Labshake search
Citations for Takara Bio :
701 - 750 of 762 citations for Radiography Imaging system since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... cDNA was synthesized to a total of 500ng RNA in a 20 μL system using PrimeScript™ RT Reagent kit (TAKARA, #RR036A). q-PCR were performed on anlytik jena (qTOWER 2.0 ...
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bioRxiv - Neuroscience 2019Quote: ... Real-time monitoring of the PCR amplification reaction was performed using an ABI 7300 fast real time PCR system (CA, USA) and SYBR®Premix Ex TaqTM (Tli RNaseH Plus) (TaKaRa, Japan). The primers used were as follows ...
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bioRxiv - Zoology 2021Quote: ... and cDNA samples were used as templates for quantitative PCR using THUNDERBIRD SYBR qPCR mix (TOYOBO) on a Thermal Cycler Dice Real Time System (Takara Bio Inc.). The amount of target RNA was normalized to an endogenous control ribosomal protein 49 gene (rp49) ...
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bioRxiv - Plant Biology 2021Quote: ... The yeast strain AH109 was used for the yeast two-hybrid assay according to MatchmakerTM Two-Hybrid System 3 (Clontech, Palo Alto) and grown on YPD (full medium ...
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bioRxiv - Plant Biology 2021Quote: Yeast two-hybrid techniques were performed according to the yeast protocols handbook and the Matchmaker GAL4 Two-hybrid System 3 manual (both Clontech, Heidelberg, Germany) using the yeast reporter strains AH109 and Y187 ...
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bioRxiv - Plant Biology 2021Quote: ... Y2H screen was performed to screen the apple library according to the user manual of Matchmaker™ Gold Yeast Two Hybrid System (Clontech, Japan) by using Saccharomyces cerevisiae strain Y2H Gold.
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bioRxiv - Genetics 2020Quote: ... Verification of positive colonies was achieved by co-transformation of extracted bait and prey plasmids following the instruction of YeastMaker™ Yeast Transformation System 2 (Clontech, 630439) and by bait/prey plasmids with re-cloned cDNA.
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bioRxiv - Plant Biology 2019Quote: ... Real-time qPCR was performed in 48-well blocks with a StepOne Plus Real Time PCR System by using TaKaRa SYBR Premix Ex Taq II reagent kit (TaKaRa, Tokyo, Japan). All qRT-PCRs were performed in triplicate using total RNA samples extracted from three independent biological replicates ...
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bioRxiv - Immunology 2021Quote: ... and mNeonGreen were PCR-amplified and assembled into pPBbsr2 vector by using In-Fusion system (Takara Bio USA, Inc., Mountain View, CA). pCMV-mPBase (obtained from the Wellcome Trust Sanger Institute ...
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bioRxiv - Cell Biology 2022Quote: ... Matings between the Y2HGold and Y187 strains carrying the appropriate constructs were performed in 0.5 mL of 2X YPDA in the presence of the corresponding antibiotics following manufacturer’s instructions (Clontech’s Matchmaker Gold Yeast Two-Hybrid System, Clontech by Takara Bio, Mountain View, USA). Yeast cells were cultured at 30 ºC for 24 h and plated in SD-Trp/-Leu/X-alpha-Gal agar plates ...
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bioRxiv - Plant Biology 2022Quote: Direct protein-protein interaction was tested by Y2H technique according to the yeast protocols handbook and the Matchmaker GAL4 Two-hybrid System 3 manual (both Clontech, Heidelberg, Germany). Yeast strain Y190 was co-transformed with respective plasmids ...
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bioRxiv - Microbiology 2022Quote: ... The Apollo 324 NGS Library Prep System was used with the PrepX RNA-seq library preparation kit (Takara Bio, San Jose, CA). Sequencing was performed on Illumina NovaSeq 6000 platform (SP 100nt Lane ...
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bioRxiv - Plant Biology 2023Quote: A Y2H library screen was performed as outlined in the Matchmaker Gold “Mate and Plate” Yeast Two-Hybrid System (Takara Bio Inc.) using pGBKT7-CML13 as the bait in yeast strain AH109 ...
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bioRxiv - Cancer Biology 2023Quote: The MDM4 coding sequence (NM_002393.5) was cloned into Lenti-X™ Tet-One™ Inducible Expression System (Takara Bio, cat. no. 631847). The resulting plasmid was then subject to whole-plasmid sequencing for verification ...
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bioRxiv - Microbiology 2023Quote: ... DNA extracts were sent to Resistomap Oy (Helsinki, Finland) for HT-qPCR analysis using a SmartChip Real-time PCR system (TaKaRa Bio, Japan). The target genes included 27 ARGs and 5 MGEs (Table S3 ...
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bioRxiv - Microbiology 2023Quote: ... PK-15 cells were transfected with PX459-Ifnar1-k/o or PX459-Stat2-k/o plasmids using the TransIT-X2 Dynamic Delivery System (TaKaRa, Cat# V6100) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Inducible expression of a Nef-eGFP fusion protein was established in CEM-T4 cells using the Lenti-XTM Tet-On System (Clontech now Takara Bio USA) which places expression of Nef-eGFP under the control of a doxycycline-inducible promoter ...
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bioRxiv - Developmental Biology 2022Quote: ... Libraries were prepared using the directional PrepX RNA-seq library preparation protocol on the automated Apollo 324TM NGS Library Prep System (Takara Bio, CA), with a cDNA insert average size of ∼200bp.
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bioRxiv - Plant Biology 2024Quote: Yeast two-hybrid techniques were performed according to the yeast protocols handbook and the Matchmaker GAL4 Two-hybrid System 3 manual (both Clontech, Heidelberg, Germany) using the yeast reporter strains AH109 and Y187 ...
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bioRxiv - Plant Biology 2024Quote: The Y1H assays were conducted according to the manufacturer’s instructions (Matchmaker Gold Y1H Library Screening System; Clontech Laboratories, Mountain View, CA, USA). The pGADT7 empty vector with the pHis2 promoter and pGADT7-ZmHsf20 with the pHis2 empty vector were used as negative controls ...
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bioRxiv - Bioengineering 2024Quote: ... The plasmid pmCherry was generated by fusing the lacI-Ptrc inducible system and mCherry into the vector pBBR1MCS-5 using In-Fusion® Snap Assembly Master Mix (Takara Bio). All plasmids were extracted using QIAprep® Spin Miniprep Kit (Qiagen) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... transformed into the Saccharomyces cerevisiae Y187 strain (prey strain) was screened using the Matchmaker® Gold Yeast Two-Hybrid System (Takara Bio). For that purpose ...
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bioRxiv - Immunology 2024Quote: ... genes encoding the variable regions of C7 and C74 heavy chains were cloned into a pMN vector with a human CH1 domain and a C-terminal His-tag using In-Fusion cloning system (Takara Bio #639649). The full light chains of C7 and C74 were cloned into the pMN vector without any purification tag using the same method ...
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bioRxiv - Microbiology 2020Quote: ... and qRT-PCR was performed on StepOne Plus Real-time PCR system (Applied Biosystem) with TB Green Premix Ex Taq II (Takara Cat no. RR820A). Briefly ...
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bioRxiv - Synthetic Biology 2019Quote: ... Muc14a_5 and Muc14a_6 and Cas9 were tested for in vitro activity using the Guide-it sgRNA In Vitro Transcription and Screening System (Takara Bio USA, Inc.). Each kmer was transcribed and purified according to the In Vitro Transcription of sgRNA protocol ...
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bioRxiv - Microbiology 2020Quote: The coding sequence for MrvR was PCR amplified from 10/84 genomic DNA using primers optimized for subsequent cloning into the four supplied BIC Brevibacillus expression system pBIC plasmids (Takara cat. # HB300/HB310) in order to generate secreted ...
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bioRxiv - Systems Biology 2020Quote: ... The cell lines were cultured in IMDM medium (Quality Biological) supplemented with 10% (v/v) Tet System Approved fetal calf serum (Takara Bio/previously Clontech), 100 U/mL penicillin ...
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bioRxiv - Genetics 2019Quote: ... then converted to Illumina sequencing library using the PrepX RNA-seq library preparation protocol on the automated Apollo 324™ NGS Library Prep System (Takara Bio, CA). Basically ...
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bioRxiv - Cancer Biology 2021Quote: ... and PDIK1L cDNA (Horizon Discovery, Clone ID: 4828997) were cloned into LentiV_Neo vector using In-Fusion cloning system (Takara Bio; Cat. No. 121416). The N-terminus of STK35 cDNA (4–296 bp from translation start site ...
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bioRxiv - Plant Biology 2023Quote: ... These plasmids were transformed into the yeast strain AH109 for testing protein-protein interaction using the Matchmaker Gold system (Takara Bio, Kusatsu, Japan). Yeast transformation and growth were performed as described in [Pecher et al. ...
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bioRxiv - Physiology 2023Quote: ... The cDNA of each mouse gene was amplified and detected using THUNDERBIRD Next SYBR qPCR Mix (TOYOBO) and Thermal Cycler Dice Real Time System III (TAKARA BIO, Shiga, Japan). 18S ribosomal RNA was used for the reference gene ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1.25Lμg pVSV-G (second-generation lentivirus packaging system) into human embryonic kidney packaging cells GP2-293 (Clontech, Inc., Mountain View, CA, USA), using CalPhos™ Mammalian Transfection Kit (Clontech ...
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bioRxiv - Cell Biology 2023Quote: ... The hiPSC lines were differentiated into human pluripotent stem cell derived hepatocytes (hiPSC-HEP) by using the Cellartis iPS Cell to Hepatocyte Differentiation System (Cat. No. Y30055, Takara Bio Europe AB) according to the manufacturer’s recommendations.
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bioRxiv - Plant Biology 2023Quote: ... The yeast two-hybrid and one-to-one confirmation experiments were performed according to Matchmaker™ Gold Yeast Two-Hybrid System User Manual (Clontech, https://www.clontech.com/). Primers for vector construction were listed in Table S8.
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bioRxiv - Plant Biology 2023Quote: ... selections and LacZ reporter assays were made according to the Matchmaker Gold Yeast Two-Hybrid System user manual (Clontech; Mountain View, Ca, USA).
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bioRxiv - Microbiology 2023Quote: ... DNA extracts were sent to Resistomap Oy (Helsinki, Finland) for HT-qPCR analysis using a SmartChip Real-Time PCR system (TaKaRa Bio, Shiga, Japan). 10 ng DNA of each of the extracted samples were shipped to Resistomap for analysis ...
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bioRxiv - Immunology 2023Quote: ... M-IgA was expressed by transfecting a pair of alpha heavy and kappa light chain genes into FreeStyle CHO-S cells that were grown in a 24-well plate according to the manufacturer’s protocol (CHOgro High Yield Expression System, Takara Bio, Shiga, Japan). For large-scale antibody production ...
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bioRxiv - Evolutionary Biology 2024Quote: ... adhaerens yeast 2-hybrid cDNA library was constructed using the Make Your Own “Mate & PlateTM” Library System (Takara Bio USA, Mountain View, CA), using whole animal total RNA extracted from approximately 30 animals using a Nucleospin RNA Plus Mini Kit (Macherey-Nagel ...
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bioRxiv - Evolutionary Biology 2022Quote: A yeast two-hybrid prey cDNA library of Trichoplax whole animal RNA was prepared in the pGADT7- AD plasmid vector using the Make Your Own “Mate & Plate™” Library System (Takara Bio, California USA) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2019Quote: ... AAV9-G505R was generated by PCR mutagenesis of p5E18-VD2/9 using the In-Fusion Cloning HD Plus System (Takara Bio, Mountain View, CA). The pITR-CCR5-PGK-GFP vector genome plasmid contains AAV2 ITRs ...
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bioRxiv - Pathology 2019Quote: ... adenoviral vectors expressing GFP (Ad-GFP) and human PERK (Ad-PERK) were constructed using a one-step Adeno-X-ZsGreen adenoviral system (632267; Takara Bio, Mountain View, CA) following manufacturer’s instruction ...
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bioRxiv - Cell Biology 2022Quote: ... Matings between the Y2HGold and Y187 strains carrying the appropriate constructs were performed in 0.5 mL of 2X YPDA in the presence of the corresponding antibiotics following manufacturer’s instructions (Clontech’s Matchmaker Gold Yeast Two-Hybrid System, Clontech by Takara Bio, Mountain View, USA). Yeast cells were cultured at 30 ºC for 24 h and plated in SD-Trp/-Leu/X-alpha-Gal agar plates ...
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bioRxiv - Cell Biology 2022Quote: ... while pGADT7-CmVPS41s and pGADT7-T control prey plasmids were transformed into yeast strain Y187 following the Yeastmaker Yeast Transformation System 2 instructions (Clontech by Takara Bio, Mountain View, USA). The transformed cells were grown either in SD-Trp agar plates for Y2HGold or in SD-Leu agar plates for Y187 at 30 ºC for 3-5 days ...
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bioRxiv - Plant Biology 2022Quote: ... Real-time PCR was performed on an ABI Prism 7500 Fast Real-Time PCR System using the SYBR Premix Ex Taq kit (Takara Bio Inc., Shiga, Japan) according to the instructions ...
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bioRxiv - Neuroscience 2022Quote: Jacob-LMO4 interaction was reconfirmed using fusion vectors (bait vector pGBKT7, prey vector pGADT7) using MATCHMAKER Two-Hybrid System 3 (Takara Bio Europe/Clontech, France). Co-transformed yeasts were assayed for growth on quadruple drop-out medium (SD/–Ade/–His/–Leu/–Trp ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Inducible expression of a Nef-eGFP fusion protein was established in CEM-T4 cells using the Lenti-XTM Tet-On System (Clontech now Takara Bio USA) which places expression of Nef-eGFP under the control of a doxycycline-inducible promoter ...
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bioRxiv - Plant Biology 2023Quote: ... Bait (pDEST-GBKT7) or prey (pDEST-GADT7) vectors were transformed into Saccharomyces cerevisiae strains AH109 Gold and Y187 (Clontech, MATCHMAKER GAL4 Two-Hybrid System) respectively using a classical heat shock protocol (Gietz and Woods ...
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bioRxiv - Molecular Biology 2023Quote: ... tabaci adults was used for the synthesis of first strand cDNA utilizing the make your own mate & plate library system with CDS III adaptor primer and smart III oligo (template switching) as per manufacturer’s instructions (Takara Bio, San Jose, CA, USA). The first strand cDNA was used as template for long distance PCR with 5’ and 3’ primers ...
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bioRxiv - Neuroscience 2023Quote: ... with a HA tag and deletion mutants of Lag3 D1 domain with a myc tag were constructed by PCR with In-Fusion HD Cloning Plus systems (Takara; Bio Inc., Otsu, Japan) using the CloneAmp™ HiFi PCR Premix (a high-fidelity PCR polymerase included with all In-Fusion HD Cloning Plus Systems) ...
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bioRxiv - Genetics 2023Quote: ... The real-time PCR reaction system is as the followings: 10 µL of SYBR Premix Ex Taq™ (Takara Biom edical Technology, Beijing, China), 0.5 µM of each forward or reverses primer and 100-500 ng of DNA template ...