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Citations for Takara Bio :
451 - 500 of 2391 citations for PCR Tube since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The amplification of viral DNA by PCR was carried out base on the manufacturer’s recommendations of the LA PCR Kit (TaKaRa Biomedical Technology Beijing, China). Briefly ...
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bioRxiv - Pathology 2022Quote: ... PCR was performed using Quick Taq HS DyeMix (Toyobo, Osaka, Japan) and a PCR Thermal Cycler Dice (TP650; Takara Bio Inc., Shiga, Japan), with the following cycling conditions ...
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bioRxiv - Microbiology 2020Quote: ... and qRT-PCR was performed on StepOne Plus Real-time PCR system (Applied Biosystem) with TB Green Premix Ex Taq II (Takara Cat no. RR820A). Briefly ...
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bioRxiv - Molecular Biology 2020Quote: ... Reverse transcription of the total RNA to complementary DNA and amplification of cDNA templates by long-distance PCR (LD-PCR) were performed using SMARTer Ultra-low Input RNA for Illumina Sequencing-HV (Clontech Labaratories, CA, USA) kit ...
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bioRxiv - Microbiology 2021Quote: RsmY and RsmZ expression quantification was done by RT-PCR using Mir-X miRNA qRT-PCR TB Green Kit (Takara Bio USA, Inc). For each reaction ...
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bioRxiv - Plant Biology 2019Quote: High-throughput qRT-PCR reactions were performed using the WaferGen SmartChip Real-time PCR system (5184-nanowells chip, WaferGen Bio-systems and Takara Bio Inc. USA). qRT-PCR reaction volumes of 100 nL consisted of 1 X LightCycler 480 SYBR Green I Master (Roche Diagnostics France) ...
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bioRxiv - Plant Biology 2020Quote: ... The band intensity of products obtained from semi-Q PCR was observed on the agarose gel and was further characterized with qRT-PCR using SYBR Green ® Premix (Takara Bio, USA). Specific primers were designed for studying expression of stress related genes in Arabidopsis and RP genes in rice using primer3 (v.0.4.0 ...
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bioRxiv - Microbiology 2023Quote: ... 5 μL of extracted RNA was subjected to one-step real-time RT-PCR using a One Step PrimeScript™ RT-PCR Kit (Perfect Real Time) (TaKaRa Bio Inc.) on a QuantStudio 5 Real-Time PCR system (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... The extracted RNAs were subjected to one-step real-time PCR using the One Step PrimeScript™ RT-PCR Kit (Perfect Real Time) (TaKaRa Bio Inc.).
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bioRxiv - Molecular Biology 2023Quote: ... was performed by quantitative real-time polymerase chain reaction (PCR) using the AAVpro™ Titration Kit (for Real Time PCR) Ver.2 (Takara Bio Inc), according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: Polymerase chain reaction (PCR) amplification of DNA receivers for plasmids construction and colony PCR were performed using PrimeSTAR® Max DNA Polymerase (R045B, TaKaRa, Kyoto, Japan). PCR primers were synthesized by Integrated DNA Technologies (IDT ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... 40 μL nematode liquid was transferred to a 1.5-mL Eppendorf tube with 40 μL of nematode lysis solution (Zhang et al., 2017) and 5 μL of protease K (Takara, Dalian, China). The mixture was then subjected to centrifugation at 2,000 ×g for 1 min before heating for 45 min at 65 °C in a constant temperature water bath ...
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bioRxiv - Neuroscience 2023Quote: ... Fifty cells were collected in a tube containing ice-cold lysis buffer and processed for cDNA synthesis according to the Smart-Seq v4 manual (Takara Bio, 634890) with a PCR cycle number of 11 ...
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bioRxiv - Immunology 2020Quote: ... cDNA was generated using a template□switch anchored reverse□transcription polymerase chain reaction (RT□PCR) based on the SMARTer pico cDNA PCR Synthesis Kit (Clontech, Mountain View, CA, USA). The final cDNA product was then subjected to a clean□up step using PCR Clean DX Beads (Aline Biosciences ...
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bioRxiv - Plant Biology 2020Quote: ... were amplified by PCR using PrimeSTAR GXL DNA polymerase (Takara) and gene-specific primer sets ...
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bioRxiv - Cell Biology 2020Quote: ... sgRNA inserts were PCR amplified using ExTaq DNA Polymerase (Takara). The resultant PCR products were purified and sequenced on a HiSeq 2500 (Illumina ...
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bioRxiv - Cell Biology 2020Quote: ... PCR was performed using SYBR Green Mix (Takara Bio, Japan). The primer sequences were shown in Table 1.
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bioRxiv - Genetics 2021Quote: ... Colony PCR was performed using Takara Taq polymerase (Clontech, TAKR001C). Plasmid DNA was isolated from cultured bacteria using QIAprep Spin Miniprep Kit (Qiagen ...
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bioRxiv - Neuroscience 2021Quote: ... or 1× Nested Universal Primer (secondary PCR, Clontech Laboratories, Inc.) and gene-specific primers ...
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bioRxiv - Developmental Biology 2020Quote: ... qRT-PCR was performed via SYBR Premix Ex Taq (Takara) and Rotor-Gene 3000 system (Corbett Research) ...
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bioRxiv - Plant Biology 2022Quote: ... RT-PCR was performed with PrimeSTAR GXL DNA polymerase (Takara) using gene-specific primers (Table S7) ...
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bioRxiv - Neuroscience 2022Quote: ... PCR reaction consisted of PrimeSTAR Max Premix 1x (Takara Bio), 0.4 μM of each primer and 30-100 ng DNA template ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR was conducted using PrimeSTAR Max DNA Polymerase from TaKaRa. The PCR products were checked by agarose gel electrophoresis and inserted into pET28a plasmid between the BamHI and EcoRI sites with Gibson Assembly method ...
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bioRxiv - Immunology 2019Quote: ... The RACE PCR products were cloned into pMD-19Tvector (Takara) and sequenced.
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bioRxiv - Neuroscience 2019Quote: ... The PCR product was InFusion-cloned (Clontech, catalogue number 639645) into the pBAC-ECFP-15xQUAS-TATA-SV40 plasmid7 (Addgene #104875) ...
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bioRxiv - Neuroscience 2020Quote: ... and then amplified by PCR using PrimeStar HS polymerase (Takara). Primers for amplifying the prenyl-Cdc42 coding sequence and 3’UTR were engineered to contain terminal Not I and Sal I restriction sites ...
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bioRxiv - Genomics 2021Quote: ... PrimeScript High Fidelity RT-PCR Kit (Takara Bio, Japan, R022A), In-Fusion Snap Assembly Master Mix (Takara Bio ...
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bioRxiv - Immunology 2021Quote: ... a semi-nested PCR was performed using PrimeStar MAX (Takara) for 35 cycles ...
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bioRxiv - Immunology 2021Quote: ... PCR was performed for 35 cycles using PrimeStar MAX (Takara). Primers used for these reactions can be found in Supplementary Table 1 ...
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bioRxiv - Cell Biology 2020Quote: ... PCR products were sub-cloned in pEGFP expression vectors (Clontech). KIF13B was amplified by PCR from GFP-KIF13B and inserted into a Bio-mCherry-C1 vector to make mCherry-KIF13B ...
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bioRxiv - Neuroscience 2021Quote: ... Viral titers were assayed by Lenti-X qRT-PCR (Clontech) and titers were adjusted to 1×108 copies/ml before injection.
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bioRxiv - Plant Biology 2021Quote: ... PCR amplified products (PrimeSTAR GXL DNA Polymerase, TaKaRa Bio Inc.) using primers and DNA templates listed in Table S4 were cloned into pLRE::LRE-cYFP plasmid linearized with SpeI-HF and AscI by using the In-Fusion HD Cloning Plus system (Clontech) ...
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bioRxiv - Microbiology 2020Quote: ... Real-time PCR was performed with 2x SYBR mix (Takara) in a QuantStudio-6 Flex Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Molecular Biology 2020Quote: ... then amplified by PCR with PrimeSTAR pre-mix HS (Takara), and cloned into pCR4Blunt-TOPO (ThermoFisher) ...
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bioRxiv - Cell Biology 2020Quote: ... PCR fragments were annealing in 1X prime star buffer (Takara) and Surveyor enzyme was added to digest the annealed DNA fragments at 42 °C for 1h ...
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bioRxiv - Microbiology 2022Quote: ... All PCRs were performed using PrimeSTAR Max polymerase (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... All PCRs were performed using PrimeSTAR Max polymerase (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... All PCRs were performed using PrimeSTAR Max polymerase (Takara Bio) per manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2020Quote: ... T4 ligase and KOD PCR kit were purchased from Takara Bio ...
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bioRxiv - Neuroscience 2020Quote: A multiple-step overlap-extension PCR (Pyrobest Polymerase, Takara Bio) was used for site-directed mutagenesis and construction of chimeric protein constructs ...
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bioRxiv - Microbiology 2021Quote: ... The PCR products were cloned into a pGEX6P2 vector (Clontech) which was subcloned the GFP gene at the N-terminus 48 ...
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bioRxiv - Microbiology 2022Quote: ... the GFP fragment was PCR amplified from pEGFP-N1 (Clontech) using specific primers to insert MluI/NotI restriction enzyme sites and ligated on those restriction enzyme sites into pCI-Neo (Promega) ...
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bioRxiv - Microbiology 2019Quote: ... digesting the PCR fragment with SacI (TaKaRa Bio Inc., Japan) and ligating again with Ligation high ver ...
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bioRxiv - Physiology 2019Quote: ... Real-time RT-PCR was performed using SYBR green (Takara) in BioRad CFX384 ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were cloned using the In-Fusion method (Clontech) into pGB135 digested with BamHI and EcoRI to generate plasmids pJRP33-34 ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were cloned using the In-Fusion method (Clontech) into pAMD-BA-lacZ digested with NheI and SphI to generate plasmids pJRP54-72 (see Table 2 for details).
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bioRxiv - Biochemistry 2019Quote: ... All DNA was cloned using backbone PCR and inFusion (Clontech). pHR vectors were used for mammalian cell experiments and pBAD vectors were used for bacterial protein overexpression ...
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bioRxiv - Developmental Biology 2020Quote: ... PrimeScript II High Fidelity One step RT-PCR Kit (Takara) was used with the following primers ...
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bioRxiv - Cell Biology 2021Quote: RT-PCR was conducted using PrimeSTAR Master Mix (Takara, Japan) according to manufacturer’s instructions along with PCR control ...
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bioRxiv - Neuroscience 2019Quote: ... Amplification was performed using Advantage GC 2 PCR kit (Clontech) and PCR products were cloned and sequenced.