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Citations for Takara Bio :
451 - 500 of 2502 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... The PCR product was treated with DpnI (TaKaRa Bio) at 37 °C for 1 h and transformed into Escherichia coli JM109 ...
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bioRxiv - Microbiology 2023Quote: ... Viral cDNA was PCR amplified using CloneAmp HiFi (TaKaRa) in four overlapping amplicons of about 2.2 kb ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR product was ligated with InFusion ligation enzyme (TaKaRa) on EPB71 backbone (Addgene plasmid #90018 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... using the Advantage® 2 PCR Kit (Takara Bio) in a touchdown cycling program as follows ...
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bioRxiv - Molecular Biology 2023Quote: ... All the DNA templates were prepared by PCR (Takara) unless otherwise noted ...
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bioRxiv - Neuroscience 2023Quote: ... Clontech Advantage PCR buffer (1x) and mix (1x) (TaKaRa), linker-ligated DNA (0.6 v/v ratio) ...
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bioRxiv - Microbiology 2023Quote: ... PCR was performed using KOD DNA polymerase (Takara Bio) with the following settings ...
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bioRxiv - Developmental Biology 2023Quote: ... followed by PCR amplification using Ex-Taq polymerase (Takara) and template cDNA ...
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bioRxiv - Molecular Biology 2022Quote: We use a PCR amplification kit (Takara Bio, Japan) to obtain the purified dsDNA target and control with primers listed in Table 1 ...
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bioRxiv - Synthetic Biology 2022Quote: ... PCRs used the PrimeSTAR GXL DNA Polymerase (Takara Bio) under conditions following the protocol listed by the supplier ...
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bioRxiv - Microbiology 2022Quote: ... SMARTer® PCR cDNA synthesis kit (TaKaRa,cat 634926) and PrimeSTAR® GXL DNA polymerase (TaKaRa ...
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bioRxiv - Microbiology 2022Quote: ... and the SmartChip Real-Time PCR system (Takara Bio). The SmartChip MyDesign chip contains 5,184 nanowells ...
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bioRxiv - Developmental Biology 2023Quote: ... qPCR was performed using SYBR green PCR mix (TAKARA) in Roche Light Cycler.
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bioRxiv - Molecular Biology 2023Quote: ... mRNA levels were measured using qRT-PCR kit (Takara) in combination with TB Green real-time PCR ...
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bioRxiv - Microbiology 2024Quote: Polymerase chain reaction (PCR) was conducted using ExTaq (TaKaRa) to amplify the SLST locus ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and performed PCR using Tks Gflex DNA Polymerase (Takara) according to the manufacturing protocol.
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bioRxiv - Genetics 2024Quote: ... the mCherry sequence amplified by PCR from pmCherry (Clontech) was inserted into pBS-UASp (pBS-UASp-mCherry) ...
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bioRxiv - Genetics 2024Quote: ... Quantitative PCR was performed using SYBR Green (Takara, Japan) as described earlier (Singh et al ...
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bioRxiv - Biochemistry 2024Quote: ... The PCR product was cloned into EGFP-C3 (Clontech). Primers for mutagenesis were designed using NEBaseChanger (sequences shown in table S1 ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR reactions were assembled using ExTaq DNA polymerase (TaKaRa) according to the manufacturer’s suggestions ...
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bioRxiv - Microbiology 2020Quote: ... The amplification of viral DNA by PCR was carried out base on the manufacturer’s recommendations of the LA PCR Kit (TaKaRa Biomedical Technology Beijing, China). Briefly ...
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bioRxiv - Pathology 2022Quote: ... PCR was performed using Quick Taq HS DyeMix (Toyobo, Osaka, Japan) and a PCR Thermal Cycler Dice (TP650; Takara Bio Inc., Shiga, Japan), with the following cycling conditions ...
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bioRxiv - Microbiology 2020Quote: ... and qRT-PCR was performed on StepOne Plus Real-time PCR system (Applied Biosystem) with TB Green Premix Ex Taq II (Takara Cat no. RR820A). Briefly ...
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bioRxiv - Molecular Biology 2020Quote: ... Reverse transcription of the total RNA to complementary DNA and amplification of cDNA templates by long-distance PCR (LD-PCR) were performed using SMARTer Ultra-low Input RNA for Illumina Sequencing-HV (Clontech Labaratories, CA, USA) kit ...
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bioRxiv - Microbiology 2021Quote: RsmY and RsmZ expression quantification was done by RT-PCR using Mir-X miRNA qRT-PCR TB Green Kit (Takara Bio USA, Inc). For each reaction ...
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bioRxiv - Plant Biology 2020Quote: ... The band intensity of products obtained from semi-Q PCR was observed on the agarose gel and was further characterized with qRT-PCR using SYBR Green ® Premix (Takara Bio, USA). Specific primers were designed for studying expression of stress related genes in Arabidopsis and RP genes in rice using primer3 (v.0.4.0 ...
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bioRxiv - Microbiology 2023Quote: ... 5 μL of extracted RNA was subjected to one-step real-time RT-PCR using a One Step PrimeScript™ RT-PCR Kit (Perfect Real Time) (TaKaRa Bio Inc.) on a QuantStudio 5 Real-Time PCR system (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... The extracted RNAs were subjected to one-step real-time PCR using the One Step PrimeScript™ RT-PCR Kit (Perfect Real Time) (TaKaRa Bio Inc.).
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bioRxiv - Molecular Biology 2023Quote: ... was performed by quantitative real-time polymerase chain reaction (PCR) using the AAVpro™ Titration Kit (for Real Time PCR) Ver.2 (Takara Bio Inc), according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: Polymerase chain reaction (PCR) amplification of DNA receivers for plasmids construction and colony PCR were performed using PrimeSTAR® Max DNA Polymerase (R045B, TaKaRa, Kyoto, Japan). PCR primers were synthesized by Integrated DNA Technologies (IDT ...
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bioRxiv - Developmental Biology 2024Quote: ... were individually re-amplified using the 5’ PCR Primer II A with CloneAmp HiFi PCR Premix (Clontech Laboratories, Inc. A Takara Bio Company, #639298) by heating to 98°C x 2 min followed by 20 cycles of 98°C x 10 s ...
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bioRxiv - Genetics 2024Quote: ... Full-length cDNAs corresponding to SfVipR1 was PCR amplified using specific primers (Table 1) with reaction mixtures containing 25 μl 2×Gflex PCR buffer (Takara Bio, Shiga, Japan), 2 μl each of the sense and antisense primers (10 μM) ...
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bioRxiv - Cell Biology 2020Quote: ... the crude viral solution was concentrated using Lenti-x concentrator (Takara, cat.631232) to a final concentration of 108-109 IFU/ml using the Lenti-x qRT-PCR Titration Kit (Takara ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were pre-hybridised at 50 °C in ExpressHyb Hybridization Solution (Clontech, USA) for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... Hybridisation was performed overnight at 50 °C in ExpressHyb Hybridisation Solution (Clontech, USA). After hybridisation ...
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bioRxiv - Bioengineering 2020Quote: ... and lentiviral vectors were concentrated 50-fold using Lenti-X concentrator solution (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... After cross-linking membranes were pre-hybridised in ExpressHyb Hybridization Solution (Clontech, USA) at 40 °C for 1 h ...
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bioRxiv - Molecular Biology 2023Quote: ... media was removed and concentrated using lenti-X concentrator solution (Takara Bio #631232). Concentrated viral particles were distributed into single-use aliquots and stored at −80°C until use ...
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bioRxiv - Biochemistry 2023Quote: ... followed by incubation with Takara POD conjugate solution and DAB substrate (Takara, Japan).
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bioRxiv - Developmental Biology 2024Quote: ... Membranes were UV cross-linked and pre-hybridized with ExpressHyb Hybridization Solution (Clontech) for 1 h at 50°C ...
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bioRxiv - Neuroscience 2022Quote: ... where the lysing solution is composed of single-cell lysis buffer (Takara #635013): 1x RNase Inhibitor Murine (NEB) ...
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bioRxiv - Plant Biology 2022Quote: ... purified with a high-salt solution for precipitation (Takara Bio Inc., Ohtsu, Japan) and reverse transcribed with ReverTra Ace (Toyobo) ...
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bioRxiv - Neuroscience 2024Quote: ... The standard curve was prepared using the positive control solution provided by Takara with serial dilution for absolute quantification ...
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bioRxiv - Molecular Biology 2024Quote: ... Crosslinked membranes were prehybridized for ∼1 hour at 55C in hybridization solution (Takara). DNA oligos (Table S1 ...
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bioRxiv - Molecular Biology 2024Quote: ... were hybridized with specific 32P-labeled probes (Table S3) in ExpressHyb solution (Clontech) for 2 hours at 37°C ...
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bioRxiv - Bioengineering 2024Quote: ... Eluted solution was then loaded on TALON resin (Takara Bio Inc., Kusatsu, Japan) (bead volume ...
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bioRxiv - Immunology 2020Quote: ... cDNA was generated using a template□switch anchored reverse□transcription polymerase chain reaction (RT□PCR) based on the SMARTer pico cDNA PCR Synthesis Kit (Clontech, Mountain View, CA, USA). The final cDNA product was then subjected to a clean□up step using PCR Clean DX Beads (Aline Biosciences ...
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bioRxiv - Microbiology 2024Quote: ... the ORF4 region was amplified by RT-PCR with specific primers (Supplementary Table S2) using PrimeScript One Step RT-PCR Kit Ver.2 (Takara Bio Inc., Kusatsu, Japan), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... the 2nd PCR product was amplified from 10 ng purified 1st PCR product using the TaKaRa Ex Taq HS (TaKaRa Bio Inc., Japan; #RR006A) with a forward (5’-AATGATACGGCGACCACCGAGATCTACAC-Index2-ACACTCTTTCCCTACACGACGC ...
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bioRxiv - Plant Biology 2020Quote: ... were amplified by PCR using PrimeSTAR GXL DNA polymerase (Takara) and gene-specific primer sets ...