bioRxiv - Microbiology 2021Quote: ... Forward and reverse primers carrying the above restriction sites were designed to amplify a 3kb fragment from Diaph2 MGC human cDNA clone (Dharmacon) and the amplicon encompassing the entire cDNA sequence minus the DAD domain was cloned into pLVX-mCherry-N1 (Clontech) to generate lentiviral expression vector pLVXdeltaDADmcherry ...
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bioRxiv - Plant Biology 2020Quote: The transformation of each plasmid for the respective domain configurations was performed as described by Gietz and Schiestl (2007) using Saccharomyces cerevisiae strain HF7C (Clontech).
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bioRxiv - Plant Biology 2021Quote: ... fragments containing a stop codon were recombined into Gateway-compatible versions of the GAL4 DNA BD vector pGBT-9 and the activation domain vector pGAD424 (Clontech) using L/R-clonase (ThermoFisher) ...
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bioRxiv - Microbiology 2021Quote: ... yeast containing the Gal4 activation domain-based prey and the Gal4 DNA-binding domain-based bait vectors were grown overnight in SD-Leu-Trp broth (Clontech). The OD600 value was recorded ...
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bioRxiv - Cell Biology 2020Quote: ... Point mutants of the microtubule-binding domain were created using site-directed mutagenesis using PrimeSTAR Max DNA polymerase (Takara Bio).
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bioRxiv - Plant Biology 2022Quote: ... The CWLP coding region without signal peptide (SP) was cloned in frame downstream of the GAL4 DNA-binding domain in the bait vector pGBKT7 (Clontech) and introduced into the yeast strain AH109 (Clontech) ...
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bioRxiv - Microbiology 2023Quote: ... Fragments encoding the CD45 extracellular domain (ECD) and transmembrane region were amplified by PCR and cloned into pEGFP-N1 (Clontech) using the EcoRI and BamHI restriction sites ...
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bioRxiv - Plant Biology 2024Quote: ... the full-length ORF of PpGL2 was fused with the GAL4 transcriptional activation domain in the pGADT7 vector (Clontech, Japan), and then co-transformed with the pHIS2 cis reporter vector (containing triple tandem CAAATTG repeat sequence ...
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bioRxiv - Molecular Biology 2024Quote: The RBD-DsRed plasmid was created by PCR cloning the HB domain of RNAse H1 into the pDsRed-Express-C1 vector (Clontech), following the previously described method [32].
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bioRxiv - Immunology 2022Quote: ... Mate & Plate™ cDNA Library in a pGADT7 vector with the GAL4 DNA-activation domain (AD) and pre-transformed into yeast strain Y187 was acquired from Takara Bio ...
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bioRxiv - Evolutionary Biology 2023Quote: ... A total of 500 ng of sheared DNAs was input for methyl-CpG binding domain (MBD) enrichment using the EpiXplore Methylated DNA Enrichment Kit (Clontech) according to the manufacturer’s instruction ...
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bioRxiv - Plant Biology 2023Quote: ... The LexA fusion constructs (bait) and the activation domain fusion constructs (prey) were co-transformed into yeast strain EGY48[p8op-lacZ] (CLONTECH). Clones containing both constructs were selected on medium lacking His ...
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bioRxiv - Plant Biology 2023Quote: ... pGADT7 plasmids encoding the effector domain of different ZiF were co-transformed into chemically competent Y2HGold cells (Takara Bio, USA) with a pGBKT7 plasmid encoding OsExo70F3 in using a Frozen-EZ Yeast Transformation Kit (Zymo research).
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bioRxiv - Biochemistry 2024Quote: ... Plant targets identified in the yeast-two hybrid screen were cloned as translational fusions with the activation domain in pB42AD (Clontech) for verification ...
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bioRxiv - Genetics 2024Quote: ... and PAR2 domain was amplified from HEK 293T cDNA and all three was cloned in frame together using In-Fusion cloning(Takara) in the background of TOPO pCR2.1 vector(Thermo Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... the primary antibody was anti-dsRed antibody (dilution 1:1000, Clontech, Cat #632496), and the second antibody was goat anti-rabbit conjugated with Cy3 (Jackson ImmunoResearch ...
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bioRxiv - Cell Biology 2023Quote: ... E-cadherin antibody (HECD-1, 1:1000 IF, 1:1000 WB) was from Takara Bio ...
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bioRxiv - Cell Biology 2020Quote: The cDNA coding sequences of the first and second cytoplasmic loop domain of human TMEM39A were cloned into the pGBKT7 vector and screened against a normalized universal human cDNA library (Clontech, 630481), following instruction of the Matchmaker® Gold Yeast Two-Hybrid System (Clontech ...