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Citations for Takara Bio :
601 - 650 of 1988 citations for Recombinant Zebrafish IL 1 beta Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... The solubilized protein was batch bound to Co-NTA resin (Takara Bio USA), washed with buffer A with 10 mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... monoclonal antibody for green fluorescent protein (JL-8; Takara Bio, Kusatsu, Japan; recognizes VC), rabbit anti-GFP (D5.1 Cell Signaling Technology 2956S ...
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bioRxiv - Plant Biology 2022Quote: ... CITRINE-fusion proteins were detected with anti-GFP antibody (JL-8, Takara Bio Clontech, dilution ...
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bioRxiv - Microbiology 2021Quote: ... The protein was purified from the supernatant by TALON metal affinity resin (Clontech, #635606) and eluted with 150 mM imidazole ...
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bioRxiv - Microbiology 2021Quote: ... The protein concentration of each tissue was determined by BCA method (TaKaRa, Lot#A4401A). The tissue protein was used for SDS-PAGE electrophoresis at the same quality ...
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bioRxiv - Cell Biology 2020Quote: ... target proteins were purified from cell lysate by Ni-iminodiacetic acid affinity chromatography (Clontech). However ...
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bioRxiv - Plant Biology 2021Quote: ... GFP-tagged proteins were detected using the anti-GFP antibody (JL-8, Clontech Takara) diluted 1:2000 (v/v ...
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bioRxiv - Plant Biology 2021Quote: ... GFP-tagged proteins were detected using the anti-GFP antibody (JL-8, Clontech Takara) diluted 1:2000 (v/v ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were first purified by affinity chromatography using TALON metal affinity resin (TaKaRa), and further purified via gel filtration chromatography on a Superdex 200 column (GE Healthcare ...
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bioRxiv - Genomics 2022Quote: Whole bladder protein lysate was obtained by homogenizing bladder tissue using Biomasher II (TaKaRa) in ice-cold RIPA buffer with the protease inhibitor (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... GFP fusion proteins were revealed with mouse monoclonal antibody anti-GFP (JL-8, Clontech) used at 1/2000 dilution ...
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bioRxiv - Molecular Biology 2024Quote: ... The expressed protein was then purified using TALON Metal Affinity Resin (Takara Cat. # 635502) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentration was determined by bicinchoninic acid assay using bovine serum albumin (TaKaRa Bio) as the reference standard ...
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bioRxiv - Cell Biology 2022Quote: Protein extracts were analyzed by SDS-PAGE and immunoblotting using α-GFP (monoclonal, Takara), α-mCherry (polyclonal ...
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bioRxiv - Developmental Biology 2023Quote: ... The concentration of tissue extracts was quantified using a BCA Protein Assay Kit (Takara). Samples were electrophoresed on 10% TGX Stain-Free gel (Bio-Rad ...
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bioRxiv - Biophysics 2024Quote: ... Soluble protein in the supernatant was purified by Talon metal affinity resin (Takara Bio) and eluted into the elution buffer (50 mM sodium phosphate buffer (pH 7.2 ...
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bioRxiv - Plant Biology 2024Quote: ... Rwt4Ca and its variant proteins were cloned into the prey vector pGADT7 (Takara, #630442) while AvrPwt4 and VirPwt4 were cloned into the bait vector pGBKT7 (Takara ...
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bioRxiv - Cell Biology 2024Quote: Cells expressing inducible fluorescent proteins were induced with 100 ng/ml doxycycline (Clontech #631311) for 24 h ...
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bioRxiv - Cancer Biology 2024Quote: ... The expressed proteins were purified by metal affinity chromatography (IMAC) using TALON resin (Takara) as manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... 1 μgml−1 doxcycline (TAKARA Bio) and 10−6 M dexamethasone (Sigma Aldrich).
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bioRxiv - Molecular Biology 2023Quote: ... 1 µM Shield-1 (Takara # 632189) for 4 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... After purification of the His6-SUMO-binder fusion proteins using TALON metal affinity resin (Clontech), they were digested with SUMO protease 1 ...
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bioRxiv - Biophysics 2021Quote: ... Protein constructs were expressed under control of a CMV promoter using pEGFP-C1/N1 (Clontech) (enhanced GFP ...
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bioRxiv - Cancer Biology 2021Quote: ... plasmid or the enhanced green fluorescent protein (EGFP) plasmid (pEGFP-C1, Clontech/Takara Bio Europe). Transfections were achieved using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... plasmid or the enhanced green fluorescent protein (EGFP) plasmid (pEGFP-C1, Clontech/Takara Bio Europe). Transfections were achieved using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Physiology 2020Quote: ... transfections also contained 0.2 μg of the pEGFP plasmid (Enhanced Green Fluorescent Protein plasmid, Clontech), and EGFP expression served as a marker of transfection ...
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bioRxiv - Biophysics 2021Quote: ... The solubilized protein was incubated with 2 mL TALON cobalt metal-affinity resin (Takara Bio) for 1 h ...
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bioRxiv - Microbiology 2020Quote: ... to remove cellular debris and the protein was column-purified using anti-histidine resin (ClonTech). The resin was washed ten times with 10x column volumes of wash buffer mixed with an increasing proportion of tris buffer (20 mM Tris-HCl ...
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bioRxiv - Biophysics 2021Quote: ... the protein was affinity purified using TALON cobalt affinity resin (Clontech Laboratories, Mountain View, CA) followed by size exclusion chromatography on Superdex 200 10/30 GL size exclusion column (GE Healthcare ...
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bioRxiv - Molecular Biology 2021Quote: ... and the protein concentration was measured by a bicinchoninic acid (BCA) assay kit (Takara Bio) using bovine serum albumin as the standard ...
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Role of the Topoisomerase IIα Chromatin Tether domain in Nucleosome Binding & Chromosome SegregationbioRxiv - Cell Biology 2021Quote: ... His6-tagged protein in the supernatant was captured on Talon Sepharose beads (#635502, Clontech/Takara) and pre-equilibrated against Buffer1 (300 mM NaCl ...
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Role of the Topoisomerase IIα Chromatin Tether domain in Nucleosome Binding & Chromosome SegregationbioRxiv - Cell Biology 2021Quote: ... His6-tagged protein in the supernatant was captured on Talon Sepharose beads (#635502, Clontech/Takara) and pre-equilibrated against Buffer1 (300 mM NaCl ...
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bioRxiv - Evolutionary Biology 2022Quote: CDS sequence coding protein BmMETTL3(204-329) was cloned into the Pcold vector (TaKaRa, China). Primers were listed in Table S1 ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins were purified by immobilized-metal affinity chromatography using TALON® metal affinity resin (Clontech), where filtered supernatant (0.45 μm filter ...
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bioRxiv - Neuroscience 2022Quote: 50 µg of human cerebral cortex lysate (Protein Medley, Takara, Kusatsu, Japan, Cat. No. 635323) were diluted and prepared according to manufacturer’s instructions (protocol PT1602-1) ...
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bioRxiv - Molecular Biology 2022Quote: The respective sequences of the proteins were cloned into pGBKT7 and pGADT7 vectors from Clontech which harbor either the DNA binding domain or activation domain at the N-termini ...
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bioRxiv - Biochemistry 2023Quote: ... The secreted dystroglycan protein DAG128–749R311A/R312A was purified using His60 Ni IMAC resin (Takara) and subjected to anion exchange using DEAE resin ...
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bioRxiv - Biophysics 2024Quote: ... The protein was purified to homogeneity using a combination of TALON Metal Affinity Resin (Takara) and gel filtration chromatography ...
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bioRxiv - Microbiology 2024Quote: ... The fusion protein was affinity-purified using TALON Metal Affinity Resin (Takara Biosciences; Table S4) and eluted in buffer containing 25 mM HEPES ...
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bioRxiv - Biochemistry 2024Quote: The Upf1-HD-His6 wild-type protein was first purified on a TALONTM resin (Clontech) equilibrated with buffer A200 ...
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bioRxiv - Plant Biology 2023Quote: ... Protein concentration was measured using a BCA assay kit (Takara Bio Inc., Kusatsu, Siga, Japan). Twenty micrograms (µg ...
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bioRxiv - Biophysics 2023Quote: ... the protein was affinity purified using TALON cobalt affinity resin (Clontech Laboratories, Mountain View, CA) followed by size exclusion chromatography on Superdex 200 10/30 GL size exclusion column (GE Healthcare ...
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bioRxiv - Bioengineering 2023Quote: ... Proteins were purified from the soluble cell lysate using His60 Ni Superflow Resin (Takara Bio) and concentration was measured using NanoDrop ...
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bioRxiv - Biochemistry 2024Quote: The various proteins were produced in Escherichia coli strain BL21(DE3)-pGro7/GroEL strain (TaKaRa). The sequences were enhanced by an N-terminal strep tag (WSHPQFEK ...
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bioRxiv - Plant Biology 2024Quote: ... Purification of the His6-tagged protein was achieved by TALON® spin columns (Takara Bio) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... The suspension was analyzed with a TaKaRa BCA Protein Assay Kit (T9300A, TaKaRa, Kusatsu, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... were mixed at a 1:1:1 ratio and bound to retronectin (Clontech)-coated plates according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... protein solution was collected and subjected to purification using His60 Ni Superflow resin (TaKaRa, California, USA) to remove 6×His-SUMO tag from the protein preparations ...
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bioRxiv - Microbiology 2022Quote: ... The supernatants containing soluble target proteins were loaded into a Talon metal affinity resin (Clontech, USA). After washing three times with buffer A ...
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bioRxiv - Molecular Biology 2023Quote: Protein interactions in vivo in yeast were assayed using the ‘Matchmaker Gold Two-hybrid System’ (Clontech) using the protocols provided by the supplier ...