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Citations for Takara Bio :
651 - 700 of 980 citations for Recombinant Mouse PPAR gamma Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... HeLa cells stably expressing the tetracycline-controlled transactivator protein (Tet-ON, Clontech) were cultured in DMEM with 10% FBS and grown at 37°C with 5% CO2 ...
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bioRxiv - Plant Biology 2023Quote: ... the fusion protein was purified using TALON® Cobalt resin (Takara Bio). Following extensive washing in a buffer containing 20 mM HEPES (pH 7.5) ...
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Pyruvate kinase M2 regulates Japanese encephalitis virus replication by interacting with NS1 proteinbioRxiv - Microbiology 2024Quote: ... Pre-incubated samples were loaded onto the Capturem protein A columns (TaKaRa) and centrifuged at 1000g for 1 minute at room temperature ...
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bioRxiv - Genetics 2024Quote: Prey proteins were expressed from a modified pGADT7 AD vector (pGAD; Takara), which expresses a protein of interest fused to a GAL4 activation domain (AD ...
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bioRxiv - Immunology 2022Quote: ... The SMARTer Mouse TCR a/b Profiling Kit (Takara, Cat. No. 634403) was used to amplify both TCRα and TCRβ sequences ...
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bioRxiv - Cell Biology 2024Quote: ... The mouse PG cDNA was generated by PrimeSTAR Max DNA Polymerase (Takara) to skip 150 bp ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Sections were incubated overnight with mouse anti-STEM121 primary antibody (Takara, Y40410) and then developed with a DAB substrate kit (Cell Signaling ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation with mouse anti-GFP (1:500, Takara Bio, 632380), rabbit anti-mCherry (1:500 ...
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bioRxiv - Developmental Biology 2024Quote: ... in 1:200 dilution and mouse monoclonal anti-mCherry antibody (Takara, 632543) in a 1:400 dilution at 4°C on rocker ...
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bioRxiv - Genetics 2024Quote: ... total RNA was extracted from mouse tissues using TRIzol reagent (TAKARA, Japan). Whereafter 1μg of RNA was reverse-transcribed using the PrimeScript RT Reagent Kit (TAKARA ...
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bioRxiv - Biochemistry 2024Quote: ... Membrane was then incubated with 1:1000 mouse anti-His6 antibody (Clontech) for 15 mins ...
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bioRxiv - Developmental Biology 2024Quote: ... Total RNA was purified from mouse testis using NucleoSpin RNA (#740955.10; Takara). A cDNA library was synthesized from total RNA using ReverTra Ace qPCR RT Kit (#FSQ-101 ...
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bioRxiv - Systems Biology 2021Quote: ... the inducible fluorescent protein was induced by adding 1 μg/ml doxycycline (Clontech) alone or together with 100 nM rapamycin (Harveybio ...
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bioRxiv - Neuroscience 2021Quote: ... the fluorescent proteins were amplified from pAM FLEX eGFP and pmCherry-C1 (Clontech) respectively ...
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bioRxiv - Cell Biology 2021Quote: ... 6xHis tagged proteins were purified using Talon Co2+ affinity resin (Takara Bio, USA) and eluted with 150 mM Imidazole in the lysis buffer pH 7.8 ...
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bioRxiv - Microbiology 2021Quote: ... The protein bands were visualized using the Western BLoT Hyper HRP Substrate (TAKARA) and exposed using a Chemiluminescence Imaging System (Fusion Solo S ...
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bioRxiv - Plant Biology 2020Quote: ... Transferred proteins were first probed with anti-GFP (Takara Bio, 1:5,000 dilution) for 16 h ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins in the supernatant were purified by the affinity chromatography with TALON (Clontech) resin ...
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bioRxiv - Cell Biology 2020Quote: ... Shield-1 ligand for stabilization of DD-tagged proteins was purchased from Takara Bio (Cat # 632189 ...
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bioRxiv - Cell Biology 2023Quote: ... for GST-tagged protein or on TALON-Cobalt beads column (635507, Takara Bio) for 6XHIS-tagged protein as previously described [19,26] ...
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bioRxiv - Cell Biology 2023Quote: For mammalian expression of eGFP labelled proteins we used the pEGFP-C1 (Clontech) vector ...
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bioRxiv - Developmental Biology 2023Quote: ... which was induced for protein purification using Talon Metal Affinity Resin (Takara 635501). Two New Zealand white rabbits were injected at ten sites with 500μl of Freund’s Complete Adjuvant (Sigma-Aldrich F5881 ...
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bioRxiv - Plant Biology 2024Quote: ... The JMJ27-GFP fusion protein was detected using the anti-GFP (Takara: 632593) 1:2,000 (v ...
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bioRxiv - Microbiology 2024Quote: ... The fusion protein was affinity-purified using TALON metal affinity resin (Takara Biosciences) and eluted in buffer containing 50 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2023Quote: ... Monoclonal U-2OS cell lines stably expressing Tet-On 3G transactivator protein (Clontech), a tandem-dimeric MS2 hairpin binding protein tagged with eYFP (MS2CP-YFP) ...
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bioRxiv - Biochemistry 2023Quote: ... MA) and the protein was purified using Talon beads (Takara Bio Inc., Japan) following published protocols 37,38 ...
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bioRxiv - Neuroscience 2024Quote: ... the fluorescent proteins were amplified from pAM FLEX eGFP and pmCherry-C1 (Clontech) respectively ...
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bioRxiv - Developmental Biology 2024Quote: ... 100pg of sgRNA was injected along with 500 pg of spCAS9 protein (Takara). The F0 embryos were grown to adulthood and were then outcrossed with wild type to give rise to F1 animals ...
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bioRxiv - Biophysics 2024Quote: ... The solubilized protein was batch bound to Co-NTA resin (Takara Bio USA), washed with buffer A with 10 mM imidazole ...
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bioRxiv - Immunology 2021Quote: ... Blots were stained with the mouse α-GFP or α-mCherry (Clontech, EU) antibodies.
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bioRxiv - Neuroscience 2020Quote: Mouse Nwd1 cDNAs were subcloned into the pEGFP-C2 vector (Clontech Takara Bio) to express the Nwd1 protein fused with EGFP ...
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bioRxiv - Cell Biology 2020Quote: The mouse Tacc3 sequence was amplified by PrimeSTAR® Max DNA polymerase (TAKARA) using forward 5’-CTCCCCAGGGGGATCATGAGTCTGCATGTCTTAAAT-3’ and reverse 5’-GAGGTTGATTGTCGATCAGATCTTCTCCATCTTAG-3’ primers ...
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bioRxiv - Cell Biology 2022Quote: Mouse Itgb3 was cloned into the pLVX-EF1α-IRES-Puro vector (Takara Bio) per a previously described cloning strategy (Dave et al. ...
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bioRxiv - Immunology 2022Quote: ... or splenocytes from a naïve C57BL/6 mouse using NucleoSpin RNA Plus (Takara). RNA was reverse transcribed with SuperScript III RT and random primers (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... Immunoblots were probed with mouse anti-GFP (1:10,000 JL-8, Clontech #632380) and rabbit anti-β-actin (1:10,000 Cell Signaling #4967 ...
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bioRxiv - Microbiology 2020Quote: ... A mouse 7-day embryo cDNA Library (CATALOG No. 630478; Clontech Laboratories, Inc.) was used to identify host interaction proteins of Mtb PknG through yeast two-hybrid assay ...
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bioRxiv - Neuroscience 2020Quote: Mouse Nwd1 cDNAs were subcloned into the pEGFP-C2 vector (Clontech Takara Bio) to express the Nwd1 protein fused with EGFP ...
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bioRxiv - Microbiology 2023Quote: ... and mouse GAPDH transcripts as described previously 47 using SYBR green reagent (Takara) with primer pairs 1195/1196 ...
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bioRxiv - Physiology 2024Quote: ... (Cx43: 1:3000 custom-produced rabbit polyclonal; eGFP: 1:1000 mouse monoclonal (Clontech Laboratories Inc ...
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bioRxiv - Neuroscience 2024Quote: ... and GluA4 flip(Q) were amplified from mouse brain marathon-ready cDNA (Clontech) by PCR ...
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bioRxiv - Biochemistry 2021Quote: ... monoclonal antibody for green fluorescent protein (JL-8; Takara Bio, Kusatsu, Japan; recognizes VC), rabbit anti-GFP (D5.1 Cell Signaling Technology 2956S ...
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bioRxiv - Plant Biology 2022Quote: ... CITRINE-fusion proteins were detected with anti-GFP antibody (JL-8, Takara Bio Clontech, dilution ...
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bioRxiv - Microbiology 2021Quote: ... The protein was purified from the supernatant by TALON metal affinity resin (Clontech, #635606) and eluted with 150 mM imidazole ...
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bioRxiv - Microbiology 2021Quote: ... The protein concentration of each tissue was determined by BCA method (TaKaRa, Lot#A4401A). The tissue protein was used for SDS-PAGE electrophoresis at the same quality ...
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bioRxiv - Plant Biology 2021Quote: ... Transferred proteins were probed with anti-GFP (Takara Bio, 1:5000 dilution; Catalog # 632380) or anti-tubulin (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... target proteins were purified from cell lysate by Ni-iminodiacetic acid affinity chromatography (Clontech). However ...
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bioRxiv - Plant Biology 2021Quote: ... GFP-tagged proteins were detected using the anti-GFP antibody (JL-8, Clontech Takara) diluted 1:2000 (v/v ...
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bioRxiv - Plant Biology 2021Quote: ... GFP-tagged proteins were detected using the anti-GFP antibody (JL-8, Clontech Takara) diluted 1:2000 (v/v ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were first purified by affinity chromatography using TALON metal affinity resin (TaKaRa), and further purified via gel filtration chromatography on a Superdex 200 column (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: Protein extracts were analyzed by SDS-PAGE and immunoblotting using α-GFP (monoclonal, Takara), α-mCherry (polyclonal ...