Labshake search
Citations for Takara Bio :
4951 - 5000 of 5597 citations for Hydrogen Peroxide Fluorescent Detection Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: 900 ng of total RNA isolated from HUVEC was used as input for SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Takara Bio). Sequencing was performed on the NextSeq500 instrument (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... for genotype analysis was extracted using the Qiagen DNeasy Blood and Tissue kit and PCR analysis was done with CloneAmp™ HiFi PCR Premix (Takara) or Phusion polymerase (NEB).
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bioRxiv - Neuroscience 2024Quote: ... RNA was extracted as described for RNA sequencing and cDNA was generated using the PrimeScript RT reagent kit (Takara, cat. #RR037A) using a mix of oligo dT and random hexamer primers ...
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bioRxiv - Cell Biology 2024Quote: ... Complementary DNA (cDNA) synthesis was carried out with 1 μg of RNA using the PrimeScript RT reagent kit (Takara-#RR037A-4). The cDNA was diluted and semiquantitative and Real-time PCR techniques were performed ...
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bioRxiv - Neuroscience 2023Quote: ... expressing UAS-eGFP was subjected to 14 cycles of PCR amplification (SMARTer Seq V4 Ultra Low Input RNA Kit; Takara Bio). 1 ng of amplified RNA was used to prepare cDNA libraries (Nextera XT DNA library preparation kit ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA samples were purified using a SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara, Mountain View, CA, USA), followed by library preparation using the Nextera XT DNA Library Prep kit (Illumina ...
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bioRxiv - Neuroscience 2023Quote: ... 5 μL of treated RNA was reverse transcribed using oligo d(T) primers (PrimeScript RT Reagent Kit, Takara Bio, Kyoto Japan). Then ...
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bioRxiv - Cell Biology 2023Quote: ... the purified DNA fragment of the MyoD gene was cloned into a pAAV-CMV vector using the In-Fusion Cloning HD Kit (Takara Bio). The DNA fragment corresponding to the 300 bp 5′ upstream region (−250 to +50 ...
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bioRxiv - Developmental Biology 2024Quote: ... genomic DNA removal and reverse transcription were carried out using the PrimeScript™ RT reagent kit with gDNA Eraser (Takara Bio).
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bioRxiv - Genomics 2024Quote: Illumina sequencing libraries are prepared by incorporating 1-10 ng of cfDNA with the ThruPLEX® Plasma-seq Kit by Takara Bio ...
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bioRxiv - Immunology 2023Quote: ... Reverse transcription was performed on 1 µg total mRNA per sample using PrimeScript™ RT Reagent Kit with gDNA Eraser (Takara) according to the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2024Quote: ... The Cys/Ser-substituted BPTI mutant (BPTI all-Cys/Ser) was constructed using the PrimeSTAR Mutagenesis Basal Kit (Takara Bio, Japan). BPTI all-Cys/Ser was overexpressed in Escherichia coli strain BL21(DE3 ...
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bioRxiv - Biophysics 2024Quote: ... PCR products were overlapped and assembled to create Vpr-StayGold with In-Fusion Snap Assembly cloning kits (Takara Bio, no. 638947); successful insertion was verified by sequencing.
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bioRxiv - Biophysics 2023Quote: ... 5’-CTGGAGAATCCCGGTGC-3’ and 5’- GTGTCAGATATATACATCCTGT-3’ and the PCR product was purified using a NucleoSpin Gel and PCR Clean-up Maxi kit (Takara Bio). The sequences of the 147 bp and 177 bp DNA are as follows:
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Accumulation of TCR signaling from self-antigens in naive CD8 T cells mitigates early responsivenessbioRxiv - Immunology 2023Quote: ... cDNA was prepared from 1000 cell equivalent of RNA using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara Bio), and next-generation sequencing libraries were generated using the Nextera XT DNA Library Preparation kit (Illumina) ...
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bioRxiv - Plant Biology 2023Quote: Plasmids for targeted gene deletion by homologous recombination were assembled using the In-Fusion® HD Cloning Kit (Takara Bio, Japan). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... Where possible equivalent amounts of total RNA (100pg) from all samples were used for first strand synthesis with the SmartERv3 kit (Takara Clontech) followed by 15-18 cycles of amplification (according to manufacturers’ instruction) ...
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bioRxiv - Microbiology 2022Quote: ... was used as the template for real-time RT-PCR performed according to the manufacturer’s protocol using One Step TB Green PrimeScript PLUS RT-PCR kit (Takara, Cat# RR096A) and the following primers ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... All PCR purifications were performed using the NucleoSpin® Gel and PCR Clean-up kit (TAKARA Bio Inc., San Jose, CA) according to manufacturer protocols ...
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bioRxiv - Biochemistry 2023Quote: ... Total RNA was further purified using mRNA Dynabeads and the purified mRNA was used as template for synthesis of cDNA using SMARTer PCR cDNA kit (Takara-Bio), with 18 cycles of amplification ...
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bioRxiv - Microbiology 2022Quote: ... RNA samples with sufficient material (10 pg–10 ng) were passed to whole-transcriptome library preparation using the SMART-Seq v4 PLUS Kit (Takara Bio) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The reverse transcription and the first round PCR were performed in a 25μl reaction volume by using PrimeScript™ One-Step RT-PCR Kit (TAKARA, RR055A). Cycling conditions were as follows ...
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bioRxiv - Systems Biology 2023Quote: ... 500 ng of isolated RNA was then used as input for generating cDNA and cDNA synthesis was performed using the PrimeScript RT kit (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... was used as the template for real-time RT-PCR performed according to the manufacturer’s protocol using One Step TB Green PrimeScript PLUS RT-PCR kit (Takara, Cat# RR096A) and the following primers ...
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bioRxiv - Microbiology 2023Quote: ... a complete cDNA sequence of bovine hnRNPM (NM_001191223) was obtained from genomic DNA of MDBK cells using PrimeScript RT reagent Kit (TaKaRa, Shiga, Japan), followed by a PCR reaction using PrimeSTAR Max DNA Polymerase (TaKaRa ...
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bioRxiv - Molecular Biology 2023Quote: ... All the mutant constructs of CE were prepared from pcDNA4/TO-bio-myc-mCE by using In-Fusion Site Directed Mutagenesis kit (Takara Bio). mCherry-H2B ...
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bioRxiv - Molecular Biology 2023Quote: ... A total of 1 μg of total RNA was reverse-transcribed with random primers using the PrimeScript™ 1st strand cDNA Synthesis Kit (TaKaRa) according to the manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2023Quote: ... iCaspase-T2A-tdTomato sequence was removed and swapped for mNeonGreen-NLS using a combination of Q5® High-Fidelity DNA Polymerase and the In-Fusion HD Cloning kit (Takara). Final targeting vector was generated by insertion of homology arms (∼600bp each ...
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bioRxiv - Developmental Biology 2023Quote: ... lentivirus was first packaged following protocols we described previously1 and was then concentrated 100x using Lenti-X Concentrator kit (Takara, # 631231). Concentrated lentivirus was aliquoted and stored in -80°C before use ...
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bioRxiv - Microbiology 2023Quote: ... TwistAmp® Basic Kit (TwistDx, TABAS03KIT), Milenia HybriDtect1 (TwistDX, Milenia01), UltraPure DNase/RNase-Free Distilled Water (Thermo, 10977015) and RNase inhibitor (Takara, 2313A). Thermo Fisher Nanodrop 1000 Spectrophotom was used to quantify nucleic acid ...
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bioRxiv - Developmental Biology 2023Quote: ... was eliminated by digesting with genomic DNA eraser buffer and cDNA was obtained by reverse transcription of RNA using PrimeScript RT reagent Kit with gDNA Eraser (Takara, RR047A). To measure the values of genes specifc primers ...
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bioRxiv - Immunology 2023Quote: ... total RNA (1 ng) was injected into the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech, Palo Alto, CA, USA) for sequencing ...
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bioRxiv - Cell Biology 2023Quote: ... An amount of 1 μg RNA was reverse transcribed into cDNA using the PrimeScript RT Reagent Kit (Takara Biomedical Technology, China). For RT-qPCR ...
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bioRxiv - Zoology 2023Quote: ... First- strand cDNA was synthesized from total RNA using the PrimeScript RT Reagent Kit with gDNA Eraser (Takara Bio, Shiga, Japan). PCR amplifications were performed using the KOD One PCR Master Mix (TOYOBO ...
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bioRxiv - Microbiology 2023Quote: ... was used as the template for real-time RT-PCR performed according to the manufacturer’s protocol using the OneStep TB Green PrimeScript PLUS RT-PCR kit (TAKARA, Cat# RR096A) and the following primers ...
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bioRxiv - Plant Biology 2023Quote: ... and inserted into the Aor51HI-linearized pMpGWB123/223/323/423 vectors using In-Fusion HD Cloning Kit (Takara Bio, Shiga, Japan).
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bioRxiv - Plant Biology 2023Quote: ... Each sample of total RNA (1 mg) was reverse transcribed by the PrimeScriptTM RT reagent Kit with gDNA Eraser (cat no. RR047A, Takara, Japan). The resistance genes examined were the same as those investigated in our previous study 9.
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bioRxiv - Developmental Biology 2023Quote: ... Reverse transcription and cDNA library preparation were performed with a SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech, 634888). cDNA was fragmented with a Covaris S 220 instrument (Covaris) ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA libraries were prepared using a SMARTer® Stranded Total RNA-Seq Pico Input Mammalian kit v2 (Takara Bio, Shiga, Japan), and sequencing was completed using a HiSeq 2500 System (Illumina ...
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bioRxiv - Developmental Biology 2023Quote: ... ATAC-identified enhancers were inserted into the ZED vector by In-Fusion cloning (Cat. #638910, In-Fusion HD Cloning Plus Kits, Takara Bio) using BspEI and BmgBI cutting sites for linearisation ...
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bioRxiv - Cell Biology 2023Quote: RACE-ready (Rapid Amplification of cDNA 5’ Ends) cDNA was generated using SMARTer PCR cDNA Synthesis Kit (#634925, Clontech Laboratories, Inc.) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2023Quote: ... was used as the template for real-time RT-PCR performed according to the manufacturer’s protocol using a One Step TB Green PrimeScript PLUS RT-PCR kit (Takara, Cat# RR096A) and the following primers ...
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bioRxiv - Plant Biology 2023Quote: ... were converted to cDNA with oligo dT primers according to the instructions of the PrimeScript RT reagent Kit with gDNA Eraser (Takara, Japan). Quantitative PCR was performed in accordance with manufacturer’s instructions for THUNDERBIRD SYBR qPCR Mix (TOYOBO ...
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bioRxiv - Genomics 2023Quote: ... purified via gel extraction from a 1% agarose gel followed by cleanup using the NucleoSpin PCR clean up and gel extraction kit (Takara Bio). Linearized CROP-seq-optiI and amplified oligonucleotides were assembled using the NEBuilder HiFi DNA assembly cloning kit (NEB ...
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bioRxiv - Cancer Biology 2022Quote: Sequencing libraries were prepared from RNA using the SMARTer Stranded Total RNA-Seq Kit v3 for picogram input material (Takara, #634488). Libraries were sequenced as paired-end 101 bp (tumoroid samples ...
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bioRxiv - Microbiology 2023Quote: ... RNA samples with sufficient material (10 pg–10 ng) were passed to whole-transcriptome library preparation using the SMART-Seq v4 PLUS Kit (Takara Bio) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... and the PCR products were subcloned into the pcDNA3.1(+) vector (digested with KpnI/BamHI) using an In-Fusion HD Cloning Kit (#Z9648N; TaKaRa, Kusatsu, Japan), resulting in pcDNA3.1(+ ...
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bioRxiv - Cell Biology 2022Quote: ... and the PCR product was subcloned into the pcDNA3.1(+) Ms Jaw1 PA (digested with XhoI) using an In-Fusion HD Cloning Kit (#Z9648N; TaKaRa, Kusatsu, Japan), resulting in pcDNA3.1(+ ...
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bioRxiv - Cell Biology 2022Quote: ... C-terminal PA vector was first prepared by insertion of a DNA cassette encoding the PA tag (annealed primer set 28) into the pcDNA3.1(+) vector (digested with XhoI/XbaI) using a DNA Ligation Kit (#6023; TaKaRa, Kusatsu, Japan). The DNA fragment coding for Ms Jaw1 was then amplified by PCR with primer set 29 from pTagGFP2-C Ms Jaw1 (previously described by Kozono et al. ...
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bioRxiv - Developmental Biology 2023Quote: ... Full length cDNA was generated using Clontech SMART-Seq v4 Ultra Low Input RNA kit for Sequencing (Takara Bio Europe, 634891) according to manufacturer’s instructions with 15 cycles of PCR for coding DMA (cDNA ...