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Citations for Takara Bio :
501 - 550 of 878 citations for IFN γ Receptor II Human CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: The coding sequence of human PAC (NP_060722) previously subcloned into pIRES2-EGFP vector (Clontech) using XhoI and EcoRI restriction enzyme sites9 was used for whole-cell patch clamp recording ...
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bioRxiv - Immunology 2023Quote: Immunocult-stimulated human CD3+ T cells were retrovirally transduced on Retronectin-coated plates (TaKaRa). 500 µL of cells per well at 0.5 × 106 cells/mL were supplemented with 0.5 mL retroviral supernatant and centrifuged for 90 minutes at 900 g at 32°C ...
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bioRxiv - Neuroscience 2023Quote: ... GFP-tagged human tauKQ and tauP301L were expressed from the pEGFP-C1 plasmid (Clontech). Expression of mApple or GFP in cultured neurons was done using pGW1 mApple and pEGFP-C1 plasmids ...
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bioRxiv - Systems Biology 2023Quote: ... before library preparation using the SMARTer Human TCR α/β Profiling Kit (Takara Bio). In brief ...
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bioRxiv - Cancer Biology 2023Quote: Human DHRS7 was expressed as a GFP fusion using the pEGFP-N2 vector (Clontech) generated previously [5] ...
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bioRxiv - Synthetic Biology 2023Quote: Human Embryonic Kidney (HEK) 293 cells (ATCC, CRL-1573) and HEK293T-LentiX (Takara Biosciences) were cultured in standard culture conditions (37ºC and 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... Human CXCR4 was cloned into the pAcGFPm-N1 plasmid (Clontech Laboratories, Palo Alto, CA), as described (20).
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bioRxiv - Neuroscience 2024Quote: Human embryonic kidney cells (Lenti-X 293T Cell line from Takara. Cat no. 632180) were grown in DMEM/F12 media (GIBCO ...
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bioRxiv - Molecular Biology 2024Quote: The human embryonic kidney (HEK) 293T cell line was purchased from Takara (Cat# 632180) and cultured in a humidified 95% air / 5% CO2 incubator at 37°C in a standard Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Plant Biology 2020Quote: ... The qRT-PCR reaction mixture included TB Green® Premix Ex Taq™ II (Takara, Shiga, Japan). The qRT-PCR conditions were as follows ...
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bioRxiv - Immunology 2021Quote: ... using One Step TB Green(tm) PrimeScript(tm) RT-PCR Kit II (SYBR Green) (RR086B, TaKaRa, JAPAN). Relative copy number was determined by calculating the fold-change difference in the gene of interest relative to GAPTH ...
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bioRxiv - Plant Biology 2020Quote: ... and first-strand cDNA was synthesized using the PrimeScript II 1st strand cDNA Synthesis Kit (TAKARA Bio), according to the manufacturer’s protocol.
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bioRxiv - Genetics 2021Quote: ... and One Step TB Green™ PrimeScript™ RT-PCR Kit II (SYBR Green) (TaKaRa RR086B, Japan).
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bioRxiv - Plant Biology 2020Quote: ... and reverse-transcribed with a random hexamer using the PrimeScript II 1st strand cDNA Synthesis kit (TaKaRa). Gene specific primers were used to amplify cDNA by PCR using Ex Taq Hot Start Version (Takara) ...
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bioRxiv - Plant Biology 2020Quote: ... First-strand cDNA was synthesized using the PrimeScriptTM II 1st Strand cDNA Synthesis Kit (TAKARA, Kusatsu, Japan) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... qPCR or qRT-PCR was performed using TB Green® Premix Ex Taq™ II (Takara, Japan). 25S RNA and NbActin2 were used as internal references for DNA and RNA normalization ...
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bioRxiv - Microbiology 2020Quote: ... or (ii) the SMARTer Stranded Total RNA-Seq Kit v2 – Pico Input Mammalian libraries (Takara Bio USA) for ALG_1 and ALG_3 (Table S1) ...
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bioRxiv - Physiology 2021Quote: ... 2 µL of the synthesized cDNA was mixed with SYBR Premix Ex Taq II (Takara Bio Inc.) and 0.4 µM primers (same as above ...
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bioRxiv - Plant Biology 2021Quote: ... the RNA was converted into cDNA using PrimeScript™ II 1st Strand cDNA Synthesis Kit (TAKARA, 6210A) following the instruction in the manual ...
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bioRxiv - Plant Biology 2021Quote: ... Applied Biosystem StepOne real-time PCR system and SYBR Premix Ex Taq II kit (Takara, Dalian, China) were used for the qRT-PCR detection ...
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bioRxiv - Microbiology 2020Quote: ... The synthesized cDNA was subjected to RT-qPCR using TB Green Premix Ex Taq II (Takara Bio). The PCR conditions were set as follows ...
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The pan-cancer lncRNA PLANE regulates an alternative splicing program to promote cancer pathogenesisbioRxiv - Cancer Biology 2020Quote: ... containing 10 μl of TB Green Premix Ex Taq II (Tli RNaseH Plus) (TaKaRa, #RP820A; Dalian, China) and 0.4 μM of each primer ...
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bioRxiv - Plant Biology 2021Quote: ... Reverse transcription of RNAs were carried out using PrimeScript II 1st strand cDNA Kit™ (Takara, Japan). The coding sequences of DGAT1s were amplified by a high-fidelity KOD-Plus-Neo polymerase (Toyobo ...
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bioRxiv - Microbiology 2021Quote: ... and NEDD4 were evaluated using TB Green™ Premix Ex Taq™ II (Tli RNaseH Plus) (TAKARA). CFX96 Touch™ Real-Time PCR Detection System and CFX ManagerTM Software (Bio-Rad ...
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bioRxiv - Genomics 2019Quote: ... in a 20-μL reaction volume with 10 μL of 2× SYBR Premix Ex Taq II (TaKaRa), 2 μL of cDNA ...
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bioRxiv - Molecular Biology 2019Quote: ... qRT-PCR reactions were performed using a SYBR® Premix Ex Taq™ II Kit (TaKaRa, China) and a CFX96 real-time PCR detection system (BIO-RAD ...
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bioRxiv - Molecular Biology 2019Quote: ... Quantitative PCR was performed using a One Step TB Green PrimeScript RT–PCR Kit II (Takara, #RR086A) on a LightCycler 2.0 instrument (Roche) ...
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bioRxiv - Microbiology 2021Quote: ... and MSN4 were measured by RT-PCR using One Step SYBR PrimeScript RT–PCR Kit II (TaKaRa). Reactions were run on Mx3005P qPCR System (Agilent Technologies ...
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bioRxiv - Developmental Biology 2020Quote: ... DNase treated RNA was used for cDNA synthesis through PrimeScript II 1st Strand cDNA Synthesis Kit (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... and TB Green Premix Ex Taq II (Tli RNase H Plus) (Cat#: RR820A, TaKaRa Bio, Shiga, Japan). qPCR results were analyzed using LightCycler® 480 Software Version 1.5 (Roche Molecular Systems ...
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bioRxiv - Microbiology 2020Quote: cDNAs were synthesized by reverse transcription using a PrimeScript II 1st Strand cDNA Synthesis Kit (Takara Bio). Total RNA (1 μg ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was synthesized from 1 μg of total RNA with Prime Script II (Takara Bio, Daian, China). Quantitative real-time PCR analysis was performed using an ABI PRISM 7000 cycler (Applied Biosystems ...
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bioRxiv - Immunology 2021Quote: ... Real-time PCR was performed using SYBR® Premix Ex Taq™ II (Tli RNaseH Plus) (Takara) and complementary DNA was synthesized with a PrimeScript™ RT reagent Kit with gDNA Eraser (Takara) ...
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bioRxiv - Genetics 2022Quote: ... Reverse transcription for cDNA cloning was performed with PrimeScript™ II 1st Strand cDNA Synthesis kit (Takara), Gibson assembly was performed with NEBuilder HiFi DNA Assembly Master Mix ...
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bioRxiv - Physiology 2022Quote: ... Relative quantitative PCR was conducted with a TB Green Primer Ex Taq II (Takara Bio Inc., Japan) using LightCycler® 96 Real-Time PCR System (Roche ...
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bioRxiv - Synthetic Biology 2022Quote: ... The kit used for qPCR experiments was PrimeScript™ II 1st Strand cDNA Synthesis Kit (TaKaRa, Japan), HiScriptII 1st Strand cDNA Synthesis Kit (Vazyme Biotech Co. ...
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bioRxiv - Molecular Biology 2022Quote: ... The DNA band was cut out and purified using the Nucleospin® Extract II Kit (Clontech Laboratories) protocol ...
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bioRxiv - Immunology 2023Quote: Quantitative RT-PCR was performed with a SYBR Premix Ex Taq II (Tli RNaseH Plus) (RR820; Takara) on a 7500 Real-Time PCR system (Thermo Fisher) ...
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bioRxiv - Cell Biology 2023Quote: ... the first strand cDNA was prepared using PrimeScript II 1st Strand cDNA Synthesis Kit (Takara, Beijing, China). qPCR was performed using 2× SYBR Green qPCR Mix (Low ROX ...
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bioRxiv - Cancer Biology 2022Quote: ... Then real-time quantitative PCR was performed using TB Green® Premix Ex Taq™ II (Takara) on the Real-Time PCR Thermal Cycler qTOWER3 (Analytik Jena) ...
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bioRxiv - Developmental Biology 2023Quote: ... RT-qPCR was performed using a TB Green®Premix Ex Taq™II (TaKaRa, Lot: AL51019A), and β-actin gene was used as a reference gene ...
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bioRxiv - Neuroscience 2023Quote: ... Real-time quantitative PCR was performed with TB Green® Premix Ex Taq™ II (TAKARA, Japan) using a Bio-Rad CFX 96 real-time PCR detection system (Bio-Rad ...
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bioRxiv - Developmental Biology 2023Quote: ... RT-qPCR quantitation of mRNAs was performed using TB Green™ Premix Ex Taq™ II (TaKaRa) with Applied Biosystems StepOnePlus Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... Duke University Medical Center) and HeLa Tet-Off (HTO; human, female, cervix; Clontech, RRID: CVCL_V352) cells were cultured in high-glucose ...
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bioRxiv - Neuroscience 2021Quote: ... The WT hPNPO cDNA was amplified from the human brain cDNA library (TaKaRa, Cat #637242) [24] ...
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bioRxiv - Developmental Biology 2021Quote: ... cryosectioned brains were stained to detect expression of human cytoplasmic marker STEM121 (TaKaRa, cat. Y40410) and human-specific GFAP marker STEM123 (TaKaRa ...
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bioRxiv - Biochemistry 2020Quote: Human embryonic kidney 293T cells (‘293T’) and Lenti-X 293T cells were purchased from Clontech/Takara Bio (Mountain View ...
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bioRxiv - Cancer Biology 2021Quote: ... Virus transduction was performed using RetroNectin® Recombinant Human Fibronectin Fragment (Takara, cat.no. T100A/B) according to the manufacturer’s instructions (with centrifugation) ...
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bioRxiv - Neuroscience 2022Quote: ... Shortly, hiPSC lines (C1, C2, C3) and the commercial human iPSC-line Chipsc4 (Takara, Sweden) were cultured and differentiated into cortical NSCs as described elsewhere (23) ...
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bioRxiv - Cell Biology 2022Quote: Full length wild type human RORβ (NM_006914.3) was subcloned into the pLPCX retroviral vector (Clontech) using XhoI and NotI restriction enzymes (NEB ...