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Citations for Lonza :
301 - 347 of 347 citations for UDP β L Arabinofuranose since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Tightly synchronized schizonts were transfected using the Amaxa 4-D electroporator and P3 Primary Cell 4D Nucleofector X Kit L (Lonza) according to the protocol described by Moon et al ...
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bioRxiv - Immunology 2020Quote: Targeted gene’s expression was knocked-down in up to 5 x 106 cells using the P3 Primary Cell 4D-Nucleofector X Kit L (V4XP-3024, Lonza) with 90 µl P3 Primary cell solution and 100 pmol of corresponding si_RNA (resuspended in 10 ul RNAse-free H2O) ...
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bioRxiv - Molecular Biology 2020Quote: ... 2×107 epimastigotes were resuspended in 100μl of Tb-BSF buffer (Schumann Burkard et al., 2011) and submitted to the program U033 in AMAXA Nucleofector (Lonza). Twenty-four hours after transfection ...
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bioRxiv - Immunology 2021Quote: ... according to the manufacturer instructions and seeded at 5×105 cells/mL in RPMI-1640 media with L-glutamine (Lonza) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Biophysics 2022Quote: ... The transfected cells were then co-cultured with BHK-21 cells (ATCC CCL-10) transfected with VSV-G using the SE cell Line 4D-Nucleofector X Kit L (Lonza) and cultured until virus-mediated CPE was observed ...
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bioRxiv - Bioengineering 2022Quote: ... 90 μL of nucleofection solution (16.2 μL of Supplement solution mixed with 73.8 μL of SF solution from SF Cell Line 4D-Nucleofector™ X Kit L) (Lonza) was mixed thoroughly with the cell pellet ...
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bioRxiv - Immunology 2022Quote: ... we used SF Cell Line 4D-Nucleofector™ X Kit L (for Raw 264.7 cells) or SG Cell Line 4D-NucleofectorTM X Kit L (for NIH/3T3 cells; both from Lonza Inc.). Up to 5 × 106 cells were resuspended in 100 μl of nucleofection solution ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were resuspended in 90 µL of nucleofection solution (16.2 µL of Supplement solution mixed with 73.8 µL of SF solution from SF Cell Line 4D-Nucleofector™ X Kit L) (Lonza), transferred to the 15 µL RNP solution ...
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bioRxiv - Immunology 2023Quote: ... at bead-to-cell ratio of 1:1 in human IL-2 medium (X-VIVO 15, serum-free hematopoietic cell medium, with 2mM L-Glutamine and gentamicin (Lonza) supplemented with 5% Human AB serum (Valley Medical) ...
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bioRxiv - Microbiology 2023Quote: ... Swabs were immediately placed in 500 µL Eagles’ Minimum Essential Medium (MEM) with L-glutamine (Lonza, Cat. No. BE12-611F) containing 2x antibiotic-antimycotic and L-glutamine ...
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bioRxiv - Immunology 2022Quote: ... Cryopreserved PBMC (5 x 106/sample) were thawed in prewarmed RPMI-1640 media supplemented with L-glutamine (Lonza, Basel, Switzerland) + 10% FCS ...
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bioRxiv - Cell Biology 2023Quote: ... Five million BJAB or OCI-LY1 cells were transfected with 2 μg plasmid DNA using the SF Cell Line 4D-Nucleofector X Kit L (Lonza) and the AMAXA Nucleofector biosystem (BJAB ...
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bioRxiv - Developmental Biology 2023Quote: ... and co-transfected with an ssODN donor template containing the desired modification into H9/WA09 cells via nucleofection (Amaxa P3 primary cell 4D nucleofector X kit L, Lonza) using the manufacturer’s recommended protocol ...
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bioRxiv - Neuroscience 2023Quote: ... The cell pellet was used for nucleofection using the P3 Primary Cell 4D-Nucleofector X Kit L (Lonza, V4XP-3024), a 4D-nucleofector core unit and X unit (Lonza ...
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bioRxiv - Immunology 2023Quote: Brachial and inguinal lymph nodes were digested for 1h at 37°C under shaking in R2 buffer (RPMI-1640 medium containing L-glutamine (Lonza) plus 2% fetal calf serum (Dutscher) ...
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bioRxiv - Cancer Biology 2024Quote: ... The OCI-LY3 cell line was cultured in IMDM with 25 mM HEPES and 2 mM L-glutamine (Lonza; Switzerland), supplemented with P/S ...
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bioRxiv - Microbiology 2019Quote: ... 10 μL were loaded onto 10 % polyacrylamide gels and separated in Tris-Tricine buffer according to manufacturer’s instructions (ProSieve; Lonza, Basel, Switzerland). The gels were wet blotted (Mini Trans-Blot Cell ...
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bioRxiv - Immunology 2021Quote: ... The infected cells were incubated for 72 h in Dulbecco’s Modified Eagle Medium with L-glutamine (DMEM; Lonza, Walkersville, MD, USA) containing 2% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2021Quote: Primary human lung microvascular endothelial cells (HMVEC-L) were seeded at 5000 cells/cm2 in EBM-2 with 5% FBS and EGM-2 supplement (all products, Lonza Bioscience) until reaching complete confluence 14 ...
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bioRxiv - Developmental Biology 2020Quote: ... or a PMO Control at 100 µM in 100 µL solution from the P3 Primary Cell 4D-Nucleofector® X Kit L (V4XP-3024, Lonza) using the CB150 program on the 4D-Nucleofector™ System (Lonza) ...
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bioRxiv - Neuroscience 2019Quote: ... or a non-silencing plasmid immediately before plating (Amaxa™ P3 Primary Cell 4D-Nucleofector X Kit L; Lonza, CU133 program). The generation and knockdown efficiencies of shRNA plasmids were described previously (Guner et al. ...
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bioRxiv - Immunology 2021Quote: ... were washed in PBS/0.5%BSA and resuspended in 100 µl Nucleofector solution (Amaxa™ P3 Primary Cell 4D Nucleofector TM X Kit L, Lonza) with 4-6 µl of the appropriate siRNA (20-40 µM ...
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bioRxiv - Bioengineering 2022Quote: ... Deep Blue was prepared at a ratio of 1:10 with colourless DMEM (without pyruvate, with 4.5 g/L glucose (Lonza, Basel, Switzerland)) and was added to each existing well and incubated at 37 °C for 3 h ...
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bioRxiv - Neuroscience 2023Quote: ... 1 million cells were nucleofected with 1.2 µl of 20 µM Cas9-2NLS and 9 µl of 30 µM each sgRNA using Nucleofactor™ device (program CB1150, Lonza) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: Human isogenic ESCs containing different lengths of CAG repeats in the HTT Exon1 locus (HD-RUES2 6) were nucleofected using the Cell Line Nucelofector II (Kit L from Lonza, Walkersville, MD) by applying the B-016 program ...
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bioRxiv - Bioengineering 2019Quote: Nucleofection of the HDR template and CRISPR-Cas9 vectors was performed with SF Cell Line 4D-Nucleofection X Kit L (Lonza, V4XC-2024). Before nucleofection hybridoma cells were assessed for viability and centrifuged (90g ...
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bioRxiv - Systems Biology 2021Quote: ... we simulated the RPMI1640 culture medium following the nutrient availability provided by the formulation of RPMI1640 with L-Glutamine (Lonza, Basel, Switzerland), similar to the one reported in Folger et al. ...
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bioRxiv - Cancer Biology 2021Quote: ... AsPC-1 and CAPAN-1 pellets were suspended in Lonza transfection reagent (SE Cell Line 4D-Nucleofector™ X Kit L, Lonza, Germany) when the Mirus transfection reagent was finished ...
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bioRxiv - Microbiology 2022Quote: ... 1.2E6 BHK21 cells were nucleofected with 2 µg of Spike plasmid using an Amaxa Nucleofector II with cell line kit L (Lonza #VCA-1005) and program A-031 ...
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bioRxiv - Cancer Biology 2020Quote: ... 10 mmol/L HEPES (PAA Laboratory) and 1 ng/mL human basic fibroblast growth factor (bFGF) (Sigma-Aldrich) (Lonza, Walkersville, MD, USA). The generation number of ECs is maintained between 30-40 generations ...
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bioRxiv - Molecular Biology 2019Quote: ... and Ode macrophages (17) were cultured in RPMI 1640 medium supplemented with 2 mM of L-glutamine (Lonza, catalogue number 12-702F) and 10 mM Hepes (Lonza ...
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bioRxiv - Immunology 2021Quote: The Tap2-deficient T lymphoma cell line RMA-S (provided by Professor Klas Kärre, Karolinska Institutet) was cultured in RPMI-1640 medium supplemented with L-glutamine (Lonza, catalogue# 12-702F), penicillin-streptomycin (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... Vero cells obtained from the American Type Culture Collection (ATCC; Manassas, VA, USA; #CCL-81) maintained in Dulbecco’s Modified Eagle Medium with L-glutamine (DMEM, Lonza, Walkersville, MD, USA) supplemented with 2% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Immunology 2021Quote: ... gRNA_pX335 plasmids were transfected into EL4 cells by nucleofection using an Amaxa nucleofector and the Cell Line Nucleofector Kit L (Lonza, Basel, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... The cells obtained were cultured in complete medium (DMEM-F12, penicillin-streptomycin, L-glutamine, nonessential aminoacids, sodium pyruvate, all Gibco, Monza, Italy and 5% FBS, Lonza, Milan, Italy). Lung adenocarcinoma A549 was purchased by ATCC and cultured in DMEM-F12 supplemented with 10% FBS (All Gibco) ...
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bioRxiv - Cell Biology 2023Quote: PMOs were dissolved in distilled water and transfected into RD cells using the Amaxa Cell Line Nucleofector Kit L and a Nucleofector II electroporation device (Lonza, Basel, Switzerland) with program T-030 or into cells from a DMD patient without a transfection reagent.
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bioRxiv - Immunology 2023Quote: ... 80 µl of supernatant was removed and replenished with 100 µl IMDM/Glutamax/ 10% FBS containing 2× MycoZap Plus-PR (Lonza #VZA-2021), 100 ng/ml human IL-2 (GoldBio #1110-02-50) ...
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bioRxiv - Cancer Biology 2024Quote: ... CRISPR-CAS9 knockout in SU-DIPGXIII cells was performed using the Amaxa P3 Primary Cell 4D-Nucleofector X Kit L (Lonza, V4XP-3012). First ...
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bioRxiv - Cancer Biology 2021Quote: ... and CT-26 (ATCC #CRL-2638) 4T1 cells were cultured in DMEM or RPMI 1640 with L-Glutamine (Lonza™ BioWhittaker™ # BW12115F12) supplemented with 10% of FBS ...
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bioRxiv - Immunology 2020Quote: 3T3 cells were transfected with either hACE2 or GFP mRNA using an Amaxa cell line nucleofector L kit (Lonza; catalog number VCA-1005) according to the manufacturer’s instructions ...
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TIPRL1 and its ATM-dependent phosphorylation promote radiotherapy resistance in head and neck cancerbioRxiv - Cancer Biology 2023Quote: SQD9 cells were transfected with PX459 using Nucleofector™ 2b Device and Cell Line Nucleofector™ Kit L (Lonza, #AAB-1001, #VACA-1005). Cas9-expressing cells were selected with 0.625 µg/mL puromycin (Thermo Fisher ...
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bioRxiv - Immunology 2023Quote: ... were incubated with sgRNA and CAS9 protein complex and electroporation was done using P3 Primary Cell 4D-NucleofectorTM X Kit L (Lonza, cat # V4XP-3024) and Lonza 4D nucleofector system ...
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bioRxiv - Cancer Biology 2023Quote: Electroporation of multiplex CRISPR-Cas9 pX330 plasmids into normal bronchial epithelial cells was achieved using the P3 Primary Cell 4D-Nucleofector® X Kit L (Lonza, V4XP-3024) and 4D-Nucleofector™ X Unit (Lonza) ...
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bioRxiv - Genetics 2021Quote: Human embryonic microglia clone 3 (HMC3, ATCC® CRL3304™) cells were cultured in minimum essential medium (MEM) Eagle-EBSS with NEAA without L-Glutamine (Lonza, Cologne, Germany) supplemented with 10% fetal bovine serum (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: Embryonic monkey kidney cells (MA104) were grown in Dulbecco’s modified Eagle’s medium (DMEM) containing 4.5 g/L glucose (Lonza 12-640F or Corning 15-107-CV), 1% penicillin-streptomycin [Corning]) ...
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bioRxiv - Cancer Biology 2023Quote: ... 4T1 (ATCC #CRL-2539) and E0771 (CH3 Biosystems #940001) cell lines were culture in RPMI 1640 with L-Glutamine (Lonza™ BioWhittaker™ # BW12115F12) supplemented with 10% of FBS ...
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bioRxiv - Neuroscience 2021Quote: ... DRGs were then mechanically triturated and washed in a complete neurobasal A medium (NBA, Gibco® Invitrogen™ supplemented with 2% B27, 2mM L-glutamine and 1% antibiotics: penicillin + streptomycin, Lonza™ BioWhittaker™), before being plated in 35- mm petri dishes ...