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Citations for Rockland :
1 - 50 of 140 citations for Rat Nuclear Factor Of Activated T Cells 5 NFAT5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The plates were developed with TMB (3, 3’, 5, 5’ - Tetramethylbenzidine) ELISA Peroxidase Substrate (Rockland Immunochemical, Limerick, PA), and absorbance was measured on a plate reader at 655 nm.
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bioRxiv - Microbiology 2023Quote: ... incubated for 1 hour at room temperature in TBS-T with 5% skim milk plus a 1:10,000 dilution of anti-rabbit DyLight 800 secondary (Rockland), and finally washed thrice for 5 minutes in TBS-T ...
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bioRxiv - Immunology 2020Quote: ... anti-rat IgG (Rockland Immunochemicals). Membranes were scanned using an Odyssey ® Infrared Imaging System ...
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bioRxiv - Cell Biology 2021Quote: ... Mouse (Rat) (18-8817-33, Rockland Immunochemicals Inc. ...
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bioRxiv - Biochemistry 2021Quote: ... Slides were washed with TBS-T (four 5-min washes) and incubated with primary antibodies (diluted in Rockland blocking solution; Online-only Table 1) overnight at 4°C with agitation on a rocking platform ...
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bioRxiv - Microbiology 2020Quote: ... were cultured in liquid EMJH medium to a cell density of ∼2 × 108 cells/mL supplemented with 120 mM NaCl and 10% rat serum (Rockland Immunochemicals, USA) to simulate the in vivo host environment and to induce virulence gene expression (59) ...
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bioRxiv - Cell Biology 2020Quote: ... and rat IgG (Rockland Immunochemicals, Limerick, PA). Inhibitors used include ...
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bioRxiv - Immunology 2022Quote: ... and rat immunoglobulins (IgGs) were purchased from Rockland Immunochemicals ...
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bioRxiv - Cell Biology 2023Quote: ... Goat anti-rat Atto594 (1:200; Rockland Immuno); Alexa Fluor 647 Phalloidin in methanol (1:40 ...
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bioRxiv - Neuroscience 2022Quote: ... ATTO 647 goat anti-rat (1:400, Rockland, #612-156-120), and Alexa Fluor 405 goat anti-mouse (1:500 ...
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bioRxiv - Molecular Biology 2024Quote: ... Fluorescent TrueBlot® Rat anti-Mouse IgDyLight™ 680 (1: 1000) (Rockland) was used when expected bands were of similar size to IgG in Co-IP experiments.
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bioRxiv - Immunology 2022Quote: ... incubated with secondary anti-Rat IgG HRP (1:5000, Rockland Ref. 612-1302) for 1 hour at RT ...
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bioRxiv - Immunology 2023Quote: ... IgG2a isotype control (RTK2758) and Rat IgG F(ab’)2 Biotin isotype control (Rockland) were used as a control of Fcrl5 staining ...
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bioRxiv - Immunology 2019Quote: ... lysate samples were probed with rat-anti-human Tubulin (0.9 ng/ml; Rockland, PA, USA), followed by goat-anti-rat secondary antibody-Alexa Fluor® 800 conjugate (0.33 ng/ml ...
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bioRxiv - Microbiology 2020Quote: ... Cell pellets were carefully enrobed in an equal volume of molten 5% low-melting temperature agarose (Lonza, Rockland, ME) and allowed to cool ...
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bioRxiv - Microbiology 2021Quote: ... Cell pellets were carefully enrobed in an equal volume of molten 5% low-melting temperature agarose (Lonza, Rockland, ME) and allowed to cool ...
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bioRxiv - Microbiology 2022Quote: ... cells were blocked at 37°C for 45 min in a solution of 5% normal goat serum (Rockland Immunochemicals) in PBS ...
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bioRxiv - Microbiology 2022Quote: ... Cell pellets were carefully enrobed in an equal volume of molten 5% low-melting temperature agarose (Lonza, Rockland, ME) and allowed to cool ...
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bioRxiv - Microbiology 2024Quote: ... Cell pellets were carefully enrobed in an equal volume of molten 5% low-melting temperature agarose (Lonza, Rockland, ME) and allowed to cool ...
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bioRxiv - Cell Biology 2024Quote: ... Mouse TrueBlot® ULTRA: rat Anti-Mouse IgG (Rockland 18-8817-33; IB at 1:1000), Anti-IgG (H+L ...
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bioRxiv - Genetics 2020Quote: ... All cell lines were tested for mycoplasma with MycoAlertTM Plus kit (Lonza, Rockland, ME) and were mycoplasma-free cells ...
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bioRxiv - Cancer Biology 2022Quote: ... p19/ARF (clone 5.C3.1, Rockland) and HSP70 (clone N6 ...
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bioRxiv - Immunology 2022Quote: ... wells were washed 3x with PBS-T and a secondary antibody for that species was added (i.e., HRP goat-anti-human IgG, Rockland Immunochemicals ...
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bioRxiv - Biochemistry 2021Quote: ... the microarray slide was incubated for 15 minutes in PBS containing 0.05% Tween 20 (PBS-T, pH 7.4) and blocked with Rockland Blocking Buffer (RL) (Rockland Immunochemicals) at room temperature (RT ...
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bioRxiv - Genomics 2022Quote: ... the peptide microarrays were incubated for 15 minutes in phosphate buffered saline supplemented with 0.05% Tween 20 (PBS-T, pH 7.4) and blocked for 30 minutes with Rockland Blocking Buffer (RL) (Rockland Immunochemicals) at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... The supernatant was assayed for PlGF concentration using an enzyme-linked immunosorbent assay (ELISA) per manufacturer instructions (Rockland Immunochemicals, Inc, Pottstown, PA). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... mouse-anti-p19/ARF (5.C3.1, Rockland); mouse-anti-AP4 (A-8 ...
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bioRxiv - Developmental Biology 2024Quote: ... to be blocked in 5% BSA (Rockland). Blots were then incubated with primary antibody overnight at 4°C and with secondary antibody for 1 hour at room temperature ...
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bioRxiv - Biophysics 2024Quote: ... slides were incubated overnight at 4°C in a humid chamber with primary antibodies in PBS-T and 2% milk: 1:500 mouse anti-H3K9me2 MABI0317 (MBL) and 1:500 polyclonal rabbit anti-RFP (Rockland). After washing 3 × 5 min with PBS-T ...
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bioRxiv - Immunology 2023Quote: ... in the presence of 22.5 μg/ml anti-Fcrl5 F(ab’)2 Biotin or Rat IgG F(ab’)2 Biotin isotype control (Rockland) and 20 μg/ml streptavidin (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2024Quote: ... membranes were washed with TBS-T and placed in an incubation with a fluorophore conjugated secondary antibody (Rockland Immunochemical, 1:10,000) in 1% NFDM with TBS-T for 1 hour ...
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bioRxiv - Neuroscience 2023Quote: Initial specificity assessment of purified pAb was done as follows: standard ELISA (enzyme-linked immunoassay) plates were blocked with 3% Fish Gelatin in PBS (Rockland Immunochemicals, #MB-066-100). Antigen was coated to a final concentration of 5 μg/ml in TMBE (Rockland Immunochemicals ...
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bioRxiv - Microbiology 2020Quote: ... and subsequently blocked with 5% goat serum (Rockland). After one hour ...
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bioRxiv - Developmental Biology 2023Quote: ... followed by an overnight incubation in a 1:100 dilution of CY3 conjugated anti-rat IgG antibody (612-104-120, Rockland). Samples were visualized by confocal microscopy (A1R+ Storm ...
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bioRxiv - Cancer Biology 2021Quote: ... All the cell lines were tested for mycoplasma using the MycoAlertTM Plus kit (Lonza, Rockland, ME, USA) and were mycoplasma-free.
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bioRxiv - Microbiology 2022Quote: ... Both cell lines were regularly monitored for mycoplasma contamination using the MycoAlert mycoplasma detection kit (Lonza Rockland). Primary CD4+ T cells were cultured in RPMI 1640 containing L-glutamine supplemented with 100 U/ml penicillin ...
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bioRxiv - Biochemistry 2021Quote: ... the membrane was washed with TBS-T and incubated with corresponding fluorophore-conjugated secondary antibody (Rockland Immunochemical RL611-145-002, 1:10000) in 1% non-fat milk/TBS-T for 1 h ...
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bioRxiv - Microbiology 2023Quote: ... the membrane was incubated in 0.2% milk dissolved in PBS-T with [HRP]-conjugated anti-rabbit IgG (1:40,000 dilution, Rockland cat# 18–8816-33). For RpoA western blots ...
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bioRxiv - Neuroscience 2023Quote: ... were blocked in 5% Normal Goat Serum (Rockland, Abcam) in 0.1% PBS TritonX ...
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bioRxiv - Physiology 2022Quote: ... B-cell marker; B220; #550286; BD Biosciences; with F[ab’]2 anti-rat IgG[H&L]; 1:500 dilution; 712-4126; Rockland antibodies& assays) and CD3 (1:500 dilution ...
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bioRxiv - Molecular Biology 2023Quote: ... Secondary antibodies used for chemiluminescence-based detection were 1:1,000 rat monoclonal anti-mouse Ig HRP (Clone eB144, Mouse TrueBlot ULTRA, Rockland #18-8817-30), 1:10,000 goat anti-rabbit IgG HRP-linked antibody (Cell Signaling Technology ...
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bioRxiv - Immunology 2022Quote: ... The cells were incubated overnight at 4°C with a rabbit antibody directed against the SARS-CoV nucleocapsid protein (Rockland; 1:1000 dilution in 5% goat serum), which cross-reacts with the SARS-CoV-2 nucleocapsid protein ...
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bioRxiv - Immunology 2023Quote: ... The membrane was then washed in PBS-T before incubation with horseradish peroxidase (HRP)-conjugated anti-rabbit IgG (IgG-HRP) (Rockland Immunochemicals, Pottstown, PA, USA) diluted 1:10,000 in PBS-T for one hour at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... and blocked with 5% normal goat serum (Rockland, Pottstown, PA) and 5% BSA (MilliporeSigma ...
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bioRxiv - Cancer Biology 2021Quote: ... Membranes were blocked with 5-10% non-fat milk (Rockland) in TBST and incubated with primary antibodies diluted in 5% non-fat milk TBST overnight on a 4°C shaker ...
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bioRxiv - Molecular Biology 2022Quote: ... then incubated in 5 mL Blocking Buffer (Rockland MB-070) for 1 hr at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... after which they were transferred onto PVDF membranes (Bio-Rad 1704156).The membranes were blocked using 5% BSA (Rockland BSA-50) in TBS-T buffer for an hour ...
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bioRxiv - Cell Biology 2023Quote: ... The PVDF membrane was blocked with 5% non-fat milk (Rockland) for 1-2h at RT ...
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bioRxiv - Neuroscience 2020Quote: ... All cells were routinely tested for mycoplasma contamination with MycoAlert Mycoplasma Detection Kit (Lonza, Rockland, ME, USA, Cat N° LT07-318). Chemicals and cell culture reagents were purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Cell Biology 2024Quote: ... or anti-CtBP2 (BE Biosciences, 612044, 1:500) followed by secondary antibody solution containing anti-Rat IgG DylightTM 488 antibody (Rockland, 612-141-120, 1:500) or anti-Rabbit IgG DylightTM 549 antibody (KPL ...