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Citations for Takara Bio :
801 - 850 of 1131 citations for Human Caveolin 2 CAV2 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... Retrovirus was centrifuged for 2 hrs at 2560rcf at 320C onto wells pre-coated with RetroNectin (Takara). Wells were rinsed with PBS and CD8 T cells were added at 1×106 cells/mL in complete RPMI supplemented with 50 U/mL IL-2 and mouse T-activator Dynabeads (ThermoFisher ...
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bioRxiv - Genomics 2022Quote: ... and 2 µl of the reaction was transformed into 25 µl of Stellar chemically competent bacteria (Takara). Plasmid minipreps were performed using the NucleoSpin Plasmid Transfection-grade Mini kit (Macherey-Nagel ...
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bioRxiv - Immunology 2022Quote: ... The NTD domain was purified from clarified supernatants using 2 ml of cobalt resin (Takara Bio TALON), washing with 50 column volumes of 20 mM HEPES-HCl pH 8.0 and 150 mM NaCl and eluted with 600 mM imidazole ...
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bioRxiv - Genomics 2022Quote: ... and 2 µl of the reaction was transformed into 25 µl of Stellar chemically competent bacteria (Takara).
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bioRxiv - Cell Biology 2022Quote: ... full-length GIP-2 cDNA with a C-terminal V5-His6 tag was inserted into pColdI (TAKARA) and used to inoculate rabbits and rats ...
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bioRxiv - Cell Biology 2023Quote: ... Yeast 2-hybrid (Y2H) analysis was performed using the protocols described in the Yeast protocols handbook (Clontech, Protocol No ...
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bioRxiv - Biochemistry 2023Quote: ... The virus particles were further concentrated at 10X in volume using Lenti-X-concentrator (Clontech, PT4421-2) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Amplification was performed using the Advantage 2 Polymerase Mix (Clonetech, now Takara Bio USA, Mountain View, CA) and the following primers ...
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bioRxiv - Bioengineering 2024Quote: ... Viral genome number was quantified by real-time PCR with AAVpro Titration Kit Ver.2 (Takara, 6233) according to manufacturer’s instruction ...
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bioRxiv - Plant Biology 2019Quote: 6His-GST-CRK2cyto and 6His-MBP-RBOHD/C constructs for recombinant proteins were generated by using In-Fusion technology (Clontech). The coding regions of CRK2cyto (WT ...
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bioRxiv - Cell Biology 2020Quote: ... To detect the transgenic GFP-tagged Abl proteins we used anti-GFP (JL-8, 1:500 or 1:1000, Clontech). Anti-αTubulin (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... Then the homology arms and fluorescent protein sequences were assembled and cloned into the BamHI site of a pUC19 vector using Gibson assembly (Clontech). The primer sequences for the mGFP-EB1 ...
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bioRxiv - Developmental Biology 2020Quote: Doxycycline-inducible overexpression of HA-tagged proteins was achieved using the Lenti-X Tet-On 3G Inducible Expression System (Clontech) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... as described previously (Keith et al., 2012, Freund et al., 2016) with plasmids encoding green fluorescent protein (GFP) (pEGFPN1; Clontech), mouse Kif11 (Myers and Baas ...
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bioRxiv - Molecular Biology 2021Quote: Stable 3T3 cells (ATCC) overexpressing zDHHC3 or green fluorescent protein (GFP) as a control were generated using the pLVX lentiviral system (Clontech). Cells were labeled by stable isotope labeling with amino acids in cell culture (SILAC ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Genes of target prokaryotic proteins were amplified by PCR from corresponding genomic DNA and inserted into the pEGFP-C1 vector (Clontech). Mutated genes of prokaryotic proteins were obtained by PCR site-specific mutagenesis ...
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bioRxiv - Microbiology 2020Quote: ... Total protein was extracted from 2,000 midguts using the lysis buffer supplied in the Capturem IP & Co-IP kit (Takara, 635721). The extracted proteins were divided into two equal parts and used for immunoprecipitation (IP) ...
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bioRxiv - Biochemistry 2022Quote: ... The induction of His-and GST-fusion proteins and their subsequent purification using TALON® Metal Affinity Resin (Clontech Laboratories) and Glutathione SepharoseTM 4 Fast Flow beads (GE Healthcare) ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant His-tagged protein was purified from cell-free extracts using immobilised metal affinity chromatography (IMAC using Talon resin; Clontech) as described previously (34) ...
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bioRxiv - Microbiology 2022Quote: ... Virus production was quantified by determining the amount of the Gag p24 protein using an enzyme-linked immunosorbent (ELISA) assay (Innogenetics or TaKaRa). For production of HIV-1 Env-pseudotyped viruses in Jurkat cells ...
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bioRxiv - Plant Biology 2022Quote: ... The immunoprecipitated (RGA-GFP and RGAm2-GFP) and co-immunoprecipitated (IDD2-RFP) proteins were detected by western-blot with anti-GFP (JL8; Clontech) and anti- RFP (6G6 ...
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bioRxiv - Cancer Biology 2020Quote: ... the TET protein expression in each clone was checked by immunoblotting using TetR monoclonal antibody (Clone 9G9) (Clontech, cat#631131). In addition ...
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bioRxiv - Microbiology 2022Quote: ... Plasmid pTAP-AFAP was constructed by cloning the gene fragment encoding AFAP-streptavidin-binding peptide-3xFLAG tag fusion protein (AFAP-SBP-3xFLAG, shorted as AFAP-SF) into mammalian expression vector pIRESpuro3 (Takara). SBP is a peptide with the length of 38 amino acids (Wilson et al. ...
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bioRxiv - Molecular Biology 2019Quote: ... The cell debris was removed upon centrifugation and the proteins were purified from the supernatant by His-tag affinity chromatography using Ni-NTA agarose beads (Clontech). The bound proteins were washed with lysis buffer containing 10 mM imidazole and then eluted with lysis buffer containing 250 mM imidazole ...
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bioRxiv - Biochemistry 2019Quote: ... The cell debris was removed upon centrifugation in a SS34 rotor at 4 °C (27,000 × g for 15 min) and the proteins were purified from the supernatant by His-tag affinity chromatography using Ni-NTA agarose beads (Clontech). The bound proteins were washed with lysis buffer containing 25 mM imidazole and then eluted with lysis buffer containing 500 mM imidazole ...
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The Arabidopsis F-box protein FBW2 degrades AGO1 to avoid spurious loading of illegitimate small RNAbioRxiv - Cell Biology 2021Quote: ... Yeast transformation as well as yeast protein extraction were performed following the recommendations presented in the Yeast Protocol Handbook PT3024-1 (Clontech).
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bioRxiv - Biophysics 2020Quote: ... 6xHis-tagged protein fraction was incubated for thirty minutes at 4°C with Talon resin (Clontech Laboratories/Takara Bio USA) that had been previously equilibrated in lysis/extraction buffer ...
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bioRxiv - Biophysics 2020Quote: ... 6xHis-tagged protein fraction was incubated for thirty minutes at 4°C with Talon resin (Clontech Laboratories/Takara Bio USA) that had been previously equilibrated in lysis/extraction buffer ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant protein were purified to >90% electrophoretic purity by immobilized metal ion affinity chromatography using a cobalt-based matrix (Talon, Clontech) and eluted with imidazole as described previously24 ...
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bioRxiv - Molecular Biology 2020Quote: PJ69-4a strain of Saccharomyces cerevisiae was used for the expression of proteins of interest using the Matchmaker Gal4-based yeast two hybrid system (Clontech). Tested bait/prey combinations were co-transformed into PJ69-4a and cultivated at 28°C ...
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bioRxiv - Biochemistry 2019Quote: Hexa-histidine-tagged scaffolding protein (6 mg) was loaded on a 1 ml immobilized metal affinity chromatography column charged with cobalt (Clontech). Coat protein monomers (0.2 mg/ml ...
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bioRxiv - Microbiology 2021Quote: ... The pcDNA3.4 expression vector containing the sequence that encodes the His-tagged extracellular domain of the spike protein was transfected into Expi293 cells and the His-tagged spike protein produced in the culture supernatants was then purified with a Talon resin (Clontech).
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bioRxiv - Biophysics 2020Quote: pDNA_HaloTag/SNAP-tag/GFP vectors encoding each target protein (see Note 1),which were inserted by In-Fusion HD Cloning Kit (Clontech) or Seamless Ligation Cloning Extract (SLiCE ...
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bioRxiv - Cell Biology 2020Quote: ... generates a protein that undergoes spontaneous aggregation that reverses upon the addition of D/D solubilizer (catalogue# 635054; Takara Bio), a cell-permeant rapamycin analog that binds to the FM4 domains (see Fig 3e).
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bioRxiv - Immunology 2020Quote: ... For binding studies the 6xHis tag were removed from some Fabs by treatment with PreScission protease (MolBioTech; ThermoScientific) and the protein repurified on cobalt-nitrilotriacetic acid (Co-NTA) agarose (Clontech) followed by gel filtration chromatography on Superdex 200 (GE Healthcare ...
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bioRxiv - Molecular Biology 2019Quote: A DNA fragment containing CDS of each Hero protein and C-terminal FLAG and His tags was inserted into pCold I (Takara) by NEBuilder HiFi DNA Assembly Master Mix (NEB) ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmids used for expression of fluorescent proteins in hTert-RPE1 cells are derivatives of the plasmid pEGFP-C1 and pmCherry-C1 (Clontech). Plasmids were analyzed by sequencing ...
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bioRxiv - Cancer Biology 2022Quote: ... was modified to replace the puromycin-resistance gene with the enhanced green fluorescent protein (EGFP) gene using the In-Fusion Snap Assembly Master Mix (TaKaRa). Forty-eight hours after transfection ...
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bioRxiv - Microbiology 2022Quote: ... His-tagged AmiC was isolated from the protein sample using a TALON® metal affinity resin (Takara Bio USA, Inc) as previously described (51 ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 × 106 HEK293T cells were transfected with 7 μg of Env expressor and 1 μg of a green fluorescent protein (GFP) expressor (pIRES2-EGFP; Clontech) with the calcium phosphate method ...
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bioRxiv - Biophysics 2022Quote: ... These plasmids were designed by fusing the coding sequences of the respective proteins C-terminal (intracellularly) via a linker to mEGFP (called D0 / Donor only) or mCherry in the pIRESpuro2 vector (Clontech)32 (for more linker details see Supplementary Table 5) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The recombinant proteins were purified using a TALON Metal (Cobalt) Affinity Resin column (Clontech Laboratories, Inc., Palo Alto, CA, USA) and eluted with a linear gradient of imidazole (0–1,000 mM ...
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bioRxiv - Plant Biology 2023Quote: ... and the input and pull-down prey proteins were detected by immunoblot using anti-His (M201, Takara, 1:3000 dilution) and anti-GST (G018 ...
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bioRxiv - Plant Biology 2024Quote: The yeast two-hybrid assays were carried out using a mating-based split ubiquitin system (mbSUS) for membrane protein interactions as described “mbSUS tests protocol” (Clontech) (Obrdlik et al. ...
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bioRxiv - Plant Biology 2024Quote: The HIS3 and ADE2 reporter genes were employed to assess the protein interactions in the yeast strain Y2HGold (Takara Bio). Yeast transformation was carried out using the lithium acetate (LiAc)-mediated method ...
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bioRxiv - Genomics 2024Quote: ... The cells were infected with prepackaged lentiviral particles (constitutive reporter vector expressing tdTomato fluorescent protein gene driven by EF1a promoter (Takara) at MOI of 20 (Stock ...
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bioRxiv - Neuroscience 2024Quote: ... HEK293 cells in 24-well plates were transfected with a total of 0.9 μg of DNA vectors carrying genes encoding GluN1 and GluN2 subunits and green fluorescent protein (GFP; pQBI 25 vector; for identification of transfected cells; Takara) in a 1:1:1 ratio using 0.9 μl of PolyMag reagent (OZBiosciences ...
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bioRxiv - Biochemistry 2024Quote: ... Recombinant His-tagged protein was purified from cell free extracts in a single step using immobilized affinity chromatography (IMAC) with Talon resin (ClonTech) and dialyzed overnight into 20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2024Quote: ... pLysS or Rosetta pLysS cells and overexpressed proteins were purified using immobilized metal-ion affinity chromatography (IMAC) with TALON resin (Clontech) (Abad et al ...
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bioRxiv - Cell Biology 2020Quote: ... SLD3 and ESA1 ORF were PCR-amplified with Taq polymerase (MightyAmp ver. 2, Takara Bio Co., Otsu, Japan) and cloned into the BamHI sites of the Y2H DB vector pST1667 and the Y2H AD vector pST548 (Tanaka ...