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Citations for Takara Bio :
901 - 950 of 5611 citations for Human Guanylate Binding Protein 6 GBP6 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... 10 µg of Spike expressor and 2.5 µg of a green fluorescent protein (GFP) expressor (pIRES2-eGFP; Clontech) was transfected into 2 × 106 HEK 293T cells ...
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bioRxiv - Plant Biology 2022Quote: ... bait and linker proteins were cloned into the appropriate position of the pBridge vector (Clontech, Mountain View, California), which encodes a GAL4 DNA binding domain and a linker protein ...
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bioRxiv - Molecular Biology 2023Quote: ... purified MBP-mEGFP/mCherry-Seb1 and MBP-mEGFP/mCherry-Rhn1 proteins were incubated with HRV 3C protease (Takara; 1 U/1 g of target recombinant proteins ...
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bioRxiv - Plant Biology 2024Quote: ... The PLT2-GFP fusion protein abundance was detected by using anti-GFP (cat#632381, 1:3,000 dilution; Clontech) and anti-ACTIN (cat#MA1-744 ...
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bioRxiv - Microbiology 2024Quote: ... and the protein was purified using cobalt affinity resin (1mL/L culture, Takara Bio USA, San Jose, CA). The protein was washed with 100 mM NaCl ...
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bioRxiv - Cell Biology 2023Quote: ... Cre recombinase proteins were delivered into TP53-missense mutation knock-in cells using Cre Recombinase Gesicles (Takara Bio).
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bioRxiv - Microbiology 2023Quote: ... The His-tagged CRONE protein was purified by cobalt affinity chromatography using TALON® metal affinity resin (Takara) under native conditions in phosphate buffered saline (PBS) ...
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bioRxiv - Biochemistry 2024Quote: ... Recombinant protein was then purified by immobilized metal ion affinity chromatography using a cobalt-based matrix (Talon, Clontech) and ...
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bioRxiv - Cell Biology 2019Quote: Total RNA was isolated (HiPure Plant RNA MIni Kit, Magen) and reverse transcribed (The PrimeScript® RT reagent Kit, Takara). Two pairs of primers (5′-GGCAAGAATCATCACGACCAG-3′ and 5′-GTATGCCATGAGGTCGTCCAC-3′ ...
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bioRxiv - Genetics 2021Quote: Total RNA was extracted from wing discs of individual silkworms at the wandering stage using the MicroElute Total RNA Kit (OMEGA) and reverse transcription was performed using the PrimeScript RT reagent Kit (Takara). qRT-PCR experiments were performed using Hieff SYBR Green Master Mix (YEASEN) ...
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bioRxiv - Microbiology 2021Quote: ... Remaining DNA was removed using the TURBO DNA-free kit (Thermo-Scientific) and cDNA generated using the PrimeScript cDNA synthesis kit (Takara). qRT-PCR reactions were set up according to the manufacturer’s instructions (Alkali Scientific) ...
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bioRxiv - Developmental Biology 2020Quote: ... Libraries were prepared using the SMART-Seq v3 Ultra Low RNA Input Kit for Sequencing and the Low Library Prep Kit v1 from Clontech. For the an3 RNAseq experiment ...
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bioRxiv - Molecular Biology 2020Quote: ... the cDNAs were produced using BioRad Reverse Transcription Kit (iScriptTM Reverse Transcription Supermix) or the PrimeScript RT reagent kit (RR037A, TaKaRa). cDNAs from HEK and THP89 cells were quantified by quantitative PCR using Bio Rad’s SsoAdvanced Universal SYBR Green Supermix ...
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bioRxiv - Immunology 2021Quote: ... Following bulk BCR and TCR are prepared using SMARTer Mouse BCR IgG H/K/L Profiling Kit and SMARTer Mouse TCR a/b profiling kit separately (Takara). Based on the extracted mRNA amount of each sample ...
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bioRxiv - Neuroscience 2022Quote: ... RNA with RIN > 7 was prepared for sequencing using the RNAseq Ovation V2 kit (Nugen) or Smart-seq 4 low-abundance RNA kit (Takara) and sequenced on the Illumina HiSeq 4000 or Novaseq 2 platform in collaboration with Oxford Genomics Centre.
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bioRxiv - Microbiology 2020Quote: ... Total viral DNA was extracted using Qiagen viral DNA extraction kit (QIAamp DNA Mini Kit, Hilden, Germany) and DNA polymerase Tag (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... and indexing were performed as previously described using the Qiagen AllPrep DNA/RNA Micro Kit and the SMARTer Stranded Total RNA-Seq Kit v2 (Takara) (46 ...
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bioRxiv - Molecular Biology 2019Quote: ... Directional libraries were prepared using the Smarter Stranded Total RNA-Seq kit-Pico Input Mammalian kit following the manufacturer’s instructions (Clontech, 635005). The quality of all libraries was verified with the DNA-1000 kit (Agilent ...
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bioRxiv - Neuroscience 2019Quote: ... cDNA synthesis and library preparation was performed using the SMART-Seq v4 Ultra Low Input RNA Kit and Low Input Library Prep Kit v2 (Clontech), respectively ...
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bioRxiv - Neuroscience 2019Quote: ... cDNA synthesis and library preparation was performed using the SMART-Seq v4 Ultra Low Input RNA Kit and Low Input Library Prep Kit v2 (Clontech), respectively ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the manufacturer specifically states they should not occur in RNA-Seq reads if used in combination with the Nextera XT DNA library preparation kit (Appendix C in SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing User Manual, Takara Bio Inc ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA libraries were prepared with the Trio RNA-seq + UDI Library Preparation Kit (NuGEN) or the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Clontech) and sequenced on the MiSeq ...
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bioRxiv - Cancer Biology 2024Quote: ... The full-length cDNA of the human ZNRF3 was acquired from the human CRC DLD-1 cell line by RNA purification using NucleoSpin RNA II kit (Macherey Nagel) and reverse transcription RT-PCR using specific primers and Primescript RT reagent kit (TaKaRa). Then the ORF of the ZNRF3 short variant replaced RNF43 and was assembled into the C-terminal FLAG-tagged vector using Q5 Hot Start High-Fidelity DNA Polymerase (New England Biolabs ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted using a NucleoSpin RNA kit (Macherey Nagel) and reverse transcribed with the Primescript RT reagent kit (TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Sequencing libraries were prepared using “SMARTer Stranded Total RNA-seq Kit v3 – Pico Input Mammalian” kit (Takara Bio, Cat. # 634487) and the “SMARTer RNA Unique Dual Index Kit - 24U” (Takara Bio ...
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bioRxiv - Microbiology 2023Quote: ... Remaining DNA was removed using the TURBO DNA-free kit (Thermo-Scientific) and cDNA generated using the PrimeScript cDNA synthesis kit (Takara). qRT-PCR reactions were set up according to the manufacturer’s instructions (Alkali Scientific) ...
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bioRxiv - Genomics 2023Quote: ... A total of 3.5 μl ribo-depleted material was used for SMARTer (SMARTer PCR cDNA Synthesis Kit, catalog num. 634926 and Advantage 2 PCR kit, catalog num. 639207, Clontech-Takara) protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Isolated RNA was dissolved in 10 μl of DEPC-treated water and reverse-transcribed using a reverse transcription reagent kit (PrimeScript RT Reagent Kit with gDNA Eraser, Takara) and a thermal cycler (Mastercycler ...
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bioRxiv - Neuroscience 2024Quote: ... were purchased from Thermo Fisher Scientific. cDNA synthesis kit (cat no. 6110B) and real-time PCR kit (cat no. PN 4367659) were purchased from TAKARA. ECL reagent (cat no ...
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bioRxiv - Cancer Biology 2021Quote: ... a Prime Script RT kit (Takara, Tokyo, Japan) was adopted for the generation of complementary DNA ...
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bioRxiv - Neuroscience 2020Quote: We used the In-Fusion cloning kit (Takara) to amplify the cDNA of the long isoform of UNC-13 (UNC-13L ...
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bioRxiv - Microbiology 2021Quote: ... ligated together using Mighty Mix ligation kit (Takara), cloned into competent DH5α E ...
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bioRxiv - Developmental Biology 2020Quote: ... using the In-fusion HD cloning kit (Clontech) according to supplier’s instructions.
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bioRxiv - Genetics 2022Quote: ... GenTLE (from yeast) high recovery kit (Takara Bio). The genomic DNA concentration was measured using the Quantifluor® ONE dsDNA Dye System (Promega ...
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bioRxiv - Molecular Biology 2020Quote: ... and SMARTer DNA Unique Dual Index Kit (TAKARA) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2020Quote: The SMART® cDNA Library Construction Kit (Clontech) was used to reverse transcribe the zebrafish heart RNA ...
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bioRxiv - Molecular Biology 2021Quote: ... One-step PrimeScript miRNA cDNA Synthesis Kit (Takara) was applied for reverse transcription ...
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bioRxiv - Molecular Biology 2020Quote: ... or PrimeSTAR GXL DNA polymerase kit (Takara Co.). The amplicons were subcloned into the TOPO™ vector (Invitrogen ...
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bioRxiv - Genomics 2020Quote: ... and quantified using a Library Quantification Kit (Clontech), before sequencing on the Illumina HiSeq 1500 platform.
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bioRxiv - Immunology 2021Quote: ... Libraries were prepared using smRNA-seq kit (TAKARA) with the input of 1 µg µRNA for all three samples with positive and negative controls ...
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bioRxiv - Immunology 2021Quote: ... and the PrimeScriptTM RT reagent Kit (TaKaRa, Japan) as described in previous studies (Li et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... using In-Fusion HD Cloning Kit (Takara, 639649). Mutants for miR-320a sites were generated using site-directed mutagenesis by replacing the miR-320a seed match sequence with the HVS miR-HSUR4 seed match sequence ...
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bioRxiv - Microbiology 2019Quote: ... In-Fusion HD Cloning Kit (TaKara Bio, USA) was used for fast ...
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bioRxiv - Cell Biology 2019Quote: ... using the In-Fusion HD cloning kit (TaKaRa) according to manufacturers instructions ...
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bioRxiv - Genetics 2021Quote: ... cDNA first strand synthesis kit (TaKaRa RR036B, Japan), and One Step TB Green™ PrimeScript™ RT-PCR Kit II (SYBR Green ...
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bioRxiv - Cell Biology 2021Quote: ... A PrimeScript RT reagent kit (TaKaRa, Seoul, Korea) was used to reverse-transcribe mRNA into cDNA ...
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bioRxiv - Genomics 2021Quote: ... applying the In-Fusion HD #639649 kit (Clontech). The reactions were then pooled and purified with 1x Agencourt AMPureXP DNA beads #A63881 (Beckman Coulter ...
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bioRxiv - Plant Biology 2020Quote: ... the In-Fusion Advantage PCR Cloning Kit (Clontech) and Gateway Cloning Technology (Invitrogen ...
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bioRxiv - Bioengineering 2021Quote: ... with the NucleoSpin RNA kit (Takara-Bio, Japan). The purity and concentration of RNA were checked by NanoDrop and Urea PAGE electrophoresis ...
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The E3 ubiquitin-protein ligase MDM2 is a novel interactor of the von Hippel-Lindau tumor suppressorbioRxiv - Biochemistry 2020Quote: ... following the In-Fusion HD Cloning Kit (Clontech) protocol ...