Labshake search
Citations for Lonza :
351 - 400 of 522 citations for Western Blot Strip It Buffer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Synthetic Biology 2023Quote: ... or BAFF-R (transposon, pCMV-hyPBase, SF buffer kit V4XC-2032, Lonza 4D Nucleofector, code DA100). CD79b1 refers to a chimeric protein comprising mouse CD79b extracellular domain with a CD28TM and truncated CD3ζΔ to promote surface expression in the absence of CD79a ...
-
bioRxiv - Cancer Biology 2023Quote: ... cell pellets were resuspended in two to three volumes of ACK lysis buffer (Lonza: #10-548E) according to the manufacturers’ specifications ...
-
bioRxiv - Immunology 2024Quote: ... an electroporation reaction consisted of 2 × 105 HEK293T cells in 20 μL of SF buffer (Lonza) and 2 μL of 20 μM Cas9 RNP (equivalent to 40 pmol final concentration) ...
-
bioRxiv - Pathology 2024Quote: ... passed through a 30 µm cell strainer and erythrocytes were lysed using ACK lysis buffer (Lonza). For analysis of DiD-loaded NPs internalization ...
-
bioRxiv - Immunology 2024Quote: ... using standard procedures including red blood cell lysis with ACK lysis buffer (Lonza by Thermo Fisher). Before cell staining ...
-
bioRxiv - Microbiology 2024Quote: ... and the pellet was resuspended in 20 µL of supplemented room-temperature P3 electroporation buffer (Lonza) per reaction ...
-
bioRxiv - Cancer Biology 2024Quote: ... 3ug of purified EVs were electroporated in 20μL of SF buffer (#V4XC-2032, Lonza, Basel, Switzerland) with 0.4μg of CRE-expressing plasmid (Addgene #172433 ...
-
bioRxiv - Genomics 2021Quote: ... PBMCs were then treated with 2 ml of erythrocytes lysis buffer (Lonza Walkersville inc. #120-02-070) for 1 min ...
-
bioRxiv - Neuroscience 2020Quote: The dissociated sensory neuron pellet was resuspended with 98 µL electroporation buffer (Mouse neuron nucleofector kit, Lonza) mixed with RNA oligos (0.2 nmol per transfection ...
-
bioRxiv - Biophysics 2021Quote: ... a 50 µL cell pellet was mixed with 100 µL electroporation buffer (SF cell line solution, Lonza) containing AMUPol and electroporated (HEK293 pulse sequence ...
-
bioRxiv - Genetics 2020Quote: ... and cells were resuspended in 20µL/reaction of room-temperature Lonza nucleofection buffer P2 (Lonza V4XP-2024). The cell suspension was gently mixed with 2.5µL/reaction of appropriate RNP and then pipetted into a 96-well-format nucleofection cuvette for the Lonza 4D X unit or Shuttle unit (Lonza) ...
-
bioRxiv - Immunology 2021Quote: ... Splenocyte suspension was spun down (1250 rpm, 5 min, 4C) and lysed in ACK Lysis buffer (Lonza) for 5 minutes on ice ...
-
bioRxiv - Microbiology 2021Quote: Each transfection reaction was prepared by adding 2 µl of “primed” cells resuspended in SG buffer (Lonza) to a mixture of ...
-
bioRxiv - Physiology 2020Quote: ... 50μl of blood was lysed using 500μl of ACK (Ammonium-Chloride-Potassium) Lysing Buffer (Lonza, Walkersville, USA) 10 min at room temperature ...
-
bioRxiv - Microbiology 2020Quote: ... and the pellet was resuspended in 20 µl of room temperature P3 electroporation buffer (Lonza, Basel, Switzerland) per reaction ...
-
bioRxiv - Microbiology 2020Quote: ... and the pellet was resuspended in 20 µL of room-temperature SE electroporation buffer plus supplement (Lonza) per reaction ...
-
bioRxiv - Immunology 2019Quote: ... T cells were centrifuged for 10 min at 90x g and resuspended in electroporation buffer P3 (Lonza) at a concentration of 750,000 cells/ 20 μL ...
-
bioRxiv - Biophysics 2019Quote: ... Approximately 1 to 1.5 × 106 hPSCs were washed in DPBS and resuspended in P3 primary buffer (Lonza) with 1 μg gRNA ...
-
bioRxiv - Immunology 2021Quote: ... 2 × 106 cells were suspended in 100 µL buffer (P3 Primary Cell 4D-Nucleofector X Kit, Lonza), mixed with 4 µg DNA and transfected using the 4D-Nucleofector program DN-100 ...
-
bioRxiv - Immunology 2021Quote: ... Bone marrow was collected and red blood cells were lysed with ACK lysing buffer (Lonza, LZ10-548E). Cells were passed through a cell strainer (70 μm pore size ...
-
bioRxiv - Immunology 2021Quote: ... Afterwards, they were resuspended in 20 µl / 106 cells ice-cold electroporation buffer (1M, or P3 (Lonza) as indicated) ...
-
bioRxiv - Immunology 2021Quote: ... RNPs were electroporated immediately after complexing into Tregs and T cells resuspended in supplemented P3 buffer (Lonza).
-
bioRxiv - Molecular Biology 2022Quote: ... 1 × 105 to 2 × 105 cells were collected and resuspended in 15 µL of nucleofection buffer (Lonza). For each reaction ...
-
bioRxiv - Genomics 2023Quote: ... into 96-well plates containing 5 μl modified freeze buffer (0.1% NP-40, 7.5. % DMSO, 42.5 % 2X Profreeze-CDM (Lonza) in PBS ...
-
bioRxiv - Cancer Biology 2023Quote: ... Both bone marrow and mammary gland were treated with ACK Lysing Buffer (10-548E; Lonza, Basel, Switzerland) to lyse red blood cells ...
-
bioRxiv - Immunology 2023Quote: ... 1e6 T cells were resuspended in 20 µl P3 electroporation buffer with P3 supplement (Lonza. #V4XP-3032) as per manufacturer instructions and combined with 3 µl RNP ...
-
bioRxiv - Biophysics 2023Quote: ... a 50 µL cell pellet was mixed with 100 µL electroporation buffer (SF cell line solution, Lonza) containing AMUPol and electroporated (HEK293 pulse sequence ...
-
bioRxiv - Bioengineering 2024Quote: ... 2.5×105-1×106 CD34+ cells were resuspended in P3 buffer (Lonza, Basel, Switzerland, cat.: V4XP-3032) with complexed RNPs and electroporated using the Lonza 4D Nucleofector and 4D-Nucleofector X Unit (program DZ-100) ...
-
bioRxiv - Bioengineering 2024Quote: ... Suspensions were centrifuged for 10 minutes at 300g and digested with ACK lysing buffer (Lonza, Walkerville, VA). The cells were then washed with PBS ...
-
bioRxiv - Genetics 2019Quote: ... Between 1×10^4 – 5×10^4 cells per condition were resuspended in 20μl of Buffer P3 (Lonza) per reaction and quickly added to the Eppendorf tube containing the Cas9 gRNA RNP complex ...
-
bioRxiv - Neuroscience 2022Quote: ... 20,000 cells were resuspended in 100 μL nucleofection buffer from Human Stem Cell Nucleofector™ Kit 2 (Lonza). Salsa6f-AAVS1 SHL plasmid Template (2 μg ...
-
bioRxiv - Bioengineering 2022Quote: ... spleen and lymph nodes were gently homogenized and red blood cells were lysed in ACK lysis buffer (Lonza). The remaining cells were washed by PBS with 2%FBS and filtered through with a 50ml falcon tube ...
-
bioRxiv - Immunology 2022Quote: ... 1 x 10^6 purified naive T-cells were resuspended in 15µL buffer P3 + 5µL Supplement 1 (P3 Primary Cell 4D-NucleofectorTM X Kit S; Lonza) and mixed with 2.7µL RNP in the 16-well strips provided in the kit ...
-
bioRxiv - Immunology 2022Quote: ... Suspension cells were centrifuged to remove the culture medium and resuspended in 100 μL P3 nucleofection buffer (Lonza), to which 6.5 μL of RNP were mixed in ...
-
bioRxiv - Cancer Biology 2019Quote: ... 1×105 cells were washed once with PBS and then resuspended in 21.5μl of SF or P3 nucleofection buffer (Lonza), for K562s and HUDEP-2s/HSPCs ...
-
bioRxiv - Cell Biology 2021Quote: ... Individual nuclei of G1 cells were sorted directly into 5 μL freezing buffer (50% PBS, 7.5% DMSO, and 42.5% 2X ProFreeze-CDM [Lonza]) in 96-well plates using a FACSJazz cell sorter (BD Biosciences) ...
-
bioRxiv - Physiology 2022Quote: ... Media is removed and the cell pellet is resuspended in 3mL of ACK lysis buffer (Lonza 10-548E) for 2 minutes ...
-
bioRxiv - Immunology 2023Quote: ... The MPRA vector library was nucleofected into TPP macrophages (5µg vector into 5×106 cells) in 100μl nucleofection buffer (Human Macrophage Nucleofection Kit, Lonza) using a Nucleofector 2b (program Y-011) ...
-
bioRxiv - Immunology 2023Quote: ... The neutrophil pellet was then cleared of remaining red blood cells using ACK lysis buffer (Lonza #10-548E). For subsequent experiments ...
-
bioRxiv - Cell Biology 2023Quote: ... The RNP complex was mixed with confluent 2i-grown ES cells re-suspended in P3 transfection buffer (Lonza) before being transferred to an electroporation microcuvette well (Lonza) ...
-
bioRxiv - Immunology 2023Quote: ... 1-10 million of pre-activated P14 cells were resuspended in 20 μl of P4 Nucleofection Buffer (Lonza). This cell suspension was then mixed with sgRNA/Cas9 and then incubated at room temperature for 2 mins ...
-
bioRxiv - Immunology 2024Quote: ... 1 - 2.5 × 106 Jurkat T cells were resuspended in EP buffer from the Nucleofector® Kit V (Lonza). Cells were combined with 1.5 μg of donor plasmid in a reaction volume of 100 μL and electroporated on the Amaxa® Nucleofector® II using pulse code X-005.
-
bioRxiv - Physiology 2024Quote: ... Proteins were separated by electrophoresis (ProSieve EX Running buffer 10x, diluted in H2O to 1x, (Lonza, Basel, CH); Precision Plus Protein WesternC Standards ...
-
bioRxiv - Cancer Biology 2019Quote: ... NSCs were harvested with Accutase and 2-3×106 cells were resuspended in 100µl mouse neural stem cell nucleofector buffer (Lonza) with 2µg plasmid DNA ...
-
bioRxiv - Genomics 2020Quote: ... and 5×104 cells were resuspended in 20 μL SF electroporation buffer prepared with SF supplement (Lonza, Basel, Switzerland). 3 μL RNP complex solution was mixed with the cells and the cells were nucleofected using program DJ-110 on a 4D-Nucleofector (Lonza ...
-
bioRxiv - Developmental Biology 2021Quote: ... 2 × 105 organoid single cells were re-suspended with Lonza P3 nucleofection buffer and 1 µl of pmaxGFP (Lonza) and transferred to 20 µl nucleofection cuvette (V4XP-3024 ...
-
bioRxiv - Cancer Biology 2020Quote: ... primary glioma cells were isolated and red blood cells were lysed using ammonium-chloride-potassium (ACK) lysing buffer (Lonza). Tumor cells were resuspended in flow buffer (Hank’s Balanced Salt Solution with Ca2+ and Mg2+ ...
-
bioRxiv - Neuroscience 2022Quote: ... culture conditions and characterization: The isolated epidermis was placed in a petri dish containing HBSS buffer (Lonza, # CC-5022) for 10 minutes at room temperature ...
-
bioRxiv - Cancer Biology 2022Quote: ... 3 µL of the sgRNA (total 300 pmol) was added to 12 µL of SE buffer (Lonza V4XC-1032). In another well ...
-
bioRxiv - Cancer Biology 2022Quote: ... Melanoma cells were resuspended at a cell density of 50,000 cells in 20 µL SF electroporation buffer with supplement (Lonza) and TILs were resuspended at a cell density of 1 million cells in 20 µL P3 electroporation buffer with supplement (Lonza ...