Labshake search
Citations for Takara Bio :
501 - 550 of 773 citations for Vertical Electrophoresis system since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... of PINK1 or OMA1 encoding plasmid using appropriate primers followed by Gibson assembly (In-Fusion HD Cloning system, Clontech) into the SalI-XhoI of the pLenti-CMV-Neo vector ...
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bioRxiv - Immunology 2022Quote: ... Recombinant adenovirus vaccines were produced using the Adeno-X™ Adenoviral System 3 according to the manufacturer’s manual (Takara Korea Biomedical Inc. ...
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bioRxiv - Plant Biology 2022Quote: ... A cDNA library constructed using switchgrass leaves’ cDNA were screened following the Matchmaker Gold Y2H system user manual (Clontech). The library screening was performed on SD/-Trp-Leu-Ade-His medium ...
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bioRxiv - Plant Biology 2022Quote: Yeast-two-hybrid library screening was performed according to the Matchmaker Gold Yeast Two-Hybrid System User Manual (Clontech).
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bioRxiv - Microbiology 2023Quote: ... and fluorescent signals from resulting PCR products were acquired using a Thermal Cycler Dice Real Time System III (Takara). Finally ...
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bioRxiv - Plant Biology 2023Quote: ... Yeast transformation was performed as described in the Yeastmaker Yeast Transformation System 2 User Manual (Clontech, TaKaRa Bio, USA). Serial dilution of transformed colonies were dropped on –Trp/-His synthetic dropout selection medium ...
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bioRxiv - Plant Biology 2023Quote: ... Yeast transformation was performed as described in the Yeastmaker Yeast Transformation System 2 User Manual (Clontech, TaKaRa Bio, USA). Serial dilution of transformed colonies were dropped on –Trp/-His synthetic dropout selection medium ...
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bioRxiv - Microbiology 2023Quote: ... High-throughput qPCR (HT-qPCR) was performed by the Smart Chip Real-time PCR system (Takara Bio USA, Inc.). A non-template negative control was set for each primer set ...
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bioRxiv - Genetics 2023Quote: ... The transformation process followed the protocol described in the Yeastmaker™ Yeast Transformation System 2 User Manual (Takara Bio), yielding approximately 2 × 106 and 7 × 106 transformants for LE9 and BLX strains ...
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bioRxiv - Molecular Biology 2023Quote: The Yeast Two-Hybrid assays were performed according to the Matchmaker Gold Yeast Two-Hybrid System (Cat#630489; Clontech) kit instructions ...
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bioRxiv - Bioengineering 2023Quote: ... cerevisiae was carried out by using the lithium acetate method of Yeastmaker™ Yeast Tansformation System (CLONTECH Laboratories, Inc.). The plasmid of pICASA was cleaved by XbaI and integrated into the chromosomal DNA of S ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative RT-PCR was performed using a Thermal Cycler Dice Real Time System II (Takara Bio Inc., Otsu, Japan) and SYBR Premix Ex Taq II (Takara ...
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bioRxiv - Evolutionary Biology 2023Quote: ... coli BL21 (DE3) containing a pGro7 plasmid for overexpression of the GroEL/ES chaperone system (Takara Bio, Shiga, Japan). ACP (acyl phosphatase ...
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bioRxiv - Evolutionary Biology 2024Quote: ... All yeast 2-hybrid screens were done using the Matchmaker® Gold Yeast Two-Hybrid System (Takara Bio USA) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... The Y2H assay was carried out based with the manufacturer’s protocol of Matchmaker Gold Yeast Two-Hybrid System Kit (Takara). The primers used for vector construction are listed in Supplemental Data Set S1.
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bioRxiv - Microbiology 2024Quote: Quantitative PCR (qPCR) was conducted using a Thermal Cycler Dice Real Time System III (Takara Bio Inc., Shiga, Japan). For each 25 μL reaction ...
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bioRxiv - Neuroscience 2024Quote: ... Real-time PCR was performed using a Thermal Cycler Dice Real-Time System II TP900 (Takara Bio, Shiga, Japan), and the relative copy number of various gene transcripts per beta-actin transcript was determined using calibration standards for each tested molecule ...
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bioRxiv - Bioengineering 2024Quote: ... [50] The ssDNA scaffold was initially prepared using Guide-it Long ssDNA Production System v2 kit (Takara Bio, 632666) following manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... or a custom cDNA library made from entire mature leaf material (Make Your Own “Mate & Plate” Library System, Clontech). Trimeric interactions (Y3H ...
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bioRxiv - Plant Biology 2020Quote: ... and Real-time qRT-PCR was performed using a Thermal Cycler Dice® Real Time System III T950 (Takara, Japan) with gene-specific PCR primers (Supplemental Table S1) ...
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bioRxiv - Plant Biology 2020Quote: ... The cDNA library was ligated to pGADT7-Rec vector to prepare recombinant AD construct using Make Your Own “Mate and Plate” Library system (Clontech). The yeast strain Y2H gold was co-transformed with bait and prey recombinant construct and colonies were screened against DDO (SD/-Leu/-Trp ...
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bioRxiv - Neuroscience 2021Quote: ChIP-seq libraries were generated from ChIP-DNA using a custom Illumina library type on an automated system (Apollo 342, Wafergen Biosystems/Takara). ChIP-seq libraries were sequenced on Illumina NextSeq 500 as single-end 75bp reads (Active Motif).
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bioRxiv - Microbiology 2021Quote: ... pBICP35-CE1–CE8 transient expression vectors under the control of the 35S promoter were constructed using an In-Fusion system (Clontech, TaKaRa). The fragment containing the cDNA of CE1 was amplified with the primers 35S_CE1_Fw and 35S_CE1_Rv ...
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bioRxiv - Molecular Biology 2021Quote: The Tet-on vector was obtained from the Lenti-X Tet-One Inducible Expression System (Puro) (Clontech, Cat. No. 634847). We transfected 1 × 106 HDR-immortalized cells using the Neon nucleofection system and 10 μg of plasmid DNA (plasmid encoding Cre recombinase ...
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bioRxiv - Developmental Biology 2021Quote: ... The guide RNA was designed to target an immediate downstream of the stop codon of the human GATA3 gene and generated using the Guide-it sgRNA In Vitro Transcription System (Clontech). The target sequences of gRNAs are shown in Table S2 ...
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bioRxiv - Cell Biology 2020Quote: ... and first-strand cDNA was synthesized with 1 μg of total RNA using a SuperScript III First-Strand Synthesis System for RT-PCR (TaKaRa) following the manufacturer’s protocol.
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bioRxiv - Developmental Biology 2020Quote: ... RT-qPCR analyses were carried out with KAPA SYBR FAST qPCR kits using a Thermal Cycler Dice Real Time System (TaKaRa) according to the manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2020Quote: Doxycycline-inducible overexpression of HA-tagged proteins was achieved using the Lenti-X Tet-On 3G Inducible Expression System (Clontech) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... The resulting plasmids were co-transformed into Saccharomyces cerevisiae strain AH109 according to the protocols in the Matchmaker GAL4 Two-Hybrid System 3 (Clontech). The yeast cells were cultured on SD/-Leu-Trp (-LW ...
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bioRxiv - Molecular Biology 2021Quote: Stable 3T3 cells (ATCC) overexpressing zDHHC3 or green fluorescent protein (GFP) as a control were generated using the pLVX lentiviral system (Clontech). Cells were labeled by stable isotope labeling with amino acids in cell culture (SILAC ...
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bioRxiv - Cancer Biology 2019Quote: ... NEK9 lentiviral particles for each construct were harvested from transfected 293T packaging cells using the Lenti-X(tm) HTX Packaging System (Clontech), transduced into U2OS parental cells and selected in fresh media supplemented with 2 mg/ml of G418 and 1 µg/ml puromycin over several days ...
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A Plant-Specific Polarity Module Establishes Cell Fate Asymmetry in the Arabidopsis Stomatal LineagebioRxiv - Plant Biology 2019Quote: ... Directed yeast two-hybrid constructs were generated by ligation of cDNAs into vector components of the Matchmaker II system (Clontech). Bait and prey clones were cotransformed into yeast strain AH109 ...
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bioRxiv - Cancer Biology 2019Quote: ... Amplification and detection of mRNA were conducted by using the Thermal Cycler Dice® Real Time System (Takara Bio, TP800), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... Real-time PCR was carried out in triplicate with primers by using SYBR Green Realtime PCR Master Mix-Plus (Toyobo) in the Thermal Cycler Dice Real-Time PCR system (TaKaRa). PCR primers are listed in Supplemental Tables S2 and S3.
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bioRxiv - Molecular Biology 2019Quote: ... interaction test and plasmid isolation were performed using the Yeast Protocols Handbook and Matchmaker GAL4TM Two-hybrid System 3 & Libraries User Manual (Clontech).
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bioRxiv - Plant Biology 2021Quote: ... using primers and DNA templates listed in Table S4 were cloned into pLRE::LRE-cYFP plasmid linearized with SpeI-HF and AscI by using the In-Fusion HD Cloning Plus system (Clontech). Transformants in Col-0 background (pLRE::GUS ...
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bioRxiv - Cell Biology 2021Quote: Interactions between CDK-2 and COSA-1 were assayed using the Matchmaker Gold Yeast Two-Hybrid System (Clontech PT4084-1). CDK-2 and COSA-1 cDNAs were amplified from a C ...
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bioRxiv - Plant Biology 2021Quote: Yeast two-hybrid assays were performed as described (Cesari et al., 2013) using the Matchmaker Gold Yeast Two-hybrid system (Clontech).
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bioRxiv - Neuroscience 2020Quote: ... the 11kb genomic region encompassing kdm5 was amplified by PCR from w1118 and cloned into the pattB vector (Bischof et al., 2007) using the In-Fusion cloning system (Takara). A 3xHA tag was included in-frame at the 3’end of the kdm5 ORF ...
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bioRxiv - Microbiology 2021Quote: ... 214 and 321 were inserted between the XhoI and XbaI restriction sites of the pLew100-eGFPX plasmid using the In-Fusion cloning system (Clontech). The PEPCK gene was also in situ tagged at the N-terminal as described above.
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bioRxiv - Molecular Biology 2021Quote: ... The Y1H experiment was carried out according to the manufacturer’s protocol for the Matchmaker Gold Y1H library screening system (Clontech, USA). The primers are listed in Table S1.
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bioRxiv - Plant Biology 2020Quote: ... was then used for RT-qPCR using the primers shown in Supplementary Table S1 with THUNDERBIRD SYBR qPCR Mix (TOYOBO) on a Thermal Cycler Dice Real Time System (TaKaRa). Pcal KB211 oprE and recA were used to normalize gene expression ...
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bioRxiv - Microbiology 2020Quote: ... was co-transformed with different bait-prey combinations as indicated in Extended Data Fig.1 in accordance with instructions for Matchmaker GAL4 Two-Hybrid System 3 (Clontech). The transformed yeast was then screened in 4-dropout plates for the protein-protein interaction.
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bioRxiv - Microbiology 2020Quote: ... Transformation of AH109 cells with pGAD424- and pGBT9-based constructs by the lithium acetate method was performed following the guidelines in the Matchmaker two-hybrid system (Clontech). Double transformants were isolated on synthetic defined medium lacking leucine and tryptophan ...
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bioRxiv - Cancer Biology 2020Quote: ... CA-MSCs lentivirally transduced with a secreted luciferase construct were encapsulated in alginate and the media was sampled for secreted luciferase overtime using the Ready-to-glow Secreted luciferase reporter system (Takara).
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bioRxiv - Cancer Biology 2021Quote: ... The wild-type BTK construct was cloned in the base vector (Lenti-X Expression System Version EF1-a; Takara Bio). STable over-expression of BTK in ibrutinib-resistant DLBCL cells was performed by using lentiviruses expressing human wild-type BTK as described previously.[15]
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bioRxiv - Microbiology 2022Quote: ... Each of these prey plasmids and previously constructed bait plasmids were co-transformed into Y2HGold yeast strain using Yeastmaker Yeast Transformation System 2 (TaKaRa). The transformation mix was spread on DDO/X and QDO/X/A agar plates and incubated at 30°C for 3-5 days ...
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bioRxiv - Microbiology 2022Quote: ... The purified ds cDNA and SmaI linearized pGADT7-Rec were co-transformed into Y187 yeast strain using Yeastmaker Yeast Transformation System 2 (TaKaRa). The transformation mix was spread on to the SD/-Leu agar plates and incubated at 30°C for 3–4 days ...
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bioRxiv - Genetics 2022Quote: ... The protein-coding genomic sequence including 3 kbp promoter sequence was PCR-amplified and cloned into the pGreen0029 binary vector (Hellens et al., 2000) using In-Fusion Cloning system (Takara Bio Europe ...
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bioRxiv - Cell Biology 2022Quote: ... reverse transcription and cDNA synthesis were performed using the SMART-Seq v4 Ultra Low RNA Kit for the Fluidigm C1 System (Takara) reagents ...