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Citations for Evrogen :
101 - 148 of 148 citations for 2' 3' cGAMP STING based FRET Detection Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... The libraries were sequenced on an Illumina NovaSeq 6000 platform (S Prime flow cell) with 2 × 150 bp paired-end reads (Evrogen Joint Stock Company, Russia).
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bioRxiv - Plant Biology 2021Quote: ... The cDNA for qRT-PCR was synthesized using an MMLV RT Kit (Evrogen, Russia) according to the manufacturer’s recommendations employing oligo(dT)17 -primers from 2 μg total RNA after DNase treatment ...
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bioRxiv - Immunology 2021Quote: Phagemid DNA was isolated from bacterial cells using the Plasmid miniprep kit (Evrogen, Russia). VHH-coding sequences were sequenced with Lac-prom (5’-CTTTATGCTTCCGGCTCGTATG-3’ ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCR fragments were gel-extracted using a Cleanup S-Cap kit (Evrogen, BC04). Next ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR reactions were conducted in duplicate with the Encyclo Plus PCR kit (Evrogen, # PK101) on a T100 Thermal Cycler (Bio-Rad ...
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Molecular coevolution of nuclear and nucleolar localization signals inside basic domain of HIV-1 TatbioRxiv - Evolutionary Biology 2021Quote: ... The membranes were blocked in 1% bovine serum albumin and incubated with either a monoclonal antibody against GFP (1:3000; Evrogen, Moscow, Russia) or a monoclonal antibody against B23 (1:10,000 ...
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bioRxiv - Cell Biology 2021Quote: ... Blots were incubated overnight at 4°C with primary antibodies targeting Tag(CGY)FP (1:2000 dilution in 1% milk, Evrogen, Cat#: AB121), HaloTag (1:1000 dilution in 0.5% milk ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated with the antibody (mouse anti-GFP [Thermos Fisher Scientific, 3E6, 1:500] or mouse anti-mKate2 [Evrogen, AB233, 1:500]) overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... and anti-tRFP (1:1000 Evrogen EVN-AB233-C100).
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bioRxiv - Molecular Biology 2020Quote: ... rabbit anti-TagRFP (Evrogen: AB233; RRID: AB_2571743; 1:1,000) and mouse anti-β-actin conjugated to HRP (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... supplemented with 1× Duplex-Specific Nuclease (DSN) buffer (Evrogen), slow-ramped (3°C/min ...
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bioRxiv - Molecular Biology 2020Quote: ... Reverse transcription was performed with random hexamer primers using the MMLV RT kit (Evrogen, Russia). The obtained cDNA was used as a template in RT-PCR ...
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bioRxiv - Molecular Biology 2023Quote: ... Uncleaved DNA fragments were extracted from the gel using the Cleanup Standard kit (Evrogen, BC04). These fragments were used as the templates for two-step PCR ...
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bioRxiv - Biochemistry 2020Quote: ... plasmid # 162785 Plasmids for cell transfections were purified by the Plasmid Midiprep kit (Evrogen, Moscow, Russia) and concentrated by ethanol precipitation in sterile conditions.
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bioRxiv - Immunology 2021Quote: ... The resulting NemR-cpYFP-bearing vectors were purified with the use of Plasmid Miniprep Kit (Evrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... or 1:1000 rabbit α-RFP (AB233, Evrogen, Moscow, Russia). The following secondary antibodies were then used at 1:5000 dilution ...
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bioRxiv - Cell Biology 2024Quote: ... Anti-tRFP (Cat#AB233, 1:1000) was purchased from Evrogen. Anti-CDK11A (Cat#ARP61814_P050 ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA bands were excised with subsequent purification of PCR products using a Cleanup Standard Kit (Evrogen, BC022). Restriction enzymes ...
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bioRxiv - Plant Biology 2023Quote: ... The PCR reaction was conducted using a high-precision polymerase Tersus Plus PCR kit (Evrogen, Moscow, Russia) with the following primers ...
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bioRxiv - Cell Biology 2023Quote: ... The RNA from each sample was then converted to cDNA using the MMLV RT kit (Evrogen, Russia) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: gDNA for T and CD45Δ T cells was obtained with an ExtractDNA Blood & Cells Kit (Evrogen, Russia) on the 2nd day after knockout ...
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bioRxiv - Neuroscience 2022Quote: ... 500-1000 μl of rabbit anti fRed (1:500; AB233, Evrogen) primary antibody was used per slice in a 2 ml Eppendorf tube ...
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bioRxiv - Zoology 2019Quote: ... and rabbit polyclonal for turboRFP/mKate2 (diluted 1:500; Evrogen AB234). Secondary antibodies (all diluted 1:2000) ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then incubated with anti-tagRFP (1:1,000, Evrogen, AB233), anti-SMI312 (1:1,000 ...
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bioRxiv - Systems Biology 2019Quote: Total RNA was extracted from the tissue fragments isolated by laser microdissection using an ExtractRNA Kit (Evrogen, Russia) (for details ...
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bioRxiv - Evolutionary Biology 2021Quote: ... or obtained by purification of salt method extracted DNA (Aljanabi & Martinez, 1997) using CleanUp Standard kit (Evrogen, Moscow). The dsDNA quantity was measured using dsDNA HS Assay Kit for fluorometer Qubit 3 (Life Technologies ...
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bioRxiv - Developmental Biology 2021Quote: An embryonic cDNA expression library was prepared from total RNA with a Mint cDNA synthesis kit (Evrogen, Russia) using the SMART approach ...
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bioRxiv - Cell Biology 2022Quote: ... Protein samples were detected with 1:1000 diluted anti-RFP (AB233; Evrogen) for BORR:RFP detection and 1:2000 diluted anti-Histone H3 (ab1791 ...
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bioRxiv - Cancer Biology 2024Quote: ... The following primary antibodies were used: mKate2 (Evrogen #AB233, 1:1,000, IF), SMAD4 (Millipore #04-1033 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... For polymer chain reactions we used Evrogen PCR kits with Hot Start thermostable polymerase (HS Taq polymerase, Evrogen, Russia). PCR reactions were carried out for each locus separately in a total volume of 14.1 μl (0.2 μl of a primer mixture containing forward and reverse primers ...
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bioRxiv - Plant Biology 2023Quote: ... We extracted total RNA from a 50-100 mg leaf tissue sample using the ExtractRNA kit (Evrogen, Moscow, Russia), based on TRIzol reagent ...
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bioRxiv - Biochemistry 2022Quote: An expression vector for the C-terminal 6×His-tagged ARD protein was constructed by amplifying the ard gene (GenBank Gene ID: 57822953) by PCR with a high fidelity Tersus PCR kit (Evrogen) and the primer pair 5’-GAGGAATAAATTTATGGCTCAGTTAGTCGAT / 5’-GGAAACAAGATCAGATGCACTCTC using the genomic DNA of V ...
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bioRxiv - Systems Biology 2022Quote: The first strand of cDNA was synthesized from a single-stranded RNA template using the MMLV RT kit (Evrogen, Russia) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... the corresponding gene was amplified with the use of the №17/№34 primer pair and purified with Cleanup Standard Kit (Evrogen). The obtained construct and intact PCS2+ vector were incubated with ClaI and XbaI FastDigest™ enzymes in the corresponding buffer (Thermo Scientific ...
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bioRxiv - Immunology 2021Quote: ... the target product was separated from the nontarget byproducts with horizontal DNA electrophoresis in agarose gel and purified with Cleanup Standard Kit (Evrogen). To engineer pQE30-NemR-cpYFP plasmids ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA for qPCR was synthesized using random primers and the First Strand cDNA synthesis kit (MINT Reverse Transcriptase, Evrogen, Russia) according to manufacturer’s instructions ...
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bioRxiv - Zoology 2023Quote: cDNA expression library was prepared by the SMART approach from total RNA with a Mint cDNA synthesis kit (Evrogen, Russia). cDNA gene fragments were isolated from the library by PCR with gene-specific primers ...
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bioRxiv - Genomics 2023Quote: ... 1.5 µl of dATP (10 mM) and 1 µl Encyclo polymerase (Evrogen, Moscow, Russia). The mixture was incubated at 37°C for 30 minutes followed by 5□min at 72□°C ...
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bioRxiv - Microbiology 2020Quote: ... The quantitative RT-PCR experiments were performed in triplicates on two independently grown colonies for each isolate in the sample with the use of OneTube RT-PCR SYBR Kit (Evrogen, Russia). The reactions were carried out on CFX96 Touch Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... The reverse transcription reaction was performed using reagents for RT with an OT-Random primer (Agrodiagnostika) and a kit of reagents MMLVRTkit (Evrogen, Russia), according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: cDNA expression library was prepared by the SMART approach from total embryonic RNA with a Mint cDNA synthesis kit (Evrogen, Russia). cDNA gene fragments were isolated from the library by PCR with gene-specific primers (see Table 1) ...
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bioRxiv - Cancer Biology 2019Quote: ... Primary antibodies were used: anti-GFP in a titer of 1:8000 (AB011, Evrogen, Russia), anti-NCL in a titer of 1:7000 (#a300-711A ...
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bioRxiv - Neuroscience 2020Quote: ... (ii) the same solution containing the primary antibody overnight at 4°C (anti-tRFP, 1:1000, Evrogen; anti-GABA ...
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bioRxiv - Cell Biology 2023Quote: ... and anti-Tag(CGY)FP (1:2000 in 0.5% milk TBST, 4°C o/n, Evrogen AB121). Blots were then incubated with IRDye 800/680 conjugated antibodies (1:10000 in 5% milk TBST ...
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bioRxiv - Neuroscience 2023Quote: ... was used to visualize LC neurons and rabbit anti-red fluorescent protein to visualise the fRed tag (tRFP, 1:1000, Evrogen). For visualisation of GRABNE2m expression in the hippocampus ...
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bioRxiv - Neuroscience 2019Quote: ... They were then incubated with primary antibodies, rat monoclonal anti-GFP (Nacalai Tesque, GF090R) at 1:2000 and rabbit polyclonal anti-tRFP (Evrogen, AB233) at 1:2000 ...
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bioRxiv - Microbiology 2020Quote: ... The fixed cells were harvested (8000 g, 5 min, 4 °C) and resuspended in 1 mL of ExtractRNA Reagent (Evrogen, Russia) and the subsequent procedures were performed according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... The DNA fragment encoding the RBDv1 ORF with Kozak consensus sequence and C-terminal c-myc and 6xHis tags were obtained by PCR using primers AD-COV-AbsF and AD-RBD-myc6HNheR (listed in Table 1) and Tersus polymerase mix (Evrogen, Moscow, Russia). Synthetic oligo’s ...