Labshake search
Citations for Lonza :
51 - 100 of 137 citations for Urotensin II Related Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... LUHMES cells were transfected by Nucleofection in a Nucleofector II device (Lonza) using a Basic Nucleofector Kit for primary neurons (Lonza ...
-
bioRxiv - Cancer Biology 2022Quote: ... 0.5 × 106 cells were electroporated on a Nucleofector II device (program A032, Lonza) with 36 pmol SpCas9 Nuclease V3 ...
-
bioRxiv - Microbiology 2020Quote: ... siRNA was transfected with Amaxa cell line nucleofector kit and Nucleofector II (Lonza) according to the manufacturer’s protocol.
-
bioRxiv - Cell Biology 2022Quote: Nucleofection of hCEC cells was carried out using the Amaxa Nucleofector II (Lonza), optimized for the HCT116 cell line ...
-
bioRxiv - Developmental Biology 2020Quote: ... ESCs were electroporated using Nucleofector II (Amaxa) and mouse ESC Nucleofector kit (Lonza). Afterwards ...
-
bioRxiv - Developmental Biology 2021Quote: ... Nucleofection was performed with program B-016 on an Amaxa Nucleofector II (Lonza). Cells were then grown for ten days under selection by 1 μg/mL puromycin and 10 μg/mL blasticidin ...
-
bioRxiv - Molecular Biology 2021Quote: ... The RAP80-/- knockout cell lines were generated by electroporation (Lonza Amaxa II Nucleofector) of plasmids expressing sgRNAs (5′- ATTGTGATATCCGATAGTGAT-3′ and 5′-GTTCTGTCAGTGTGAAGAGG-3′ ...
-
bioRxiv - Cell Biology 2023Quote: ... HEK STIM1/2-/- were transfected via electroporation using the Amaxa Nucleofector II (Lonza) according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2023Quote: ... CREBBPR1446C gene-editing reagents were introduced by nucleofection (Nucleofector II, Kit R, Lonza) consisting of a gRNA/Cas9 RNP complex (IDT 1072532 ...
-
bioRxiv - Biophysics 2023Quote: ... Cells were electroporated using program X-001 on the Amaxa Nucleofector II (Lonza). Transfected cells were cultured in DMEM growth media without any antibiotics for 24-48 hrs and then selected for 10 days with 1ug/ml puromycin (Thermo Fisher #A1113803 ...
-
bioRxiv - Immunology 2023Quote: ... CTLs derived from LifeAct-GFP expressing pmel-1 mice were incubated with peptide-loaded and IFNγ-treated B16 cells cultured in phenol red-free DMEM (Lonza) supplemented with 10% FBS on stage at 37°C ...
-
bioRxiv - Immunology 2024Quote: ... and HLA-A2+ T2 cells (can be efficiently loaded with exogenous peptides as they lack the transporter associated with antigen processing) were cultured in RPMI 1640 media (Lonza) supplemented with 10% FCS (Merck) ...
-
bioRxiv - Immunology 2022Quote: ... bergei were transfected with the target plasmids using Amaxa parasite nucleofection kit II (Lonza), by employing the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2022Quote: ... The nucleofection was performed using an Amaxa Nucleofector II (Lonza Bioscience, program B-016). After selection with 1 μg/ml puromycin ...
-
bioRxiv - Immunology 2023Quote: ... 5x106 T- cell were electroporated (Amaxa Nucleofector II) in 100 µl electroporation buffer (Lonza) in the presence of Cas9-RNP and 2 µg of DNA-template ...
-
bioRxiv - Cancer Biology 2023Quote: ... HPAF-II cells were assessed for mycoplasma using the MycoAlert PLUS Detection Kit (Lonza). Cells were cultured in a Thermo Scientific Forma Steri-Cycle i160 incubator at 5% CO2 and 37°C for normal culture ...
-
bioRxiv - Molecular Biology 2023Quote: ... We then performed electroporation using the Amaxa® Nucleofector® II Device from Lonza with the mouse ESC (A-013 ...
-
bioRxiv - Immunology 2024Quote: ... 5×104 APC were seeded together with 1×105 CD4+ T cells in the presence of relevant peptides at 2.5 ng/ul diluted in X-VIVO 15 medium (Lonza, Basel, Switzerland) supplemented with 4 % human AB plasma (Innovative Research ...
-
bioRxiv - Cell Biology 2021Quote: Transient transfection was performed by electroporation using a Nucleofector II instrument (Lonza, Wakersville, MD, USA) and Lonza nucleofection kit V (Lonza ...
-
bioRxiv - Cancer Biology 2023Quote: ... and 50 mM mannitol)23 and program V-024 of an Amaxa Nucleofector II (Lonza). T cells were allowed to recover in complete RPMI-1640-sodium bicarbonate medium and transfection efficiency was evaluated after 24h by flowcytometry and/or western blotting ...
-
bioRxiv - Cell Biology 2023Quote: Human ESCs were nucleofected using an Amaxa nucleofector II in Nucleofector Solution L (all Lonza) using B-016 preset ...
-
bioRxiv - Cell Biology 2023Quote: ... The cells were electroporated in 0.2-cm gap electroporation cuvettes by Amaxa Nucleofector II (Lonza) using protocols pre-installed by the manufacturer.
-
bioRxiv - Cell Biology 2020Quote: ... and 7 mM Na2HPO4 in nuclease-free water) using either the Amaxa nucleofector system II (Lonza) under nucleofection program T-005 or the Amaxa 4Dnucleofector system (Lonza ...
-
bioRxiv - Neuroscience 2020Quote: ... RNP complexes were transfected in 500,000 cells with Amaxa Nucleofector II (Amaxa Biosystems, Lonza, Basel Switzerland) using the P-022 program according to manufacturer’s protocol and adding 1 ul of 100 μM Alt-R® Cas9 Electroporation Enhancer (IDT ...
-
bioRxiv - Cancer Biology 2022Quote: ... 4 μg guide RNA and 200 pmol ssODN using the Amaxa Nucleofector II (Lonza, Basel, Switzerland). Cells were recovered in growth medium for 24 hr and then sorted as single cells into 96-well plates by FACS ...
-
bioRxiv - Molecular Biology 2020Quote: ... For transfections HEK cells were transfected via electroporation unsing the Amaxa® Nucleofector II® (Lonza) according to the user manual ...
-
bioRxiv - Bioengineering 2021Quote: ... ii Quantification of monolayer hydraulic conductivity for two cell lines (Lonza, light blue; ScienCell, dark blue) and for two dextran molecular weights (MW) ...
-
bioRxiv - Cancer Biology 2022Quote: ... ii) Adherent cell monolayers at an 80% confluence were dissociated with different concentrations trypsin-EDTA (LONZA), 10X (0,25% v/v ...
-
bioRxiv - Cell Biology 2024Quote: ... was achieved using a Nucleofector II electroporator and the Cell Line Nucleofector Kit V (Lonza Biosciences). MEFs and MDA-MB-231 cells were stably modified using BFP and DN-KASH expression plasmids in a doxycycline-inducible Piggybac plasmid backbone (Addgene #187019) ...
-
bioRxiv - Biochemistry 2020Quote: ... 50 mM HEPES pH 7.3) using the X-001 program of an Amaxa Nucleofector II (Lonza, Switzerland) (Burkard et al. ...
-
bioRxiv - Cell Biology 2020Quote: ... using the Human CD34+ nucleofector kit and the Nucleofector II device (both from Lonza Group Ltd., CH) following manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... pX459-derived expression plasmids were transfected into MDCK cells by an Amaxa Nucleofector II (Lonza, Basel, Switzerland). The transfected cells were selected with 2.0 µg mL-1 puromycin ...
-
bioRxiv - Molecular Biology 2023Quote: ... according to the manufacturer’s instructions and the T-018 program of the Amaxa Nucleofector II device (Lonza). After nucleofection ...
-
bioRxiv - Cell Biology 2023Quote: Stable RPE1 cells (∼0.5×106) were electroporated with pmScarlet_peroxisome_C1 (or mScarlet-PTS1) (250 ng) using an Amaxa Nucleofector II (Lonza) in accordance with the manufacturer’s instructions using Ingenio electroporation solution (Mirus) ...
-
bioRxiv - Cell Biology 2019Quote: ... Recombinant plasmids were verified by Sanger sequencing and electroporated into E14 cells using the Nucleofector II platform (Lonza) (program A-013) ...
-
bioRxiv - Microbiology 2020Quote: ... Nucleofection was performed using the program X-001 for Jurkat T-cells (Amaxa™ Nucleofector™ II, Lonza). Cells were cultured in RPMI-1640 medium media supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Molecular Biology 2020Quote: ... using the Cell Line Nucelofector Kit V and the Amaxa Nucleofector II Device (Lonza Group AG, Basel, CH). Stable cells were selected for with 1mg/mL hygromycin B (Invitrogen ...
-
bioRxiv - Immunology 2023Quote: ... T cells were subsequently transfected by electroporation of RNP using setting E0115 of the Nucleofector II device (Lonza). Cells were cultured overnight at density of 3×106 cells/mL in complete RPMI supplemented with 5 ng/mL IL-7 and IL-15 to account for cell loss after electroporation (∼33%) ...
-
bioRxiv - Genetics 2019Quote: ... Hela cells were electroporated with plasmids using Ingenio electroporation kit (SopaChem) and the Nucleofector™ II/2b device (Lonza). Cells were harvested 24h post-electroporation ...
-
bioRxiv - Developmental Biology 2019Quote: ... were nucleofected into 1×106 pluripotent RUES2 cells using the B-016 setting on an Amaxa Nucleofector II (Lonza). Nucleofected cells were then plated as per maintenance conditions ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... and the cell pellet was transfected with 1.0 μg of cheriff eGFP using a Nucleofector IIS (Lonza, Basel, Switzerland) and Amaxa Basic Neuron SCN Nucleofector Kit (VSPI—1003 ...
-
bioRxiv - Biochemistry 2021Quote: ... then the ventricles were dissected and dissociated by serial digestion at 37°C with 0.44 mg/ml (6800 U) Worthington Type II collagenase (supplied by Lonza) and 0.6 mg/ml pancreatin (Sigma-Aldrich ...
-
Epigenetic Interaction between UTX and DNMT1 Regulates Diet-Induced Myogenic Remodeling in Brown FatbioRxiv - Physiology 2020Quote: SiRNAs or overexpressing plasmids were transfected into day 4 differentiated BAT1 brown adipocytes using Amaxa Nucleofector II Electroporator (Lonza) with an Amaxa cell line nucleofector kit L according to the manufacturer’s instructions (Lonza ...
-
Self-organised pattern formation in the developing neural tube by a temporal relay of BMP signallingbioRxiv - Developmental Biology 2023Quote: A total of 3-4µg of plasmid DNA was electroporated into 3-4x106 early passage ES cells using the Amaxa Nucleofector II (Lonza) programme A-023 ...
-
bioRxiv - Genomics 2019Quote: ... one million cells were mixed with 20 μg plasmids and 100 μL supplemented Nucleofector Solution V and electroporated with a Nucleofector II device (Lonza) using Program T-016 ...
-
bioRxiv - Microbiology 2020Quote: ... 3 x 107 bloodstream form cells were harvested by centrifugation and transfected with 5-10 μg of linearized plasmid DNA using an Amaxa Nucleofector II (Lonza) with program X-001 ...
-
bioRxiv - Molecular Biology 2019Quote: ... POLE3 and POLE4 gene knockouts were generated in RPE1-hTERT Flag-Cas9 TP53-/- cells by electroporation of LentiGuide-Puro or LentiGuide-NLS-GFP vectors using an Amaxa II Nucleofector (Lonza). C16orf72 gene knockout clones were generated in RPE1-hTERT Flag-Cas9 TP53-/- cells by transfecting sgRNA/Cas9 ribonucleoprotein complex using Lipofectamine CRISPRMAX Cas9 Transfection Reagent (Invitrogen) ...
-
bioRxiv - Genetics 2019Quote: ... Electroporation was performed using the Human Adult Dermal Fibroblast Nucleofector™ Kit and the Nucleofector™ II/2b device (Lonza). Both cell lines were harvested 72-hours post-transfection for qRT-PCR ...
-
bioRxiv - Cancer Biology 2019Quote: ... TXNDC17 gene knockouts were generated by electroporation of LentiGuide vectors (see Table S6) using an Amaxa II Nucleofector (Lonza, Basel). 24 h after transfection ...
-
bioRxiv - Biophysics 2021Quote: ... The cell suspension was transferred to an electroporation cuvette and electroporated using program X-001 on an Amaxa Nucleofector II (Lonza). Cells in the cuvette were mixed with 300 μl of DMEM ...