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Citations for Takara Bio :
751 - 800 of 2043 citations for DOLICHOL FROM BOVINE HEART since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Single-stranded cDNA is synthesized from total RNA by cDNA synthesis kit (TAKARA) by priming at 65 ℃ and reverse transcription for 1 h at 42 ℃ in a 20 µl reaction mixture.
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bioRxiv - Neuroscience 2023Quote: ... DNA from two selected colonies was amplified using LA Taq DNA polymerase (TaKaRa) and the region between the homology arms was sequenced ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was isolated from cells using RNAiso Plus Regent (Takara, Cat# 9108) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was isolated from cells using RNAiso Plus Regent (Takara, Cat# 9108) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Total RNA from tissues and cells was extracted using TRIzol reagent (Takara, Japan) and were reversed transcribed into first-strand cDNA using the PrimeScript RT Reagent Kit (Takara ...
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bioRxiv - Developmental Biology 2023Quote: ... Plasmids were assembled from linear fragments using In-Fusion HD (Takara Bio, 638910) and amplified in Stellar chemically competent E ...
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bioRxiv - Neuroscience 2023Quote: ... The rest of the clones were linearized using restriction enzymes bought from Takara, using a standard procedure ...
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bioRxiv - Plant Biology 2023Quote: ... qRT-PCR was performed from cDNA using SYBR Green qPCR mix (TaKaRa, RR601A) following the manufacturer’s instructions using the LightCycler 480 system (Roche Diagnostics) ...
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bioRxiv - Immunology 2023Quote: RNA was isolated from cell pellets using Nucleospin RNA Plus XS kit (Takara), and first strand cDNA synthesis was performed using Superscript IV VILO Master Mix with ezDNAse (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: pTetO-ZsGreen (pTetO-ZsG) was obtained from Clontech (pmRi-ZsGreen; Cat. No. 631121) and all other pTetO promoters are copies of the PTet-14 promoter (Urlinger et al. ...
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bioRxiv - Neuroscience 2024Quote: The primary human fibroblasts (HFs) and the corresponding iPSCs were purchased from Takara Bio (Kusatsu ...
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bioRxiv - Neuroscience 2024Quote: The mammalian expression vector pBApo-CMV-Pur (cat. #3421) was purchased from Takara. We used Genescript to insert the 144 amino acid sequence for WT synuclein into the backbone and create 6 mutated strains ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted from liver organoids using RNAiso Plus reagent (TaKaRa, Japan) in combination with chloroform and isopropanol ...
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bioRxiv - Microbiology 2024Quote: ... The coding sequence of YFP was amplified from the plasmid pEYFP-N1 (Clontech) using an upstream primer (YFP_F ...
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bioRxiv - Immunology 2023Quote: miRNA was extracted from myotube-derived EVs using TRIzol reagent (Takara Biotechnology, Japan) according to the manufacturer’s instructions ...
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Molecular basis of proteolytic cleavage regulation by the extracellular matrix receptor dystroglycanbioRxiv - Biochemistry 2024Quote: ... All mutagenesis was done using instructions from In-Fusion HD Cloning Mix (Takara) unless noted below.
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bioRxiv - Neuroscience 2023Quote: ... Cell debris was filtered from the collected medium and Lenti-X Concentrator (Clontech) was then used to precipitate the lentivirus in the medium ...
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bioRxiv - Molecular Biology 2023Quote: ... pLenti MYC:CTRL vector was modified from pLenti MYC:PKR by InFusion cloning (Takara Bio). PKR K296R and PKR T451A were obtained by InFusion mutagenesis (Takara Bio ...
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bioRxiv - Biochemistry 2023Quote: ... SYBR Green real-time PCR Master Mix was purchased from Takara (Beijing, China). Other antibodies included anti-LDLR (ET1606 ...
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bioRxiv - Biochemistry 2023Quote: ... Lenti-X™ 293T cell line (human, female origin) were purchased from TaKaRa and used without further authentication ...
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bioRxiv - Molecular Biology 2022Quote: ... qPCR was performed in triplicate with TB green Premix Ex Taq from Takara and the Biorad CFX thermocycler ...
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bioRxiv - Cell Biology 2022Quote: ... Cytosolic GFP (pEGFP-N1) and mCherry (pmCherry-N1) vectors were purchased from Clontech. H2B-mCherry was a gift from Robert Benezra (Addgene plasmid #20972 ...
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bioRxiv - Biochemistry 2022Quote: ... Pyrobest™ DNA Polymerase and PrimeSTAR HS DNA Polymerase were purchased from TaKaRa Shuzo Co ...
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bioRxiv - Cell Biology 2022Quote: ... Mutagenesis of the ATGL clone was done using In-Fusion technology from Clontech Company (Catalog 639650 ...
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bioRxiv - Genomics 2022Quote: ... Total RNA pools from human brain (cat. no. 636530, Takara Bio USA, Inc), small intestine (cat ...
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bioRxiv - Immunology 2022Quote: ... Lentiviral particles were concentrated from clarified supernatants using Lenti-X Concentrator (Takara Bio). RAW 264.7 cells were transduced with the lentiviral particles and then selected with 4 μg/mL puromycin (Gibco) ...
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bioRxiv - Synthetic Biology 2022Quote: ... PrimeStar DNA polymerase and SapphireAmp* Fast PCR Master Mix were purchased from TaKaRa Bio ...
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bioRxiv - Molecular Biology 2022Quote: EGFP and TDP-43EGFP cDNAS were amplified from pEGFP-C1 (Clontech, 6084-1) and pEGFP-C1-TDP-43 (kind gift from Dr ...
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bioRxiv - Molecular Biology 2023Quote: ... Total RNA was extracted from tissues using NucleoSpin RNA (Takara Bio, Shiga, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Adeno-X Maxi Purification Kit and Adeno-X Rapid Titer Kit from Clontech were used for purification and titration ...
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bioRxiv - Cancer Biology 2023Quote: ... All constructs were cloned into pLVX-IRES-Puro (modified from pLVX vector (Clontech) by removal of the ZsGreen and replacement with a multiple cloning site and IRES-Puro ...
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bioRxiv - Cancer Biology 2023Quote: ... Adeno-X Maxi Purification Kit and Adeno-X Rapid Titer Kit from Clontech were used ...
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bioRxiv - Immunology 2023Quote: ... SMART MMLV reverse transcriptase (639524) and carrier DNA (630440) were purchased from Clontech, Takara Bio ...
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bioRxiv - Biochemistry 2023Quote: The retroviral titre calculation was done using the Retroviral titration kit from Takara Biosciences (Retro-X™ qRT-PCR Titration Kit 631453 ...
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bioRxiv - Neuroscience 2023Quote: ... and then the coding sequence of eGFP (sequences extracted from pIRES2-EGFP, Clontech). Hβ4StrepTagII-nAChR-IRES-mTurquoise ...
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bioRxiv - Plant Biology 2024Quote: ... PCR amplification was performed using Advantage® Genomic LA Polymerase Mix from TaKaRa Bio USA (Mountain View ...
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bioRxiv - Plant Biology 2024Quote: ... from plasmids pFny209 (see below) or pGFP-C1 (Clontech laboratories, Mountain View, CA), into the BpiI-digested modified pUC18 ...
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bioRxiv - Microbiology 2024Quote: Total RNA extracted from intestinal tissues by RNAiso Plus kit (TAKARA, Dalian, China) was reverse-transcripted using PrimeScript II 1st Strand cDNA Synthesis Kit (TAKARA ...
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bioRxiv - Neuroscience 2024Quote: ... the fluorescent proteins were amplified from pAM FLEX eGFP and pmCherry-C1 (Clontech) respectively ...
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bioRxiv - Neuroscience 2024Quote: ... Libraries were prepared from <=2.5ng input RNA using ¼ volume SmartSeqv4 technology (Takara Bio) and sequenced on NextSeq High Output flow cells ...
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bioRxiv - Molecular Biology 2024Quote: Complementary DNA (cDNA) for human EXT2 was amplified from cDNA library (Takara Bio); cDNAs for Sulf1 and Sulf2 were gifts from Steven Rosen (Addgene plasmid)34 ...
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bioRxiv - Neuroscience 2024Quote: ... Adenoviral titers were determined using the Adeno-X qPCR titration kit from Takara Bio USA (Mountain View ...
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bioRxiv - Neuroscience 2024Quote: ... Adenoviral titers were determined using the Adeno-X qPCR titration kit from Takara Bio USA (Mountain View ...
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bioRxiv - Neuroscience 2024Quote: ... AAV6 was extracted and purified from 293T using AAVpro purification kit (Takara, 6666) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: RNA was purified from transiently transfected HEK293-FT cells by NucleoSpin RNA (Takara) and the concentrations determined by Nanodrop One (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: Total RNA was extracted from Mm cells using the TRIzol Reagent (Takara, Japan). cDNA was synthesized using a PrimeScript reverse transcriptase reagent kit (Takara ...
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bioRxiv - Neuroscience 2024Quote: ... and GluA4 flip(Q) were amplified from mouse brain marathon-ready cDNA (Clontech) by PCR ...
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bioRxiv - Immunology 2024Quote: Total RNA was extracted from Treg cells using the RNAAiso Plus(9109; Takara) reagent according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... His-tagged protein was purified from the supernatant post centrifugation using Talon (TaKaRa) cobalt resin and eluted with 100 mM imidazole in buffer A.
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bioRxiv - Cell Biology 2024Quote: ... Total RNA was extracted from each sample using RNAiso Plus kit (9109; Takara). The cDNA was used for library construction using VAHTS Universal V8 RNA-seq Library Prep Kit for Illumina (NR605-01 ...