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Citations for Takara Bio :
451 - 500 of 5144 citations for Human High Sensitive Interleukin 8 IL8 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... 293T cells were co-transfected with the aforementioned lentiviral vector plasmid and Lentiviral High Titer Packaging Mix (Takara Bio).
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bioRxiv - Microbiology 2023Quote: ... High-throughput qPCR (HT-qPCR) was performed by the Smart Chip Real-time PCR system (Takara Bio USA, Inc.). A non-template negative control was set for each primer set ...
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bioRxiv - Neuroscience 2021Quote: 8 DIV (days in vitro) neurons were transfected with 1.5 µg of pEGFP-N1 vector (Clontech Laboratories, Takara Bio Inc., Mountain View, CA, USA) or pEGFP-N1-TG2 vector (Furini et al. ...
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bioRxiv - Bioengineering 2024Quote: ... To synthesize cDNA, total RNA (8 µL, ca. 500 ng) was mixed with 2 µL of PrimeScript™ RT Master Mix (Takara Bio, Kusatsu, Japan) and incubated at 37°C for 15 min ...
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bioRxiv - Immunology 2022Quote: ... Seq Kit (Takara), as per the manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 kit (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... mRNA was retro-transcribed by Takara kit (Takara Bio), GAPDH was used as internal reference ...
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bioRxiv - Cancer Biology 2019Quote: ... A full-length Myc tagged cDNA expressing human NEK9 (NM_001329237.1) was subcloned into the pVLX-Tight-Puro vector (Clontech). The RCC1 domain was deleted using the Quickchange® II XL Site-Directed Mutagenesis Kit according to manufacturer’s instructions (Stratagene ...
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bioRxiv - Cell Biology 2022Quote: ... a DNA fragment was PCR-amplified from human genomic DNA using Tks Gflex DNA Polymerase (#R060A, Takara Bio) and the primers listed in Table S1 ...
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bioRxiv - Immunology 2023Quote: Wt or mutated human (h)ALPK1 cDNA constructs were cloned into the pCMV-myc plasmid (Takara Bio Inc) and were all siRNA resistant against the hALPK1 siRNA (s37074 ...
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bioRxiv - Cell Biology 2023Quote: ... Full-length human CLUH and mouse Cluh coding sequences cDNA were cloned in pcDNA3 and pmCherry-N1 (Clontech), respectively ...
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bioRxiv - Plant Biology 2019Quote: Full-length PC2 gene and its orthologs sequences with PR1 signal peptide sequence amplified from cDNA of various species using high-fidelity PrimeSTAR HS DNA Polymerase (Takara), primers pairs and restriction enzymes (Supplementary Table 4 ...
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bioRxiv - Microbiology 2019Quote: ... verticillioides strain M3125 cDNA using Q5® High-Fidelity DNA Polymerase and inserted in pGADT7 (Clontech, Mountain View, CA, USA) as prey vectors ...
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bioRxiv - Genomics 2021Quote: ... Target regions were amplified by PCR using specific primer sets and high-fidelity PrimeSTAR GXL DNA polymerase (Takara, Shiga, Japan). Sanger sequencing was performed using BigDye Terminator reactions and loaded onto a 3730xl DNA analyzer (Applied Biosystems ...
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bioRxiv - Microbiology 2019Quote: ... We conducted PCR across the gapped regions between the de novo assembled contigs with the primers described in S1 Table using the high-fidelity PrimeSTAR GXL DNA polymerase (Takara). The following PCR conditions were used ...
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bioRxiv - Cell Biology 2023Quote: Gene encoding ARF-1 were amplified from cDNA (cDNA synthesized from RNA isolated from Hela cells) using high fidelity DNA polymerase enzyme from TaKaRa and cloned with N-terminal flag and HA tag in mammalian expression vector pcDNA3 flag HA Akt1 plasmid obtained from addgene(1477) ...
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bioRxiv - Cell Biology 2023Quote: SseI gene was amplified from Salmonella typhimurium (STM) strain LT-2 strain using high fidelity DNA polymerase enzyme from TaKaRa and cloned in with N-terminal flag and HA tag in mammalian expression vector pcDNA3 flag HA Akt1 plasmid obtained from addgene(1477) ...
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bioRxiv - Microbiology 2023Quote: High-throughput qPCR was performed by an outside firm (Resistomap, Finland) using the qPCR SmartChip Real-Time PCR cycler (Takara). The qPCR cycling conditions and initial data processing were carried out as previously described by Wang et al ...
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bioRxiv - Microbiology 2024Quote: Each individual SARS-CoV-2 fragment was amplified from their respective plasmids using an exclusive pair of primers (Supplementary Table 2) and high-fidelity PrimeSTAR GXL DNA polymerase (Takara), followed by gel isolation with NucleoSpin Gel and PCR Clean-up (Macherey-Nagel ...
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bioRxiv - Microbiology 2024Quote: ... Y453F and T76G were inserted in plasmid F5 or F1 respectively by Directed Site Mutagenesis using the primers (Supplementary Table 2) and high-fidelity PrimeSTAR GXL DNA polymerase (Takara).
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bioRxiv - Developmental Biology 2021Quote: ... In-fusion HD kit and In-Fusion Snap Assembly kit (TaKaRa). pRSETa_mEos4b was a gift from Loren Looger (Addgene plasmid #51073 ...
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bioRxiv - Cancer Biology 2023Quote: ... LipofectamineTM 2000 kit and reverse transcription kit were purchased from TaKaRa Company ...
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bioRxiv - Cell Biology 2023Quote: ... Expression level of Venus and Venus-tagged arrestin-3 proteins was determined with anti-GFP JL-8 antibody (#632381, Takara Bio USA, San Jose, CA). The endogenous β-actin (loading control ...
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bioRxiv - Molecular Biology 2021Quote: ... was co-transformed with GAL4 DNA-BD-fused IPMK and a human brain cDNA activation domain (AD) library (Clontech). Two different reporter genes (HIS3 and ADE2 ...
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bioRxiv - Neuroscience 2020Quote: ... we used Sanger sequencing of cDNA reverse transcribed from pooled human hippocampus poly-A-selected RNA (Takara/Clontech 636165) to support the presence of the human-specific intron-3 retention event identified within APOE (GRCh38 ...
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bioRxiv - Neuroscience 2020Quote: ... we used Sanger sequencing of cDNA reverse transcribed from pooled human hippocampus poly-A-selected RNA (Takara/Clontech 636165) to support the presence of the human-specific intron-3 retention event identified within APOE (GRCh38 ...
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bioRxiv - Cell Biology 2021Quote: ... Gene expression profiles of mature hepatocytes were analyzed using human liver total RNA (636531, Clontech: Takara Bio, Shiga, Japan). Expression profiles of bile duct epithelial cells were obtained from Gene Expression Omnibus (GSM4454532 ...
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bioRxiv - Cell Biology 2021Quote: ... Gene expression profiles of mature hepatocytes were analyzed using human liver total RNA (636531, Clontech: Takara Bio, Shiga, Japan). Expression profiles of bile duct epithelial cells were obtained from Gene Expression Omnibus (GSM4454532 ...
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bioRxiv - Biophysics 2022Quote: Experiments were performed using PtK2 GFP-α-tubulin cells (stable line expressing human α-tubulin in pEGFP-C1, Clontech Laboratories ...
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bioRxiv - Cell Biology 2019Quote: Human PDE4D5 wild type and PDE4D5-dN mutant were PCR amplified and cloned into pLVX-mCherry-N1 vector (Clontech) using Gibson assembly after vector digestion with XhoI and EcoRI ...
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bioRxiv - Cell Biology 2020Quote: EGFP-Rab5 construct was generated by PCR amplifying human Rab5a from cDNA and subcloning into the pEGFP-C2 (Clontech) vector using XhoI/BamHI restriction sites ...
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bioRxiv - Microbiology 2019Quote: ... Sequences encoding primate PBMs were added to the resulting pDONR221-mCherry-human GBP1DC_BglII vector following BglII digestion using Ligation-Independent cloning (In-Fusion, Clontech) with annealed oligomers that also restored human GBP1 Q580-L581 and the human GBP1 CaaX box (Table S9) ...
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bioRxiv - Biophysics 2021Quote: ... PCR products were cloned into vector pcDNA3.3 (Thermo Fischer Scientific) for human IgG1 expression using In-Fusion cloning technology (Clontech) as described previously[1] ...
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bioRxiv - Immunology 2021Quote: ... Antibodies were produced by transient transfection of human epithelium kidney 293T Lenti-X cells (Clontech Laboratories, Mountain View, CA) using the JetPrime transfection kit (Polyplus Transfection Illkirch ...
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bioRxiv - Molecular Biology 2021Quote: ... Human eRF1 coding sequence was cloned into the XhoI and HindIII sites of the pλN-HA-C1 vector (Clontech). The pλN-HA-C1-eRF1 plasmid was then used to generate the pλN-HA-C1-eRF1AAQ (G183A G184A ...
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bioRxiv - Cell Biology 2021Quote: ... US) and human cervical adenocarcinoma cells expressing a tetracycline-inducible repressor (HeLa Tet-Off, Clontech, Mountain View, California, US) were cultured in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Biophysics 2021Quote: The EGFR-GFP plasmid was constructed using the cDNA of human EGFR (pNeoSRαII) provided by Akihiko Yoshimura (Keio University) and was inserted into the pEGFP-C1 vector (Clontech) with the same linker sequence suggested by Carter and Sorkin (Carter and Sorkin ...
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bioRxiv - Genomics 2023Quote: 200ng of RNA extracted from whole human blood used as input for reverse transcription using Smartscribe RT (Takara Clontech) with primers targeting the IGH constant regions (IDT ...
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bioRxiv - Genomics 2023Quote: 200ng of RNA extracted from whole human blood used as input for reverse transcription using Smartscribe RT (Takara Clontech) with primers targeting the IGH constant regions (IDT ...
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bioRxiv - Cancer Biology 2023Quote: ... Individual lentiCRISPRv2 vectors were introduced along with packaging vectors into human embryonic kidney (HEK) 293T cells via calcium phosphate transfection according to the manufacturer’s protocol (Clontech). Lentivirus was harvested at 48 and 72 hours after transfection in RPMI media supplemented with 10% FBS and filtered with 45 μm filters prior to transduction of cancer cell lines using centrifugation at 1000 g for 2 hours in the presence of 8 μg/mL of polybrene (Santa Cruz Biotechnology) ...
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bioRxiv - Cell Biology 2023Quote: ... Gene expression profiles of mature hepatocytes were analyzed using human liver total RNA (636531, Clontech: Takara Bio, Shiga, Japan).
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bioRxiv - Cell Biology 2023Quote: ... Gene expression profiles of mature hepatocytes were analyzed using human liver total RNA (636531, Clontech: Takara Bio, Shiga, Japan).
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bioRxiv - Biochemistry 2024Quote: ... The sequence of human histone H2A(K15C-129) was cloned into the pCold-Trigger factor (TF) vector (Takara Bio) with a SUMO coding sequence inserted to generate His6–TF–SUMO–H2A(K15C-129 ...
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bioRxiv - Plant Biology 2022Quote: The lethal gene ccdB flanked by BsmBI sites was inserted into the positions 245 and 246 of Eggplant latent viroid (ELVd) accesion AJ536613.1 and cloned into a pBluescript SK plasmid using high-fidelity PrimeSTAR HS DNA Polymerase (Takara, Kusatsu, Japan). This plasmid linearized with BsmBI was used to ligate self-hybridized oligonucleotides into the positions 245 and 246 of ELVd ...
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bioRxiv - Biochemistry 2021Quote: The proS gene was amplified from 50 ng population genomic DNA in a 25 μL PCR reaction using the high-fidelity polymerase PrimeSTAR Max DNA Polymerase (Takara Bio) and primers 134 and 138 (Supplementary Table 7) ...
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bioRxiv - Cell Biology 2021Quote: Previously described MEFs-IRE1-mNG and U2OS-IRE1-mNG cell lines (14, 27) were cultured in high glucose DMEM media supplemented with 10% tetracycline-free fetal bovine serum (FBS; Takara Bio), 6 mM L-glutamine ...
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bioRxiv - Microbiology 2023Quote: ... The ∼21.3 kb cps operon from galF through gnd was amplified in two pieces of overlapping amplicons with primers that also directed recombination with pMQ300 using PrimeSTAR GXL high fidelity polymerase (Takara Bio). Primers to amplify the DNA were 5470 (5’-ttgtgagcggataacaatttcacacaggaaacagctGTGAAGATGAATATGGCGAATTTG-3’ ...
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bioRxiv - Immunology 2024Quote: ... The RNeasy kit and the cDNA Synthesis Kit (Takara, Cat#: 9767, RR047A) was used to extract total RNA and prepare cDNA following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Protein samples were run on a 4-12% Bis-Tris gel (Novex) and Western blots were performed with the following antibodies: α-GFP (Clontech Living Colors 632381 (JL-8); RRID:AB_2313808 ...
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Diversified expression of gamma-protocadherins regulates synaptic specificity in the mouse neocortexbioRxiv - Neuroscience 2021Quote: LATaq Kit (RR002B, TaKaRa) was used in nested PCR ...