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Citations for Takara Bio :
601 - 650 of 1441 citations for Son of sevenless homolog 1 SOS1 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... NPR1-GFP proteins were detected by reacting the blots with the mouse monoclonal anti-GFP monoclonal antibody (Clontech, USA) and horseradish peroxidase-conjugated secondary antibody (Santa Cruz ...
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bioRxiv - Immunology 2021Quote: ... Antibodies were produced by transient transfection of human epithelium kidney 293T Lenti-X cells (Clontech Laboratories, Mountain View, CA) using the JetPrime transfection kit (Polyplus Transfection Illkirch ...
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bioRxiv - Microbiology 2023Quote: ... and subsequently stained using a horseradish peroxidase (HRP)-conjugated monoclonal anti-StrepTag antibody (Iba) or mouse anti-6His (Clontech) monoclonal antibody combined with rabbit anti-mouse-HRP polyclonal antibodies (DAKO) ...
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bioRxiv - Neuroscience 2023Quote: ... The next primary antibodies were used at the specified concentrations: rabbit anti-Dsred Pab (Takara Bio Cat# 632496, RRID:AB_10013483) [1:500] ...
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bioRxiv - Biochemistry 2023Quote: ... The membrane was incubated for 1 h at room temperature with Odyssey blocking buffer and 0.2 % PBS-T (PBS containing 0.2 % (v/v) Tween-20) with rat anti-HA clone 3F primary antibody (Clontech) at 1:1,000 dilution ...
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bioRxiv - Plant Biology 2021Quote: ... qRT-PCR was performed on 1 μL of 1 in 40 diluted cDNA in 15 μL reactions using SYBR Premix Ex-Taq II reagent (Takara Bio) in a Bio-Rad CFX96 real-time system (see Table S1 for primer pairs) ...
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bioRxiv - Genetics 2020Quote: ... Membranes were blocked in 5% milk for 1 h at room temperature and then probed with mouse-anti-EGFP (for pYFP constructs; 1:8000; Clontech, 632380) or mouse-anti-V5 tag (1:2000 ...
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bioRxiv - Molecular Biology 2024Quote: FokI assays were conducted using reporter cell lines as described previously.22 Site-specific DSBs were induced by adding 1 µM Shield-1 (Takara, 632189) and 1 µM 4-hydroxytamoxifen (Sigma ...
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bioRxiv - Systems Biology 2022Quote: ... The blocked membrane was incubated overnight at 4 ℃ in primary antibodies: mouse polyclonal anti-Cas9 (Takara Bio, cat. no. 632607), mouse monoclonal anti-β-actin (8H10D10 ...
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bioRxiv - Cell Biology 2022Quote: ... The transformants were screened by microscopically observing the GFP signals and further confirmed by a western blotting analysis with a polyclonal anti-GFP antibody from Clontech. In addition ...
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bioRxiv - Cell Biology 2022Quote: ... and the presence of the kinA-GFP fusion was confirmed by western blotting analysis with a polyclonal anti-GFP antibody from Clontech. In addition ...
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bioRxiv - Cancer Biology 2020Quote: ... the TET protein expression in each clone was checked by immunoblotting using TetR monoclonal antibody (Clone 9G9) (Clontech, cat#631131). In addition ...
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LptM promotes oxidative maturation of the lipopolysaccharide translocon by substrate binding mimicrybioRxiv - Microbiology 2023Quote: ... membranes were incubated with epitope-specific rabbit polyclonal antisera or with an anti poly-histidine horseradish peroxidase-conjugated monoclonal antibody (TaKaRa). Immunodetection was revealed by using a Clarity Western ECL blotting substrate (BioRad ...
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bioRxiv - Plant Biology 2023Quote: ... The tissue was fixed for 30 min and the immunoprecipitation performed using a SEP3-specific antibody followed by library preparation using ThruPLEX DNA-Seq Kit (Takara) and deep sequencing65 ...
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bioRxiv - Biophysics 2022Quote: ... The stability of the fusion constructs was verified by gel electrophoresis and immunoblotting using an anti-GFP primary antibody (JL-8 monoclonal, Takara). Point mutations were introduced by site-directed mutagenesis (New England Biosciences) ...
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bioRxiv - Plant Biology 2024Quote: ... S1 and P1 fractions were loaded in denaturing buffer for SDS-PAGE separation and immunoblotted with the indicated antibodies (anti-DDB2: Molinier et al., 2008; anti-GFP: Takara-632593 ...
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bioRxiv - Microbiology 2023Quote: ... Blots were probed for 1h at room temperature (RT) with a mouse anti-GFP antibody (Living Colors -JL-8, BD Biosciences Clontech) in TBS-T buffer (20 mM Tris ...
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bioRxiv - Plant Biology 2023Quote: ... transferred membranes were hybridized overnight at 4 °C with the following primary antibodies: anti-GFP (Takara Bio Cat# 632381, RRID:AB_2313808) (1/5000) ...
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bioRxiv - Cell Biology 2024Quote: ... and the presence of ΔTM-VezA-GFP was confirmed by western blotting analysis with a mouse monoclonal anti-GFP antibody from Clontech. In addition ...
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bioRxiv - Bioengineering 2024Quote: ... The rituximab and cetuximab antibody variable sequences were generated by IDT and cloned into the variable regions of the VRC01 antibody plasmid vector (a generous gift from the Kim lab at Stanford) by using the In-Fusion cloning kit (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... followed by SDS–PAGE and immunoblotting with goat anti-GST (1:3000, Cytiva, 27-4577-01) and mouse anti-6xHis (1:3000, Takara Bio, 631212) antibodies ...
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bioRxiv - Physiology 2022Quote: ... Cleared lysates were loaded into wells of the SDS-agarose gels (1% SeaKem Gold Agarose [Lonza, Basel, Switzerland], 30% glycerol, and 1 × Tris/glycine/SDS [TG-SDS] buffer [Takara Bio Inc.]) and then electrophoresed in a running buffer (1 × TG-SDS buffer and 10 mM 2-mercaptoethanol ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit α-RFP (1:500; Clontech, cat# 632496, RRID:AB_10013483); mouse α-lacZ antibody (1:100 ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were induced with 1 µM rapalog (AP21967, Takara) for 45 minutes at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... 10 U ml−1 of HRV- 3C protease (Takara) were added to the Ni-NTA eluted protein to remove the oligohistidine- GST-tag during dialysis against 3 L of dialysis buffer I (25 mM Tris-HCl ...
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bioRxiv - Genomics 2020Quote: ... was cloned into modified pCold-1 (Takara Bio Inc.), in which the factor Xa cleavage site was replaced with tobacco etch virus (TEV ...
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bioRxiv - Neuroscience 2021Quote: 1 part of Lenti-X™ concentrator (TaKaRa; 631232) was first mixed with 3 parts of supernatant and incubated at 4 °C overnight for lentivirus precipitation ...
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bioRxiv - Microbiology 2020Quote: ... Ab anti-GFP (Clontech #632592, WB dilution 1:1000), Ab Beta Actin HRP conjugated (Abcam ...
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bioRxiv - Developmental Biology 2021Quote: ... rabbit poly-clonal anti-GFP (1/800, 632592, Clontech), anti-Myosin heavy chain (1/10 ...
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bioRxiv - Neuroscience 2020Quote: ... and dsRed (1:600, Takara Bio, 632496; RRID: AB_10013483) was performed on adult brain sections of quadruple transgenic zebrafish ...
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bioRxiv - Neuroscience 2020Quote: ... anti-human nuclei (STEM101; Takara, Y40400, IgG1, 1:100) and anti-human NOGOA (Santa Cruz Biotechnology ...
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bioRxiv - Neuroscience 2021Quote: ... incubated with α-MYC (mouse, 1:500, Clontech #631206) in 4 % BSA-PBS for 60 min at room temperature to stain surface expressed GluK2 ...
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bioRxiv - Neuroscience 2021Quote: - 1/1000 Rabbit α-dsRed (Takara, Living Colors 632496)
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bioRxiv - Biochemistry 2020Quote: ... according to the Yeast Protocols Handbook (PT3024-1, Clontech).
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bioRxiv - Cell Biology 2021Quote: ... rabbit anti-dsRed dilution 1:100 (IF) (Clontech, 632496); mouse anti-M6PR dilution 1:100 (IF ...
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bioRxiv - Physiology 2021Quote: ... 1 mL of Fruit-mate (Takara, catalog no.: 9192) was added ...
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bioRxiv - Cell Biology 2022Quote: ... monoclonal anti-GFP JL-8 (1:1000, Clontech 632380), polyclonal rabbit anti-RP2 (1:1000 ...
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bioRxiv - Microbiology 2022Quote: ... we used 1 μL of Extaq enzyme from Takara on genomic DNA (20 μg ...
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bioRxiv - Neuroscience 2021Quote: ... and 0.4 U µl−1 recombinant RNase inhibitor (Clontech)) using a Wheaton Dounce tissue grinder (15 strokes with the loose pestle) ...
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bioRxiv - Genomics 2021Quote: ... 1 μL of T4 DNA ligase (Takara Bio Inc.) was added ...
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bioRxiv - Cell Biology 2022Quote: ... and mouse monoclonal anti-GFP (Clontech; milk; 1:2,000).
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bioRxiv - Neuroscience 2021Quote: ... and mouse anti-DSRed (targeting mCherry; 1:2000; Clontech), washed ...
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bioRxiv - Microbiology 2021Quote: ... and 10 μL (1 mU) of Pfu PGAP (TaKaRa) was added ...
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bioRxiv - Neuroscience 2021Quote: ... 1× Universal Primer Mix (first PCR, Clontech Laboratories, Inc.) or 1× Nested Universal Primer (secondary PCR ...
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bioRxiv - Microbiology 2021Quote: ... 1 μL of PrimeScript One Step Enzyme Mix (TaKaRa), 10 μL of 2× One Step RT-PCR buffer (TaKaRa) ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit-anti-RFP (1:500, Clontech 600-401-379); mouse-anti-24B10 (Zipursky et al. ...
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bioRxiv - Microbiology 2020Quote: ... and 10 U μl−1 PrimeScript Reverse Transcriptase (Takara) at 48 °C for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... and mCherry (anti-dsRed polyclonal, 1:1000; Clontech, 632496). Sections were then washed in phosphate buffered saline (PBS ...
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bioRxiv - Cell Biology 2023Quote: ... anti GFP (mouse monoclonal, JL8, Clontech; WB: 1:6000), anti-human tubulin (mouse monoclonal ...
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bioRxiv - Cancer Biology 2023Quote: ... monosodium salt (WST-1) (Takara BioInc, Clontech Laboratories, Inc.). 4 × 103 cells/ml was cultured in 5 replicates in 96-well plates ...