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Citations for Takara Bio :
901 - 950 of 2005 citations for Mouse Anti Human Immunodeficiency Virus HIV 1 gp41 1911 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... Rab 5 and Rab 7 cDNA (a gift of M. Sandor) and human CD81 cDNA (Open Biosystems) were cloned into pmCherry-N1 vector (Clontech). Raji/DC-SIGN cells were electroporated with 1 µg of indicated plasmid using the Eppendorf Multiporator in iso-osmolar electroporation buffer using a 90 µs ...
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bioRxiv - Cell Biology 2019Quote: ... The coding sequences of these small GTPase proteins were amplified with polymerase chain reaction (PCR) using KOD-Plus-Neo DNA polymerase (Toyobo) and Human Universal QUICK-Clone cDNA II (Clontech) as template cDNA ...
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bioRxiv - Molecular Biology 2019Quote: ... Human ZNRF2 was obtained from the Orfeome clone collection (ID 47920) and transferred by Gateway cloning into pGBT9-GW (Clontech). GAD-E2 and GBD-E3 constructs were combined by transformation into PJ69-4α and PJ69-4a ...
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bioRxiv - Cell Biology 2020Quote: ... of human ACLY was PCR amplified from a HeLa cDNA pool and inserted into the EcoRI-digested pLVX-puro vector (Takara) using the In-Fusion cloning system (Takara) ...
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bioRxiv - Microbiology 2021Quote: For human ectopic expression plasmids: Full length cDNA for each gene was amplified from a HeLa cDNA library (Takara Bio) and inserted into a pCDNA expression plasmid modified with either a C-terminal HA or N-terminal V5 tag under the human cytomegalovirus (CMV ...
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bioRxiv - Neuroscience 2021Quote: Plasmids encoding GST-BVR (GST-BVRα) and GST-BVRβ were generated by cloning cDNA of human BLVRA and BLVRB into pCMV-GST vector (Clontech/TaKaRa). The construct encoding myc-FAK was generated as previously described (95) ...
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bioRxiv - Cell Biology 2021Quote: ... pDAA-002 encodes a C-terminal FLAG-tagged version of human PKR hosted in the retroviral expression vector pLPCX (Clontech) and was generated by cloning a PCR product (PCRP ...
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bioRxiv - Neuroscience 2023Quote: The high-quality human total brain RNA that was used for 3’ RACE was purchased from Clontech (Mountain View, CA). SCA12 KI-10 and KI-80 mouse models were generated using the CRISPR/Cas9 approach by replacing the mouse PPP2R2B exon 2 with the human PPP2R2B exon 7 containing either 10 or 80 CAG triplets (Li and Margolis ...
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bioRxiv - Microbiology 2023Quote: A yeast screen for human cellular interacting proteins of pUL136 was performed using the Matchmaker Gold Yeast Two-Hybrid System (Clontech) and the Mate and Plate Universal Human Library (Clontech) ...
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bioRxiv - Biochemistry 2023Quote: ... was generated by amplifying the coding sequence of human EL from cDNA (LIPG; MGC MHS6278-202806078) and inserting it into the vector pCDNA6 using InFusion cloning (Clontech). An EL containing a T111I mutation (pKS17 ...
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bioRxiv - Cell Biology 2023Quote: ... pLVX-DsRed-Monomer-N1–hVMP1 was obtained by inserting the human VMP1 sequence (NCBI reference sequence: NM_030938.5) into pLVX-DsRed-Monomer-N1 (CLONTECH cat. 632152). pLenti–VMP1–GFP was obtained by cloning the VMP1 sequence (NCBI reference sequence ...
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bioRxiv - Biochemistry 2023Quote: ... FL-human KANK1 (generous donation from the Bershadsky lab) cDNA was tagged in the C-terminal site with pmCherry (Clontech) by restriction digestion ...
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bioRxiv - Cell Biology 2023Quote: ... The DNA fragments encoding human zyxin were amplified from the cDNA of U2OS cells and then inserted into the pmCherry-N1 vector (Clontech) for the expression of zyxin-RFP.
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bioRxiv - Cell Biology 2023Quote: ... The PISD-FLAG plasmid was generated by amplifying human PISD from cDNA and cloning it into the BglII and SalI sites of the pmCherry-N1 vector (Clontech). The C-terminal mCherry tag was subsequently replaced with 3xFLAG tag using the AgeI and NotI restriction sites ...
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bioRxiv - Developmental Biology 2023Quote: ... Quantitative RT-PCR for pluripotency and trilineage spontaneous differentiation was performed according to the instruction manual of the human ES cell Primer Array (Takara Clontech).
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bioRxiv - Cell Biology 2023Quote: ... mEmerald/ mApple -VTA1: full-length human VTA1 was amplified by PCR and cloned to mEmerald or mApple -C1 vectors (Clontech). mCh-CHMP4C ...
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bioRxiv - Cell Biology 2024Quote: A PCR product containing the Human ORF for DDX3X was obtained directly from RNA using the Primescript High Fidelity RT-PCR kit from Takara. The PCR primers introduced BamHI and NotI sites at the 5’ and 3’ ends respectively ...
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bioRxiv - Synthetic Biology 2024Quote: WT Human Embryonic Kidney (HEK) 293 cells (ATCC, catalog no. CRL-1573) and HEK293T-LentiX (Takara Biosciences, catalog no. 632180) were cultured in a humidity-controlled incubator under standard culture conditions (37 °C with 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... cDNA libraries were generated using SMARTer Human TCR a/b Profiling Kit v2 (Takara Bio USA, San Jose, California, USA). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: ... The N-terminal His tag construct of human MPST was co-transformed with GroES-EL chaperon plasmid from Takara (#3340), overexpressed in E ...
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bioRxiv - Systems Biology 2024Quote: SW1353 cells were purchased from ATCC Full length miRNA target 3’UTRs were amplified from human genomic DNA using PCR primers (Supplementary Table 4) to enable Clontech In-Fusion HD cloning (Takara Bio Europe ...
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bioRxiv - Cell Biology 2019Quote: ... for 30 min at 37°C in a moisture chamber followed by an incubation with anti-GFP (1:100 in PBSA) antibody at 4°C for 72 h (Takara 632381/JL-8)) ...
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bioRxiv - Physiology 2019Quote: ... Membranes were incubated at 4°C in primary antibodies diluted 1:1000 in 5% bovine serum albumin: anti-GFP (ClonTech Living Colours #ab632375), anti-HSP60 (Department of Biology ...
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bioRxiv - Neuroscience 2024Quote: ... The primary antibody used was rabbit-anti-dsRed (1:500, Living Colors DsRed pAb, RRID: AB_10013483; Takara Bio USA, Mountain View, CA, USA) to enhance mCherry-labeled rVRG axon visualization ...
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bioRxiv - Genetics 2019Quote: ... Antibodies used were GAL4 AD Mouse Monoclonal Antibody (Takara Bio USA, Inc. #630402), GAL4 DNA-BD Mouse Monoclonal Antibody (Takara Bio USA ...
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bioRxiv - Immunology 2019Quote: ... The cDNA was generated and amplified by SMARTer Mouse TCRαβ Profiling Kit (Clontech). Libraries were sequenced by illumina sequencer MiSeq (2×250 cycles) ...
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bioRxiv - Immunology 2021Quote: ... Blots were stained with the mouse α-GFP or α-mCherry (Clontech, EU) antibodies.
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bioRxiv - Neuroscience 2020Quote: Mouse Nwd1 cDNAs were subcloned into the pEGFP-C2 vector (Clontech Takara Bio) to express the Nwd1 protein fused with EGFP ...
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bioRxiv - Cell Biology 2020Quote: The mouse Tacc3 sequence was amplified by PrimeSTAR® Max DNA polymerase (TAKARA) using forward 5’-CTCCCCAGGGGGATCATGAGTCTGCATGTCTTAAAT-3’ and reverse 5’-GAGGTTGATTGTCGATCAGATCTTCTCCATCTTAG-3’ primers ...
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bioRxiv - Cell Biology 2022Quote: Mouse Itgb3 was cloned into the pLVX-EF1α-IRES-Puro vector (Takara Bio) per a previously described cloning strategy (Dave et al. ...
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bioRxiv - Immunology 2022Quote: ... or splenocytes from a naïve C57BL/6 mouse using NucleoSpin RNA Plus (Takara). RNA was reverse transcribed with SuperScript III RT and random primers (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... A mouse 7-day embryo cDNA Library (CATALOG No. 630478; Clontech Laboratories, Inc.) was used to identify host interaction proteins of Mtb PknG through yeast two-hybrid assay ...
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bioRxiv - Cell Biology 2022Quote: Every protein expressed in mouse rods was also cloned into pEGFP-N1 (Clontech) for expression in AD293 cells using the AgeI and NotI cloning sites within the vector to replace EGFP with the tagged proteins ...
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bioRxiv - Neuroscience 2020Quote: Mouse Nwd1 cDNAs were subcloned into the pEGFP-C2 vector (Clontech Takara Bio) to express the Nwd1 protein fused with EGFP ...
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bioRxiv - Microbiology 2023Quote: ... and mouse GAPDH transcripts as described previously 47 using SYBR green reagent (Takara) with primer pairs 1195/1196 ...
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bioRxiv - Molecular Biology 2021Quote: ... anti-GFP (Clontech, #632381), anti-alpha Tubulin (abcam ...
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bioRxiv - Cell Biology 2021Quote: ... monoclonal anti-GFP (Takara) and monoclonal anti-tubulin (T5168 ...
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bioRxiv - Microbiology 2020Quote: ... anti-Flag (Clontech, USA) and anti-GFP (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... anti-GFP (Clontech, JL8), anti-TUBULIN (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... anti-GFP (632381, Clontech), hFAB rhodamine anti-tubulin (12004166 ...
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bioRxiv - Developmental Biology 2022Quote: ... anti-EGFP (Clontech, 632569) and anti-β-actin (Santa Cruz ...
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bioRxiv - Molecular Biology 2022Quote: ... anti-His (Clontech 631212), anti-H3K36me2 (Upstate 07-369) ...
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bioRxiv - Cancer Biology 2021Quote: ... anti-GFP (Clontech, 632460), LAMP1 (Abcam ...
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bioRxiv - Developmental Biology 2019Quote: ... anti-L7 (Takara, M202), anti-PAX2 (Abcam ...
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bioRxiv - Plant Biology 2019Quote: ... anti-GFP (Clontech #632381), anti-MYC antibodies (Millipore #05-724 ...
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bioRxiv - Biochemistry 2021Quote: ... anti-EGFP (#632381, Takara), anti-mCherry (#PA534974 ...
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bioRxiv - Developmental Biology 2020Quote: ... rabbit anti-dsRed (Clontech) 1:200 ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit anti-DsRed (TaKaRa Bio ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit anti-DsRed (Clontech), guinea-pig anti VGLUT1 (Synaptic Systems ...
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bioRxiv - Microbiology 2023Quote: ... anti-GAPDH antibody (Clontech) at a 1/2500 dilution ...