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Citations for Rockland :
51 - 77 of 77 citations for PCR Buffers since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: The dsDNA tagmentation was performed in 1xTD buffer [10mM Tris-Cl (pH 7.6, ROCKLAND, Cat.No.MB-003), 5mM MgCl2 (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... Plates were blocked for 1 hour in 200 µL assay buffer with 0.5% BSA (Rockland; BSA-50). Plates were washed 5x with assay buffer and incubated with 100 µL assay buffer with 0.5% BSA in combination with PBS or 1 µg of human soluble β-klotho at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... PDVF membranes were incubated overnight at 4°C with the following primary antibodies diluted blocking buffer (Rockland): Mouse anti APP A4 clone 22C11 (Millipore MAB348 ...
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bioRxiv - Cell Biology 2019Quote: ... Total proteins were extracted from pellets and using Radioimmunoprecipitation assay (RIPA) lysis buffer (Rockland, Gilbertsville, PA, USA) and the protein concentrations were quantified using the Bradford assay (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: ... Five washes with Wash Buffer preceded the deposit of 100 μL of anti-goat IgG antibody (Rockland) diluted 20,000-fold in blocking buffer and 1 h incubation at 37°C with agitation ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked with Rockland blocking buffer at room temperature for one hour (Rockland Immunochemicals, Pottstown, PA), incubated with primary antibody (diluted in Blocking Buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... in PBST and incubated with blocking buffer comprised of PBST with 3% BSA (BSA-50, Rockland Immunochemicals) and 10% goat serum (G9023 ...
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bioRxiv - Microbiology 2024Quote: ... The PCR product presence was confirmed with gel electrophoresis using the FlashGel® System (Lonza, Rockland, ME, USA). PCR products were then purified with the HighPrep® PCR kit (MagBio Genomics ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated in the same blocking buffer containing anti-red fluorescent protein antibody (polyclonal anti-RFP, Rockland, #600-401-379 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Membranes were blocked for 30 min with blocking buffer for fluorescent Western blotting (Rockland Immunochemicals, Inc., Pottstown, PA) and incubated with the primary antibody overnight at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... the microarray slide was incubated for 15 minutes in PBS containing 0.05% Tween 20 (PBS-T, pH 7.4) and blocked with Rockland Blocking Buffer (RL) (Rockland Immunochemicals) at room temperature (RT ...
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bioRxiv - Genomics 2022Quote: ... the peptide microarrays were incubated for 15 minutes in phosphate buffered saline supplemented with 0.05% Tween 20 (PBS-T, pH 7.4) and blocked for 30 minutes with Rockland Blocking Buffer (RL) (Rockland Immunochemicals) at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... Wells were mounted by pouring 1 ml of 2.5% melted agarose (50080, Lonza Rockland, ME in Danieau buffer (DBx1) into the μ-dish with the mold inside ...
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bioRxiv - Neuroscience 2023Quote: ... Blocking was performed in a solution containing four parts TBS (10 mM Tris Base pH 7.5, 150 mM NaCl) to one part Blocking Buffer (Rockland) for one hour ...
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bioRxiv - Biochemistry 2022Quote: ... The membrane was probed using mouse anti-FLAG antibody (Sigma, F3165; 1:1,000 in blocking buffer, Rockland Immunochemicals, MB-070) and rabbit anti-Tubulin (Cell Signaling ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were diluted in blocking buffer and added to spinal cords for incubation overnight a 4°C (1:800 of goat-anti-GFP-FITC, Rockland; 1:5,000 of rabbit-anti-RFP ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were diluted in blocking buffer and added to sections overnight at 4°C (1:400 for goat-anti-GFP-FITC, Rockland; 1:2,500 for rabbit-anti-RFP ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were blocked in PBS-T supplemented with 3% BSA and 2% donkey serum (blocking buffer) for 30 min before incubation with primary antibodies (GFP #A-11122 Invitrogen; RFP #600-401-379 Rockland; Actin #A5441 Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... Next the cells were washed with tris buffer with 0.1% triton X and were incubated with secondary antibody goat anti mouse Cy3 (1:500, Invitrogen, Rockland, IL, USA) for Iba-1 and anti-GFAP (1:500 ...
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bioRxiv - Molecular Biology 2022Quote: ... Subsequent incubation of other peptide microarray copies with survivin at a concentration of 1 μg/ml in incubation buffer was followed by staining with the secondary 6X His Tag Antibody DyLight680 (Rockland Immunochemicals) antibody and the monoclonal anti-HA (12CA5)-DyLight800 control antibody (Rockland Immunochemicals ...
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bioRxiv - Genomics 2022Quote: ... the fixed cell pallet was lysed in 100 µL Hi-C lysis The fixed cell pallet was lysed in 100 µL Hi-C lysis buffer (10 mM Tris-HCl pH=7.6 (Rockland, #MB-003), 10mM NaCl (Invitrogen AM9760G) ...
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bioRxiv - Synthetic Biology 2019Quote: ... or hybridoma supernatant (approximately 20 μg/mL) diluted 1 in 2 with kinetics buffer followed by blocking with mouse IgG (Rockland, 010-0102) at 50 μg/mL in kinetics buffer ...
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bioRxiv - Immunology 2022Quote: ... Microarray slides were initially incubated in a solution of 0.05% PBST (7.4 pH) and 10% blocking buffer (Rockland Immunochemicals, Inc., Pottstown, PA) for 15 minutes at 23°C ...
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bioRxiv - Neuroscience 2020Quote: ... A sample of the FACS input cell suspension as well as the sorted cells were lysed with 100 μl/100K cells of the lysis buffer described above and subjected to 10% SDS-PAGE and western blot with 1:500 anti-RFP (Rockland 8E5.G7) and 1:1000 anti-Bax antibodies as described above.
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bioRxiv - Physiology 2019Quote: ... The PCR products were mixed with loading dye and loaded on to a 1.0% SeaKem® GTG® agarose (FMC BioProducts, Rockland ME, USA) containing ethidium bromide ...
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bioRxiv - Cell Biology 2020Quote: ... and the nonspecific binding sites were blocked for an hour with a blocking buffer specially formulated for fluorescent western blotting (Rockland Immunochemicals, Pottstown, PA). Membranes were incubated overnight at 4 °C with the respective primary antibodies (Outlined in Table 2 ...
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bioRxiv - Immunology 2023Quote: ... the membrane was incubated with mouse serum (1:10 in washing buffer) or 5 μg/mL of purified IgG and IgM (Rockland, Limerick, PA, USA) in washing buffer overnight at 4°C under agitation ...