2 1 1 Diethoxy 2 methyl propyl 4' Nitrophenyl Carbonate d6 citations for Takara Bio

Quote: Yeast strain AH109 was transformed with bait (pGBKT7) and prey (pGADT7) constructs by following the small scale yeast transformation protocol from Yeastmaker™ Yeast Transformation System 2 (Clontech). Upon transformation ...

Identification of protein-protected mRNA fragments and structured excised intron RNAs in human plasma by TGIRT-seq peak calling

Jun Yao, et al., bioRxiv - Genomics 2020

Quote: ... and the resulting DNase I-treated RNA (~2 ng) was processed for sequencing by using a SMART-Seq v4 Ultra Low Input RNA kit (Takara Bio) according to the manufacturer’s protocol with 12 cycles of PCR followed by two rounds of clean up with 1.3X Agencourt AMPure XP beads (Beckman Coulter) ...

Liberibacter solanacearum interacts with host psyllid vitellogenin with its membrane proteins

Poulami Sarkar, Murad Ghanim, bioRxiv - Molecular Biology 2021

Quote: ... The first stranded cDNA was next amplified to produce double stranded cDNA in 20 amplification cycles by long distance PCR using the Advantage 2 polymerase mix (Takara, Clontech) following the manufacturer’s instructions ...

A framework for in situ molecular characterization of coral holobionts using nanopore sequencing

Quentin Carradec, et al., bioRxiv - Genomics 2020

Quote: ... PCRs were performed on board using 25 ng of DNA from each coral sample with the Advantage 2 kit (Takara Clontech) and a final primer concentration of 0.5 μM in a final reaction volume of 50 μl ...

Profiling of N⁶-methyladenosine dynamics indicates regulation of oyster development by m⁶A-RNA epitranscriptomes

Lorane Le Franc, et al., bioRxiv - Genomics 2021

Quote: ... were used for library construction using the SMARTer Stranded Total RNA-Seq Kit v.2 (634418, Pico Input Mammalian, Takara/Clontech, Japan) according to the manufacturer’s protocol without RNA fragmentation ...

Analysis of Intermolt and Postmolt Transcriptomes Provides Insight into Molecular Mechanisms of the Red Swamp Crayfish, Procambarus Clarkii Molting

Shengyan Su, et al., bioRxiv - Genomics 2020

Quote: ... 2 μg of total RNA per sample was used to generate first-strand cDNA using reverse transcription system (Takara, Dalian, China). Quantitative RT-PCR was performed using SYBR Premix Ex Taq (Takara ...

A comparative analysis of memory B cell and antibody responses against Plasmodium falciparum merozoite surface protein 1 in children and adults from Uganda

S. Jake Gonzales, et al., bioRxiv - Immunology 2021

Quote: ... Single stranded cDNA was synthesized immediately by adding 0.7 µl SMARTScribe reverse transcriptase (100 U/µl, f/c 2 U/µl, Takara Bio #639537), 7 µl First-Strand buffer (f/c 1×) ...

Directed evolution of Anabaena variabilis phenylalanine ammonia-lyase (PAL) identifies mutants with enhanced activities

Zachary JS Mays, et al., bioRxiv - Bioengineering 2020

Quote: ... The lysate was loaded onto a prepared column with 2 mL TALON Metal Affinity Resin (Clontech Laboratories, Inc., Mountain View, CA). After being washed twice with 5 column volumes (CV ...

Critical role of the periplasm in copper homeostasis in Gram-negative bacteria

Jun-ichi Ishihara, et al., bioRxiv - Systems Biology 2020

Quote: ... proteins in whole cell extract and immunoprecipitated fractions were digested with 2 mg/mL proteinase K (Takara Bio Inc., Kusatsu, Japan) at 42°C for 2 h ...

CCR4 blockade leads to clinical activity and prolongs survival in a canine model of advanced prostate cancer

Shingo Maeda, et al., bioRxiv - Cancer Biology 2021

Quote: We quantified mRNA expression levels of IL-10 and chemokines identified in the RNA-Seq analysis using 2-step real-time RT-PCR (Thermal Cycler Dice Real Time System, Takara Bio). The ribosomal protein L13a (RPL13A ...

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