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Citations for Rockland :
151 - 184 of 184 citations for Recombinant Mouse Il2rg His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... The following secondary antibodies were used for immunoblotting: IRDye® 800-conjugated goat anti-mouse IgG (610-132-121; Rockland Immunochemicals ...
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bioRxiv - Biochemistry 2022Quote: ... The membrane was probed using mouse anti-FLAG antibody (Sigma, F3165; 1:1,000 in blocking buffer, Rockland Immunochemicals, MB-070) and rabbit anti-Tubulin (Cell Signaling ...
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bioRxiv - Biochemistry 2020Quote: ... TrueBlot secondary antibodies conjugated with horseradish peroxidase (anti-goat IgG, anti-mouse IgG and anti-sheep IgG, 1:1000 for western blot after immunoprecipitation; Rockland); Secondary antibodies conjugated with horseradish peroxidase (anti-mouse IgG ...
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bioRxiv - Neuroscience 2020Quote: ... Lysates were centrifuged at 15,000 rpm for 10 min and the supernatants were incubated with TrueBlot anti-rabbit or anti-mouse IP beads (Rockland) for 1 h (for preclearing) ...
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bioRxiv - Immunology 2020Quote: ... followed by blocking and two hours incubation in appropriate HRP conjugated secondary anti-mouse or anti-rabbit antibodies (TrueBlot antibodies, Rockland). Bound antibodies were detected using ECL-Plus reagents (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Wells were washed five times with PBS–Tween and then incubated with 50 µL of 1: 5,000 secondary antibody anti–mouse-HRP (610-1302, Rockland, USA) 1 h at 37 °C ...
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bioRxiv - Bioengineering 2023Quote: ... The sections were incubated with primary antibodies (Mouse anti-NeuN 1:1000, Abcam ab104224, lot GR3386324-1; Rabbit anti-RFP 647 nm 1:5000, Rockland 600-401-379 ...
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bioRxiv - Biochemistry 2023Quote: ... Horseradish peroxidase-conjugated anti-mouse or anti- rabbit IgGs were used as secondary antibodies (dilution 1:10,000; Rockland; Limerick, PA). β- ACTIN was used as a loading control ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were then incubated with HRP-conjugated secondary goat antibodies to mouse or rabbit diluted 1:7500 in PBST with 5% milk (Rockland) for 1 hour at room temperature ...
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bioRxiv - Biophysics 2024Quote: ... The membranes were then washed three times by incubating with fresh TBST for 10 min before incubation with anti-mouse IgG conjugated to a DyLight 800 fluorescent marker (Rockland) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue was then incubated overnight at room temperature on a rocker in the primary antibody (mouse anti-RFP, 1:500; Rockland) in NDS ...
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bioRxiv - Biophysics 2024Quote: ... slides were incubated overnight at 4°C in a humid chamber with primary antibodies in PBS-T and 2% milk: 1:500 mouse anti-H3K9me2 MABI0317 (MBL) and 1:500 polyclonal rabbit anti-RFP (Rockland). After washing 3 × 5 min with PBS-T ...
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bioRxiv - Neuroscience 2022Quote: ... Tissue was incubated for 24-hr in the primary antibody solution that consisted of the blocking solution and mouse anti-RFP (1:500; Rockland Immunohistochemicals). The following day ...
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bioRxiv - Biochemistry 2019Quote: ... appropriate infrared dye-coupled secondary antibodies (1:10,000 dilution of anti-rabbit IRDye800 and anti-mouse IRDye700, Rockland, Gilbertsville, PA, USA) were used and the blots were scanned in an Odyssey Imager (LI-COR Biosciences ...
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bioRxiv - Neuroscience 2020Quote: ... Next the cells were washed with tris buffer with 0.1% triton X and were incubated with secondary antibody goat anti mouse Cy3 (1:500, Invitrogen, Rockland, IL, USA) for Iba-1 and anti-GFAP (1:500 ...
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bioRxiv - Microbiology 2022Quote: ... The membranes were washed with TTBS and incubated with 1:5,000 goat anti-mouse HRP for 1 h at RT (Rockland 610-1302) in 5% milk-TTBS ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies were visualized using secondary Dylight™ 680 α-mouse antibodies or Dylight™ 800 α-rabbit antibodies (Rockland Immunochemicals). The Li-COR Odyssey Infrared Imaging system (Biosciences ...
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bioRxiv - Immunology 2023Quote: ... 10 μg/mL purified IgG or 10 μg/mL purified IgM (Mouse IgG and IgM whole molecule, Rockland, Limerick, PA, USA) for 1h at 37°C ...
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bioRxiv - Synthetic Biology 2019Quote: ... or hybridoma supernatant (approximately 20 μg/mL) diluted 1 in 2 with kinetics buffer followed by blocking with mouse IgG (Rockland, 010-0102) at 50 μg/mL in kinetics buffer ...
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bioRxiv - Biochemistry 2020Quote: ... Alexa 680 goat anti-mouse (1:10,000, Fisher A28183) and Dylight 800 goat anti-rabbit (1:10,000, Rockland labs 611-145-002), washed ...
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bioRxiv - Microbiology 2020Quote: ... Tween 20 and incubated for 1h at room temperature with 1:10,000 dilution of anti-mouse IgG-horseradish peroxidase conjugate (HRP) (Rockland Immunochemicals Inc., USA). Both primary and secondary antibodies were incubated in the presence of 5% milk (Sigma-Aldrich) ...
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bioRxiv - Genomics 2021Quote: ... the membrane was washed four times with 1xTBS 0.1% Tween-20 for 5min and then incubated with goat-anti mouse IgG IRDye680® (Rockland cat # 610144002) and goat anti-rabbit IgG IRDye800® (Rockland cat # 611132122 ...
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bioRxiv - Molecular Biology 2021Quote: Mono and oligo nucleosomes devoid of protein (salt stripped chromatin particles) were prepared from mouse embryonic fibroblasts (MEFs) derived in our laboratory and from chicken erythrocytes (Rockland R401-0050) as described (Ausio et al ...
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bioRxiv - Developmental Biology 2022Quote: ... Membranes were washed three times in PTW for 10 minutes each and then incubated with species- and isotype-specific horseradish peroxidase-conjugated secondary antibodies at 1:15,000 dilution (Neurog2: mouse IgG-HRP, Rockland, cat# 610-1302; NeuroD1: rabbit IgG-HRP, Rockland, cat# 611-1302) in blocking solution for one hour at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... The primary antibodies used were the mouse monoclonal anti-polyglycylated tubulin AXO49 (diluted 1:200) 52 and polyclonal anti-GFP antibodies (Rockland, 1:800). The secondary antibodies used were goat-anti-mouse IgG-FITC and goat-anti-rabbit-Cy3 antibodies (Jackson Immunoresearch) ...
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bioRxiv - Neuroscience 2021Quote: ... pH 7.5 and then incubated at room temperature for 2 hours on a rotary mixer at room temperature with 10 μg mouse monoclonal anti-GFP antibody (Rockland #600-301-215) diluted in 500 μl PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... washed and subsequently incubated with a horseradish peroxidase (HRP)-coupled mouse anti-goat secondary antibody (1:5000, #18-8814-33, Rockland Immunochemicals, Inc., USA) for an additional hour ...
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bioRxiv - Neuroscience 2023Quote: ... YFP was detected by incubating brains overnight at 4°C with a mouse monoclonal anti-GFP FITC antibody (1:300, Rockland 600-302-215). mCD8 RFP was visualized using native fluorescence ...
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bioRxiv - Immunology 2023Quote: ... the membrane was incubated with mouse serum (1:10 in washing buffer) or 5 μg/mL of purified IgG and IgM (Rockland, Limerick, PA, USA) in washing buffer overnight at 4°C under agitation ...
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bioRxiv - Microbiology 2023Quote: ... TNF-alpha and IL-10 in serum were determined using bead-based Multi-Plex kits (MILLIPLEX® Mouse Cytokine/Chemokine Magnetic Bead Panel 96-Well Plate Assay, Merck KGaA, Cat. No. MCYTOMAG-70K-03, Rockland, MA, USA). In order to remove particulates ...
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bioRxiv - Microbiology 2020Quote: ... The blocked membranes were probed overnight at 4°C temperature with a 1:5,000 dilution of mouse anti-6His antibodies (Rockland Immunochemicals Inc., USA or Thermo), rinsed three times with TBS-0.1% (v/v ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were washed 4X in the presence of 0.2% Tween-20 and then incubated with fluorescent anti-mouse (800nm, Rockland, 610-145-003, lot 34206) and anti-rabbit (680nm ...
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bioRxiv - Neuroscience 2023Quote: ... The primary antibodies used were 1:3000 mouse anti-PDF (Developmental Hybridoma Bank) and 1:1000 rabbit anti-RFP (Rockland, #600-401-379-RTU). After incubation ...
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bioRxiv - Cell Biology 2023Quote: ... This was followed by a one-hour incubation at room temperature with peroxidase-conjugated Rabbit or Mouse secondary antibodies (Rockland 610-1302 and 611-7302) at a dilution of 1:10000 ...