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651 - 700 of 2207 citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020

• A screen for Twist-interacting proteins identifies Twinstar as a regulator of muscle development during embryogenesis

  Mridula Balakrishnan, et al., bioRxiv - Developmental Biology 2021

  Quote: ... rabbit anti-dsRed (1:400, Clontech), and mouse anti-GFP (1:200 ...
• Modeling the marmoset brain using embryonic stem cell-derived cerebral assembloids

  Tomoki Kodera, et al., bioRxiv - Neuroscience 2023

  Quote: ... anti-FOXG1 (1:500, rabbit; Takara), anti-MAP2 (1:500 ...
• Schlafen 12 restricts HIV-1 latency reversal by a codon-usage dependent post-transcriptional block in CD4+ T cells

  Mie Kobayashi-Ishihara, et al., bioRxiv - Immunology 2022

  Quote: ... and pPE ampho (1 μg, Takara) using 12 μl of Lipofectamine 2000 (ThermoFisher ...
• Pathways controlling neurotoxicity and proteostasis in mitochondrial complex I deficiency

  Vanitha Nithianadam, et al., bioRxiv - Neuroscience 2024

  Quote: ... GFP (1:1,000, JL-8, Clontech), GABARAP/Atg8a (1:2,000 ...
• Functional and long-lived melanocytes from human pluripotent stem cells with transient ectopic expression of JMJD3

  Chie Kobori, et al., bioRxiv - Cell Biology 2023

  Quote: ... 1 μg/ml of puromycin (Takara) was added to the medium only for two days.
• Self-organization, quality control, and preclinical studies of human iPSC-derived retinal sheets for tissue-transplantation therapy

  Kenji Watari, et al., bioRxiv - Neuroscience 2023

  Quote: ... anti-Crx (rabbit; 1:200; Takara), anti-Recoverin (rabbit ...
• Optimal trade-off control in machine learning-based library design, with application to adeno-associated virus (AAV) for gene therapy

  Danqing Zhu, et al., bioRxiv - Bioengineering 2022

  Quote: ... rabbit dsRed (1:250, Takara, 632496), and DAPI ...
• Spatial and temporal transcriptomics of SHH-medulloblastoma with chromothripsis identifies multiple genetic clones that resist to treatment and lead to relapse

  Ilia Kats, et al., bioRxiv - Cancer Biology 2023

  Quote: ... For STEM121 (1:5000, Takara, #Y40410) and Ki67 (1:100 ...
• The SOCE system is critical for membrane bleb formation to drive avian primordial germ cell migration

  Mizuki Morita, et al., bioRxiv - Developmental Biology 2023

  Quote: ... 1 μg/ml Doxycycline (Dox) (Clontech) was added into PGC-cultured FAcs medium 24 hours before the injection ...
• VCF1 is an unconventional p97/VCP cofactor promoting recognition of ubiquitylated p97-UFD1-NPL4 substrates

  Ann Schirin Mirsanaye, et al., bioRxiv - Molecular Biology 2023

  Quote: ... His6 (631212, Clontech, mouse, 1:500); NPL4 (sc-365796 ...
• Ser149 is another potential 14-3-3 ε binding site of Cdc25B in the G2/M transition of mouse fertilized eggs

  Wen-Ning He, et al., bioRxiv - Cell Biology 2023

  Quote: ... rabbit anti-Myc (1:1000) (Clontech); rabbit anti-HA (1:800 ...
• eQTL mapping in transgenic alpha-synuclein carrying Caenorhabditis elegans recombinant inbred line

  Yuqing Huang, et al., bioRxiv - Genomics 2023

  Quote: ... 1 Unit ExTaq (Takara, catalog #RR001A), 0.2 µM primer 1 ...
• Tbx5 overexpression in embryoid bodies increases TAK1 expression but does not enhance the differentiation of sinoatrial node cardiomyocytes

  Yunkai Dai, et al., bioRxiv - Developmental Biology 2023

  Quote: ... DsRed (Clontech, cat#632392, 1:500), GFP (Thermo ...
• A rapid and bidirectional reporter of neural activity reveals neural correlates of social behaviors in Drosophila

  Moise Bonheur, et al., bioRxiv - Neuroscience 2023

  Quote: ... rabbit anti-DsRed (1:1000, Clontech), mAb anti-Bruchpilot (nc82 ...
• Quantitative proteomics and phosphoproteomics of PPP2R5D variants reveal deregulation of RPS6 phosphorylation through converging signaling cascades

  KA Smolen, et al., bioRxiv - Cell Biology 2023

  Quote: HEK293 (Clontech #C3003-1; lot #7030396) cell lines were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) ...
• Custom-engineered hydrogels for delivery of human iPSC-derived neurons into the injured cervical spinal cord

  V.M. Doulames, et al., bioRxiv - Bioengineering 2023

  Quote: ... and Stem123 (Takara Y40420; 1:500). The following secondary antibodies were used at a 1:800 dilution ...
• Sclerostin modulates the degree of mineralization and the stiffness profile of the fibrocartilaginous enthesis for mechanical tissue integrity

  Shinsei Yambe, et al., bioRxiv - Developmental Biology 2023

  Quote: ... anti-Ocn (Takara, M173; 1:800), anti-CD31 (BD ...
• Decryption of sequence, structure, and functional features of SINE repeat elements in SINEUP non-coding RNA–mediated post-transcriptional gene regulation

  Harshita Sharma, et al., bioRxiv - Molecular Biology 2023

  Quote: ... 1 µL of RNaseH (TaKaRa, 2150A), and 1 µL of RNase cocktail (Invitrogen ...
• The Bicaudal-D/Egalitarian complex defines the specificity of cargo transport by Dynein

  Frederick C. Baker, et al., bioRxiv - Cell Biology 2023

  Quote: ... mouse anti-GFP (Clontech,1:6,000), mouse anti-FLAG (Millipore-Sigma ...
• The yolk sac vasculature in early avian embryo provides a novel model for the analysis of cancer extravasation

  Mizuki Morita, et al., bioRxiv - Cancer Biology 2024

  Quote: ... 1 μg/ml Doxycycline (Dox) (Clontech) was added to the cultured cells’ medium 24 hours before injection ...
• Compartmentalized Cytoplasmic Flows Direct Protein Transport to the Cell’s Leading Edge

  Catherine G. Galbraith, et al., bioRxiv - Cell Biology 2024

  Quote: EGFP-Actin (Clontech cat #6116-1). Apple ...
• Sexual Failure Decreases Sweet Taste Perception in Male Drosophila via Dopaminergic Signaling

  Gaohang Wang, Wei Qi, Rui Huang, Liming Wang, bioRxiv - Neuroscience 2024

  Quote: ... rabbit anti-DsRed (1:1000, ClonTech), Alexa Fluor 488 goat anti-rabbit (1:1000 ...
• Glia multitask to compensate for neighboring glial cell dysfunction

  Allison N. Beachum, et al., bioRxiv - Neuroscience 2024

  Quote: ... rabbit anti-DsRed (1:1000; Clontech) rat anti-Elav (1:100 ...
• A Brain Reward Circuit Inhibited By Next-Generation Weight Loss Drugs

  Elizabeth N. Godschall, et al., bioRxiv - Neuroscience 2024

  Quote: ... anti-DsRed (rabbit, 1:1000; Takara Bio ...
• Genetically-encoded markers for confocal visualization of single dense core vesicles

  Junwei Yu, et al., bioRxiv - Neuroscience 2024

  Quote: ... rabbit anti-RFP (1:200; Takara), mouse anti-GFP (1:200 ...
• The phosphorylation of Pak1 by Erk1/2 for cell migration requires Arl4D acting as a scaffolding protein

  Ting-Wei Chang, et al., bioRxiv - Cell Biology 2024

  Quote: ... anti-His (1:5000, #631212, Takara), anti-Erk1/2 (1:2000 ...
• Replication-dependent histone (Repli-Histo) labeling dissects the physical properties of euchromatin/heterochromatin in living human cells

  Katsuhiko Minami, et al., bioRxiv - Cell Biology 2024

  Quote: ... aprotinin (1 µg/ml) (T010A; TaKaRa), and 0.1 mM phenylmethylsulfonyl fluoride (PMSF ...
• The Relationship between Birth Timing, Circuit Wiring, and Physiological Response Properties of Cerebellar Granule Cells

  S. Andrew Shuster, et al., bioRxiv - Neuroscience 2021

  Quote: ... and incubated in primary antibody (1:1,000 mouse anti-calbindin, Sigma; 1:500 rabbit anti-DsRed, Clontech; 1:1,000 guinea pig anti-vGluT1 ...
• Identification of the main barriers to Ku accumulation in chromatin

  Madeleine Bossaert, et al., bioRxiv - Molecular Biology 2024

  Quote: ... 4.9 μg DNA was transfected per dish containing a 1:1 molar ratio of pmCherry-N1 (Clontech) and of the pUC18-GFP or linear GFP substrate ...
• Compartmentalization and synergy of osteoblasts drive bone formation in the regenerating fin

  N Cudak, et al., bioRxiv - Developmental Biology 2023

  Quote: ... primary antibodies (chicken anti-GFP, Abcam, ab13970, 1:2000 and rabbit anti-DsRed, Clontech, 632496, 1:200) were incubated overnight at 4°C ...
• Evolution of resistance to Irinotecan in cancer cells involves generation of topoisomerase-guided mutations in non-coding genome that reduce the chances of DNA breaks

  Santosh Kumar, et al., bioRxiv - Cancer Biology 2023

  Quote: ... 1-st PCR (PCR 1) was performed using Titanium Taq DNA Polymerase (# 639209, Takara Bio, CA, USA). Separation of the PCR products from primers and gel purification was done by QIAquick PCR & Gel Cleanup Kit (Qiagen ...
• Flap endonuclease 1 repairs DNA-protein crosslinks via ADP-ribosylation

  Yilun Sun, et al., bioRxiv - Molecular Biology 2023

  Quote: ... pLKO.1-shFEN1 or pLKO.1 control vector was simultaneously transfected into LentiX293T (Cat# 632180 Clontech, Japan) with packaging plasmid ...
• Synapse specific and plasticity-regulated AMPAR mobility tunes synaptic integration

  Agata Nowacka, et al., bioRxiv - Neuroscience 2024

  Quote: ... was purchased from Clontech (Clontech; #P3070-5). Plasmids were prepared using the ZymoPURE Plasmid MaxiPrep Kit (Zymo Research ...
• The UBR box E3 ligases Poe and Hyd are required for efficient Pericentrin degradation

  Ramya Varadarajan, et al., bioRxiv - Developmental Biology 2022

  Quote: ... Primary antibodies were anti-PLP (N-terminus; 1:5000, Rogers, 2008) anti-GFP (JL8; 1:2000 – 5000; Clontech); anti-alpha-Tubulin (DM1A ...
• Social deficits induced by pervasive environmental stressors are prevented by microbial or dopaminergic modulation

  Caroline J. Smith, et al., bioRxiv - Neuroscience 2022

  Quote: ... sections were incubated with primary antibody (anti-Th [mouse] 1:1500, Immunostar; anti-dsRed [rabbit] 1:1500, Clontech) overnight at RT shaking ...
• Zebrafish Polycomb repressive complex-2 critical roles are largely Ezh2- over Ezh1-driven and concentrate during early embryogenesis

  Gabriel A. Yette, Scott Stewart, Kryn Stankunas, bioRxiv - Developmental Biology 2021

  Quote: ... Samples were incubated with chicken anti-GFP 1:1000 (Aves) and rabbit anti-DsRed 1:500 (Clontech/Takara) antibodies diluted in blocking solution overnight at 4°C ...
• Zebrafish Polycomb repressive complex-2 critical roles are largely Ezh2- over Ezh1-driven and concentrate during early embryogenesis

  Gabriel A. Yette, Scott Stewart, Kryn Stankunas, bioRxiv - Developmental Biology 2021

  Quote: ... Samples were incubated with chicken anti-GFP 1:1000 (Aves) and rabbit anti-DsRed 1:500 (Clontech/Takara) antibodies diluted in blocking solution overnight at 4°C ...
• Telomeric DNA breaks in human induced pluripotent stem cells trigger ATR-mediated arrest and telomerase-independent telomere length maintenance

  Katrina N Estep, et al., bioRxiv - Cell Biology 2023

  Quote: ... Cells were then induced for the indicated amount of time with 1 μM Shield-1 ligand (Takara bio) to stabilize the TRF1-FokI protein and 1 μM 4-OHT (Cayman Chemical ...
• Arabidopsis ABCC4 encodes a cytokinin efflux transporter and is involved in root system development

  Takuya Uragami, et al., bioRxiv - Plant Biology 2024

  Quote: ... Transformed yeast cells were grown under selective conditions in a minimal medium (46.7 g L-1 Minimal SD Agar Base [TaKaRa], 0.78 g L-1 uracil dropout supplement [TaKaRa]). Cytokinin transport assays were performed according to the method described by Zhao et al ...
• Defining totipotency using criteria of increasing stringency

  Eszter Posfai, et al., bioRxiv - Cell Biology 2020

  Quote: ... mouse anti-mCherry 1:500 (632543, Clontech); chicken anti-Gfp 1:500 (ab13970 ...
• R7 photoreceptor axon targeting depends on the relative levels of lost and found expression in R7 and its synaptic partners

  Jessica Douthit, et al., bioRxiv - Developmental Biology 2021

  Quote: ... rabbit anti- dsRed (1:500; Takara Bio), guinea pig anti-Loaf (1:400 ...
• Dynamic adult tracheal plasticity drives stem cell adaptation to changes in intestinal homeostasis

  Jessica Perochon, et al., bioRxiv - Developmental Biology 2021

  Quote: ... rabbit anti-DsRed 1:1000 (Clontech, #632496), mouse anti-Arm 1:3 (Hybridoma Bank ...
• Expression of a Barhl1a reporter in subsets of retinal ganglion cells and commissural neurons of the developing zebrafish brain

  Shahad Albadri, et al., bioRxiv - Developmental Biology 2020

  Quote: ... rabbit anti-DsRed (Clontech, 632496, 1:200), mouse anti-Tubulin (Sigma T5168 ...
• Cortical wiring by synapse-specific control of local protein synthesis

  Clémence Bernard, et al., bioRxiv - Neuroscience 2021

  Quote: ... rabbit anti-dsRed (1:500, Clontech #632496), goat anti-rat 647 (1:400 ...
• Novel System to Monitor In Vivo Neural Graft Activity After Spinal Cord Injury

  Kentaro Ago, et al., bioRxiv - Neuroscience 2021

  Quote: ... and STEM121 (msIgG1, 1:200, Takara Bio). The nuclei were stained with Hoechst 33258 (10 µg/ml ...
• A visual pathway for skylight polarization processing in Drosophila

  Ben J. Hardcastle, et al., bioRxiv - Neuroscience 2020

  Quote: ... Rabbit anti-DsRed (1:1000, 632496, Clontech); rabbit anti-HA (1:300 ...
• Production and characterization of virus-free, CRISPR-CAR T cells capable of inducing solid tumor regression

  Katherine P. Mueller, et al., bioRxiv - Bioengineering 2021

  Quote: ... 1/3 volume Retro-X concentrator (Takara) was added ...
• Paip2 associates with PABPC1 on mRNA, and may facilitate PABPC1 dissociation from mRNA upon deadenylation

  Jingwei Xie, et al., bioRxiv - Biochemistry 2021

  Quote: ... and anti-GFP (Clontech 632381 1:2000). Membrane was then washed three times in 1x TBST and incubated with goat-anti-rabbit (Jackson ImmunoResearch 111-035-046 1:5000 ...
• Loss of PABPC1 is compensated by elevated PABPC4 and correlates with transcriptome changes

  Jingwei Xie, Xiaoyu Wei, Yu Chen, bioRxiv - Biochemistry 2021

  Quote: ... and anti-GFP (Clontech 632381 1:2000). The membrane was then washed three times in TBST and incubated with goat-anti-rabbit (Jackson ImmunoResearch 111-035-046 1:5000 ...
• APP binds to the EGFR ligands HB-EGF and EGF, acting synergistically with EGF to promote ERK signaling and neuritogenesis

  Joana F. da Rocha, et al., bioRxiv - Neuroscience 2020

  Quote: ... expression vector pAS2-1 (Clontech, Enzifarma, Portugal). Bait-1 cDNA ...