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Citations for Takara Bio :
51 - 68 of 68 citations for ALLYL 11 12 EPOXY STEARATE TECH since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Retroviral supernatants were then spun for 1 h at 3100g at 4 °C in 12-well-plates coated with 32 mg/ml RetroNectin (Takara). Meanwhile ...
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bioRxiv - Cancer Biology 2024Quote: ... non-tissue culture treated 12-well plates were coated overnight at 4°C with 25 μg/ml retronectin in PBS (Takara). Plates were washed with PBS and blocked with PBS supplemented with 2% BSA for 15 min ...
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bioRxiv - Immunology 2021Quote: ... Aliquots of 1 Mio cells in 1 ml medium were grown overnight in 12- or 24-well plates (either TC-treated or coated with 5 µg/cm2 retronectin [Takara Bio]) and then transduced with VSV-pseudotyped lentivirus encoding for either the 1G4 or the A6 TCR ...
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bioRxiv - Immunology 2020Quote: ... Aliquots of 1 Mio cells in 1 ml medium were grown overnight in 12-or 24-well plates (either TC-treated or coated with 5 μg/cm2 retronectin [Takara Bio]) and then transduced with VSV-pseudotyped lentivirus encoding for either the 1G4 or the A6 TCR ...
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bioRxiv - Bioengineering 2023Quote: ... On Day 2 virus coated culture plates were prepared on non-TC-treated 12-well plates that had been pre-coated with RetroNectin (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... In short: 3.5 cm non-coated cell culture dishes (Falcon, cat 351008) were coated using 12 µg Retronectin (Takara, cat T100B). Coated dishes then were pre-incubated with 1 ml viral supernatant for 4-5 hours at 37oC ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were then cleared by centrifugation at 12,000xg for 12 minutes and added to 1ml of pre-equilibrated TALON metal affinity resin (Takara Bio, 635502). The slurry was rotated at 4°C for 1 hour ...
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bioRxiv - Plant Biology 2024Quote: ... Samples were then run on a 4-12% Bis-Tris gel and transferred to a membrane for western blotting using α-GFP (Clontech) and α-HA (Cell Signaling Technology ...
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bioRxiv - Synthetic Biology 2023Quote: ... On Day 2 virus coated culture plates were prepared on non TC-treated 12-well plates that had been pre-coated with RetroNectin (Takara Bio) according to the manufacturer’s instructions ...
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The spindle protein CKAP2 regulates microtubule dynamics and ensures faithful chromosome segregationbioRxiv - Cell Biology 2023Quote: ... pEF1α-TET3G-stable cell lines were then transfected with a pTRE3G-CKAP2:mGL plasmid containing the PTRE3G promoter activatable upon doxycycline exposure and cultured in regular DMEM/F-12 media supplemented with Tet-Approved FBS free of tetracycline contaminants (Takara Bio; 631105). Prior to experiments ...
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bioRxiv - Neuroscience 2024Quote: Quantitative PCR was performed in 12 μl of final volume on 96-well plates using the Premix Ex Taq (Takara Biotechnology, #RR037A). Reactions included Segment 1 ...
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bioRxiv - Biophysics 2024Quote: ... both flasks were washed with 15 mL PBS+/+ and one of them was incubated with 12 mL NDiff227 media (N2B27) (Takara Bio, #Y40002) containing 1.6 µM SiR-DNA (Spirochrome ...
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bioRxiv - Biophysics 2020Quote: ... was cultured under the following conditions: cells were maintained in DMEM/F-12 50/50 medium containing 10% fetal bovine serum (tetracycline-free, Takara, Mountain View, CA), penicillin (100 units/ml) ...
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bioRxiv - Neuroscience 2020Quote: ... The reaction was performed in a final volume of 12 μL using the Premix Ex Taq Probe based qPCR assay (Takara Biotechnology, ref. RR390A). All reactions were run in duplicate ...
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bioRxiv - Bioengineering 2023Quote: ... and 12 ng of total RNA was reverse-transcribed to generate cDNA using PrimeScript RT Master Mix (Perfect Real Time; TaKaRa Bio, Shiga, Japan) after cell sorting ...
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bioRxiv - Molecular Biology 2023Quote: ... An RNA amount equivalent to 12 ng was used to prepare the sequencing library using SMARTer® smRNA-Seq Kit for Illumina® (Takara 635031) according to the kit manual till cDNA synthesis ...
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bioRxiv - Neuroscience 2023Quote: ... The qPCR reaction was performed on 96-well plates in a final volume of 12 μL using the Premix Ex Taq Probe based qPCR assay (Takara Biotechnology, ref. RR390A). All reactions were run in duplicate on a StepOnePlus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Cell Biology 2020Quote: ... Protein samples were run on a 4-12% Bis-Tris gel (Novex) and Western blots were performed with the following antibodies: α-GFP (Clontech Living Colors 632381 (JL-8); RRID:AB_2313808 ...