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Citations for Takara Bio :
651 - 700 of 3141 citations for Probable U3 small nucleolar RNA associated protein 11 UTP11 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... GST-tagged protein was eluted with 6ml glutathione (10mg/ml, Takara) and the flow-through was collected ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the protein concentration was assessed and quantified using BCA (TaKaRa, Japanese). Immunoblotting was conducted on cell and tissue extracts (30 μg total protein/well ...
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bioRxiv - Microbiology 2022Quote: ... S-6P-NanoLuc proteins were released after HRV 3C protease (TaKaRa) treatment overnight at 4°C.
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bioRxiv - Biochemistry 2022Quote: ... The proteins were purified using TALON Metal Affinity Resin (Takara Bio) and eluted with a buffer containing 200 mM imidazole ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was purified by metal-affinity chromatography (Talon resin, Clontech), the affinity tag removed using tobacco etch virus (TEV ...
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bioRxiv - Cell Biology 2023Quote: ... His6-tagged proteins were purified with Talon metal affinity resin (Clontech) using the manufacturer’s protocol ...
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bioRxiv - Physiology 2022Quote: ... We used the Bradford Protein Assay Kit (TAKARA, T9310A, Shiga, Japan).
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bioRxiv - Neuroscience 2024Quote: ... Western blot stripping buffer and protein ladder were purchased from Takara bio (Kusatsu ...
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bioRxiv - Molecular Biology 2024Quote: ... The soluble proteins were purified using His60 Ni Superflow Resin (Takara). The purified proteins were further isolated using Superdex 75 16/600 (Cytiva) ...
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bioRxiv - Cell Biology 2021Quote: α-His primary antibody (1/10,000; Clontech) and HRP-conjugated goat anti-mouse IgG (H+L ...
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bioRxiv - Neuroscience 2021Quote: ... and 0.22 µg TaqStart Antibody (Clontech). 45-50 cycles of amplification were performed on a Bio-Rad C1000 thermocycler and the resulting product visualized with ethidium bromide on a 2% agarose gel.
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bioRxiv - Molecular Biology 2023Quote: ... anti GFP antibody (Clontech, lot. 1404005) and anti-H3 (Abcam ...
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bioRxiv - Bioengineering 2023Quote: ... Monoclonal Antibody (JL-8) (632381; Clontech) in 1:2,000 dilution ...
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bioRxiv - Plant Biology 2022Quote: ... The anti-GFP antibody (TaKaRa, 632381), the anti-LhcB2 antibody (Agrisera ...
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bioRxiv - Molecular Biology 2024Quote: ... an anti-mCherry antibody (#Z2496N, TaKaRa), or a horseradish peroxidase-conjugated anti-α-tubulin antibody (#HRP-66031 ...
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bioRxiv - Microbiology 2024Quote: ... anti-mCherry antibodies (Takara cat. # 632496) were initially mixed with Protein G attached dynabeads (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... First-strand cDNA was synthesized from 1 μg of total RNA using Oligo(dT) RNA to cDNA EcoDry Premix (Takara, Dalian, China). Primers used for qRT-PCR were designed using Primer 3Plus online software (http://bioinformatics.nl/cgi-bin/primer3plus ...
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bioRxiv - Microbiology 2020Quote: ... and subsequently cellular RNA was extracted at 6 hpi using a CellAmp Direct RNA Prep Kit (3732, Takara Bio Inc., Shiga, Japan). The qRT-PCR assays were performed to quantify the amount of SARS-CoV-2 RNA with QuantStudio 3 instrument (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... and the RNA library was prepared with the SMARTer Stranded Total RNA Sample Prep Kit - Low Input Mammalian (Takara catalog number 634861) and sequenced as described previously (29).
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bioRxiv - Neuroscience 2022Quote: Between 1-3 ng of the nuclear RNA was used in the SMART-seq v4 Ultra Low Input RNA Kit for Sequencing (Takara, cat # 634888) according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2020Quote: ... Cell supernatants were harvest 24 hours post-infection and RNA was extracted by a viral RNA extraction kit (Takara, Cat no. 9766). The number of viral copies was detected by real-time quantitative reverse transcription PCR (qRT-PCR ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were later processed for single cell RNA-seq using SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara Cat# 634894) as previously described41,45.
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bioRxiv - Neuroscience 2022Quote: ... double-stranded cDNA whole transcriptome library was synthesized from 1µg of total RNA with Takara Bio’s SMARTer Stranded Total RNA Sample Prep Kit – HI Mammalian (Takara Bio, Cat. No. 634876) and SMARTer RNA Unique Dual Index Kit (Takara Bio ...
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bioRxiv - Microbiology 2021Quote: ... RNA-Seq library preparation was performed using the SMARTer® Stranded Total RNA-Seq kit – Pico Input Mammalian (Takara Bio USA, Inc) with the RNA input ranging from 7 to 10 ng of starting material.
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bioRxiv - Microbiology 2021Quote: ... RNA was extracted with an Axygen RNA purification kit (Capital Scientific) and the first DNA was synthesized from RNA using a PrimeScript RT regent kit (Takara, Shiga, Japan). Actin-coding gene (KP341672 ...
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bioRxiv - Genomics 2021Quote: Paired-end RNA-seq libraries were constructed from >747 pg of RNA using the SMART-Seq v4 ULTRA Low Input RNA Kit for Sequencing (Takara, cat. 634891) and NexteraXT kits (Illumina ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 µg of high-quality total RNA (RNA integrity number > 8) was fragmented in the presence of Mg2+ in SMARTScribe Reverse Transcriptase buffer (Clontech, Cat. 639537), and mRNA fragments with a poly(A ...
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bioRxiv - Developmental Biology 2023Quote: ... and libraries were prepared as described previously with the following modifications: Libraries were prepared using the SMART-seq v4 Plus/ultra-low RNA input stranded total RNA-seq kit (Takara Bio Inc.) and sequenced on the NextSeq 1000/2000 (100 cycles ...
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bioRxiv - Genetics 2023Quote: ... RNA from 2 retinae of a mouse was extracted using the NucleoSpin® RNA kits (Takara Bio USA, Inc., San Jose, CA). RNA sample concentration and quality was determined with NanoDrop Oneᶜ Microvolume UV-Vis Spectrophotometers (ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... cDNA library was generated from 2 nanograms of total RNA using Smart-Seq V4 Ultra Low Input RNA Kit (Takara catalog#: 634894). 150 picograms of cDNA was used to make sequencing libraries by Nextera XT DNA Sample Preparation Kit (Illumina catalog# ...
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bioRxiv - Cancer Biology 2022Quote: ... For reverse transcription 2 µg RNA were translated into cDNA using the “RNA to cDNA EcoDry” Kit (Takara Bio USA, Kusatsu, Japan) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... 5 ng of total RNA was amplified and converted to cDNA using the SMART-Seq v4 Ultra Low Input RNA kit (Clontech Ref. 634889). Afterwards ...
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bioRxiv - Genomics 2022Quote: We prepared stranded RNA-Seq libraries for each plasma fraction using Clontech SMARTer stranded total RNA-seq kit v2-pico input mammalian (Takara Bio, 634414) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The fragmented RNA was used to produce barcoded cDNA sequencing libraries using the SMARTer® Universal Low Input RNA Kit (Takara, 634938). Short-read libraries were sequenced on Illumina NovaSeq S4 (paired-end 2×150) ...
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bioRxiv - Cell Biology 2023Quote: ... Libraries were prepared from total RNA using the Smarter® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Takara Bio 634411) and sequenced on a Novaseq 6000 ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA libraries from 5 ng total RNA were prepared using the SMARTer® Stranded Total RNA-Seq Kit (Takara Bio USA, #635005), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Purified total RNA was used for quantification of viral RNA with one-step PrimeScript III RT-qPCR mix (RR600A, Takara, Kyoto, Japan) and a CFX96 Real-Time PCR system (Bio-Rad ...
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bioRxiv - Plant Biology 2024Quote: ... about 1µg to 5µg total RNA was used for first-strand synthesis using RNA to cDNA EcoDry Premix with Oligo(dT) (Takara Bio Inc., Japan), by following the manufacturer’s recommendations ...
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bioRxiv - Physiology 2024Quote: ... and 5 µg of total RNA was used for synthesizing complementary DNA (cDNA) using an RNA to cDNA EcoDry™ Premix Kit (Clontech, USA). Quantitative PCR (qPCR ...
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bioRxiv - Developmental Biology 2024Quote: Libraries for RNA sequencing (RNA-seq) from sorted mammary cells were generated using SMARTer stranded total RNA-seq kit V2 – Pico input mammalian (TaKaRa bio, 634414) according to the manufacturer’s instructions using 13 cycles of amplification ...
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bioRxiv - Microbiology 2020Quote: ... Western blotting was performed using standard procedures with the following primary antibodies: JL-8 monoclonal antibody (Takara) for GFP variants ...
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bioRxiv - Microbiology 2023Quote: ... Standard immunoblotting procedures were performed using a primary antibody against GFP (mouse monoclonal JL-8 antibody, Takara) and a secondary antibody goat anti-mouse IgG-peroxidase antibody (Sigma) ...
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bioRxiv - Immunology 2023Quote: Primary antibodies used for imaging include Living Colors anti-DsRed Rabbit Polyclonal Pan Antibody (1:500; TaKaRa), Chicken Polyclonal anti-GFP (1:300 ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was extracted by RNAiso Plus (Takara, 9109) and purified with the guanidine isothiocyanate - phenol chloroform ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Total RNA was isolated using TRIzol reagent (Takara, Japan). Total RNA quantification was performed by NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2020Quote: ... SMART-Seq v4 Ultra Low input RNA kit (Clontech) was used to amplify cDNA which was subsequently sheared resulting in 200-500 bp fragments ...
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bioRxiv - Molecular Biology 2021Quote: ... Total RNA was extracted using RNAiso Plus (Takara Bio) according to the user manual ...
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bioRxiv - Cancer Biology 2020Quote: ... Human total RNA from different normal tissues from Clontech (Human Total RNA Master Panel II,Cat# ...
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bioRxiv - Zoology 2020Quote: ... MiniBEST Universal RNA Extraction Kit was purchased from TaKaRA, iScript™ gDNA Clear Synthesis Kit was purchased from Bio-Rad.
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bioRxiv - Microbiology 2021Quote: Total RNAs were extracted using RNAiso Plus (Takara Bio). The PrimeScript cDNA synthesis kits (Takara Bio ...