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Citations for Lonza :
1 - 50 of 164 citations for Recombinant Mouse Cd34 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2019Quote: ... Human BM CD34+ cells (Lonza) were expanded for 5 days in Stemline II (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2021Quote: ... Cord blood CD34+ cells (2C-101, LONZA) were thawed according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... and Human CD34+ Cell Nucleofector Kit (Lonza) using a Nucleofector 2b Device (Lonza) ...
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bioRxiv - Bioengineering 2021Quote: ... using the Human CD34+ cell nucleofector kit (Lonza) with program U-008 ...
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bioRxiv - Developmental Biology 2019Quote: ... Human BM-CD34+ cells were purchased from Lonza (Tissue Acquisition Number ...
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bioRxiv - Cell Biology 2023Quote: ... CD34+ cells were resuspended in Opti-MEM (Lonza) at a concentration of 3-5×106 cells in a total volume of 750 μl ...
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bioRxiv - Molecular Biology 2020Quote: ... CD34+ cells were resuspended in P3 buffer (Lonza, Basel, Switzerland) with complexed RNPs and electroporated using the Lonza 4D Nucleofector (program DZ-100) ...
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bioRxiv - Bioengineering 2021Quote: ... CD34 and CD45 markers were received from Lonza (Allendale, NJ). Dulbecco’s modified eagle medium (DMEM) ...
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bioRxiv - Microbiology 2019Quote: ... and using the Amaxa human CD34+ cells Nucleofection Kit (Lonza), following the manufacturer’s recommendations ...
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bioRxiv - Bioengineering 2023Quote: ... and Amaxa Human CD34+ Cell Nucleofector kit (Lonza, Basel, Switzerland) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Human CD34+ HPCs isolated from full-term cord blood (Lonza) or adult bone marrow (Lonza ...
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bioRxiv - Immunology 2024Quote: ... human mobilized peripheral blood derived CD34+ cells (Lonza, 4Y-101C) were expanded in StemSpan SFEM II (StemCell Technologies ...
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bioRxiv - Genetics 2024Quote: ... CD34+ cells were resuspended in P3 buffer (Lonza, Basel, Switzerland) with complexed RNPs and subsequently electroporated using the Lonza 4D Nucleofector and 4D-Nucleofector X Unit (program DZ-100) ...
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bioRxiv - Immunology 2022Quote: Isolated CD34+ HSCs were cultured in X-Vivo-10 media (Lonza), IL-3 ...
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bioRxiv - Developmental Biology 2023Quote: Deidentified umbilical cord blood (UCB) CD34+ cells were purchased from Lonza. iPSC derived HSCs were obtained from cultures at day 22 of differentiation and sorted based on CD34+ by Miltenyi magnetic activated cell sorting (MACS) ...
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bioRxiv - Molecular Biology 2020Quote: ... CD34+ HSPCs were then electroporated in 100μl electroporation reaction buffer P3 (Lonza) with 30μg HiFi Cas9 and 16μg MS sgRNA (pre-complexed for 10 minutes at room temperature ...
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bioRxiv - Genetics 2020Quote: ... CD34+ HSPCs were resuspended in X-VIVO 15 medium (Lonza #04-380Q) containing 50 ng/ml recombinant human Flt-3 ligand (Peprotech #300-19) ...
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bioRxiv - Genomics 2020Quote: ... The CD34+ cells were thawed and put into X-VIVO 10 (Lonza) containing 1% Glutamax (Gibco) ...
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bioRxiv - Cancer Biology 2024Quote: ... Bone marrow CD34+ cells from adult healthy donors were obtained from Lonza laboratories and the bone bank of Cochin hospital ...
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bioRxiv - Molecular Biology 2022Quote: ... CD34+ HSPCs were thawed and cultured into X-VIVO 15 (Lonza, 04–418Q) supplemented with 100 ng ml−1 human SCF ...
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bioRxiv - Bioengineering 2022Quote: ... Electroporation of CD34+ HSPCs was performed with P3 nucleofection solution (Lonza, Basel, Switzerland) in the Lonza Nucleofector 4D (program DZ-100) ...
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bioRxiv - Genetics 2023Quote: ... CD34+ HSPCs were electroporated using the P3 Primary Cell X kit S (Lonza) according to manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2020Quote: ... Both T cells and CD34+ HSCs were cultured in X-Vivo 15 media (Lonza) supplemented with 10% human serum AB (Merck Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... bone marrow (BM) or G-CSF mPB-CD34+ cells were directly purchased from Lonza or Hemacare ...
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bioRxiv - Neuroscience 2019Quote: ... followed immediately by engrafting 1-5×104 human umbilical cord CD34+ stem cells (Lonza Incorporated ...
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bioRxiv - Cell Biology 2022Quote: ... BEL-A cells (1.0 × 106) were resuspended in CD34+ nucleofection kit buffer (Lonza Biosciences) containing 2.5 µg px458 (with gRNA ...
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bioRxiv - Genetics 2022Quote: ... CD34+ HSPCs were thawed on day 0 into X-VIVO 15 (Lonza, 04–418Q) seeded and maintained at a density of 0.5–1×106 cells per ml and supplemented with 100 ngml−1 human stem cell factor (SCF ...
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bioRxiv - Bioengineering 2024Quote: ... and recombinant human insulin 0.5%) (Lonza). Human cervical cancer cell line SiHa from ATCC (ATCC® HTB-35™ ...
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bioRxiv - Cell Biology 2022Quote: ... The plasmid pairs were co-electroporated into HUDEP2 cells using Nucleofector Kit CD34+ (Bioscience, Lonza) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... The complexes were added to 50,000 - 100,000 CD34+ HSPCs resuspended in 20 μL P3 (Lonza) and electroporated (program code DZ-100 ...
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bioRxiv - Cell Biology 2023Quote: CD34+ Cord Blood cells were obtained from de-identified healthy adult donors (Stemexpress, or Lonza). For mobilized peripheral blood CD34+ cells ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were electroporated in cuvettes (electroporation volume of 100 μL) using Human CD34+ Cells kit (Lonza) and U08 electroporation program ...
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bioRxiv - Cell Biology 2020Quote: ... using the Human CD34+ nucleofector kit and the Nucleofector II device (both from Lonza Group Ltd., CH) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... 2.5×105-1×106 CD34+ cells were resuspended in P3 buffer (Lonza, Basel, Switzerland, cat.: V4XP-3032) with complexed RNPs and electroporated using the Lonza 4D Nucleofector and 4D-Nucleofector X Unit (program DZ-100) ...
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bioRxiv - Bioengineering 2022Quote: A total of 500,000 CD34+ hiPSC-EPs were centrifuged twice and resuspended in Complete Endothelial Growth Media 2 (EGM-2, Lonza) supplemented with 50 ng/mL recombinant human vascular endothelial growth factor (VEGF ...
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bioRxiv - Cell Biology 2022Quote: A sub-cloned population of BEL-A cells (1.0 × 105) were resuspended in CD34+ nucleofection kit buffer (Lonza Biosciences) containing 18 pmol Cas9 ...
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bioRxiv - Bioengineering 2023Quote: ... Endothelial cells (ECs) were derived from CD34+ stem cells in endothelial cell growth medium (EGM; Lonza cat# CC-3162) supplemented with 20% fetal calf serum (FCS ...
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bioRxiv - Genetics 2020Quote: ... ∼1×106 cells were transfected with pairs of 5 µg gRNA plasmid and 4 µg ssODN (Supplementary Table 3) using AmaxaTM Human CD34 Cell NucleofectorTM Kit (Lonza) in the 2B-NucleofectorTM on the U-08 setting ...
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bioRxiv - Cell Biology 2022Quote: ... Two million of MNCs were transfected with 10 μg of plasmid (5:1 ratio pEB-C5:pEBTg) as instructed by the Lonza CD34+ nucleofector kit (VPA-1003, Lonza) and Amaxa nucleofector (program T-016 ...
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Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: ... CD34+ HSPC selected as previously described were preconditioned in SFEM II and StemSpan cytokines for 72 hours and electroporated with the Cas9-gRNP mixture using the Human CD34+ Cell Nucleofector kit (Lonza) in a Lonza IIb Nucleofector using program U-008 ...
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bioRxiv - Genetics 2023Quote: ... 2 day pre-stimulated CD34+ HSPCs were washed in PBS before being resuspended in P3 nucleofector solution (Lonza, V4XP-3032). An equivalent of 2.5×105 CD34+ HSPCs were mixed with gRNA:Cas9 complexes and 3 µg ssDNA donor template (20 μL ...
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bioRxiv - Cell Biology 2023Quote: ... BM CD34+ cells from young healthy donors for functional assays were collected at San Raffaele Hospital or purchased from Lonza. For the phenotypic analyses the sample size was determined by the number of individuals for whom excess material was available ...
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bioRxiv - Cancer Biology 2020Quote: ... recombinant Cas9 nuclease (IDT) and the 4D-Nucleofector (Lonza) as previously described (Wagenblast et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... human recombinant insulin (20 μg/mL, Lonza, #BE03-033E20), human recombinant EGF (2 ng/mL ...
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bioRxiv - Immunology 2022Quote: ... Co-cultures were done in IMDM supplemented with 5% HI-HS (Lonza) and gentamycin (86 μg/mL ...
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bioRxiv - Microbiology 2023Quote: ... 2 ×□106 unstimulated CD4+ T cells or 0.2 × 106 CD34+ cells were washed with PBS and resuspended in 20□μl buffer P3 (Lonza #V4XP-3032). To deliver two specific sgRNAs targeting one gene ...
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bioRxiv - Cell Biology 2022Quote: U2OS cells stably expressing GFP-tagged paxillin were cultured in phenol-red free DMEM (Lonza) at 37°C and 5% CO2 ...
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bioRxiv - Cancer Biology 2020Quote: ... The recombinant plasmid was transfected into HT29 cells using 4D-Nucleofecor (Lonza).
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bioRxiv - Biophysics 2020Quote: ... hydrocortisone and recombinant human epidermal growth factor (MEGM Bullet Kit CC-4136; Lonza). Cells were maintained in an incubator under standard conditions (36.5 °C ...
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bioRxiv - Bioengineering 2023Quote: The PyroGene Recombinant Factor C Endpoint Fluorescent Assay (Lonza, Walkersville, MD, cat # 50-658U) was performed as recommended ...