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Citations for Takara Bio :
801 - 850 of 1168 citations for Mouse CDKL3 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... The guide for Cas9 was obtained by direct hybridization of the oligos and inserted in the same plasmid by Gibson assembly (In-Fusion HD cloning kit; Clontech) using the BtgZI sites.
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bioRxiv - Physiology 2020Quote: ... Amplified fragments were subcloned into the MluI and EcoRI sites of the pSEAP2-Basic reporter plasmid (Clontech, Mountain View, CA) and sequenced to confirm correct orientation (Eurofins Genomics ...
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bioRxiv - Microbiology 2021Quote: For human ectopic expression plasmids: Full length cDNA for each gene was amplified from a HeLa cDNA library (Takara Bio) and inserted into a pCDNA expression plasmid modified with either a C-terminal HA or N-terminal V5 tag under the human cytomegalovirus (CMV ...
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bioRxiv - Biochemistry 2021Quote: ... hCLPTM1L-FLAG-6His was amplified by PCR and cloned into pPB-tetON-BSD-tightFF plasmid by In-fusion cloning (Clontech). To construct pPB-tetON-Tmem16k-FLAG-6His-BSD-tightFF plasmid ...
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bioRxiv - Biochemistry 2021Quote: ... and K287A full-length aqMutLs were constructed by introducing the mutations into the pET-11a/aqmutL plasmid (47) using the PrimeSTAR mutagenesis procedure (Takara). The primer sets used for the construction of these mutant aqMutLs were 5’-TCGTAGAGGAACTCGTTGAGAATTCT-3’ and 5’-CGAGTTCCTCTACGACGTCAACAGGA-3’ ...
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bioRxiv - Systems Biology 2021Quote: ... Plasmids were constructed using either restriction digest and subsequent ligation or by using homology based cloning (In-Fusion, Takara 638911) and were sequence verified ...
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bioRxiv - Developmental Biology 2021Quote: ... the distal region of blastemas was co-injected and electroporated with a reporter plasmid (pEGFP-N2 or pRFP-N2; Clontech) together with the Tig1 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... respectively) and an mCherry coding sequence were amplified from the template plasmid by PCR using PrimeSTAR GLX DNA polymerase (TAKARA) along with the sense and antisense primer pair (5’-ATTACCTGGGGGTATCCCCTT-3’ and 5’-CACTCTTCTGGTTGTGGTTGC-3’) ...
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bioRxiv - Biochemistry 2021Quote: ... falciparum cDNA followed by Ligation Independent Cloning into HindIII/KpnI-cleaved plasmid pOPIN F (82) using the In-Fusion HD EcoDry Cloning Kit (Takara Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... 1 × 106 HEK293T cells were transfected with 4 μg donor and 2.5 μg pX330 Cas9 plasmid (CalPhos™ Mammalian Transfection kit, Clontech). After 72 h ...
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bioRxiv - Genetics 2021Quote: ... full-length cDNA encoding MELK was inserted between BamHI and NotI sites of three types of retroviral plasmids including the pRetroX-Tight-puro (Clontech), pRetroX-Tight-GFP-puro (containing N-terminal GFP ...
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bioRxiv - Cell Biology 2021Quote: ... The ZsGreen-ODC (ornithine decarboxylase) cells were previously generated by transfection of MelJuSo cells with the ZsProsensor-1 plasmid (BD Bioscience Clontech)20 ...
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bioRxiv - Genetics 2021Quote: ... The plasmid was linearized using AgeI and SalI restriction enzymes and inserts were cloned to the linearized plasmid in 17 reactions using the standard InFusion cloning (Clontech) protocol ...
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bioRxiv - Microbiology 2021Quote: ... the pLVX-ORF3-E plasmid was transfected into HEK-293T cells using the Lenti-X Packaging Single Shots kit (Takara). Lentiviral supernatants were harvested at 72 h post-transfection and filtered through a 0.22 μM membrane (Millipore ...
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bioRxiv - Immunology 2020Quote: ... according to methods outlined in previous studies.13,34 The ISG luciferase reporter plasmid pISRE-Luc vector was purchased from Clontech (USA). The SARS-CoV-2 M protein gene (NCBI access No ...
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Maize AFP1 confers antifungal activity by inhibiting chitin deacetylases from a broad range of fungibioRxiv - Microbiology 2021Quote: ... transformants containing the desired plasmids were screened on a selective dropout (SD) medium lacking tryptophan (W) and leucine (L) (Clontech). Protein interactions were assessed on SD selection medium lacking LW ...
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bioRxiv - Molecular Biology 2021Quote: The cDNA of eme1 and mus81 were subcloned into the pMBP-parallel1 and pCDFDuet-1 plasmids respectively using in-fusion (Takara) cloning system ...
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bioRxiv - Immunology 2021Quote: Variable heavy chain and light chain sequences were PCR amplified from yeast plasmid DNA and cloned into CMV/R IgG expression vectors using InFusion (Takara). Plasmid DNA for mammalian expression of the His-tagged SARS-CoV-2 RBD in a pCAGGS vector was kindly provided by Dr ...
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bioRxiv - Cell Biology 2021Quote: Transfection of Halo-tagged AnkB440 plasmids were conducted in HEK293T cells grown in 10 cm culture plates using the calcium phosphate transfection kit (Takara) and 8 µg of plasmid ...
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bioRxiv - Genetics 2019Quote: ... The entire open reading frame for the protein Reg1 and its derivative Reg1-P231L was expressed as a fusion to the Gal4 activation domain in the plasmid pACT2 (Clontech).
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bioRxiv - Genomics 2021Quote: ... of heat responsive MIR169 genes from tomato and Arabidopsis (Supplemental tables S7, S3) were cloned into the pABAI plasmid (Clontech) as baits ...
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bioRxiv - Biochemistry 2020Quote: ... siRNA resistant WRN transgenes containing a C-terminal 3xFLAG tag (designated WRNr) were synthesized and inserted into the lentiviral pLVX-IRES-puro plasmid vector (ClonTech) at GenScript ...
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bioRxiv - Molecular Biology 2021Quote: His6-mtRF1 was expressed in BL21 (DE3) that contained in addition the chaperone-encoding plasmid pG-KJE8 (Takara Bio Inc.). The cells were cultured in the presence of 5 ng/μl tetracycline and 0.5 mg/ml L-arabinose to induce chaperone expression ...
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bioRxiv - Neuroscience 2022Quote: ... 2016) with the design enabling two guide cassettes to be inserted into one SaCas9 plasmid by In-Fusion cloning (Takara). Guide sequences were designed using the online CRISPOR design tool (Haeussler et al ...
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bioRxiv - Cell Biology 2022Quote: ... All above sequences along with MBNL1 and MBNL2 isoforms imaged in primary neurons were cloned downstream of GFP in a pEGFP-C1 plasmid backbone under control of a CMV promoter (Takara). Full-length kinesin sequences were amplified from mouse cDNA and inserted upstream of an mScarlet-containing plasmid in a pcDNA3.1 backbone under the control of CMV promoter.
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bioRxiv - Biochemistry 2022Quote: ... The rituximab and cetuximab antibody variable sequences were generated by IDT and cloned into the variable regions of the VRC01 antibody plasmid vector (a generous gift from the Kim lab at Stanford) by using the In-Fusion cloning kit (Takara) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2022Quote: ... a polymerase chain reaction using 5’-TCTAGAGCTACTAACTTCAGCCTGCTG-3’ / 5’ - CGGTGGATCCCCTTCTTCC-3’ primers on Ibidi USA 60101 LifeAct-GFPtag2 plasmid was cloned with In-Fusion HD enzyme kit (Takara) into the pLVX vector (Clontech ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The cDNA of the RepNDK-RNA clone and the plasmids for all other RNA clones were subjected to digestion with Sma I (Takara) and in vitro transcription with T7 RNA polymerase (Takara) ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmids used for expression of fluorescent proteins in hTert-RPE1 cells are derivatives of the plasmid pEGFP-C1 and pmCherry-C1 (Clontech). Plasmids were analyzed by sequencing ...
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bioRxiv - Immunology 2022Quote: ... following manufacturer’s instructions with a 1.5:1.5:1 μg ratio of the SFG:Peq-Pam:RD114 DNA plasmids and concentrated using Retro-X™ Concentrator (Takara, Kusatsu, Japan) following manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... nls-edCitrineT9 was amplified from nlsABACUS1-2μ plasmid 14 and introduced into the pENTR221-f1-ABA-CUS2-truncation vectors using In-Fusion cloning (Takara) to generate pENTR-nlsABACUS2-100n and pENTR-nlsABACUS2-400n ...
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bioRxiv - Plant Biology 2022Quote: ... NLS and FLAG tag coding sequence were amplified from HBT_pcoCASphi_version1 and version2 plasmids and cloned into the digested vector together with PCR amplified pUB10 and Rbcs E9 terminator by TAKARA in-fusion HD cloning kit (cat639650) ...
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bioRxiv - Molecular Biology 2023Quote: Plasmid pCMV-GFP-Zfp106 was created by cloning the Zfp106 cDNA into the XhoI-EcoRI sites in pEGFP-C2 (Clontech). Plasmid pCMV-RFP-Zfp106 was created by replacing EGFP in plasmid pCMV-GFP-Zfp106 with NLS-TagRFP ...
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bioRxiv - Neuroscience 2022Quote: ... Stable cell line were generated as follows: A bicistronic expression vector was generated in the pTRE-tight-BI plasmid (Clontech) containing both human Nptx2 and human Nptxr cDNAs ...
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bioRxiv - Molecular Biology 2022Quote: ... Mammalian expression vector for Flag-EBNA1 contained B95-8 EBNA1 lacking the GA repeats (aa 101-324) under the control of CMV-3XFLAG promoter in a plasmid derived from pREP10 (Clontech) containing ...
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bioRxiv - Immunology 2022Quote: ... HEK 293T cells were seeded in a 6-well plate at a density of 6 × 105 cells/well and co-transfected with pSIN-siU6 – shSLFN11/ shSLFN12/ shSc (2 μg) along with plasmids pGP (1 μg, Takara) and pPE ampho (1 μg ...
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bioRxiv - Molecular Biology 2022Quote: ... The freshly extracted plasmids were transformed into yeast strain Y2H gold by using a yeast transformation kit (TaKaRa, Beijing, China) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... GFP-T42EM was created by cloning T42EM (Ding et al., 2006) into the HindIII and BamHI sites of the pEGFP-C1 plasmid (6084-1, Clontech). To verify tau protein expression ...
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bioRxiv - Microbiology 2023Quote: ... the pTAC promoter and coding sequence of the fluorescent markers were amplified from pLM426 derivatives (Supplemental table S3) and combined with the pBCC0029 recipient plasmid including a terminator sequence by In-Fusion HD from Takara. All plasmids for this study were purified using the NucleoSpin Plasmid kit (Macherey-Nagel) ...
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bioRxiv - Bioengineering 2023Quote: ... The recombinant plasmid of pGBKT7-GhERF105a/d and plasmid with empty vector were transformed respectively into strain AH109 yeast cells according to the manufacturer’s instructions (Clontech, USA). The transactivation activity was determined by the growth on SD/-Trp and SD/-Trp/-X-a-gal (5-bromo-4-chloro-3-indolyl-α-D-galactopyranoside ...
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bioRxiv - Synthetic Biology 2023Quote: ... each for a 50-µL reaction containing ∼30 fmol of EcoRI-XbaI digested pLVSIN-CMV-Pur backbone plasmid (Takara #6183), ∼300 fmol of the insert fragment ...
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bioRxiv - Cell Biology 2023Quote: AAV plasmids were prepared by subcloning the ORF to pAAV-CAG-DNP63A7 or using the In-Fusion HD Cloning kit (Clontech).
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bioRxiv - Molecular Biology 2023Quote: A yeast two-hybrid bait plasmid was constructed by cloning an AGO1 cDNA fragment encoding residues Q165-Y335 (NcGNAGO1) into the pGBKT7 vector (Clontech). Three libraries were used for the yeast two-hybrid screen of candidates interacting with AGO1 NcGN ...
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bioRxiv - Molecular Biology 2023Quote: ... and a termination signal (IDT) was co-transfected to A549 cells with a Cas9-expressing plasmid and a linear hygromycin marker (Clontech). Transfected cells were cultured in selective media with 100 μg/ml of hygromycin B for 2 weeks ...
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bioRxiv - Microbiology 2023Quote: ... The amplicon was then cloned into the EcoRI and NotI recognition site of the expression plasmid pCAG neo using HD Cloning Kit (TaKaRa). To construct deletion mutants of bovine hnRNPM ...
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bioRxiv - Microbiology 2022Quote: ... The Kpn I – BamHI env fragments from the pSVIIIenv plasmids were cloned into the corresponding sites of pE7SB-NL4-3 using Long Ligase (Takara) following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... Domain swapped mouse MR/GR constructs were constructed by amplification of the relevant fragments from donor plasmids and directional cloning using ligation-free In-Fusion technology (Clontech) in PCR-mediated linearized vectors ...
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bioRxiv - Plant Biology 2023Quote: ... was PCR amplified from the pET-28a plasmid bearing WT or individual six-point mutations (M1 to M6) using HS Primestar DNA polymerase (Takara) with forward and reverse primers bearing BamHI and XhoI ...
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bioRxiv - Biochemistry 2022Quote: ... a DNA fragment encoding residues 1-132 was amplified using the RPA expression plasmid as a template and CloneAmp Hi-Fi PCR master mix (Clontech, Takara) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The construct containing Y181G-coding point mutation was generated by inverse PCR of Zdhhc20WT plasmid using CloneAmp HiFi PCR Premix (Takara) and joining the ends of the PCR product using InFusion cloning.