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Citations for Lonza :
1 - 50 of 844 citations for Vitamin K epoxide reductase complex subunit 1 VKORC1 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... 1% MEM vitamins (Lonza, cat# 13607C). Mesothelial and fibroblast cells were used for the experiments at early passages (1-3) ...
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bioRxiv - Cancer Biology 2023Quote: ... 1% MEM vitamins (Lonza, cat# 13607C). To isolate fibroblasts ...
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bioRxiv - Cell Biology 2023Quote: cRM Proteinase K (Lonza) and endoglycosidase H (NEB ...
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bioRxiv - Immunology 2020Quote: ... 1×106 cells per guide were electroporated with the corresponding RNP complex using Lonza Electroporation Kit V (Lonza). After 48 h ...
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bioRxiv - Immunology 2023Quote: ... 1×10e6 cells per guide were electroporated with the corresponding RNP complex using Lonza Electroporation Kit V (Lonza). After 48 h ...
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bioRxiv - Immunology 2024Quote: ... 1×106 cells per guide were electroporated with the corresponding RNP complex using Lonza Electroporation Kit V (Lonza). After 48 h ...
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bioRxiv - Cell Biology 2021Quote: Exocyst subunits and PIP5K1C were expressed in SF9 cells cultured in Insect-EXPRESS (Lonza) by baculovirus mediated expression ...
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bioRxiv - Neuroscience 2023Quote: Electroporation with freshly assembled RNP complex was performed on hiPSCs using the Human Stem Cell Nucleofector Kit 1 (Lonza) in conjunction with the Amaxa Nucleofector 2b system (Lonza ...
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bioRxiv - Cell Biology 2023Quote: ... were delivered as a ribonucleoprotein complex with the DNA donor template using a Nucleofector 2b Device and the Human Stem Cell Nucleofector Kit 1 (Lonza) following manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... was transfected into 1 million GMSM-K with Amaxa Cell Line Nucleofector Kit V (Lonza, Cologne, Germany) using Nucleofector II (Lonza ...
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bioRxiv - Cancer Biology 2024Quote: ... RNP complex was delivered into 1x10^5 T47D cells by 4D-Amaxa Nucleofector System (Lonza) using the SE Cell Line 4D Nucleofector X Kit (Lonza ...
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bioRxiv - Microbiology 2021Quote: ... were gel purified and the linear fragment flanked by the small subunit rRNA (SSU) homology regions was transfected into log-phase promastigotes using an AMAXA Nucleofector 4D (Lonza). Parasites (8 × 106 cells ...
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bioRxiv - Molecular Biology 2023Quote: ... The resultant riboprotein complex was introduced into cells by nucleofection (4D-Nucleofector X unit, Lonza bioscience), using the SF cell line kit and A549 cell program (CM 130) ...
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bioRxiv - Immunology 2023Quote: ... a ribonucleoprotein (RNP) complex was first formed by incubating 5µg of TrueCut Cas9 Protein V2 (Lonza) with 10µg of sgRNA for 10 min at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... ReN cells VM were electroporated with ribonucleoprotein complex using the nucleofector P3 kit (Lonza, V4XP-3032). Single cell colonies were generated by limited dilution in 96-well plates ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... RNA complex and Alt-R HDR Donor Oligos were transfected into HCT116 cells by electroporation (Lonza Nucleofector 96-well Shuttle System ...
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bioRxiv - Cell Biology 2023Quote: ... Electroporation of the RNP complex was carried out using the SF Cell Line 4D-Nucleofector kit (Lonza) and program CM-130 with 2.0×105 cells ...
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bioRxiv - Cell Biology 2023Quote: ... gRNA/Cas9 RNP complex along with SOX10 donor template plasmid were nucleofected into 3450 iPSC (Lonza Biosciences). After nucleofection ...
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bioRxiv - Immunology 2020Quote: ... 4 μl of the RNP-complex was added and transfected usine program CM150 of the nucleofection device (Lonza). After transfection cells were seeded in StemFlex™ medium (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... The RNP complex was mixed with confluent 2i-grown ES cells re-suspended in P3 transfection buffer (Lonza) before being transferred to an electroporation microcuvette well (Lonza) ...
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bioRxiv - Bioengineering 2023Quote: ... Electroporation of the RNP complex was performed using the Lonza 4D-Nucleofector (Lonza Group Ltd, Alpharetta, GA, USA) in P3 Primary Cell Solution with program DZ-100 ...
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bioRxiv - Molecular Biology 2020Quote: ... 1×106 EBdCas9 cells were transfected with the above RNP complex and 100μM of ssDNA (HDR)(Sup Table 8) using AMAXA electroporation kit (LONZA) and plated in TeSR media (supplemented with 5μM ROCKi) ...
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bioRxiv - Cancer Biology 2023Quote: ... 3uL of the total Cas9-RNP complex was added to 200,000 K562 cells resuspended in 18uL SF cell solution (Lonza) and subjected to nucleofection with the Lonza 4D nucleofectorsystem using manufactured specified conditions for K562 cells ...
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bioRxiv - Immunology 2023Quote: ... The precomplexed HDR-RNP complex was electroporated into primary human T cells as described above using P3 buffer (Lonza). The T cells were cultured in T cell media with 300 IU/ml IL-2 for 7-10 days ...
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bioRxiv - Immunology 2022Quote: ... IFNAR KO A549 cells were generated by CRISPR-Cas9 ribonucleoprotein (RNP) complex (IDT) transfection using the Nucleofector system (Lonza Bioscience). Specifically ...
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bioRxiv - Genetics 2022Quote: ... Cells were dissociated using accutase and 1 x 106 cells in P3 buffer were mixed with the RNP/template complex and nucleofected in large cuvettes (V4XP-3024 Lonza) using 4D-Nucleofector on program CA137 ...
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bioRxiv - Immunology 2022Quote: ... containing RNP complex and enhancer DNA (IDT, Cat.1075915) and under electroporation with EO-115 program of 4D-nucleofactor X Unit (Lonza). Electroporated T cells recovered overnight in IL7 and Il15 medium were then activated by anti-human CD3/CD28 beads (Thermo Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 pmol of the dsODN was mixed together with 100 µl homemade nucleofection solution to the RNP complex and electroporated using Nucleofector 2b (Lonza) with A23 program and 2 mm electroporation cuvettes.
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bioRxiv - Immunology 2022Quote: ... 4.4 μl gRNA-Cas9 complex was added to a 20 μl cell suspension in P3 buffer and transferred into a nucleovette (Lonza), placed on Amaxa Nucleofector and 96 well Shuttle (Lonza ...
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bioRxiv - Immunology 2022Quote: ... containing RNP complex and enhancer DNA (IDT, Cat.1075915) and under electroporation with CM-137 program of 4D-nucleofactor X Unit (Lonza). Electroporated T cells recovered overnight in IL7 medium were then activated by anti-mouse CD3/CD28 beads (at 1:1 ratio for two days and expanded in culture medium containing 5ng/ml hIL2 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were transfected with RNP complex and HDR templates by nucleofection with SF Cell Line 4D-Nucleofector X Kit (Lonza) using 20-ml Nucleocuvette Strips ...
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bioRxiv - Cancer Biology 2024Quote: CRISPR-mediated mutagenesis was carried out by transiently introducing CRISPR/Cas9 RNA-nucleoprotein complex into cultured NB4 cells with the 4D-Nucleofector system (Lonza). The sample preparation kit was Lonza SF Cell Line 4D-Nucleofector Kit S (Cat# V4XC-2024) ...
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bioRxiv - Immunology 2023Quote: CRISPR–Cas9 gene knockout was performed by transient Cas9/gRNA (RNP) complex electroporation using the P3 Primary Cell 4D-Nucleofector X Kit S (Lonza). On day 4 of culture ...
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bioRxiv - Cancer Biology 2023Quote: ... were resuspended in 4D-Nucleofector Solution and then mixed with the Cas9/sgRNA complex and electroporated using the 4D-Nucleofector X Unit (Lonza). All tumor cell lines were maintained in high-glucose DMEM supplemented with 10% (v/v ...
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bioRxiv - Genomics 2023Quote: ... 2.5e5 RPE1 CRISPRi cells were nucleofected with 3μM RNP complex using an SE Cell Line 4D X Kit S (Lonza Bioscience) on a 4D-Nucleofector (Lonza Bioscience ...
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bioRxiv - Microbiology 2023Quote: ... or 1×106 CEM-M7 Cas9 cells were transfected with the HiFi Cas9 Nuclease V3 (IDT)/gRNA complex (80 pmol/300 pmol) (Lonza) using a non-targeting or a GRN(5’-GCGATCCTGCTTCCAAAGATC-3’) ...
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bioRxiv - Genomics 2024Quote: A Cas9/sgRNA RNP complex and linear dsDNA donor were transfected into HCT116 cells using the Amaxa Nucleofector II device (Lonza) set for program D-032 with the Cell Line Nucleofector Kit V ...
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bioRxiv - Cell Biology 2024Quote: ... The RNP complex was mixed with 80,000 confluent 2i-grown mESCs re-suspended in P3 transfection buffer (Lonza V4XP-3032) before being transferred to an electroporation microcuvette well (Lonza V4XP-3032) ...
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bioRxiv - Cancer Biology 2024Quote: ... were annealed to Cas9 protein (IDT) and the ribonucleoprotein complex was transfected into SKOV-3 cells using a 4D-Nucleofector (Lonza). Bulk transfected cells were checked by flow cytometry for successful knock-down ...
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bioRxiv - Biochemistry 2024Quote: ... The RNP complex and electroporation enhancer (IDT) were electroporated into HiBiT-BRD4 Jurkat cells using an Amaxa-4D-Nucleofector (Lonza). Electroporated cells were transferred to medium with HDR enhancer (IDT) ...
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bioRxiv - Cell Biology 2024Quote: ... 0.3×106 live cells were resuspended with the RNP complex and 20 µL of P3 Primary Cell Nucleofector Solution (Lonza), following the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... GMSM-K or HEKn were electroporated with plasmid using the Amaxa Cell Line Nucleofector Kit (Lonza) and the Nucleofector II instrument (Lonza ...
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bioRxiv - Cancer Biology 2022Quote: ... and mixed by pipetting with the full gRNA-Cas9 complex mix previously assembled then added in the electroporation chamber well (Lonza, V4XP3032). Cells were electroporated with the program EO-100 using the Lonza Nucleofector ...
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bioRxiv - Systems Biology 2024Quote: ... MT2 and MDA-MB-231 cells with HLA-A,B,C CRISPR-Cas9 knockout were generated through electroporation of Cas9-sgRNA ribonucleoprotein (RNP) complex using 4D-Nucleofector (Lonza Bioscience). Three sgRNAs targeting HLA gene (HLA sgRNA#1 ...
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A CRISPR activation screen identifies FBXO22 as an E3 ligase supporting targeted protein degradationbioRxiv - Biochemistry 2023Quote: ... MDA-MB-231 and PC3 cells with FBXO22 CRISPR-Cas9 knockout were generated through electroporation of Cas9-sgRNA ribonucleoprotein (RNP) complex using 4D-Nucleofector (Lonza Bioscience). Two sgRNAs targeting FBXO22 gene (FBXO22 sgRNA#1 ...
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Loss of TMEM55B modulates lipid metabolism through dysregulated lipophagy and mitochondrial functionbioRxiv - Cell Biology 2024Quote: ... Cells were transfected with 5 μg negative control or Jip4-Cas9/sgRNA complex using Cell Line Nucleofector™ Kit T (VCA-1002, Lonza) with Amaxa Biosystems Nucleofector II ...
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bioRxiv - Physiology 2021Quote: ... were mixed with 1.49 μL of ssODN (100 μM) and Cas9 complex consisting of 18 μL SF 4D-nucleofector X solution + supplement1 (Lonza V4XC-2012), 6 μL of sgRNA (30 pmol/μL) ...
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bioRxiv - Cell Biology 2021Quote: Genes were knocked out using 1-2 synthetic sgRNAs (Synthego) in complex with spCas9 (Aldeveron 9214) that were electroporated into cells using a nucleofector system (Lonza V4XC- 1032) according to published methods (Hoellerbauer et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... were mixed with 1.49 µL of ssODN (100 µM) and Cas9 complex consisting of 18 µL SF 4D-nucleofector X solution + supplement1 (Lonza V4XC-2012), 6 µL of sgRNA (30 pmol/µL) ...
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bioRxiv - Genetics 2024Quote: ... We then mixed the assembled RNP complex with EGFP and/or mCherry donor vectors and delivered them into WTC11 i3N iPSCs using nucleofection (Lonza, VPH-5012). After nucleofection ...